Research Papers

• Studies on Relationships Among Endogenous Hormones，Precocious Flower and Easy Formability in Different Scion-Rootstock Combinations for Young Apple Trees in Weibei Area
• ZHANG Baojuan1，ZHANG Dong1，MA Juanjuan1，SONG Chunhui1，LI Wenqiang2，ZHAO Caiping1，YANG Jie1，and HAN Mingyu1,*
• Acta Horticulturae Sinica. 2017, 44(10): 1837-1848. DOI:10.16420/j.issn.0513-353x.2016-0931
• Abstract ( 191 ) HTML ( 681 ) PDF (1386KB) ( 681 ) 　　　
• It is very important for the high efficiency mode of apple production to properly select and combine scion and rootstocks. The easy formability and precocious flower of the young trees are the important indexes to screen the excellent combination of the rootstock，which is closely related to the endogenous hormone content. Three- or four-year young trees from five different scion-rootstock combinations of Fuji apples were used as materials to research the relationship among endogenous hormones，precocious flower and easy formability. The results showed that：vigour rootstock apple tree was tall and vigorous，and the proportion of spurs and the number of inflorescence were significantly smaller than those of the dwarf rootstocks. In addition，the proportion of spurs was high，but the amount of flower was little for M26 combination. The tree of M26 was weak，SH16 combination followed，and T337 finally. It showed that the combination of T337 possessed good growth power and more flower. The content of IAA and ZR in young leaves reached the peak when the spring，autumn shoots began to grow. Furthermore，the content of IAA，ZR，and GA3 in the fine root reached the peak when the root was in the second high growth period. There was a negative correlation between IAA in the fine root and ZR in young leaves，and there was a positive correlation between the above-ground and the underground of IAA and GA3. In the critical period of bud physiological differentiation，IAA and ZR in young leaves were positively correlated with the amount of shoots，ZR and ZR/GA3 were positively correlated with the proportion of spurs，and GA3 was negatively correlated with the number of inflorescence. At the second growth peak of root system，the related indexes of flowering were positively correlated with IAA，ZR，GA3 and IAA/ZR in the fine root. It is suggested that endogenous hormones play an important role in flowering and root growth. Therefore，T337 as a rootstock，reflects the integrity of easy formability and early flower.
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• Water and Mineral Elements Uptake Patterns of‘Thompson Seedless’ Grapevine in Turpan，Xinjiang
• LUO Meng1，WU Guohong2，XU Wenping1,*，LUO Qiangwei2，LOU Yusui1，SUN Feng2，and WANG Shiping1,*
• Acta Horticulturae Sinica. 2017, 44(10): 1849-1860. DOI:10.16420/j.issn.0513-353x.2016-0875
• Abstract ( 239 ) HTML ( 640 ) PDF (814KB) ( 640 ) 　　　
• To investigate the uptake patterns of water and mineral elements at different phenological stages of grapevines in Turpan，four-year-old‘Thompson Seedless’grapevines（Vitis vinifera L.）were cultivated in a closed and recycled nutrition supply system to avoid leakage and evaporation of nutrient solution. The concentrations of Hoagland nutrient solution were set as low level（0.5-fold standard formula concentration），medium level（1-fold standard formula concentration）and high level（1.5-fold standard formula concentration）. During the growth and development of grapevine，the vegetative growth indicators（shoot length，shoot base diameter and leaf area）and reproductive growth indicators（transverse diameter，vertical diameter，single berry weight，yield，soluble solid content and titratable acid content） were measured continuously. The amount of water and mineral elements uptake by the grapevine were calculated according to the decrease of nutrient solution after circulating in a period of time. The concentrations of mineral elements in leaves and berries were measured at different phenological stages. The results showed that in average，4 149.5 m3 ? hm-2 nutritional solution could be uptaken in a year by 1 hm2 grapevine，which contained mineral elements of：N 227.9 kg，P 22.9 kg，K 325.9 kg，Ca 341.8 kg，Mg 117.8 kg，Fe 1.9 kg，Mn 460.6 g，Cu 182.3 g，Zn 198.1 g，B 728.3 g. Forty-seven to seventy-five days after anthesis，the water and mineral element uptake rate of the grapevine reached its maximum. The mineral element content in the leaves and berries presented in a sequence of high concentration treatment > mediun concentration treatment > low concentration treatment.
