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    Flower Development and Nectar Secretion of Ziziphus jujuba‘Dongzao’ Under Facility Cultivation
    XIE Wenjie, CHEN Xiling, SHI Guozhao, ZHANG Peng, LI Xingang, ZHANG Rui, DUAN Xiaoshan
    Acta Horticulturae Sinica    2024, 51 (12): 2735-2742.   DOI: 10.16420/j.issn.0513-353x.2024-0070
    Abstract1888)   HTML144)    PDF (1716KB)(635)      

    In this study,by using Ziziphus jujuba‘Dongzao’as a model,the phenological phases of flower development were documented,the process of flower development and nectar secretion was tracked,and the quantity and sugar content of the nectar were assessed under the facility cultivation. The results showed that compared to the open field cultivation,facility cultivation significantly advanced the sprouting stage of jujube floral bud,extended the duration of individual flower blooming and peak flowering periods,and increased both the quantity and sugar content of nectar. Overall,under the facility cultivation,‘Dongzao’exhibited superior in phenological phases,flower development,as well as nectar composition and quantity,compared to those under the traditional open field cultivation.

    Differences in Occurrence of Fruit Sunburn Between Satsuma Mandarin and Ponkan Tangerine and Possible Mechanisms
    WU Zhuoqun, CHEN Pengxu, CHEN Siyi, ZHANG Fuqiong, FANG Hong, ZHU Jian, XIE Zongzhou, LI Chunlong, LIU Jihong
    Acta Horticulturae Sinica    2024, 51 (12): 2743-2757.   DOI: 10.16420/j.issn.0513-353x.2023-0513
    Abstract1210)   HTML57)    PDF (4516KB)(318)      

    Sunburn is a common physiological disorder in citrus production,but the underlying mechanism remains largely unknown. The study was carried out at Fenghuang Mountain,Banyue Town,Dangyang City,Hubei Province in 2021. Satsuma mandarin(Citrus unshiu Marc.)and Ponkan(C. reticulata)were used as experimental materials to investigate the difference in sunburn and potential mechanisms by examining peel injury degree,performing cytological observation,measuring antioxidant content and gene expression levels. The results showed that Satsuma mandarin was more susceptible to sunburn than Ponkan. Significant difference in the incidence rates of sunburn was observed between the two varieties,43.12% for Satsuma mandarin and 22.52% for Ponkan. Sunburn seriously influenced the compactness of pericarp cells and resulted in the accumulation of lignin. Satsuma mandarin fruits were found to accumulate greater level of reactive oxygen species(ROS). In addition,nobiletin,a critical metabolite in the polymethoxyflavone metabolic pathway,was significantly enriched in Ponkan fruit peel. Consistently,transcript levels of CrCHS2CrCHI and CrFNS,encoding crucial enzymes involved in biosynthesis of flavonoid,and CrOMT2 in the polymethoxyflavone pathway were significantly induced in the sunburnned Ponkan fruit peel. Taken together,these findings indicate that the main reason for the difference in sunburn between Satsuma mandarin and Ponkan is that the latter contains more antioxidant that can effectively decrease the ROS accumulation,thus leading to mitigation of sunburn-derived damage.

    Construction of a High-Efficiency Genetic Transformation System in Pepper Leveraging RUBY and CaREF1
    WANG Zhongyi, LIU Yi, HU Bowen, ZHU Fan, LIU Feng, YANG Sha, XIONG Cheng, OU Lijun, DAI Xiongze, ZOU Xuexiao
    Acta Horticulturae Sinica    2025, 52 (4): 1093-1104.   DOI: 10.16420/j.issn.0513-353x.2025-0368
    Abstract1138)   HTML72)    PDF (5906KB)(601)      

    Using the novel visual reporter system RUBY as a screening marker,combined with the regeneration-promoting peptide CaREF1,a pepper genetic transformation system were constructed and identified three efficient recipient materials for gene delivery. The transient expression efficiency of RUBY was significantly higher in the pepper genotypes‘L27’(line pepper),‘Zunla-1’(chili pepper),and‘1-51’(bead pepper)compared to other materials. Agrobacterium rhizogenes K599,carrying the pKSE401-RUBY vector,was used for transformation. After 60 days of culturing on the adventitious bud regeneration medium(MS + 5 mg · L-1 6-BA + 1.2 mg · L-1 IAA + 10 nmol · L-1 CaREF1)and shoot proliferation medium[MS + (5-10) mg · L-1 6-BA + 0.5 mg · L-1 IAA + 3 mg · L-1 GA3 + 10 nmol · L-1 CaREF1],the average regeneration efficiency of cotyledon explants was 84% ± 7%,with 5.52 regenerated shoots per explant and a positive transformation rate about 5‰. This system enabled the first Agrobacterium-mediated visual genetic transformation in pepper and facilitated CRISPR/Cas9-mediated gene editing in multiple pepper germplasms,with editing efficiency 100% in T0 generation plants.

