研究论文

• Effects of Phosphorus Levels and VA Mycorrhizae on Growth and Mineral Contents of Apple Seedlings
• Wang Chunmei;Han ZhenHai;Li Xiaolin;and Xu Xuefeng
• Acta Horticulturae Sinica. 2001, 28(1): 1-6.
• Abstract ( 1394 ) HTML ( 1158 ) PDF (125KB) ( 1158 ) 　　　
• Effects of 3 P levels in the side-room and / or infection of VA mycorrhizae on growth and contents of P , Fe , Zn , and Cu of Malus xiaojinensis seedlings were analyzed by employment with 3-rooms pot culture. The results showed that infection of VA mycorrhizae could significantly increase the seedling growth and improve their P nutrition. Iron contents in both root and the aboveground parts of the seedlings were remarkably increased , although Fe concentrations in the seedlings were significantly decreased due to P applications in the side-room. Zinc or Cu contents in the seedlings were also increased with the P applications.
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• Tropical Wild Fruit Germplasm in Xishuangbanna
• Ren Xinjun;Wen Bin;and Chen Guiqing
• Acta Horticulturae Sinica. 2001, 28(1): 7-11.
• Abstract ( 1595 ) HTML ( 1214 ) PDF (104KB) ( 1214 ) 　　　
• There are lots of fruit-edible wild trees , shrubs and vines in the tropical rainforest of Xishuangbanna. The biological characters of 35 main species whose fresh fruits are edible were studied , including special flavor , size , shape , blooming time and harvesting time. Some of them can be spread in the subtropics as cultivated varieties. The utility of important fruits is briefly intro-duced. The nutrition ingredients of 11 common fruits are analyzed. The thesis points out that the fruit plant resources have to be preserved and protected because of the rainforest degeneration and that study on acclimatization of wild fruits there should be carried out .
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• Genetic Transformation of the Trichoderma Endochitinase Gene ThEn-42 to Somatic Embryos of English Walnut
• Tang Haoru;Wallbraun M.;Ren Zhenglong;Reustle G. M.;and Krczal G.
• Acta Horticulturae Sinica. 2001, 28(1): 12-18.
• Abstract ( 1606 ) HTML ( 1429 ) PDF (145KB) ( 1429 ) 　　　
• Somatic embryos of English walnut (Juglans regia L.) were Agrobacterium-mediately transformed with the Trichoderma endochitinase gene ThEn242 under the control of a double 35 S CaMV promoter and Alfalfa Mosaic Virus leader sequence. The selectable marker gene neomycin phospotransferase Ⅱ (npt Ⅱ) driven by the nopaline synthase promoter was also used. A total of 41 putatively transformed somatic embryo lines were evaluated for expression of the npt Ⅱand ThEn-42 genes by PCR and multiplex PCR. All of the 41 somatic embryo selections expressed the npt Ⅱgene, among which 38 somatic embryo selections expressed the ThEn-42 gene. Analysis of chitinase activity by using a fluorometric assay showed dozens to 1 000-fold higher chitinase activity in transformed somatic embryos than that in non-transformed ones. All of the tested chitinase-postive somatic embryo lines analyzed by Southern blotting had an intact copy of the ThEn-42 gene. Chitinase expressing somatic embryos were further germinated and are being propagated for disease resistance assays.
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• Studies on the Plant Regeneration from the Micro-Cross Sections ofBanana
• Huang Xia;Huang Xuelin;Wang Honghe;and Li Xiaoju
• Acta Horticulturae Sinica. 2001, 28(1): 19-24.
• Abstract ( 1511 ) HTML ( 1142 ) PDF (233KB) ( 1142 ) 　　　
• The organogenesis of ‘Guangdong 631’banana by micro-cross sections culture and the factors influencing plant regeneration were investigated. The optimal culture condition was established by a statistical method and making research on the single factor : (1) the medium for direct organogenesis contained Ma (MS modified) , BAP 10μmol/ L , KT 50μmol/ L and IAA 1μmol/ L ;
  (2) the medium for callus induction contained Mb (B₅ modified) , dicamba 10μmol/ L , IAA 1μmol/L , 2 , 4-D 0. 7μmol/ L , NAA 0. 5μmol/ L , BAP 4. 4μmol/ L and 1 g/ L activated charcoal ; (3) the medium for callus subculture contained Mb , 5μmol/ L dicamba , IAA 1μmol/ L , 2 ,4-D 0. 4μmol/ L , BAP 22μmol/ L , KNO₃ 500 mg/ L , vitamine 40 mg/ L B₁ and activated 0. 5μg/L charcoal ; (4) the medium for induction shoot buds from the callus was composed of halfstrength Ma , BAP 1μmol/ L , 0. 5μmol/ L IAA and 1 g/ L activated charcoal ; (5) the plant regeneration medium was composed of Ma , BAP 1μmol/ L , KT 4. 6μmol/ L and NAA 1μmol/ L. The callus induction and subculture was kept in darkness. The application of micro-cross sections of
  banana for regeneration of plants might be a useful alternative to cell suspensions and protoplast culture in banana transformation.
  