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• Studies of the Riboflavin Treatment for Promoting the Early Ripening of ‘Kyoho’Grape Berry
• GUO Lili1，XI Feifei2，YU Yihe2，WANG Zhenguang2，ZHANG Guohai2，and GUO Dalong2,*
• Acta Horticulturae Sinica. 2017, 44(10): 1861-1870. DOI:10.16420/j.issn.0513-353x.2017-0386
• Abstract ( 316 ) HTML ( 877 ) PDF (2092KB) ( 877 ) 　　　
• Based on the mechanism of superoxide anion can be produced from the photosensitised riboflavin，three different concentrations（0.1，0.5，1.0 mmol ? L-1）of riboflavin were set to study the effect of exogenous reactive oxygen species（ROS）on‘Kyoho’grape berry development. The results showed that：the riboflavin could promote the development process of grape berry as the exogenous ROS，and the effect of 0.5 mmol ? L-1 treatment was the best，which resulted in sixteen days earlier of the grape berry ripening than the control. Content of soluble sugar and anthocyanin were increased after the treatment of 0.5 mmol ? L-1 riboflavin. At the same time，the hydrogen peroxide（H2O2）content，the pectinase（PG）activity，cellulase activity and lipoxygenase（LOX）activity were all increased at the early development stages of the berry，though the NADPH oxidase（NOX）activity was increased，considering the decreased superoxide dismutase（SOD）activity and production rate of superoxide ion，the increased H2O2 content might generate from other pathway such as LOX. However，the increase H2O2 content did not cause a cell damage（no significant differences of the MDA content）. All in all，the appropriate concentration of riboflavin could promote the grape berry ripening by activating the ROS production pathway.
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• Construction and Preliminary Analysis of Suppression Subtractive cDNA Library of Citrus Infected by Tristeza Virus
• ZENG Zhengrong* and YAN Shutang
• Acta Horticulturae Sinica. 2017, 44(10): 1871-1880. DOI:10.16420/j.issn.0513-353x.2017-0080
• Abstract ( 245 ) HTML ( 623 ) PDF (1140KB) ( 623 ) 　　　

• In order to elucidate the molecular mechanism of the response of citrus to Citrus tristeza virus infection at late stage，a cDNA library was constructed by suppression subtractive hybridization with the RNAs from the leaves of citrus cultivar‘Shiranui’. Two hundred and ten positive clones were picked out randomly from library for sequencing. A total 192 valid ESTs were obtained. Clustering analysis indicated that 77 of them were non-repetitive sequences. Based on GO and KEGG analysis of these ESTs sequences from citrus genome database，the main functions of these ESTs were classified related to disease defense，stress，metabolism，transcription，transport and protein fate. Real-time RT-PCR analysis indicated that expression of 4 genes，including chitinase，jasmonate zim domain 1（JAZ1），calmodulin binding protein，caffeate O-methyltransferase were significantly up-regulated when citrus was infected by Citrus tristeza virus，indicating that these genes may be involved in the resistant response to Citrus tristeza disease. In this paper，we discuss the up-regulation of JAZ1 gene may indicate that the induction of systemic resistance in response to Citrus tristeza disease at late stage of virus infection is inhibited.

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• Cloning and Expression Analysis of CsAP-09：a Transcription Factor Related to Citrus Canker Disease
• JIA Ruirui，HU Anhua，CHEN Shanchun，ZOU Xiuping，PENG Aihong，XU Lanzhen，LEI Tiangang，YAO Lixiao，BAI Xiaojing，HE Yongrui*，and LI Qiang*
• Acta Horticulturae Sinica. 2017, 44(10): 1881-1893. DOI:10.16420/j.issn.0513-353x.2017-0216
• Abstract ( 223 ) HTML ( 933 ) PDF (3802KB) ( 933 ) 　　　
• The AP2 family of Citrus sinensis will be annotated and analyzed while the citrus canker related transcription factor CsAP2-09 will be cloned and analyzed in this study. It is also aimed to confirm the subcellular localization and the expression profiles induced by exogenous hormones，Xanthomonas citri subsp. citri（Xcc）and mechanical wounding. Twelve AP2 genes are extracted and annotated from the public genomic databases of C. sinensis. These 12 AP2s can be divided into 4 categories based on the phylogeny and the motifs. Amino acid sequence and structure analysis indicates that the full-length of CsAP2-09 is 4 159 bp with a 1 467 bp open reading frame which codes a protein containing 488 amino acids. CsAP2-09 contains classic AP2 domains and some specific motifs which give essential and special functions. Subcellular localization results confirm the prediction of the protein localization in nucleus. The promoter contains multiple cis-acting elements involved in plant adversity or hormone responses，such as BOX-W1，CGTCA-motif，TCA-element，WUN-motif and so on. Based on the qPCR data，the CsAP2-09 responds to the exogenous salicylic acid，jasmonic acid methyl ester，mechanical damage and ethylene. Xcc attack can significantly increase the expression of CsAP2-09 in Calamondin but no significant change in Newhall navel orange. All the experiments show CsAP2-09 would be an important transcription factor which is closely associated with the resistance of citrus canker. This gene should be a potential candidate in the molecular breeding to improve the canker resistance of citrus. We have over expressed CsAP2-09 in C. sinensis and obtain 8 transgenic seedlings. Then the evaluation of the resistance will be performed to determine the value of molecular breeding.
