Genetic Diversity and Core Collection Construction
of Phalaenopsis Germplasm Resources

doi:10.16420/j.issn.0513-353x.2024-0511

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (6): 1519-1529.doi: 10.16420/j.issn.0513-353x.2024-0511

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles

Genetic Diversity and Core Collection Construction of Phalaenopsis Germplasm Resources

LI Zuo，XIAO Wenfang^*（smilehigh126@126.com），CHEN Heming，and LÜ Fubing^*（13660373325@163.com）

Environmental Horticulture Research Institute of Guangdong Academy of Agricultural Sciences，Guangdong Key Lab of Ornamental Plant Germplasm Innovation and Utilization，Guangzhou 510640，China

Received:2025-02-21 Revised:2025-03-28 Online:2025-06-25 Published:2025-06-20
Contact: XIAO Wenfang，LÜ Fubing

Abstract

Abstract: This study employed fluorescence-based SSR marker technology to conduct a systematic genetic diversity analysis and core germplasm construction of 412 Phalaenopsis germplasm accessions. A total of 124 alleles（N_a）were detected using 10 pairs of SSR markers，with an average number of effective alleles（N_e）of 2.489 and a Shannon’s Information Index（Ⅰ）of 1.209，indicating a high level of genetic diversity in the Phalaenopsis germplasm resources. Based on this，core collection was selected using the maximisation strategy method，Core Finder software，and t-tests，resulting in the identification of 53 core collection，representing 13% of the original germplasm collection. This core set retained 100% of the alleles（N_a），138% of the effective alleles（N_e），and 127% of the Shannon’s information index（Ⅰ）. The results of the t-test showed no significant differences in the genetic parameters between the constructed core collection and the original collection，demonstrating that the constructed core collection effectively represents the genetic diversity of the original Phalaenopsis germplasm resources.

Key words: Phalaenopsis, germplasm resources, SSR, genetic diversity, core collection

CLC Number:

S 682.31

LI Zuo, XIAO Wenfang, CHEN Heming, LÜ Fubing. Genetic Diversity and Core Collection Construction of Phalaenopsis Germplasm Resources[J]. Acta Horticulturae Sinica, 2025, 52(6): 1519-1529.

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Genetic Diversity and Core Collection Construction of Phalaenopsis Germplasm Resources

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