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2009, Vol.36, No.2 Previous Issue    Next Issue

果树

  • The Differences Between Relative Light Intensity and Yield , Quality in Two Canopy of Sweet Cherry
  • WANG Xiao-wei;SHANG Zhi-hua;ZHANG- Qiang;WEI Qin-ping;and LIU-jun
  • Acta Horticulturae Sinica. 2009, 36(2): 157-162.
  • Abstract ( 1878 ) HTML ( 1503 ) PDF (480KB) ( 1503 )    
  • The method of space subarea and dynamic monitor of relative light intensity within sunlight and overcrowed canopy of the 13 years-old trees of ‘hongdeng’sweet cherry were applied to study the distributive difference of relative light intensity, yield and quality, and relationships between relative light intensity and quality. The results showed that the relative light intensity increased gradually from bottom to top, the percent proportion of <35% relative light intensity in volume of canopy were separately 25.23% and 52.78%. The distribution of fruit were uniform and centered on 1.5-2.5m height in the sunlight canopy., while that were difference and outer positions of the canopy in the overcrowed canopy, and the yields were 9.02 t.hm-2 and 3.53 t.hm-2 in sunlight and overcrowed canopy respectively. The regression analysis of the relationship between relative light intensity and fruit quality factors was used to obtain optimum relative light intensity values that mean fruit mass, fruit firmness, soluble solids content, titratable acid and TSS/TA were 76.52%, 46.84%, 100.00%, 41.63% and 75.77% respectively.
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  • Effects of Root-zone Hypoxia Stress on Growth and Endogenous Hormones in Young Kiwifruit Seedlings with Different Resistant Ability
  • MI Yin-fa;MA Feng-wang;and MA Xiao-wei
  • Acta Horticulturae Sinica. 2009, 36(2): 163-170.
  • Abstract ( 2187 ) HTML ( 1429 ) PDF (1395KB) ( 1429 )    
  • Young kiwifruit seedlings(Actinidia deliciosa and A. chinensis)were planted in nutrient solutions aerated with nitrogen. Under root-zone hypoxia stress, the growth and response of endogenous hormones abscisic acid (ABA), indole-acetic acid (IAA), zeatin riboside (ZR), gibberellins (GA3) in kiwifruit seedlings were studied. The results showed that the fresh and dry weight of seedlings treated under hypoxia were less than those of control. Effects of hypoxia stress on A. deliciosa were much slighter than on A. chinensis, which made A. deliciosa perform a better resistance. The ABA contents in A. deliciosa, A. chinensis leaves and roots were enhanced obviously, namely raised by 289.2%, 412.9%, 188.5%, 177.4%, respectively. The IAA contents in A. deliciosa, A. chinensis leaves were raised to the maximum on the 6th day, then reduced but still higher than those of control, namely raised by 87.3%, 61.4%, respectively. Same tendencies happened in the roots of both species, but the peak appeared on the 4th day and differed from that for the leaves. The IAA contents in roots of A. deliciosa, A. chinensis were increased by 90.4%, 59.6%, respectively. The GA3 and ZR contents in A. deliciosa, A. chinensis leaves were decreased by 56.5%, 79.0%, 70.5%, 86.1% correspondingly. The GA3 and ZR contents in A. deliciosa, A. chinensis in roots were lessened by 33.1%, 55.1%, 46.9%, 71.3% correspondingly. The ratios of IAA/ABA, ZR/ABA, GA3/ABA were also studied and the results of them decreased, which showed that kiwifruit seedlings were obviously inhibited from growing under hypoxia and the resistant ability had an intimate relationship with endogenous hormones.

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  • Methyl Jasmonate-Induced Defense Responses of Apple Suspension Cells
  • LIU Zhi;;ZHANG Hua-Lei;XIE Xing-Bin;SHU Huai-Rui;and HAO Yu-Jin;
  • Acta Horticulturae Sinica. 2009, 36(2): 171-178.
  • Abstract ( 2157 ) HTML ( 1215 ) PDF (124KB) ( 1215 )    
  • Meythyl Jasmonate (MeJA) is an elicitor for defensive responses of plant to pathogens, while NO and H2O2 are signal molecules related to defensive responses. In this study, MeJA-induced defense responses of apple suspension cells were monitored in NO release, H2O2 accumulation, PAL activity and MDA content, with sodium nitroprusside (SNP) as a NO donor, L-NAA (Nx-nitro-L-arginine) as NOS inhibitor and PTIO (2-phenyl-4,4,5,5-tetramethyl- imidazoline-1-oxyl-3-oxide) as NO scavengers. The results showed that apple suspension cells constantly produced NO and reached a peak release at 6h when exposed to MeJA treatment. On the contrary, L-NAA and PTIO inhibited the generation of NO in cells. MeJA treatment also led to a great increase in H2O2 level in suspension cells, but SNP treatment did not. In addition, PAL activities as well as MDA and phytoalexin contents displayed similar dynamic curves with different treatments, increasing firstly and then decreasing with peak activity or content at 4-6h. Those findings indicated that MeJA induced the generation of NO, and then MeJA and NO worked together to increase H2O2 level in suspension cells. Phytoalexin and MDA increase as well as PAL activity enhancement were mainly induced by NO, although it seemed other signals involved.