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• Effects of Different Environmental Stresses on the Number of Auto- phagosomes and Expression of Autophagy-related Genes in‘Ningyu’Strawberry Leaves
• CHEN Lide，LI Ao，LIU Zhongjie，CUI Mengjie，ZHANG Chaobo，JIA Haifeng，SHANGGUAN Lingfei*，and FANG Jinggui*
• Acta Horticulturae Sinica. 2017, 44(10): 1894-1904. DOI:10.16420/j.issn.0513-353x.2016-0891
• Abstract ( 213 ) HTML ( 817 ) PDF (6921KB) ( 817 ) 　　　
• Autophagy plays an important role in the process of plant responses to external stresses，but limited work has been done so far on autophagy in strawberry. For this purpose，the strawberry plants of Fragaria × ananassa‘Ningyu’were kept under darkness，high salinity，flooding，high temperature and drought to explore the role and activities of autophagy in different stress responses and the semi- quantitative and quantitative expression of 6 autophagy-related genes FaATG5，FaATG6，FaATG7，FaATG8C，FaATG8F，FaATG12 involved in strawberry leaves. The results showed：under the condition of five different stresses，the number of autophagosomes in strawberry leaves were all changed，reaching more than 4，which reached up to a maximum of 35 during 3 days under salt stress，the number of autophagosomes increased initially，afterwards decreased under high temperature and drought stress，indicating that both caused more injury to strawberry leaves. The expression of autophagy-related genes showed an increasing trend under the condition of five different stress environments，under high temperature，the expression levels of FaATG5，FaATG7，FaATG8F and FaATG12 increased firstly and then decreased. Similarly，under drought stress condition，the expression levels of FaATG6，FaATG8C and FaATG8F showed high expression level initially then decreased. FaATG8C and FaATG8F are more important in the formation of autophagosome and response to different stresses in strawberry leaves.
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• Interactions of Flowering Promoting Factor AGL19 with Integrator Factors AGL24 and SOC1 in Brassica oleracea var. italica
• JIANG Wei*，ZHOU Wenwen*，LI Zhaochuang，YAN Kai，WANG Yu，WANG Zhimin，SONG Ming，and TANG Qinglin**
• Acta Horticulturae Sinica. 2017, 44(10): 1905-1913. DOI:10.16420/j.issn.0513-353x.2017-0234
• Abstract ( 241 ) HTML ( 952 ) PDF (3440KB) ( 952 ) 　　　
• In order to clarify the protein interaction mechanisms of flowering promoting factor AGL19 with another two floral integrator factors（AGL24 and SOC1）in flowering pathways，AGL19，AGL24 and SOC1 genes were cloned from Brassica oleracea var. italica，and encoded 221，221 and 214 amino acids，respectively. AGL19，AGL24 and SOC1 were MIKC-type proteins and similar to Brassica oleracea，B. rapa and Chinese cabbage. AGL19 and SOC1 were members of TM3/SOC1 subfamily. Yeast two-hybrid assays showed that the fused strains could grew with blue colonies on QDO/X/A plates，indicating that AGL19 interact with AGL24 and activated the expression of the reporter genes AUR1-C，HIS3，ADE2 and MEL1. However，AGL19 could not interact with SOC1. These results suggested that AGL24 not SOC1 was the direct target protein of AGL19. Additionally，SOC1 also could interact with the AGL24，suggesting that AGL19 can indirectly interact with SOC1 through AGL24.
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• Identification and Analysis of Parthenocarpic Differentially Expressed Sequences in Eggplant
• GUO Yahe，ZHAO Zhen，ZHANG Ying，LIAN Yong，CHEN Yuhui，and LIU Fuzhong*
• Acta Horticulturae Sinica. 2017, 44(10): 1914-1925. DOI:10.16420/j.issn.0513-353x.2017-0374
• Abstract ( 212 ) HTML ( 721 ) PDF (753KB) ( 721 ) 　　　
• The expression patterns of 96 Unique ESTs from the eggplant parthenocarpic SSH-cDNA liabries were identified in the fruit different development stages of both of parthenocarpic and non-parthenocarpic eggplant lines by qPCR method. The results showed that，31 ESTs were significantly different in the relative expression in the eggplant fruits（ovary）development under the condition of low temperature，of which 17 ESTs were up-regulated and 14 ESTs were down-regulated. The analyses of sequences of ESTs indicated that 5 sequences were related to resistance to low temperature，6 sequences were associated with plant hormone metabolism. Some sequences were related to the synthesis of protein and carbohydrate in plant metabolic processes，and 1 EST may be a new gene. Together，some differentially expressed sequences could be used as the candidate genes of the parthenocarpy and low temperature tolerance of eggplant.