    Research Advances in the Regulation of Plant Hormones in Citrus Fruit Maturation
    JIANG Bo, LÜ Yuanda, LIU Shumei, YAN Huaxue
    Acta Horticulturae Sinica    2024, 51 (12): 2928-2944.   DOI: 10.16420/j.issn.0513-353x.2024-0204
    Abstract889)   HTML45)    PDF (556KB)(12620)      

    Citrus is usually considered as a typical non-climacteric fruit,with abscisic acid being primarily associated with citrus fruit maturity. However,other hormones,such as ethylene,gibberellin,auxin,also contribute significantly to the maturity and color transition of citrus. In production,a variety of natural hormones and synthetic plant growth regulators are commonly utilized to extend the marketing period of citrus fruits and improve postharvest preservation. This paper provides a comprehensive review of current research advances concerning the role of plant hormones on the maturation of citrus fruits. It delves into the complex relationships between these hormones and investigates the regulatory mechanisms controlled by related transcription factors. The primary aim is to provide reference for further research.

    Function Analysis of SlWRKY46 in Regulating Tomato Response to Low Temperature Stress
    SUN Huiru, DANG Fengfeng, REN Min, ZHANG Jianing, FAN Bei, CHEN Guoliang, CHENG Guoting, WANG Yanfeng
    Acta Horticulturae Sinica    2024, 51 (12): 2758-2774.   DOI: 10.16420/j.issn.0513-353x.2024-0014
    Abstract862)   HTML84)    PDF (6426KB)(519)      

    In order to explore the function of SlWRKY46 in response to low temperature stress,the CDS sequence of SlWRKY46 cloned from tomato‘Micro-Tom’leaves was analyzed. The result showed that SlWRKY46 belonged to Ⅱa group of WRKY family and encoded 253 amino acids. SlWRKY46 was localized in the nucleus by subcellular localization analysis. SlWRKY46 had no transcriptional activation activity in yeast. SlWRKY46 could form homologous dimers by yeast two-hybrid yeast(Y2H). The results of real-time fluorescence quantitative PCR(qRT-PCR)showed that the expressions of SlWRKY46 were significantly induced by low temperature and abscisic acid(ABA). The GUS staining of SlWRKY46 promoter transgenic tomato also cloud be induced by low temperature and ABA. The overexpressed SlWRKY46 tomato lines had higher cold tolerance compared with wild-type. The overexpressed SlWRKY46 tomato lines exhibited lower reactive oxygen species accumulation and malondialdehyde levels,and higher antioxidant enzyme activities and proline accumulation compared with wild-type under low temperature treatment. The expression levels of genes related to antioxidant enzyme and ABA showed significant differences between overexpressed SlWRKY46 tomato lines and wild-type under low temperature treatment. Taken together,these results indicated that SlWRKY46 positively regulated the cold tolerance of tomato by antioxidant enzyme and ABA pathways.

    Advances in Plant Promoter Research
    ZHANG Zhiyuan, WANG Dan, XI Zhumei, WANG Xianhang
    Acta Horticulturae Sinica    2025, 52 (4): 1065-1092.   DOI: 10.16420/j.issn.0513-353x.2024-0408
    Abstract811)   HTML28)    PDF (2578KB)(213)      

    In this paper, the structural characteristics of different types of promoters and their roles in gene function research,metabolic engineering,and gene editing(CRISPR/Cas9)were discusses,and summarized the content and methods for promoter identification,aiming to provide insights for a deeper understanding and investigation of the structural characteristics and regulatory mechanisms of plant promoters.