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  • Genetic Linkage Maps Constructing and Markers Linked with Pistil Development and Double Petal Gene in Peach
  • CAO Ke;WANG Li-rong;ZHU Geng-rui;FANG Wei-chao;and CHEN Chang-wen
  • Acta Horticulturae Sinica. 2009, 36(2): 179-186.
  • Abstract ( 2115 ) HTML ( 2385 ) PDF (683KB) ( 2385 )    
  • A linkage map of peach was constructed with SSR , AFLP and SRAP markers in 52 F1 population derived from the cross of Prunus persica (L.) Batsch. Hongchuizhi and P. davidiana (Carr.) Franch. Baihuashanbitao. The selected primers including 38 SSR, 61 AFLP and 38 SRAP with rich polymorphism and steady bands were tested in progeny. 441 markers in 1:1 ratio by X2 test and 52 markers for double heterozygous loci were scored. Mapmaker software were used to assigned the markers in 1:1 ratio and pistil abortion trait to 11 linkage groups, the map is composed of 206 loci (18 SSRs, 126 AFLPs, 61 SRAPs, and one morphological trait) distributed in 11 linkage groups, the map covers 1193.2 cM of the peach genome. The average length of 11 groups is 108.5cM, the average distance between adjacent loci is 5.8 cM. Eighteen SSR loci in common with a saturated reference linkage map (T×E) of Prunus allowed a comparative analysis to be made between the two maps, homologies were found among the respective linkage groups, the map in this study showed better identity with peach reference map. Pistil development trait, were placed on the group1(Chromosome 1). Two AFLPs linked to a new double petal gene were obtained through analysis of the markers for double heterozygous loci.
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  • GC/MS Analysis of Volatile Components in High-bush Blueberry Cultivars
  • ZHANG Chun-yu;LI Ya-dong;CHEN Xue-sen;ZHANG Zhi-dong;LIU Hai-guang;and WU Lin
  • Acta Horticulturae Sinica. 2009, 36(2): 187-194.
  • Abstract ( 2564 ) HTML ( 2213 ) PDF (462KB) ( 2213 )    
  • The aroma components of high-bush blueberry were analyzed using early, middle, later cultivars by static headspace and gas chromatography-mass spectrometry (GC-MS). Results showed that the main volatile compounds were alcohols, esters, and terpenes for high-bush blueberry. The sixty-seven kinds of volatile components were detected and there were distinct differences between the cultivars, in which 16 kinds, 17 kinds, 27 kinds, 13 kinds, 26 kinds, 25 kinds were detected in Duke, Bluejay, Bluecrop, Jersey, Elliot, Darrow, respectively. The character impact components (CICs) of Duke were ethyl butanoate, damascenone, ethyl 2-methylbutyrate, D-limonene, and 2-butanone. The CICs of Bluejar were ethyl 2-methylbutyrate, ethyl butanoate, and butyl acetate. The CICs of Bluecrop were ethyl 2-methylbutyrate, (E)-3-Hexenyl acetate, β-linalool, ethyl butanoate, hexyl acetate, (E)-2-hexenal, and (Z)-3-hexen-1-ol. The CICs of Jersey were ionone, ethyl 2-methylbutyrate, ethyl butanoate. The CICs of Elliot were hexyl acetate, β-linalool, D-limonene, hexanal. The CICs of Darrow were ethyl butanoate, hexyl acetate, D-limonene, andβ-linalool. The unique flavor of high-bush blueberry cultivars was formed because of interaction between these CICs.
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蔬菜