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• Influence of pHand Temperature on the Stability of Anthocyanin fromLitchi Pericarp
• Pang Xuequn;Zhang Shaoqi;Duan Xuewu;and Ji Zuoliang
• Acta Horticulturae Sinica. 2001, 28(1): 25-30.
• Abstract ( 2443 ) HTML ( 3052 ) PDF (238KB) ( 3052 ) 　　　
• Anthocyanin in pericarp of litchi fruits (Litchi chinensis Sonn. cv. Huizhi ) was
  isolated to study its color and stability at different pH and temperatures. The color of the anthocyanin was changed after dilution in different pH buffers , its color was red at pH1. 47 , orange at pH 3. 30 , colorless at pH 4. 69 and brown at pH 7. 01. At pH 4. 69 , it could return red when acid was added ,
  but at pH 7. 01 it could not . The absorption value of anthocyanin at 510 nm decreased as the pH rose and at pH 7. 01 the absorption peak shifted from 510 nm to 498 nm , which was consistent with the color of the anthocyanin in different pH solutions. With the pH decrease , the anthocyanin was more
  stable but still showed the usual loss during storage. The color of nthocyanin extract changed into red-brown at pH 1. 47 and 3. 03 , brown at pH 4. 69 , dark brown at pH 7. 01 after storage at room temperature for 20 days. These results showed that the anthocyanin decolored , turned brown and became
  unstable as pH increased. The results also showed that the anthocyanin became stable under low temperature.
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• Effect of Iso-osmotic Ca(NO₃)₂ and NaCl on Growth of Tomato Seedlings
• Liu Zhiyuan;Zhu Zhujun;Qian Yarong;and Yu Jingquan
• Acta Horticulturae Sinica. 2001, 28(1): 31-35.
• Abstract ( 1838 ) HTML ( 1805 ) PDF (201KB) ( 1805 ) 　　　
• The tomato seedlings had different responses to iso-osmotic Ca(NO₃)₂ and NaCl solution. The reduction of plant growth under 28 and 56 mmol/ L Ca(NO₃)₂ solution was greatly smaller than those under iso2osmotic NaCl solution , but under 84 mmol/ L Ca(NO₃)₂ solution the inhibition was the same as that under the iso2osmotic NaCl solution. The plant growth was inhibited by high concentration of Ca(NO₃)₂ mainly through osmotic stress. Ion stress is the main reason of growth inhibition under NaCl : including destroy of membrane structure and reduction of potassium uptake.
  