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• Screening，Identification，Biocontrol and Growth Promoting Effects of Antagonistic Bacteria WXCDD51 of Tomato Gray Mold Disease and Tomato Leaf Mold Disease
• SHI Yuying1，SONG Haihui1，MIAO Shuang1，GONG Chao2，WANG Hui2，HUANG Haifeng2，CHEN Xiuling1，ZHANG Shumei3,*，and WANG Aoxue1,2,*
• Acta Horticulturae Sinica. 2017, 44(10): 1925-1936. DOI:10.16420/j.issn.0513-353x.2017-0182
• Abstract ( 291 ) HTML ( 557 ) PDF (2067KB) ( 557 ) 　　　
• In this study，a strain，named as WXCDD51，which has strong antagonistic effects on tomato gray mold disease and tomato leaf mold disease，was screened out from the rhizosphere of healthy tomato plants. Based on the morphological，physiological characteristics and 16S rDNA sequence analysis，WXCDD51 was identified as Pseudomonas sp. Mycelial growth assay results showed that the sterile filtrate of WXCDD51 could significantly inhibit mycelial growth of Botrytis cinerea and Fulvia fulva，and the inhibition rates were 94.29% and 94.06% respectively. The pot experiment results showed that WXCDD51 made effective control of tomato gray mold disease and tomato leaf mold disease，the control efficiencies were 66.23% and 69.45%. By dual culture，strain WXCDD51 had different degrees of inhibition on 10 kinds of plant pathogenic fungi and the inhibition rate was about 53.04%–94.59%. The growth-promoting assay showed that WXCDD51 could promote seeds germination and seedling growth of tomatoes. After seeds were treated by the fermentation liquid of WXCDD51（106 cfu ? mL-1），the germination rate of seeds and radicle length increased 80.0% and 62.57% respectively comparing to the control. Under the treatment of the fermentation liquid of WXCDD51（107 cfu ? mL-1），almost all physiological indexes of treated seedlings were significantly higher than that of the control groups. After tomato fruits were soaked in fermented liquid of WXCDD51（107 cfu ? mL-1），tomato fruits decay can be prevented without affecting tomato fruits’ quality.
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• Regulatory Roles of Exogenous IBA in Adventitious Root Formation on the Hypocotyls of Pepper Seedlings
• ZHANG Huanxin，DONG Chunjuan，and SHANG Qingmao*
• Acta Horticulturae Sinica. 2017, 44(10): 1937-1948. DOI:10.16420/j.issn.0513-353x.2017-0156
• Abstract ( 211 ) HTML ( 864 ) PDF (2729KB) ( 864 ) 　　　
• In order to investigate the mechanism of IBA-induced adventitious rooting from hypocotyl cuttings of pepper（Capsicum annuum L.），we detected the process of adventitious rooting，contents of carbohydrates，and expression of genes related to adventitious root formation. The results indicated that exogenous IBA could regulate the adventitious root number in a dose-dependent manner. Compared with the control without IBA，cuttings treated with 150 μmol ? L-1 IBA showed maximum adventitious root number，rooting zone length and average root length. In addition，the expression levels of CaARL1 and CaCycB1 increased greatly after 150 μmol ? L-1 IBA treatment. Adventitious root primordia could occur at both basal and upper segments of hypocotyl cuttings treated with IBA，thus the length of rooting zone was greatly increased. Furthermore，after IBA treatment，the expression levels of CaCWI and CaSTP genes，contents of carbohydrates and activity of cell wall invertase were significantly increased. In conclusion，appropriate concentration of IBA treatment could enhance the accumulation and transformation of carbohydrates at the rooting zone，thereby promoting adventitious root formation on the hypocotyls of pepper seedlings.