  • Response of Anatomical Structure and Photosynthetic Characteristics to Low Light in Leaves of Capsicum Seedlings
  • SUI Xiao-lei;MAO Sheng-li;WANG Li-hao;LI Wei;ZHANG Bao-xi;and ZHANG Zhen-xian
  • Acta Horticulturae Sinica. 2009, 36(2): 195-208.
  • Abstract ( 2579 ) HTML ( 2022 ) PDF (2730KB) ( 2022 )    
  • Four genotypes of capsicum (Capsicum annuum L.) with distinct low light tolerance were used to study the response of anatomical structure, ultrastructure of chloroplast,stomatal characteristics and photosynthetic characteristics of leaves to low light (75~100 μmol·m-2·s-1) in a phytotron. The results revealed that leaves treated with low-light became thinner with an increase of palisade/spongy tissue ratio, and two genotypes including hot pepper ‘Fudijian’and sweet pepper ‘Shanghai Yuanjiao’had higher ratio than the other two. Under low light, the number of chloroplast decreased, but the size of chloroplasts and starch grains, the number of grana, grana lamella, starch grain and grana thickness of chloroplast increased. Comparing with sweet pepper, hot pepper had more chloroplasts and granas. In sweet pepper, the stomatal density in lower epidermal descended during leaf unfolding, but no more changes of stomatal index and number of stomata per leaf occurred after 14 day of leaf unfolding. Low light treatment resulted in decreases of stomatal density, stomatal index and number of stomata per leaf, and increases of the stomatal size, the vertical and horizontal axes of stomata in ‘Shanghai Yuanjiao’. Simultaneously, the longer horizontal axes of stomata were also observed in another genotype ‘20078’. The epidermal cells of sweet pepper grown under low light were large and distorted. Meanwhile the stomata and lower epidermal cell were equal height or the stomata slightly protruded out of the epidermal wall. The cycle wrinkle could be obviously observed on cuticle of guard cell in lower epidermis of leaves under low light. Photosynthetic parameters including light saturated rate of the CO2 assimilation (Asat), dark respiration rate (Rd), night respiration rate (Rn), light respiration rate (Rp), light compensation point (LCP), light saturation point (LSP), CO2 compensation point (Г), CO2 saturation point (CSP), carboxylation efficiency (CE) and maximum RuBP regeneration rate decreased, while apparent quantum yield (Фi) increased under low light. The Asat decreasing, LCP, LSP, Rd, Rn and Rp were lower in hot pepper than those in sweet pepper. The start time of photosynthesis (namely, the net photosynthetic rate i.e. Pn from zero to maximum) in low-light leaves was relatively prolonged, especially in low light-sensitive than in low light-tolerant genotypes. Hence, some adaptive response of photosynthetic characteristic to low light have happened in capsicum leaves.

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  • Inter-specific Hybridization between Solanum melongena and Solanum torvum
  • CAO Bi-hao;LEI Jian-jun;WANG Yong;LU Ya-qing;and CHEN Guo-ju
  • Acta Horticulturae Sinica. 2009, 36(2): 209-214.
  • Abstract ( 1943 ) HTML ( 1913 ) PDF (566KB) ( 1913 )    
  • In order to obtain the new disease-resistance resource in eggplant, inter-specific hybridization was made with pollination between Solanum torvum and Solanum melongena inbreeding lines,‘E-8’,‘E-30’, ‘E-35’,‘E-38’. Only the inter-specific hybrid of ‘E-35’×Solanum torvum was obtained. The hybrid were identified by morphology , RAPD and GISH methods, the disease-resistance of the hybrid was tested by inoculation .The results showed that the morphological characteristic of F1 hybrid was intermediate to that of its parent , the pollen viability of F1 hybrid was high , its seed was fertile. Not only its parent characteristic bands, but also some new characteristic bands of hybrid were observed in F1 hybrid plant , and the chromosome of both parent (Solanum melongena ‘E-35’and Solanum torvum) were determined in F1 hybrid , its NO. of chromosome was about 2n = 24,those showed that the F1 hybrid was a real inter-specific hybrid. The hybrid showed high bacterial wilt resistance by inoculated to race 1 of bacterial wilt and in the field, the resistance to bacterial wilt may be controlled by two dominance genes. So the inter-specific hybrid can be used as a new resource with resistance to bacterial wilt for eggplant breeding .
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  • Effects of Low-temperature Stress on the Ascorbate-glutahione Cycle in the Grafted Cucumber Leaves
  • GAO Jun-jie;QIN Ai-guo;and YU Xian-chang
  • Acta Horticulturae Sinica. 2009, 36(2): 215-220.
  • Abstract ( 2099 ) HTML ( 1666 ) PDF (1209KB) ( 1666 )    
  • To make clear the intrinsic mechanism of cold resistance of grafted cucumber,changes of relative expression and activities of antioxidant enzymes in the AsA-GSH circle and the contents of AsA, GSH and H202 were tested in the leaves of either own-root cucumber (Junlv No. 3) or grafted plants where cucumber (Junlv No. 3) was grafted on black-seed pumpkin. Both plants were treated under low-temperature stress. The results showed that, under low-temperature stress, the relative expressions of DHAR, and GR mRNA were all higher in the leaves of grafted cucumber than that of own-root cucumber, while no significant changes were found in the relative expressions of GalLDH and APX mRNA; the contents of AsA, GSH and the ratio of AsA/DHA, GSH/GSSG were all higher in grafted cucumber than that of own-root cucumber, while the contents of DHA, GSSG and H2O2 were all lower. We concluded that the higher activities of DHAR and GR, which were from the higher relative expression of DHAR and GR mRNA, were mainly responsible for the higher contents of ASA and GSH in the grafted cucumber leaves. But the higher activities of GalLDH and APX have nothing to do with the relative expression of GalLDH and APX.