    Phenotypic Diversity Analysis and Ornamental Comprehensive Evaluation of Ninety-six Tree Peony Cultivars Based on Landscape Application
    ZHI Hui, YANG Jingjing, LUO Jianrang
    Acta Horticulturae Sinica    2025, 52 (3): 635-645.   DOI: 10.16420/j.issn.0513-353x.2024-0412
    Abstract771)   HTML14)    PDF (1913KB)(290)      

    In this study,ninety-six tree peony cultivars planted in Northwest A & F University were selected as the research objects. Phenotypic diversity analysis,correlation analysis,cluster analysis and principal component analysis were used to analyze the phenotypic diversity analysis and ornamental comprehensive evaluation of tree peony growth,new branch length and flowering time. The results showed that the diversity index of 15 phenotypic traits ranged from 0.56 to 2.03,and the coefficient of variation ranged from 11.84% to 53.82%. The phenotypic diversity of flower diameter was the highest,and the coefficient of variation of flower number per plant was the highest. Plant height,growth,flower diameter,new branch thickness and population flowering time were significantly correlated with other traits;Ninety-six tree peony varieties were divided into three groups by cluster analysis,and different groups corresponded to different types of garden applications. Principal component analysis simplified phenotypic trait factors into five principal components,with a cumulative contribution rate of 64.69%. New branch thickness,plant height,population flowering time and number of flowers per plant were the main factors of phenotypic variation of tree peony. The ninety-six tree peony varieties were sorted by comprehensive scores,and the top 10 cultivars had scores ranging from 0.66 to 1.18,and the cultivars with excellent comprehensive traits such as‘Yubanbai’‘Shima Nishiki’‘High Noon’‘Okan’and‘Duohua Luohan’were selected according to the comprehensive scores.

    Identification and Expression Analysis of Trihelix Gene Family in Common Bean
    LUO Xinrui, ZHANG Xiaoxu, WANG Yuping, WANG Zhi, MA Yuanyuan, ZHOU Bingyue
    Acta Horticulturae Sinica    2024, 51 (12): 2775-2790.   DOI: 10.16420/j.issn.0513-353x.2023-0972
    Abstract741)   HTML58)    PDF (6349KB)(209)      

    In this study,bioinformatics analysis of the common bean Trihelix gene family were performed,and the expression of the PvTrihelixes under adversity stress were examined using transcriptomic data(RNA-seq)and fluorescence quantitative PCR(qRT-PCR). The results showed that 40 PvTrihelixes genes were identified in common bean genome,which unevenly distributed on 11 chromosomes. Phylogenetic analyses classified PvTrihelixes into five subfamilies(GT-1,GT-2,GTγ,SIP1 and SH4). Five subfamilies were essentially similar in terms of gene structure and conserved motifs. Covariance analysis revealed there are 5 pairs of tandem repeat and 9 pairs of fragment repeat in PvTrihelixes. The promoter analysis revealed that PvTrihelixes contain cis-acting elements related to growth and development,light,hormones,and stress response. Tissue expression analysis revealed the presence of tissue expression specificity in PvTrihelix,and the genes with higher expression levels in flowers all belonged to members of the GT-2 subfamily and the SIP1 subfamily. The qRT-PCR results showed that PvTrihelix6/7/10/11 were significantly up-regulated under drought(6% PEG),PvTrihelix1/21/25/26/31/35 were up-regulated in response to low temperature(4 ℃),and PvTrihelix21/23/25/27/28/40were significantly up-regulated under salt(100 mmol · L-1 NaCl)stress.

    Physiological Changes Associated with Early Watercore in‘Orin’Apples
    SHI Xingxiu, FENG Beibei, YAN Peng, GENG Wenjuan, Jumazira Sharshanmukhan
    Acta Horticulturae Sinica    2025, 52 (1): 171-184.   DOI: 10.16420/j.issn.0513-353x.2023-0891
    Abstract708)   HTML9)    PDF (1346KB)(83)      

    To investigate changes in cell membrane integrity and the content and distribution of intracellular sugar fractions,reactive oxygen species metabolism and osmoregulatory substance content during the development of early watercore disease in‘Orin’apple fruits,and to analyse the changes in the expression of sorbitol transporter metabolism genes MdSDH and MdSOT. The results showed that the incidence of early watercore increased with fruit growth and higher ambient temperatures,and that early watercore may be associated with high temperatures. After the occurrence of watercore,the sorbitol content of vesicles,cytoplasm and intercellular space was higher than that of fructose and glucose;the permeability of vesicle membrane was significantly correlated with the sorbitol content of each organelle,the sorbitol content was always higher,the fructose content was decreased significantly;the permeability of vesicle membrane and cell membrane to different sugars was different;the content of superoxide anion and hydrogen peroxide was significantly elevated compared with that of the watercore-free tissue(4.65% and 20.83% higher),the activities of antioxidant enzymes such as POD,SOD and CAT were significantly reduced,MDA content and relative conductivity were significantly increased,and the level of membrane peroxidation was serious,but the vacuole membrane and cell membrane still maintained a certain degree of permeability;the expression of MdSOT4 was significantly increased,and it was speculated that it might be an important regulator of sorbitol transport as well as metabolism in the tissue of the watercore. Early watercore symptoms can disappear with lower temperatures in October,further suggesting that high temperatures are associated with watery heart occurrence.