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• Cloning and Expression of bHLH Gene from Rosa chinensis‘Slater’s Crimson China’and Its Interaction with Transcription Factors MYB and WD40
• LI Maofu1，YANG Yuan1,2，WANG Hua1，LIU Jiashen1，and JIN Wanmei1,*
• Acta Horticulturae Sinica. 2017, 44(10): 1949-1958. DOI:10.16420/j.issn.0513-353x.2017-0183
• Abstract ( 535 ) HTML ( 1310 ) PDF (5309KB) ( 1310 ) 　　　
• The complete cDNA sequence of RcbHLH gene was identified to reveal the underlying anthocyanin synthesis mechanism by homology-based cloning in the‘Slater’s Crimson China’（Rosa chinensis）. The bioinformatic characteristics，tissue specific expression pattern，and interaction of this gene with MYB and WD40 were investigated. These results showed full length open reading frame of RcbHLH gene was 2 112 bp. It encoded 703 amino acids（Accession number：KY783912）. The RcbHLH had a conserved base domain in its N terminal and a conserved Helix-Loop-Helix domain in its C terminal. The results of analyzing protein system evolution showed that RcbHLH had high homology with the strawberry bHLH and other bHLH protein of plants in Rosaceae. qRT-PCR showed RcbHLH gene was mainly expressed in petal. The results of yeast two-hybrid experiments indicated a MYB-bHLH-WD40 （MBW）complex was formed through the interacting of RcbHLH，PavMYB and PavWD40. These results provided basic information to further illuminate the functions of the RcbHLH gene.
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Research Notes

• Genome-wide Association Mapping of Berry Shape Traits via the Reduced Representation Sequencing in Grape
• ZHANG Heng1,2,*，LIU Zhongjie1,*，FAN Xiucai2，ZHANG Chuan1，CUI Liwen1，LIU Chonghuai2,**，and FANG Jinggui1,**
• Acta Horticulturae Sinica. 2017, 44(10): 1959-1968. DOI:10.16420/j.issn.0513-353x.2016-0845
• Abstract ( 196 ) HTML ( 1146 ) PDF (1476KB) ( 1146 ) 　　　
• Three hundred and four grape germplasms were selected to do the reduced representation sequencing for genome-wide association mapping to detect the genetic loci controlling berry shape. The DNA samples isolated from the grape germplasms were sequenced with a 8.13-fold sequencing depth for each sample，and a total of 466 618 high quality SLAF（specific locus amplified fragment sequencing）labels were acquired，among which 392 374 were polymorphicones. Alignment analysis to the polymorphic SLAF labels identified 4 241 729 SNP，from which 481 192 SNP were obtained with the screening based on a proportion of genotyped efficiency over 0.8 and minor allele frequencies higher than 0.05. In this study，the loci controlling grape berry shape were the first reported，and the devotions of the gene variances for berry shape were higher than 11% except of the SNP rs26137624 based on GLM. Further analysis using GLM and MLM model detected 7 and 3 SNPs，respectively，and all these 10 SNPs located on chromosome 2. Through filtering the associated upstream and downstream areas around each significant SNP，and detecting these related regions for functional genes，10 genes with predicted functional annotations and 7 without named protein product predicted were found.
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• Aged Peach Orchard Soil Inhibit the Growth of Malus hupehensis Seedlings
• LIU Yusong，DUAN Yanan，CHEN Xuesen，SHEN Xiang，YIN Chengmiao*，and MAO Zhiquan*
• Acta Horticulturae Sinica. 2017, 44(10): 1969-1977. DOI:10.16420/j.issn.0513-353x.2017-0021
• Abstract ( 217 ) HTML ( 758 ) PDF (684KB) ( 758 ) 　　　

• A pot experiment was conducted to study effects of aged peach orchard soil on the growth of Malus hupehensis Rehd. seedlings，and M. hupehensis Rehd. was used in this study. The biomass，root structure parameters，root respiration rate，root protection，photosynthetic parameters of M. hupehensis Rehd. and microbial population in soil were measured. Three treatments were designed as follows：fallow soil（the soil beside peach orchard），methyl bromide fumigation aged peach orchard soil and aged peach orchard soil. The results showed that the plant height，plant ground diameter，fresh weight and dry weight of M. hupehensis Rehd. in aged peach orchard soil were significantly lower than those of the fallow soil and methyl bromide fumigation aged peach orchard soil，and 17.59%，13.51%，23.22% and 28.61% were lower than those of the fallow soil；and 20.54%，17.29%，33.28% and 39.21% were less than those of methyl bromide fumigation aged peach orchard soil. The root length，root surface area and root volume were decreased by 37.57%，36.33% and 46.63% respectively compared with fallow soil，and 43.62%，48.67% and 54.11% were lower than those of methyl bromide fumigation aged peach orchard soil. The root respiration rate，superoxide dismutase（SOD），peroxidase（POD）and catalase（CAT）activities were significantly decreased by 28.21%，43.91%，44.46%，51.44% respectively compared with fallow soil，and 36.88%，57.78%，54.88% and 57.59% were lower than those of methyl bromide fumigation aged peach orchard soil respectively. The net photosynthetic rate（Pn）were decreased by 40.8% and 49.65% respectively compared with fallow soil and methyl bromide fumigation aged peach orchard soil. The fungi number of aged peach orchard soil was 1.91 times higher than that of fallow soil，while the bacteria number was 47.80% lower than that of fallow soil. The ratio of bacteria to fungi was：fallow soil > methyl bromide fumigation aged peach orchard soil > aged peach orchard soil. Therefore，M. hupehensis Rehd. seedlings directly planted in aged peach orchard soil was not good for its growth. But after methyl bromide fumigation，the damage effect was obviously reduced.

