Based on the analysis of nationwide climatic data in recent 40 years, the software Arcgis was adopted to map the variation of Frost Free Period (FFP),Aridity index(AI)) and Climatic zoning of Chinese viticulture. By the research we found that: in recent 40 years, especially after 1980s, compared to FFP, the variation of Aridity index was not so significant, and represents a dry-moisture switch in 10 year span. The area suitable for grape-growing in China moved northwards constantly, and compared to the variation of Aridity index, the variation of viticulturable area was much more due to the variation of FFP.
The parameters of root architecture, total root length, surface area, volume, tips, branching angle and fractal dimension etc, were analyzed in young seedlings of Malus hupehensis Rehd. under nitrogen-deficient or iron-deficient in a solution culture system. The results showed that the proportion of fine root increased significantly, the root diameter and the fractal dimension decreased significantly, the angle between main root and first lateral root enlarged, and root tends to horizontal distribution under nitrogen-deficient or iron-deficient condition. Under nitrogen-deficient condition, the total length, surface area and volume of root and the number of Second lateral roots increased significantly. Under iron-deficient condition, the number of root tip and the length of main root increased significantly, the activity and the number of root decreased significantly. The root fractal dimension in iron-deficient condition is less than that in nitrogen-deficient.
The main research of this paper focused on the effects of hot air treatment (38°C 4d) on the wound healing of 'Gala' and 'Red Fuji' apple fruits. SEM (Scanning electron microscopy) analysis found that the epicuticular wax was melted by heat treatment, and then filled in the micro-wounds and encapsulated the hyphae, thereby, prevented the invasion by fungi. Artificial wounds cured for 4 days either at 20°C or 38°C before inoculation could decrease susceptibility to infection, and thereby, the decay development was reduced. But the effects of heating on the wound healing in apples were cultivar dependent. Compared to curing at 20°C, curing at 38°C accelerated the wound healing in 'Red Fuji' apple, but retarded the wound healing in 'Gala' apple.
In view of nitrate accumulation Chinese chive production, effects of exogenous salicylic acid on nitrate accumulation and reduction and assimilation in the leaves of Chinese chive were studied. The results indicated that the nitrate content of Chinese chives were significantly decreased from 15.9% to 21.6% with various concentration SA treatment after 6 to 9 days under different levels of nitrogen. Optimal concentration of SA is 3.0 mmol·L-1, and SA concentration reached 10.0 mmol·L-1, nitrate accumulation could be aggravated. After 9 days with spraying 3.0 mmol·L-1 SA on leaves, the activity of nitrate reductase (NR) and glutamine synthetase (GS) were increased 48.9% and 40.3%; photosynthetic rate and dissolvable protein were increased 43.4% and 21.3%; However, the activity of GOT and GPT were slightly higher than the control. Therefore, activity of NR (which was named as 'N-reducing-driving-pump') and GS (which was called 'first driving pump') were significantly increased by spraying on leaves with exogenous SA, while photosynthesis and transaminations were played the positive role of coordination, and translation of nitrate into free amino acid and dissolvable protein were promoted and nitrate accumulating into the vacuole of Chinese chives were reduced.
Nine samples possibly from genus Tospovirus were collected from major vegetable-growing field in Yunnan province, including Honghe, Chuxiong and Kunming, during 2005 - 2006 period. Around 500 bp fragments were obtained after RT-PCR were executed on 9 samples using Tospovirus Group Primers. The sequence results of around 500 bp fragments were compared in the NCBI BLAST website, one sample from tomato in Honghe county of Yuannan province was identified as Tospovirus genus. A phylogenetic tree was constructed using DNAMAN software according to the sequence of the sample and their corresponding public RNA L fragment sequences of Tospovirus. The results indicated that this sample obtained in this study fell into the Serogroup Ⅳ.
An aseptic seed germination protocol for interspecific hybrid between Cymbidium hybrida × C. sinense and C. faberi was established. The results showed that seed germination rate related to its' mature degree and seed harvested 240 days after pollination germinated well. The suitable medium for hybrid seed germination was half strength MS basal medium supplemented with 0.5 mg·L-1 6-BA and 2.0 mg·L-1 NAA. Germinated seeds can be multiplied in vitro via 2 pathways: PLBs and rhizomes. The optimal medium for proliferation of PLBs was half strength MS medium (Solid/Liquid) supplemented with 0.5 mg·L-1 6-BA and 2.0 mg·L-1 NAA, and proliferation coefficient could reach to 4.25 after 30 days culture. The development and plantlet regeneration of PLBs can be highly enhanced on medium containing 1.0 mg·L-1 6-BA and 0.5, 1.0 mg·L-1 NAA. On the other hand, when rhizomes developed from PLBs were cultured on the medium containing 0.5 mg·L-1 6-BA and 1.0 mg·L-1 NAA for 40 days, the highest proliferation coefficient reached to 6.67, and subsequently more than 80% of the apex of rhizomes developed into shoots within 70 days culture on the medium containing 1.0 mg·L-1BA and 0.5 mg·L-1 NAA.
Malus sieversii (Lebed.) Roem., the main progenitor of domesticated apple (Malus domestica Borkh.) is rich in genetic diversity. M. sieversii is mainly distributed in Tianshan Mountains in Central Asia including Gongliu population, Xinyuan population, Huocheng population and Yumin population in China.The occurrence,taxonomic status,Population genetic structure,Genetic diversity and Core collection construction of Malus sieversii were reviewed ,in order to provide the reference for efficacious conservation and utilization of Malus sieversii.
Conditions for pretreatment and determination of organophosphorus pesticide residues contained in fruits of Citrus unshiu Marc. 'Guoqing 1' were optimized. Furthermore, the dynamical regularity of chlorpyrifos in citrus fruits was studied. The results showed that pretreatment techniques were suitable for citrus sample. The limits of detection for dimethoate, methidathion and chlorpyrifos were 0.244,0.306 and 0.409 μg·mL-1 respectively,the average recoveries were from 74.76 to 99.16 %,and the relative standard derivation (RSD) were between 1.01 % and 10.08 %,which met the requirements of multiple residues analysis in a single run. More pesticide residues and faster dynamical regularity of chlopyrifos were found in the peel than in the pulp of citrus,and almost no residue was found in fruits after 30 days of spraying. The half-life of the pesticide was significantly different between the citrus fruits treated with different pesticide concentrations. The degeneration of chlorpyrifos residue in citrus peel and pulp were 10.71 ~ 10.88 d and 13.55 ~ 13.85 d respectively.