Acta Horticulturae Sinica

2026, Vol.53 No.9

Supervised by：China Association for Science and Technology

Co-sponsored by：Chinese Society for Horticultural Science and Institution of Vegetables and Flowers, Chinese Academy of Agricultural Science

Editor-in-Chief：Sun Rifei

Edited and Published by：Editorial Office of Acta Horticulturae Sinica

CN：11-1924/S

ISSN：0513-353X

Tel：010-82109523

E-mail：yuanyixuebao@126.com

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Genetic & Breeding · Germplasm Resources · Molecular Biology

Functional Study of the VaMYB4a-VaPAL Module in Response to Cold Stress in Vitis amurensis
LI Jiaxin, ZHANG Zhen, YU Qinhan, WU Wenkang, ZHANG Ningbo, XU Weirong
Acta Horticulturae Sinica. 2026, 53(9): 2531-2544. DOI:10.16420/j.issn.0513-353x.2025-0877
Abstract ( 0 ) HTML ( 0 ) PDF (3702KB) ( 0 ) 　　　
Low-temperature stress is a major abiotic factor limiting the growth and productivity of grapevine（Vitis spp.），yet the integrated mechanisms underlying the integration of cold signaling and metabolic regulation remain poorly understood. Here，a wild grapevine（Vitis amurensis）MYB transcriptionfactor（VaMYB4a）were characterized. Through the generation of VaMYB4a overexpression and CRISPR/Cas9 knockout lines，combined with physiological analyses，we found that VaMYB4a significantly enhanced the stability of photosystem Ⅱ，reduced membrane lipid peroxidation and reactive oxygen species accumulation，and thereby improved plant cold tolerance. qRT-PCR analysis showed that VaMYB4a activated the CBF-COR pathway and significantly induced the expression of CBF1-CBF4 and the downstream gene RD29A. Yeast one-hybrid，dual-luciferase reporter，and electrophoretic mobility shift assays demonstrated that VaMYB4a directly binds to the CAACCA cis-element in the VaPAL promoter and enhances its transcriptional activity，revealing a potential molecular mechanism by which VaMYB4a coordinately regulates the phenylpropanoid metabolic pathway. Overexpression of VaPAL markedly alleviated oxidative damage under low-temperature stress and enhanced cold tolerance in callus tissues. Promoter activity analysis further indicated that VaPAL is a key low-temperature-responsive gene with the highest sensitivity within the phenylpropanoid pathway. Co-expression analysis revealed that VaPAL can feedback-regulate the response intensity of the CBF pathway，thereby acting as a bridge between signal transduction and metabolic regulation. Collectively，the VaMYB4a-VaPAL module plays an important role in the coordinated“signal-metabolism”regulation of grapevine responses to low-temperature stress，providing valuable gene resources and a theoretical basis for the genetic improvement of cold tolerance in fruit crops.
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Genetic & Breeding · Germplasm Resources · Molecular Biology

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