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• Distribution and Molecular Characterization of Citrus yellow vein clearing virus in Zhejiang Province
• ZHANG Yanhui1，LIU Yingjie1，WANG Qin1，LI Xueyan2，HU Wenzhao3，DAI Xiangsheng4，and ZHOU Yan1,*
• Acta Horticulturae Sinica. 2017, 44(10): 1978-1984. DOI:10.16420/j.issn.0513-353x.2017-0311
• Abstract ( 253 ) HTML ( 712 ) PDF (710KB) ( 712 ) 　　　
• In this study，181 citrus samples collected from 12 districts and counties in Zhejiang Province were detected by reverse transcription-polymerase chain reaction（RT-PCR）. The results showed that Citrus yellow vein clearing virus（CYVCV）was first detected in Bergamot（Citrus bergamia）and Ehime Kashi 38（C. reticulata）from Zhejiang Province with an incidence of 68.0% and 47.2%，respectively. The complete genome sequences of 4 CYVCV isolates from Zhejiang Province were sequenced and characterized. Comparisons of the whole genome sequences of these 4 CYVCV isolates as well as 11 isolates previously reported from around the world revealed the sequence identity ranged from 96.9%–99.8% at nucleotide level and 97.9%–99.4% at amino acid level，respectively，indicating that there is a very low level of sequence heterogeneity among CYVCV isolates of different geographic origins and hosts.Although CYVCV isolates from Zhejiang Province were clustered in the same clade in the phylogenetic tree，other isolates from China did not show a relationship with the geographic origin of the sampled trees.
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• Involvement of Induced Resistance by Methyl Jasmonate to Bacterial Wilt and Reactive Oxygen Species Metabolism in Pepper Seedlings
• XIANG Miaolian1，ZHAO Xianyang1，CHEN Ming1，FU Yongqi1，and ZENG Xiaochun1,2,*
• Acta Horticulturae Sinica. 2017, 44(10): 1985-1992. DOI:10.16420/j.issn.0513-353x.2017-0004
• Abstract ( 364 ) HTML ( 724 ) PDF (706KB) ( 724 ) 　　　
• To explore the relationship between methyl jasmonate（MeJA）-induced resistance to bacterial wilt and enzymes related to reactive oxygen species metabolism in pepper，the susceptible variety‘Yuehong 1’and resistant variety‘Xinxiang 8’were used as the experimental materials in the study. Seedlings were inoculated with Ralstonia solanacearum after 12，24，48，72 and 96 h of spraying treatment with 0.1 mmol ? L-1 MeJA solution，respectively. The indexes of bacterial wilt，the activities of enzymes related to reactive oxygen species（ROS）metabolism including superoxide dismutase（SOD），catalase（CAT），peroxidase（POD）and ascorbate peroxidase（APX），and the contents of malondialdehyde（MDA）were determined. The results showed that the bacterial wilt indexes of‘Yuehong 1’and‘Xinxiang 8’decreased at first and then increased with the times of MeJA treatment increasing from 12 to 96 h，but were always lower than that of the control，while the induced effects were opposite. The optimal treatment time of MeJA-induced resistance to bacterial wilt was 48 h before inoculation. During 0–96 h after inoculation，the SOD，CAT，POD and APX activities of pepper seedlings treated with MeJA were obvious higher than those of the control，and showed a change of increasing gradually and reaching their peaks in 24–48 h after inoculation，and then decreasing，while the MDA contents were always lower than those of the control. These results suggested that MeJA induced resistance to bacterial wilt in pepper seedlings，and the increased activities of enzymes related to reactive oxygen species metabolism and the decreased lipid peroxidation might be involved in this resistance induction.