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• Effects of Different Low Temperature Treatments on Compositions of Polar Lipids in Cucumber Cotyledons
• Yang Ling
• Acta Horticulturae Sinica. 2001, 28(1): 36-40.
• Abstract ( 1371 ) HTML ( 1468 ) PDF (109KB) ( 1468 ) 　　　
• This paper studied the changes of polar lipid and fatty acid compositions in cucumber cotyledons under different temperature (25 ℃, 20 ℃, 15 ℃, 10 ℃) treatments. Though there was no visible injury from chilling , polar lipid compositions in cucumber cotyledons changed. Under 15 ℃, the relative content of DGDG significantly increased ; PC , PE decreased greatly. Molecular
  species of main polar lipids also changed , more unsaturated molecular species in MGDG, DGDGand PC increased. These changes resulted in the increase of unsaturated fatty acids of total lipid , especially linolenic acid. Therefore , the chilling acclimation of cucumber cotyledons could be achieved by pre-exposure to 15 ℃for seven days.
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• Effects of Chilling under LowLight on Photosynthesis and ChlorophyllFluorescence Characteristic in Tomato Leaves
• Hu Wenhai and Yu Jingquan
• Acta Horticulturae Sinica. 2001, 28(1): 41-46.
• Abstract ( 1585 ) HTML ( 3887 ) PDF (235KB) ( 3887 ) 　　　
• Exposure of tomato plants to low temperature (10 ℃ and 5 ℃) under low light (60 μmol·m^-2·s^-1) resulted in an completely inhibition of plant growth and decreased chlorophyll content , net photosynthetic rate (Pn) , stomatal conductance (Gs) and intracellular CO₂ (Ci) concentration except Ci for the lower leaves during chilling at 5 ℃. During the recovery , Pn for 10 ℃treatment arose to a value similar to control , but Pn for 5 ℃treatment did not . Chilling at 10 ℃did not affect Fv/ Fm , but slightly decreasedΦPS Ⅱalthough it recovered soon after chilling stress. However , chilling at 5 ℃ considerably decreased Fv/ Fm and ΦPS Ⅱ, and both recovered slowly after
  chilling stress.
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• The Study on Change of Main Nutrition during Maturation Process inSeed-Pumpkin Seed
• Wang Ping;Zhao Qingyan;Wang Ruojing;and Cui Shimao
• Acta Horticulturae Sinica. 2001, 28(1): 47-51.
• Abstract ( 1718 ) HTML ( 1554 ) PDF (197KB) ( 1554 ) 　　　
• The paper studied change of main nutrition during maturation process of six varieties of seed pumpkin seed. The results showed that : (1) At first the soluble sugar and starch contents increased , then gradually decreased while fat and protein contents increased in seed. (2) The seed-pumpkin seed was of high fat content (51 % - 56 %) and 33 % - 61 % unsaturated fatty acid. Its
  protein contented was 28 % - 36 % , and it contented 8 kinds of essential amino acids , the seed also contained abundant minerals and vitamins.
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• Germplasm Resource and Habitat Types of Wild Begonia in Subtropical Region of Guizhou
• Dai Zhengfu and Zhou Zhengbang
• Acta Horticulturae Sinica. 2001, 28(1): 52-56.
• Abstract ( 1601 ) HTML ( 1217 ) PDF (99KB) ( 1217 ) 　　　
• There are nineteen species of wild begonia including new recorded species in subtropicalregion of Guizhou. They are distributed in Qianbei , Qiandongbei , Qiandongnan and Qiannan respectively. Its habitat types are classified into shrub-wet land , forest-wet land and shade-wet rock type, of which , the most species belong to shade-wetrock type. Ornamental and dual-purpose type are classified according to its utilization , of which , the most species belong to ornamental type. B .crassirostris Irmsch. , B . truncatiloba Irmsch. , B . calophylla Irmsch. , B . summoglabra Yu , and B . parvula Levl . are in imminent danger.
  