    Review and Prospects of Chinese Cabbage Breeding for the Past 70 Years in China
    ZHANG Fenglan, SU Tongbing, ZHANG Bin, YU Shuancang, YU Yangjun, ZHANG Deshuang, ZHAO Xiuyun, WANG Weihong, LI Peirong, XIN Xiaoyun
    Acta Horticulturae Sinica    2025, 52 (5): 1111-1135.   DOI: 10.16420/j.issn.0513-353x.2025-0048
    Abstract694)   HTML88)    PDF (1126KB)(566)      

    This paper systematically summarizes and presents the significant achievements and research advancements in Chinese cabbage breeding over the past 70 years. It focuses on four major phases of Chinese cabbage breeding in China,the innovation and utilization of Chinese cabbage germplasm resources,innovations and breakthroughs in breeding technologies,and the innovation and promotion of Chinese cabbage varieties. Additionally,the paper analyzes several existing challenges in the development of Chinese cabbage breeding and the seed industry in China. These challenges include the lack of large-scale application of efficient biobreeding technologies,insufficient systematic and in-depth exploration and evaluation of superior germplasm resources,severe homogeneity of varieties,substantial gaps between certain cropping types and foreign varieties,the need for further enhancement of research and development capabilities in seed enterprises,the preliminary formation of vegetable seed production base layout,steady improvement in seed quality,and the necessity for further enhancements in seed processing and treatment technologies. The paper proposes future directions for the Chinese cabbage seed industry,focusing on efficient breeding technologies,breeding objectives,seed production,and the improvement of seed quality.

    Effects of Potassium Fulvic Acid on Photosynthetic Characteristics and Fruit Quality of‘Cabernet Gernischt’Grape
    YANG Jiangshan, CHEN Yajuan, DAI Zibo, LI Dou, SHAO Zhang, JIN Xin, WANG Yuhang, WANG Chunheng
    Acta Horticulturae Sinica    2024, 51 (12): 2843-2856.   DOI: 10.16420/j.issn.0513-353x.2024-0036
    Abstract687)   HTML22)    PDF (3721KB)(178)      

    In order to investigate the effects of fulvic acid potassium(FA-K)on photosynthesis of grape leaves and fruit quality. In this experiment,the wine grape‘Cabernet Gernischt’was used as the test material,and different concentrations(30,60,90,120 mg · L-1)of FA-K were sprayed on the leaves at flowering stage,fruit setting stage,expansion stage and color turning stage,and spraying water was used as the control. The photosynthetic pigment content,photosynthetic parameters,chlorophyll fluorescence parameters and fruit quality were measured under different treatments. The results showed that FA-K treatment significantly increased the content of chlorophyll a,chlorophyll b,total chlorophyll and carotene in leaves of‘Cabernet Gernischt’at different growth stages,enhanced PnTrGsFv/FmY(Ⅱ),qPqN and ETR,and reduced Ci and Y(NPQ). The effect of 90 mg · L-1 FA-K treatment was the most significant. At the same time,FA-K also had a certain effect on improving fruit quality. In particular,90 mg · L-1 treatment significantly promoted the accumulation of total soluble sugar,soluble solids,tannins,total phenols,flavonoids and anthocyanins in fruit,which increased by 25.96%,13.81%,27.73%,63.89%,9.48% and 78.57%,respectively,compared with the control,while the relative content of titratable acid was only 0.54%,which was 26.03% lower than that of the control. Principal component analysis results showed that the effects of different concentrations of FA-K on the photosynthetic and fruit quality of‘Cabernet Gernischt’grape leaves were as follows:90 mg · L-1 treatment > 60 mg · L-1 treatment > 120 mg · L-1 treatment > 30 mg · L-1 treatment > the control. In summary,FA-K treatment can significantly enhance the photosynthesis of grape leaves and improve fruit quality,and the effect of 90 mg · L-1 is the most significant.