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• Studies on Genetic Diversity of Cymbidium kanran Populations from the Main Mountains in Jiangxi Province Based on ISSR Marker
• JIANG Yawen，SUN Xiaoqin，LUO Huolin，YANG Boyun*，and XIONG Dongjin*
• Acta Horticulturae Sinica. 2017, 44(10): 1993-2001. DOI:10.16420/j.issn.0513-353x.2016-0889
• Abstract ( 202 ) HTML ( 628 ) PDF (892KB) ( 628 ) 　　　
• This study used ISSR molecular marker to analyze the genetic diversity and genetic structure of 12 Cymbidium kanran populations from the main mountains in Jiangxi Province. The results showed that 123 bands were amplified from 185 individuals from 12 primers，of which 97 bands were polymorphic，the percentage of polymorphic bands was 78.9%. The genetic diversity of 12 populations at species level was higher（PPB = 78.9%，Na = 1.7967，Ne = 1.4461，He = 0.2649，I = 0.3995），while the genetic diversity at population level was lower（PPB = 43.97%，Na = 1.4397，Ne = 1.2478，He = 0.1462，I = 0.2204）. The genetic differentiation coefficient（GST）was 0.4415，gene flow（Nm）was 0.6325，these indicated that the genetic differentiation in the populations was higher than the genetic differentiation among the populations.Based on cluster analysis by UPGMA，12 C. kanran populations were classified into two groups，the first group included nine populations of ZX，WYS，WYSX，CYX，JA，YF，JGS，SW and SWX，the second group included three populations of SC，AY and CY，the result of cluster analysis showed no obvious correlation with the distribution of C. kanran populations according to the mountains. Mantel test showed that there was no significant correlation between genetic distance and geographical distance（r = 0.3791）.
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• Prokaryotic Expression of Basella rugose mosaic virus CP Gene and Preparation of Its Polyclonal Antiserum
• LI Xiaoqin1，ZHANG Pengyuan1,2，REN Longhui1,3，DAI Jinran1，WANG Jianguang1,*，and CHEN Suiyun1,*
• Acta Horticulturae Sinica. 2017, 44(10): 2001-2007. DOI:10.16420/j.issn.0513-353x.2016-0826
• Abstract ( 183 ) HTML ( 563 ) PDF (1589KB) ( 563 ) 　　　
• The full length cp gene of Basella rugose mosaic virus（BaRMV）was obtained by RT-PCR in the infected Mirabilis jalapa L. leaves and the prokaryotic experssion vector of BaRMV cp gene was successfully constructed also. The pET30a-BaRMV-cp vector was transferred into Rosetta(DE3)PlysS. SDS-PAGE results indicated that the His-CP fusion protein was about 40.0 kD in size，which was in accord with the prediction. Target proteins were isolated by cutting the gel slices that contained the 40.0 kD bands which were stained by 0.25 mol ? L-1 KCl. Purified fusion protein was used to immunize New Zealand white rabbits to produce the antiserum. The polyclonal antiserum was detected with indirect enzyme-linked immunosorbent assay（ID-ELISA），the titer of the antiserum was1︰16 000，Western-blotting analysis showed that the antiserum has very strong specificity. Detected the field susceptible M. jalapa L. and the laboratory inoculated and infectious Nicotiana benthamiana L. by the antiserum，the test result also showed that the prepared antiserum has good specificity and can be used for large-scale sample testing.
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Review

• Advances in Genes Information Involved in Pectin Metabolism in Fruit
• CHEN Kaili，XU Ke，ZHANG Xiancong，WANG Yanan，WANG Zhihui，and WANG Xun*
• Acta Horticulturae Sinica. 2017, 44(10): 2008-2014. DOI:10.16420/j.issn.0513-353x.2016-0846
• Abstract ( 431 ) HTML ( 1757 ) PDF (659KB) ( 1757 ) 　　　

• CHEN Kaili，XU Ke，ZHANG Xiancong，WANG Yanan，WANG Zhihui，and WANG Xun*

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• Review of Selective Autophagy in Plant Cell
• YANG Xiaolong，LI Yangyang，LIU Yufeng，QI Mingfang，and LI Tianlai*
• Acta Horticulturae Sinica. 2017, 44(10): 2015-2028. DOI:10.16420/j.issn.0513-353x.2017-0144
• Abstract ( 436 ) HTML ( 986 ) PDF (735KB) ( 986 ) 　　　

• Plants continuously produce damaged organelles and proteins in the process of growth and response to environmental stresses，which must be timely and appropriately degraded. Autophagy is a major cellular degradation pathway conserved during evolution in plants. Autophagosomes selectively recognize and target specific compartments and protein complexes through cargo receptor proteins，then deliver them to the vacuole for degradation and recycling，which regulates the critical reactions within the cell. Researches in recent years have revealed the importance of autophagy in many aspects of plant life. In this article，the latest advances of plant cell selective autophagy are reviewed，including autophagosome formation，cargo recognition and types of selective autophagy，and the role in growth and development，immune，and response to abiotic stress.