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  • Embryoid Induction and Plant Regeneration of Cucumber (Cucumis sativus L.) through Microspore Culture
  • ZHAN Yan;CHEN Jin-feng;and AHMED Abbas Malik
  • Acta Horticulturae Sinica. 2009, 36(2): 221-226.
  • Abstract ( 2795 ) HTML ( 1821 ) PDF (349KB) ( 1821 )    
  • Isolated microspore culture in cucumber was studied by using 10 different varieties of cucumber. Cotyledonary embryoids and plantlets were obtained from '7447' and 'Poinsett97' genotypes for the first time. The results showed that genotype and the developmental stage of microspore were the key factors in embryoid induction. The embryoid yield was different significantly among different genotypes. Embryoid yield per culture dish varied from 1.5~33.4. The late-uninucleate stage of microspore was found optimum for isolated microspore culture in cucumber. Cold-pretreatment (4 ℃) for 2~4 days also promoted embryoid induction, while the highest embryoid yield was obtained form microspore pre-treated at 4 ℃ for 2 days. The results also indicated that low concentration of exogenous hormones (0.5 mg·L-1 2,4-D and 0.2 mg·L-1 6-BA) were conductive to embryogenesis, whereas NLN and B5 medium had no significant difference on embryoid induction. The regenerated plantlets were obtained only from cotyledonary embryoids and no plantlet was produced from the embryoids at other developmental stages.

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  • Comparative Mapping of Virus Resistant Gene zym-CH in Watermelon
  • XU Xiang-li;GUO Shao-gui;GONG Guo-yi;XU Yong;LU Xiang-yang;PENG Zhen-xing;and ZHANG Hai-ying
  • Acta Horticulturae Sinica. 2009, 36(2): 227-232.
  • Abstract ( 1763 ) HTML ( 1780 ) PDF (695KB) ( 1780 )    
  • The watermelon germplasm P.I.595203 resistant to ZYMV-CH ( Zucchini Yellow Mosaic Virus-CHINA ) and 98R susceptible to ZYMV-CH were used as the parents to construct F2 population and F2:3 population. The markers linked to the resistant gene zym-CH were filtered by BSA strategy.The reference genetic map constructed basing on the F2S8 population(P.I.296341-FR×97103)was used for comparative mapping of zym-CH gene. The results showed that b13-1400, MCPI-14 and MCPI-26 were linked to zym-CH gene with the genetic distance of 25.6 cM, 57cM and 87cM respectively. The resistant gene zym-CH was located on 105 cM of the linkage group 3 by the collinearity relationship of zym-CH, b13-1400, MCPI-14 and MCPI-26 in the two linkage maps.
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观赏植物