    Transcriptome Analysis of the Response of Tree Peony‘Hu Hong’Under High Temperature Stress
    BU Wenxuan, YAO Yiping, HUANG Yu, YANG Xingyu, LUO Xiaoning, ZHANG Minhuan, LEI Weiqun, WANG Zheng, TIAN Jianing, CHEN Lujie, QIN Liping
    Acta Horticulturae Sinica    2024, 51 (12): 2800-2816.   DOI: 10.16420/j.issn.0513-353x.2023-0941
    Abstract648)   HTML43)    PDF (5183KB)(231)      

    To analyze the molecular regulation mechanism of tree peony(Paeonia suffruticosa)in southern China,‘Hu Hong’was selected as the test material,the leaves treated at 20 ℃ and 40 ℃ were collected after 0,2,6,12 and 24 h,respectively. Transcriptomics and physiological and biochemical indicators were used to explore the differences in gene expression at different treatment times under high temperature stress. The result showed that the leaves showed water loss and biofilm damage,the relative conductivity of leaves and the proline content increased significantly with the prolongation of high temperature stress time. Free proline,relative conductivity and soluble protein were identified as important indicators to measure the changes of physiological mechanism in the response of peony to high temperature stress. A total of 492 693 unigene and 840 509 transcripts were generated by RNA-seq. 36 174 differentially expressed genes(DEGs)were annotated in 5 databases including GO and KEGG. The GO analysis showed that the main enrichment pathways were the reaction pathway to heat and the cycle pathway of reduced pentose phosphate. KEGG enrichment analysis showed that DEG is involved in signal transduction,photosynthesis and metabolism play a major role in responding to high temperature stress. Among the DEGs,multiple candidate genes and transcription factors involved in heat stress have been screened out,such as heat shock transcription factors PSHSFA2b,PSHSFA3,PSHSFB2a,PSHSFB2b,PSHSFB4,PSHSFC1 and some NAC,MYB,bHLH family genes. The expression of 11 selected genes under high temperature stress was detected by qRT-PCR,and the results were similar to RNA seq.

    Grapevine VvARF18 Regulates Fruit Expansion and Screening of its Interacting Proteins
    LIANG Jing, ZENG Baozhen, LIANG Guoping, MAO Juan, CHEN Baihong
    Acta Horticulturae Sinica    2025, 52 (4): 821-834.   DOI: 10.16420/j.issn.0513-353x.2024-0594
    Abstract634)   HTML56)    PDF (3316KB)(294)      

    In order to investigate the molecular mechanism of grape VvARF18 in fruit development,‘Red Globe’grapes(Vitis vinifera‘Red Globe’)were used as materials,the VvARF18 gene was cloned,and analyzed for its bioinformatics,and the gene was heterologously overexpressed in tomato to verify its effect on fruit development. The CDS of the VvARF18 gene was 22 109 bp in length,coding for 703 amino acids,with a pI of 6.50. Phylogenetic analysis showed that VvARF18 was the closest related to the VrARF18 protein of Vitis riparis;the gene was heterologously overexpressed in tomato. Expression,the longitudinal and transverse diameters of T3 generation transgenic tomato fruits were significantly higher than those of the wild type,and its volume and single fruit mass were significantly increased,suggesting that VvARF18 has a positive regulatory effect on fruit expansion;transcriptional activation assay found that VvARF18 was not toxic but had self-activating activity,and screening of proteins interacting with its conserved functional structural domain B3-Auxin revealed a potential interplay relationship with the signaling complex VvCOP9.

    Transcriptome Screening and Functional Analysis of Dwarf Related Genes in Pear
    LIN Ruxue, WANG Fei, ZHANG Yanjie, MA Li, LIU Xiaofeng, LI Shuran, LIU Yalong, WANG Chen, JIANG Shuling, OU Chunqing
    Acta Horticulturae Sinica    2025, 52 (3): 545-560.   DOI: 10.16420/j.issn.0513-353x.2024-0481
    Abstract628)   HTML57)    PDF (1622KB)(297)      