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New Cultivars

• A New Cultivar of Actinidia arguta‘Xinlü’
• QIN Hongyan，FAN Shutian，AI Jun*，YANG Yiming，WANG Zhenxing，LIU Yingxue，ZHAO Ying，XU Peilei，ZHANG Baoxiang，LI Changyu，and ZHANG Qingtian
• Acta Horticulturae Sinica. 2017, 44(10): 2029-2030. DOI:10.16420/j.issn.0513-353x.2017-0161
• Abstract ( 314 ) HTML ( 512 ) PDF (884KB) ( 512 ) 　　　
• ‘Xinlü’is a new cultivar of Actinidia arguta Planch. with strong cold resistance and stora- bility，which was selected from wild kiwifruit groups. The fruit is obovate with green and smooth peel，the average fruit weight is 12.4 g and the maximum fruit weight is 17.0 g，fruit shape index is 1.22. The flesh is green and tender，which is rich in juice and taste pleasant sweetness and sourness. Soluble solids content is 15.7%，soluble sugar content is 7.9%，total acid content is 1.2% and vitamin C content is 465 mg ? kg-1. The fruit quality is excellent. It can be planted in the open field and the fruit ripen in the early September in Jilin. This cultivar can bear fruits after 3–4 years planted，which has high yield of 9 832.8 kg ? hm-2.
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• A Heat-tolerance Cucumber Hybrid‘Zhongnong 38’
• GU Xingfang*，ZHANG Shengping，WANG Ye，and MIAO Han
• Acta Horticulturae Sinica. 2017, 44(10): 2031-2032. DOI:10.16420/j.issn.0513-353x.2017-0608
• Abstract ( 311 ) HTML ( 564 ) PDF (861KB) ( 564 ) 　　　
• ‘Zhongnong 38’is a new cucumber F1 hybrid，which was developed by crossing 081048 as female parent with 02465 as male parent. It grows vigorously with mild branches，and has a strong sustain fruit setting ability. It’s fruit is about 35 cm in length with short neck and dark-green color. It is highly resistant to CGMMV and WMV，resistant to powdery mildew，downy mildew，and other diseases. Also it is tolerate to high temperature. It is suitable for open field cultivation.
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• A New Cucumber Hybrid‘Lifeng’
• LIN Yu’e*，LIANG Zhaojun，WANG Rui，HE Xiaoming，WU Tingquan，HUANG Hexun，and JIANG Biao
• Acta Horticulturae Sinica. 2017, 44(10): 2033-2034. DOI:10.16420/j.issn.0513-353x.2017-0159
• Abstract ( 361 ) HTML ( 370 ) PDF (977KB) ( 370 ) 　　　
• ‘Lifeng’is a new cucumber hybrid of South China type，which was crossed by female parent‘Guannong-863’with heat tolerance and male parent‘Jieyang Wupiqing-311’with disease resistance. This hybrid grows vigorously with early maturity and high yield. Its fruit is cylindrical and about 25 cm in length. The single fruit weight is about 400 g with excellent quality，good taste. The fruit is tolerant to long storage and transportation. This hybrid is resistant to high temperature and Fusarium wilt，the yield of which can reach up to 60.0 t ? hm-2. It is suitable for planting in spring and autumn in South China and Southeast Asia.
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• A New Potherb Mustard Cultivar‘Yongxue 4’
• REN Xiliang*，WANG Yuhong，MENG Qiufeng，and HUANG Yunping*
• Acta Horticulturae Sinica. 2017, 44(10): 2035-2036. DOI:10.16420/j.issn.0513-353x.2017-0078
• Abstract ( 385 ) HTML ( 521 ) PDF (4301KB) ( 521 ) 　　　
• ‘Yongxue 4’is a new hybrid potherb mustard. The cultivar is characterized by strong growth，light-green and double serrate leaf，whose blade margin is shallow sawtooth，glossy and no wax on the leaves. There are 27 effective tillers on average. The fresh weight is 1.4 kg per plant and its total yield is about 75 t ? hm-2. Its bolting tolerance is medium. It is well resistant to virus disease. The growth period is 105 days from sowing to harvest.‘Yongxue 4’owns fine quality of processing. The cultivar suits to be planted in the Yangtze River valley.
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• A New Potherb Mustard Cultivar‘Yongxue 4’
• HUANG Qingjun1,*，WANG Hongwei1，and BIAN Tian2
• Acta Horticulturae Sinica. 2017, 44(10): 2037-2038. DOI:10.16420/j.issn.0513-353x.2016-0022
• Abstract ( 369 ) HTML ( 574 ) PDF (1396KB) ( 574 ) 　　　

• REN Xiliang*，WANG Yuhong，MENG Qiufeng，and HUANG Yunping*

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