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研究报告

• Transformation of Apple Using Super Expression Cowpea Trypsin Inhibitor(CpTI) Gene
• Da Kedong;Cui Decai;Zhang Song;Jing Demin;Wang Bin;and Shu Huairui
• Acta Horticulturae Sinica. 2001, 28(1): 57-58.
• Abstract ( 1418 ) HTML ( 1181 ) PDF (172KB) ( 1181 ) 　　　
• Cowpea trypsin inhibitor ( CpTI) gene in super expression binary vector PECp was transferred to apple cultivar ‘Gala’ using Agrobacterium mediated leaf disc transformation method. GUS staining and southern blot confirmed that CpTI gene was inserted into apple genome.
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• Immuno-gold Localization of Recognition Prorein in the Self-Pollination Style of Shatinyu
• Xue Miaonan;Li Yiping;Zhang Xinhui;and Yang Jihua
• Acta Horticulturae Sinica. 2001, 28(1): 59-61.
• Abstract ( 1503 ) HTML ( 1075 ) PDF (146KB) ( 1075 ) 　　　
• By immuno-gold, the recognition protein in the stylar passage cell of shatinyu (Citrus grandis var. Shatinyu Hort. ) 1- 3 days after self􀀁pollination was examined. The gold􀀁grains were located on the endoplasm reticu􀀁
  lum, golgiosome and cellulose wall successively. The change of the gold􀀁grains distribution in the 1/ 2 stylar passage cell of shatinyu was identical to that of pollen tube stopping glycoprotein on the cellulose wall of 1/ 2 stylar passage cell which was the recognition site of self-incompatibility of shatinyu.
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• Study on Cryopreservation of in Vitro Cultured Cabernet France Shoot Tips
• Zhao Yanhua and Wu Yongjie
• Acta Horticulturae Sinica. 2001, 28(1): 62-64.
• Abstract ( 1244 ) HTML ( 1064 ) PDF (143KB) ( 1064 ) 　　　
• Bud location , dehydration , prefreezing and subculture time were studied on grapevine Cabernet France shoot tip cryopreservation by the encapsulation-dehydration process. Best result (40 %) was obtained in the following
  procedare : 70 select lateral buds after four months’subculture as explants , preculture them for 3 days , dehdrate for five hours then cool them in two steps and finally subculture them on standard culture medium.
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• Roles of Abscisic Acid and Ethylene in the Regulation of Litchi Ripening andColoration
• Yin Jinhua;Gao Feifei;Hu Guibing;and Zhu Shuhua
• Acta Horticulturae Sinica. 2001, 28(1): 65-67.
• Abstract ( 1668 ) HTML ( 1804 ) PDF (91KB) ( 1804 ) 　　　
• Contents of endogenous ABA and ethylene in Litchi fruit during maturation were determined and STS (Ag₂S₂O₃) which inhibits synthesis of ethylene was used to study its effects on fruit maturation and coloration. The results showed that the content of ABA increased rapidly a week before pericarp coloration. The change of ethylene was similar to that of ABA except that there was a small climax of ethylene taking place after ABA increased. With the occurrence of ethylene climax , there was a rapid change in sugar content and invertase activity. STS treatment delayed litchi fruit coloration and maturation. It was suggested that the increase of ABA content might have induced the synthesis of ethylene , which consequently promoted fruit maturation and coloration.
  