    To screen the dwarf trait related genes,the shoot length and internode length of 12 pear varieties or selections with different plant architectures were measured during the new shoot growth period. The genes differentially expressed in the growth points,leaves and phloem among three pear varieties ‘Zaosu’(vigorous),‘Jinxiang’(semi-dwarf)and‘Zhongai 1’(dwarf)at different periods were screened by transcripome sequencing analysis. Combined with the expression verification in new shoot phloem of 12 varieties or selections,dwarf trait related candidate genes were obtained. One of the candidate genes PcGA2ox2 was overexpressed in tobacco to analyze its function. The results showed that the new shoot length of‘Zaosu’,‘Jinxiang’and‘Zhongai 1’had a slight difference in the early growth period,while showed a significant difference in the middle and late growth period of new shoot. The internode length of dwarf varieties were shorter than that of vigorous varieties in all test periods. The shoot length and internode length of six vigorous varieties were significantly longer than those of six dwarf or semi-dwarf varieties or selections in the middle and late growth period of new shoot. The number of differentially expressed genes between two samples ranged from 1 882 to 5 729 in different tissues,and periods of three varieties. Among them,51 differentially expressed genes showed an increase or decrease in expression levels with the decrease of new shoot length in both leaf and phloem. Nine differentially expressed genes in new shoot phloem of three varieties were selected to verify the expression levels by RT-qPCR in the phloem of 12 pear varieties or selections in the late growth period of new shoot. Among them,four genes showed a tendency of significant higher or lower expression in dwarf varieties or selections than that in vigorous varieties or selections,and were identified as candidate genes related to dwarf trait. One of the dwarf candidate genes was a member of GA2ox family genes. Total 15 members of the GA2ox gene family were further identified in the‘Zhongai 1’genome,and the expression of the candidate PcGA2ox2 gene was significantly higher in new shoot phloem of dwarf varieties or selections than that of vigorous varieties. Then PcGA2ox2 gene was overexpressed in tobacco,the transformed plants were significantly smaller than the control plants. In conclusion,PcGA2ox2 gene plays a key role in the dwarf trait of‘Zhongai 1’pear.

    Diversity Analysis of Phenotypic Traits for Chinese Herbaceous Peony Cultivar Resources
    QIAN Jingyang, LU Lili, LI Qin, TANG Yuhan, TAO Jun, ZHAO Daqiu
    Acta Horticulturae Sinica    2025, 52 (2): 349-364.   DOI: 10.16420/j.issn.0513-353x.2024-0277
    Abstract622)   HTML30)    PDF (4389KB)(183)      

    Genetic variation analysis,correlation analysis,cluster analysis and principal component analysis were used to analyze the diversity of 48 phenotypic traits in 167 Chinese herbaceous peony cultivars. The results showed that both qualitative and quantitative traits of Chinese herbaceous peony cultivar resources had high levels of phenotypic diversity,with quantitative traits being more diverse than qualitative traits. Traits such as compound leaf type,flower color,stamen petal shape and color, gynoecium stigma color,scale bud differentiation ability,and flower branch length are important reference indices for measuring the level of phenotypic diversity in herbaceous peony cultivars. R-type cluster analysis classified quantitative traits into 4 categories,mainly reflecting growth potential and leaf traits,floral traits,flowering cycle traits,and phenological period traits. Q-type cluster analysis classified the herbaceous peony cultivar resources into 3 groups,and the number of cultivars in group Ⅰ accounted for 37.13%,which mainly showed late phenological period. The number of cultivars in group Ⅱ accounted for 32.93%,which mainly showed strong growth potential and large flower diameter. The number of cultivars in group Ⅲ accounted for 29.94%,which was mainly characterized by strong scale bud differentiation ability and large number of flowers. All the quantitative traits,except for the single flowering period,differed to varying degrees among the groups of cultivar resources. Principal component analysis allowed the generalization of the traits with the highest contribution to phenotypic variation into phenological period factor,leaf factor,flowering period factor,flower factor,and growth factor.

    Analysis and Evaluation of Heat Tolerance of Actinidia arguta Cultivars in Extreme Arid Area
    WANG Haizhen, YING Yaolin, WANG Yuqing, LÜ Ruiheng, HAN Lu
    Acta Horticulturae Sinica    2024, 51 (12): 2857-2870.   DOI: 10.16420/j.issn.0513-353x.2023-0676
    Abstract621)   HTML24)    PDF (1289KB)(110)      