  • Effects of Waste Crumb Rubber Filled in Turf Medium on Biological Characteristics of Lolium perenne L. before and after Trample Stress
  • ZHAO Shu-lan;DUO Li-an;and WANG Li-li
  • Acta Horticulturae Sinica. 2009, 36(2): 233-238.
  • Abstract ( 1944 ) HTML ( 1273 ) PDF (278KB) ( 1273 )    
  • The biological characteristics such as the number of nodes, branch length, coverage, the number of leaves, tillers, overground biomass and root growth of Lolium perenne L. before and after trample stress were investigated by establishing turf system with waste crumb rubber as medium amendment. After lawn returned green in the second year, the number of nodes and coverage of Lolium perenne L. increased with the decrease of crumb rubber size, and all crumb rubber treatments were all distinctly higher than the treatment without crumb rubber (control) before trample stress, but the branch length showed the opposite trend, increased with the increase of crumb rubber size. Under trample stress, the coverage, overground biomass and tiller number of ryegrass (L. perenne )displayed the same rule. All of the parameters increased as decreasing crumb rubber sizes. The treatment with 1-2 mm appeared the highest, and all treatments with different crumb rubber sizes were better than control. Trample stress damaged the number of nodes and leaves greatly. In addition, the root biomass with 1-2 mm crumb rubber treatment under trample stress was the highest, and was higher 138.77% than control. So the waste crumb rubber filled in turf medium as amendment could enhance trample resistance of lawn based on above comprehensive analyses. This was directly correlated with elasticity of crumb rubber. In the three treatments, the size of 1-2 mm crumb rubber was the optimum medium amendment for sports lawn under trample stress, and the lawn character was clearly better than that of other treatments.
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  • Effect of Ethylene on Cut Flowers of Tree Peony 'Luo Yang Hong' Opening and Senescence Process and Endogenous Ethylene Biosynthesis
  • ZHOU Lin;JIA Pei-yi;LIU Juan;WANG Wei-ran;HUO Zhi-peng;and DONG Li
  • Acta Horticulturae Sinica. 2009, 36(2): 239-244.
  • Abstract ( 2379 ) HTML ( 2043 ) PDF (408KB) ( 2043 )    
  • Tree peony cut flower (Paeonia suffruticosa 'Luo Yang Hong') harvested in the first grade of flower opening index was taken as material to investigate the influence of exogenous ethylene and ethylene action inhibitor 1-MCP on flower opening index, diameter increasing rate, vase life, endogenous ethylene production and ethylene biosynthetic enzymes activities of ACC synthase (ACS) and ACC oxidase (ACO) during postharvest opening and senescence. The results showed that 10 μL·L-1 ethylene treatment for 6 h hastened the process of flower opening, shortened vase life and induced petals abscission seriously after full-open; while 1.0 μL·L-1 1-MCP retarded flower opening, prolonged flower vase life and impeded full-open of some flowers. Endogenous ethylene production was promoted by ethylene and inhibited by 1-MCP respectively, which was closely associated with the flower opening process. ACS and ACO activities analysis after different treatments indicated that changes in ACS activity were related to the endogenous ethylene production, suggesting that ACS is the main factor in the regulation of tree peony cut flower opening and senescence, which confirmed the former conclusion that ACS is the rate-limiting enzyme in plants ethylene biosynthesis.

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  • Identification and distribution inside plant of pathogen causing stem rot of Pandanus veitchii
  • XIAO Rong-feng;LIU Bo;Lin Kang-mei;Zheng Xue-fang;and LAN Jiang-lin
  • Acta Horticulturae Sinica. 2009, 36(2): 251-256.
  • Abstract ( 1992 ) HTML ( 1760 ) PDF (377KB) ( 1760 )    
  • The objectives of this study were to isolate and identify the pathogen which caused stem rot of Pandanus veitchii, and to determine the amount of this pathogen inside the plant. The diseased plants of P. veitchii showing symptoms of stem rot were collected from Jianxin, Fujian province, PR China. 10 isolates of microorganism were obtained and identified as Fusarium verticillioides by Booth's method and sequence of rDNA-ITS. And, the results of pathogenicity testing confirmed that these isolates were pathogen of P. veitchii. Meanwhile, comparative investigation was conducted on the amount of F. verticillioides inside the symptomatic and asymptomatic plants of P. veitchii. It was found that the amounts of the pathogen in the stem were higher than those of in the root and in the leaves. The pathogens in the symptomatic plants were significantly more than those in the asymptomatic plants.
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研究简报