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• Construction of Molecular Linkage Map in Grape Using RAPD Markers and F₁ Population of Interspecific Cross
• Luo Sulan;He Puchao;Zhou Peng;and Zheng Xueqin
• Acta Horticulturae Sinica. 2001, 28(1): 68-70.
• Abstract ( 1527 ) HTML ( 1200 ) PDF (150KB) ( 1200 ) 　　　
• A set of 280 randomoligonucleotide perimers was screened for random amplified polymorphic DNAs (RAPD) fragments within a sample of 60 F₁ hybrids to generate RAPD markers and construct the molecular linkage map fromtwo parents 8324296 (V. quinquangularis) and Muscat Rose (V. vinifera) . 89 repeatable RAPD markers as well as four morphological markers (flower type , grape downy mildew2resistance , berry color and juice color) were obtained. The map consisted of 11 linkage groups , it covered a total genetic distance of over 1 033 cM (centimorgan) , the average distance between markers was 17.8 cM. It supplied a linkage framework for a more saturated linkage map of grape.
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• The Simple Estimation of the Breeding Value of Fruit Parents for Hybridization
• Chen Changlan;Jing Shixi;and Jia Jingxian
• Acta Horticulturae Sinica. 2001, 28(1): 71-73.
• Abstract ( 1176 ) HTML ( 1035 ) PDF (146KB) ( 1035 ) 　　　
• By studying the breeding value of compactness character of pears , the simple way to estimate the breeding value of fruit parents in conventional breeding was probed.
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• Establishment of Efficient Shoot Regeneration System of Chinese Cabbage (Brassica campestris ssp. pekinensis) Inbred Line‘AB-81’and Studies of Transient Expression of gus A Gene
• Wang Huoxu;Wang Guanlin;Wang Xiaoyan;Fang Hongjun;Jia Shirong;Tang Yixiong;and Wei Yutang
• Acta Horticulturae Sinica. 2001, 28(1): 74-76.
• Abstract ( 1355 ) HTML ( 1370 ) PDF (88KB) ( 1370 ) 　　　
• An efficient shoot regeneration systemwas established using cotyledon and leave cuts of the inbred line‘AB-81’as explants. On the MS medium supplemented with 10 mg/L AgNO₃ , 0. 2 mg/L TDZ, 2 mg/L NAA and 0.25mg/L ABA , the regeneration frequency of the cotyledon and leave explants was up to 93. 3% and 90% respectively. The mean number and the most number of shoots per explant was 3 - 6 and 21 respectively. Cotyledons of AB281 were inoculated with Agrobacterium tumefaciens strain EHA105 containing pMOG410 carrying gus A-intron gene. The optimum conditions for the transient expression of gus A gene were as follows : preculture time of 2 - 3 days , bacterium density of OD 0. 3 - 0.5 , infection time of 2 - 10 min , and coculture time of 2 - 3 days.
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• Establishment of an Efficient RAPD Protocol in Pepper and Its Application in Genetic Purity Testing of F₁ Seeds
• Huang Sanwen;Zhang Baoxi;Guo Jiazhen;Yang Guimei;Zhu Dewei;DuMeizhen;and Yang Jie
• Acta Horticulturae Sinica. 2001, 28(1): 77-79.
• Abstract ( 1648 ) HTML ( 1125 ) PDF (138KB) ( 1125 ) 　　　
• In this report, a simple and efficient protocol for RAPD assay in pepper was established through optimization in three main procedures DNA extraction, PCR, electrophoresis. The protocol was employed in screening RAPD markers for genetic purity testing of‘Zhongjiao’serial hybrid cultivars. A total of 12 stable and strong RAPDs were identified to distin guish all 9 F₁ s from their parental lines.
  
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• Sonication-assisted Agrobacterium-mediated Transformation of Cucumber
• Yao Chunna and Wand Yafu
• Acta Horticulturae Sinica. 2001, 28(1): 80-82.
• Abstract ( 1765 ) HTML ( 1398 ) PDF (94KB) ( 1398 ) 　　　
• Sonication-assisted Agrobacterium-mediated transformation (SAAT) tremendously improves the efficiency of Agrobacterium infection by introducing large numbers of microwounds into the target plant tissue. Cotyledon and hypocotyl segments of cucumber were transformed by Agrobacterium rhizogenes A4. We evaluated the effects of the duration of sonication treatment , length of explant preculture , addition of acetosyringone (AS) and the orientation of the explants during the cocultivation on cucumber transformation.
  
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• F₁ ‘Cherry Begonia’Series from Interspecif ic Hybridization of Begonia semperflorens ×Begonia tuberhybrida
• Zhang Chengmin;Sun Weibang;Zhang Shibao;Guo Ruixian;Wei Zhaoxiang;and Zheng Ruoxian
• Acta Horticulturae Sinica. 2001, 28(1): 83-85.
• Abstract ( 1395 ) HTML ( 1223 ) PDF (144KB) ( 1223 ) 　　　
• The new F₁ ‘Cherry Begonia’series has been selected from the interspecific hybridization between Begonia semperflorens (maternal plant , pink) and Begonia tuberhybrida (red) . The‘Cherry Begonia’series has highly
  pendent double-petals flowers hanging on the flower-spikes. It not only has characteristics of the stronger adaptability, long florescence and easy to propagate and cultivation as same as the maternal plant , but also it has the bright and bigger flowers , more flowers on each inflorescence and richer flower color range (red, pink and white) in comparison with Begonia semperflorens.
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新品种

• ‘Wancui’—A New Variety of Actinidia deliciosa
• Zhu Liwu;Ding Shilin;Wang Moucai;and Chu Lin
• Acta Horticulturae Sinica. 2001, 28(1): 86-86.
• Abstract ( 2038 ) HTML ( 904 ) PDF (137KB) ( 904 ) 　　　
• The new variety ‘Wancui’ was a natural mutant of Actinidia delicious cv. Hayward. The budding and leaf expansion of ‘Wancui’ were 3- 5 days earlier, beginning of spout growth was 6 days earlier and flower ing was 3
  days earlier than those of its stock plant. Its fruit set was 24% higher and yield was 30% higher than that of Hayward.
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• A New Early Fruit Walnut Variety‘Daifeng’
• Xu Ying;ZhangMeiyong;and An Guoning
• Acta Horticulturae Sinica. 2001, 28(1): 87-87.
• Abstract ( 1215 ) HTML ( 966 ) PDF (143KB) ( 966 ) 　　　
• Early fruit walnut variety of Daifeng showed excellent characters with bigger fruit, high yield, thin shell and good quality, It is highly resistant to the adverse growing conditions. Fruit shape, shell thin and kernel percent
  achieved the international standard of excellent walnut varieties.
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• A New Chinese Cabbage F₁ Hybrid‘Yubaicai No. 12’
• Gao Muqiang;Zhang Xiaowei;Geng Jianfeng;Yuan Yuxiang;Jiang Wusheng;Han Yongping;and Li Genyi
• Acta Horticulturae Sinica. 2001, 28(1): 88-88.
• Abstract ( 1454 ) HTML ( 854 ) PDF (139KB) ( 854 ) 　　　
• ‘Yubaicai No . 12’is a new Chinese cabbage F₁ hybrid, whose parents are self􀀁incompatible double haploid lines induced by isolated microspore cultrue. It takes 80- 85 days from sowing to maturity , its head shape index
  is 1. 5. This variety is resistant to turnip mosaic virus (TuMV) , downy mildew and black spot. The marketable yield is 96. 5- 106 t/ hm². It also has excellent quality and good storage tolerance.
  
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• ‘Jiyan No. 5’—A Sweet Pepper F1 Hybrid Produced by Male Sterile Lines
• Fan Yanqin and Liu Yun
• Acta Horticulturae Sinica. 2001, 28(1): 89-89.
• Abstract ( 1296 ) HTML ( 785 ) PDF (60KB) ( 785 ) 　　　
• ‘Jiyan No. 5’is a F₁ hybrid of sweet pepper by crossing the genic male sterile line AB91 with inbred line JR. The variety has an early maturity and good tolerance to diseases. It is heat tolerant and has high yield and good
  quality. The variety is suitable for protect production and open field production.
  
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• A New Middle Cowpea Variety ‘Yangjiang No. 40’
• Xu Dongjin;Jia Zhiming;Wang Hongzhen;Zhu Duan;Pan Xuechun;Chen Dengjin;and Zhou Yunde
• Acta Horticulturae Sinica. 2001, 28(1): 90-90.
• Abstract ( 1449 ) HTML ( 850 ) PDF (62KB) ( 850 ) 　　　
• ‘Yangjiang No. 40’ is a new middle cowpea variety, which is bred from ‘Zhijiang No . 28-2’ in 1994. It is heat tolerant. Its plant grew vigorously. It yields 22. 5 t/ hm² in spring and 19. 5 t/ hm² in summer in the district of the Yangtze River. The pod is light-green and 60-70 cm length. This variety has high marketable percentage of pod and good quality .
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