    Using three Actinidia arguta cultivars as experimental materials,the relative electrical conductivity(REC),gas exchange and chlorophyll fluorescence parameters,physio-biochemical indexes and field growth performances,heat injury index under artificial-manipulated and ambient high temperature stresses were measured. The heat tolerance was evaluated using fuzzy membership functions over multiple indictor varaibles. The results showed that the relationships between REC and temperature in three tested cultivars conformed well with the Logistic equation. The variation in REC of all A. arguta cultivars with artificial-manipulated increasing temperature followed a S shaped curve. The semi-lethal temperature(LT50)for‘Longcheng 2’‘Minda’and‘Kangtian’were estimated to be 44.60,41.92 and 39.69 ℃,respectively. The failure time of‘Longcheng 2’and‘Minda’under their LT50 were 85 and 76 min,respectively. REC,chlorophyll fluorescence parameters,malondialdehyde(MDA)content and free proline(Pro)content of the three cultivars showed little adjustemnts at < 35 ℃,while photosynthesis and physiological metabolism were drastically mediated at > 40 ℃,resulting in increased membrane peroxidation degree and decreased photosynthetic efficiency. Meanwhile,maximum photochemical efficiency(Fv/Fm),actual photochemical efficiency(ΦPSⅡ),electron transport rate(ETR),photochemical quenching(qP),total chlorophyll content[Chl(a + b)],net photosynthetic rate(Pn),water use efficiency (WUE),transpiration rate(Tr)and Pro of‘Longcheng 2’were the highest among three cultivars,and significantly higher than those of‘Kangtian’. On the contrary,‘Kangtian’was the highest with respect to the yield for dissipation by down-regulation(YNPQ),the yield of other non-photochemical losses(YNO),the relative limitation of photosynthesis(LPTF),MDA and REC,indicating that its’ PSⅡ reaction center had been most damaged by high temperature. In the growing season,field-grown‘Longcheng 2’had faster growth in shoot length,base diameter,leaf thickness and higher relative chlorophyll content(SPAD),and its growth rate was significantly higher than that of the other two cultivars,with slighter leaf damage and the lowest heat injury index under high temperature. LT50 was positively correlated with ΦPSⅡETR and qPP < 0.05),and negatively correlated with LPTF and MDA(P < 0.01). Heat injury index was negatively correlated with Pn,Chl(a + b),WUE,Fv/Fm,Pro,ΦPSⅡ and qPP < 0.01),but positively correlated with REC and MDA(P < 0.01). Simutenously,the heat injury index was negatively correlated with growth rate and LT50P < 0.01),and the LT50 was positively correlated with the growth rate(P < 0.01). The rank of heat tolerance in terms of semi-lethal temperature was consistent with the results of a comprehensive evaluation based on physiological indexes and heat injury index. These results indicate that REC,photosynthetic parameters,physio-biochemical indexes and heat injury index can be used to indicate heat tolerance of A. arguta cultivars. Altogether,the rank of heat tolerance was determined as follow:‘Longcheng 2’(strong heat tolerance)>‘Minda’(medium heat tolerance)>‘Kangtian’(weak heat tolerance).

    Development and Application of Insertion-Deletion(InDel)Markers in Gannan wild Fortunella hindsii Based on Whole Genome Re-sequencing Data
    LUO Sifang, YAN Xiang, XIE Lifang, SUN Jingxian, GUO Zijing, ZHANG Zuming, CHEN Zhaoxing
    Acta Horticulturae Sinica    2025, 52 (2): 267-278.   DOI: 10.16420/j.issn.0513-353x.2024-0357
    Abstract609)   HTML66)    PDF (2040KB)(246)      

    To clarify the InDel characteristics of Gannan(Southern Jiangxi Province)wild Fortunella hindsii,99 Gannan wild F. hindsii were selected for whole genome resequencing,and then InDel variant sites were screened to develop InDel markers. A total of 590.58 Mb valid fragment data was obtained,and 211 470 InDels were detected,including 98 043 insertions and 113 427 deletions. From 8 760 InDel markers with Insertion or Deletion base numbers greater than 10 180 polymorphic markers with uniform distribution on chromosomes were selected,and 58 pairs of InDel primers with good amplification bands were developed. The findings of 58 pairs of InDel markers demonstrated a similar grouping pattern with the clustering results obtained from whole genome resequencing. Additionally,the 99 Gannan wild F. count of core InDel marker sites and alleles present in each germplasm,a total of 29 Gannan wild F. hindsii were selected as the core germplasm. These germplasm samples had a genome coverage of 99.7%. Among them,Jiahu,Yukeng,and Caifang were recognised as the core germplasm regions. The DNA fingerprint was created by integrating the entire genome resequencing data with the core InDel marker sites. This fingerprint contains coding information such as plant number,genotype,mutation locus,and agronomic trait data. This study aimed to elucidate the InDel characteristics of Gannan wild F. hindsii. The identified InDel markers have potential applications in population structure analysis,core germplasm screening,and DNA fingerprint construction.