  • Polyploid Induction in Pear in vitro Treatment With Gamma-rays
  • SUN Qing-rong;SUN Hong-yan;ZHU En-yuan;and LI Lin-guang
  • Acta Horticulturae Sinica. 2009, 36(2): 257-260.
  • Abstract ( 1751 ) HTML ( 1344 ) PDF (263KB) ( 1344 )    
  • In vitro shoots of pear cultivar ‘Fertility’(Pyrus communis L.) were irradiated with gamma rays. Three different explants of shoot tip, lateral bud and young leaf from irradiated shoots were cultured separately. Shoots from 3 different explants were subcultured and selected. Morphological mutants with thicker stem, shorter internode, smaller leaf index and shorter petiole were obtained.Chromosome counting confirmed that the mutants were triploids and tetraploids. The lengths of stomatal guard cells and specific leaf mass of polyploids were significantly increased than that of diploids.
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  • Studies on Cold Shock Stress Effect of Photosystem Ⅱ and Its Thermodynamics Analyse in Eggplant
  • WANG Mei;GAO Zhi-kui;HUANG Rui-hong;LV Gui-yun;ZHANG Wen-li and DU Shuang
  • Acta Horticulturae Sinica. 2009, 36(2): 261-266.
  • Abstract ( 2118 ) HTML ( 1220 ) PDF (457KB) ( 1220 )    
  • A fast chlorophyll fluorescence parameters were measured in two varieties of eggplants, Duanbahei with colder tolerance and Heishuai with higher temperature tolerance by Plant Efficiency Analyzer (PEA). Also the thermodynamics analyse of PSⅡ cold shock stability were studied by using the standard stade denaturalization Gibbs free energy variation △GD of the maximal photochemical efficiency of photosystem Ⅱ (Fv/Fm) and reaction centers RC/CSo . With the temperature decreasedand time extended, The Fv/Fm of PSII, factual photochemistry efficiency ΔF/Fm′ and RC/CSo were "S" downtrend.The exhibition of the energy dissipation DIo/RC and the nonphotochemical quenching coefficient qN were "S" ascends.The generalized analysis reflected the dual mechanism under the cold shock, the reversible deactivation of PSⅡ reaction centers and the energy dissipation which depended on lutein circulation were important in protecting PSⅡ for preventing photoprohibition. Moreover, two groups of new parameters were introduced as index for comparing cold shock easily through the different PSⅡ action of two varieties. Semi-attenuation temperature T1/2 and t1/2 of Heishuai were 1.4 ℃ and 0.8 ℃ higher than that of Duanbahei. The △GD of the Fv/Fm and RC/CSo in the leaves of eggplant were "waterfall" downtrend with the cold shock temperature decreased.The △GD and the denaturalization midpoint temperature Tm of Heibei were lower than Duanbahei. The result showed the cold tolerance of Heishuai is lower than Duanbahei.

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  • Studies on Anther Development of Mustard Tubers Cytoplasmic Male Sterile Lines in Chinese Cabbage
  • KONG Yan-e;ZHANG Shu-ning;Hou Xi-lin;LIU Hui-ji;and ZHONG Cheng
  • Acta Horticulturae Sinica. 2009, 36(2): 267-272.
  • Abstract ( 1948 ) HTML ( 1485 ) PDF (586KB) ( 1485 )    
  • Anther anatomical structure and development was observed using a light microscope through paraffin slices on the mustard tubers cytoplasmic male sterility line(CMS) and its corresponding maintainer line in tetraploid Chinese cabbage. The results showed that this allogenic cytoplasmic male sterility belongs to structural male sterility. There were five types in the degraded and abnormal stamens as follows:(1)peltate stamen;(2)stripe stamen;(3)sheet stamen;(4)pinnate stamen;(5)petaloid stamens. The anther development of the tetraploid chinese cabbage CMS was inhibited at two stage. The abortion of peltate stamen anther occurred at the stage of archesporial cell differentiation, at which there was no differentiation of fibrous layer, middle layers, tapetum and microspore mother cell and no anther sac formed. Peltate stamen of the allogenic cytoplasmic male sterility line belongs to non-sporange cytoplasmic male sterility. And the abortion of other types anther might occur at the stage of stamen anlage polarization. The stamen anlage deviated from the normal polarization and formed petal anlage, developing into petal.

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  • Cloning and Expression Analysis of SLG Gene in Brassica campestris L. ssp.chinensis
  • ZHANG Ai-fen;LI Ying;LIU Tong-kun;SHI Gong-jun;and HOU Xi-lin;
  • Acta Horticulturae Sinica. 2009, 36(2): 273-278.
  • Abstract ( 1832 ) HTML ( 1327 ) PDF (1209KB) ( 1327 )    
  • Through RT-PCR technique 1 324 bp cDNA sequence of S locus glycoprotein gene (BcSLG) was obtained from self-incompatible line with specific primers in Brassica campestris ssp.chinensis. Sequence analysis indicated BcSLG contained one open reading frame, which encoded 432 amino acids with 12 conserved cysteine residues and 7 potential N-linked glycosylation sites. