Genome Wide Identification and Functional Characterization of Strawberry Pectate Lyases Related to Fruit Softening

doi:10.16420/j.issn.0513-353x.2024-0488

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (6): 1441-1450.doi: 10.16420/j.issn.0513-353x.2024-0488

• Genetic & Breeding?Germplasm Resources?Molecular Biology • Previous Articles Next Articles

Genome Wide Identification and Functional Characterization of Strawberry Pectate Lyases Related to Fruit Softening

FENG Jia, LU Peng, LI Li, YANG Li, and YANG Lei^*()

Shijiazhuang Pomology Institute，Hebei Academy of Agricultural and Forestry Sciences，Shijiazhuang 050061，China

Received:2025-03-21 Revised:2025-05-19 Online:2025-06-20 Published:2025-06-20
Contact: and YANG Lei

Abstract

Abstract:

Pectate lyase is associated with fruit softening. A total of 68 members of the pectin lyase family were identified and their chromosomal locations were mapped in cultivated strawberry（Fragaria × ananassa）. Pectate lyase members in Fragaria × ananassa，F. vesca，Malus × domestica，Prunus persica，Pyrus bretschneideri and Arabidopsis were divided into nine subgroups based on phylogenetic tree analysis.Transcriptome data analysis showed that the expression levels of Fxac_5g17410，Fxac_13g48930 and Fxac_26g11190 gradually increased with the development of‘Akihime’strawberry fruits，while the expression level was very low in‘Hongxing’strawberry with hard fruits. In addition，overexpression of Fxac_13g48930 in‘Hongxing’reduced fruit firmness，suggesting that Fxac_13g48930 may promote softening of strawberry fruits.

Key words: strawberry, fruit firmness, pectate lyase, soften

FENG Jia, LU Peng, LI Li, YANG Li, and YANG Lei. Genome Wide Identification and Functional Characterization of Strawberry Pectate Lyases Related to Fruit Softening[J]. Acta Horticulturae Sinica, 2025, 52(6): 1441-1450.

Figures/Tables 7

Table 1 The primer sequences for qRT-PCR

目的基因 Gene	引物序列（5′-3′） Primer sequence
FxaC_5g17410	F：TCAATGGCAGACAGGTCCAA；R：TCCCAATTCTCTTCTCGCGG
FxaC_13g48930	F：GAAATGGATTACGGCCTGCG；R：CTCCGTTCCACCATTCCTGA
Fxac_26g11190	F：TGCAGCCTTCGTGACTATGA；R：ACACGAGGTTGCCTTTGTCT
Actin	F：CGAGGCTCAATCCAAAAGAG；R：TGGCCACATACATAGCAGGA

Fig. 1 Firmness（A）and cell morphology（B）of strawberry fruits at different developmental stages ** P ≤ 0.01，t-test

Fig. 2 Distribution of pectin lyase in chromosomes of strawberry

Fig. 3 The evolutionary tree of pectin lyase gene family

Fig. 4 Collinearity analysis of pectin lyase gene family The red lines represent pectin lyase gene collinearity between species，and the gray lines are collinearity of all gene members

Fig. 5 Expression patterns at different stages of fruit development for differential genes of the pectin lyase family G：The green stage；T：the turning stage；R：The red stage

Fig. 6 The relative expression（A） and the fruit hardness（B）of three pectin lyase genes after overexpression ** P ≤ 0.01，t-test；Different lowercase letter indicates significant difference，P ≤ 0.05 level，Tukey’s test

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Genome Wide Identification and Functional Characterization of Strawberry Pectate Lyases Related to Fruit Softening

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