    Characterization of PtSARD1 and Its Preliminary Role in Drought Tolerance
    MO Jinxia, LI Fang, XIONG Xinting, ZHONG Zaofa, PENG Ting
    Acta Horticulturae Sinica    2025, 52 (6): 1399-1411.   DOI: 10.16420/j.issn.0513-353x.2024-0478
    Abstract609)   HTML94)    PDF (3505KB)(334)      

    Systemic Acquired Resistance Deficient 1SARD1)is a transcription factor related to salicylic acid synthesis. In order to investigate its role in drought response,the 1 425 bp open reading frame of SARD1 in Poncirus trifoliata(named as PtSARD1)was obtained. The characterization of PtSARD1 was preliminarily studied by sequence analysis,subcellular localization,relative expression level,overexpression(OE),RNA interference(RNAi)and tolerance-related physiological indicators measurement. The results revealed that PtSARD1 contained three calmodulin binding protein domains and the subcellular localization of this protein will change over time,transferring from the nucleus to the cell membrane. The expression of PtSARD1 was detected in root,stem and leaf,with higher expression in leaf. Meanwhile,PtSARD1 was induced by drought and exogenous application of salicylic acid. PtSARD1-RNAi P. trifoliata and PtSARD1-OE tobacco were obtained to study its function in drought response. The detached leaves of two PtSARD1-RNAi P. trifoliata lines showed higher relative water loss at each time point. Relative water loss in detached leaves of two PtSARD1-OE tobacco lines(A7 and F7)were lower than that of the wild type tobacco(WT). When water was withheld,potted seedlings of A7 and F7 showed better growth status,higher proline content,but lower electrolyte leakage and malondialdehyde content. Overall,these results indicated that PtSARD1 may play a positive regulatory role in the drought response of P. trifoliata.

    The Rapid Development of China’s Chili Pepper Industry Over the Past 40 Years
    ZOU Xuexiao, YANG Sha, DAI Xiongze, HU Bowen, XU Hao, ZHU Fan, PEI Songyu, YUAN Fang
    Acta Horticulturae Sinica    2025, 52 (1): 247-258.   DOI: 10.16420/j.issn.0513-353x.2024-0805
    Abstract574)   HTML28)    PDF (786KB)(276)      

    In the early 1980s,the annual chili pepper cultivation area was less than 266 700 hm2 in China. After two decades of development,the area reached 1 333 300 hm2 by 2000,reached 21 466 700 hm2 in 2015,maintaining stability and becoming the largest vegetable crop in China. Based on production practices and research surveys,the authors review the major trends in the rapid development of China’s chili pepper industry over the past 40 years and outline the future direction.

    Analysis of Endogenous Microbial Community Diversity,Structure and Function of Pepper Different Resistant Phytophthora Blight Cultivars
    CAI Zeyan, ZHANG Mingxing, ZHOU Chi, TAO Yu, YANG Sha, LI Xin, LI Xuefeng
    Acta Horticulturae Sinica    2025, 52 (2): 322-336.   DOI: 10.16420/j.issn.0513-353x.2024-0620
    Abstract561)   HTML12)    PDF (2186KB)(109)      

    The total DNA of the roots,stems and leaves samples of peppers were extracted from the susceptible pepper cultivars(Fudijian and Qiemen)and resistant pepper cultivars(CM334 and SJ-0948) respectively,and the v3-v4 region of bacterial 16S rRNA gene and the fungal ITS1 region were sequenced using the Illumina platform. The results showed that there were significant differences in the composition of endophytic microorganisms in different pepper cultivars under natural planting. The diversity and richness index of fungal communities in roots,stems and leaves of susceptible cultivars. were significantly lower than those of resistant pepper cultivars. At the phylum level,the dominant bacterial and fungal phyla in the rhizosphere,root and leaves between two types of resistant peppers were the same,but the relative abundance of fungal Ascomycota phylum in the roots,stems and leaves of susceptible pepper(Fudijian)was much higher than that of resistant cultivars at the genus level,the dominant bacterial genera of the resistant pepper(CM334)were Mycobacterium(36.96%)in the root,and 2013Ark19i(15.21%),Photobacterium(5.50%),and Methylobacterium_Methylorubrum(4.11%)in stem,and Aureimonas (3.02%)in the leaves. Moreover,there were more harmful fungal genera in the endogenous parts of susceptible pepper(Fudijian),such as Fusarium accounting for 9.50% and 4.23% in roots and stems,and Cladosporium accounting for 45.46% and 51.74% in stems and leaves,respectively,which were significantly higher than those of two resistant cultivars. Among the fungal genera,only the relative abundance of Neoascochyta(root,leaf)and Mortierella(root)in resistant pepper cultivars was slightly higher than that of the susceptible pepper cultivars. In addition,the analysis of fungal community trophic types through the Funguild database showed that the relative abundance of the fungal communities in pathotroph in the stems and leaves of susceptible pepper(Fudijian)was higher than that of resistant pepper (SJ-0948 and CM334).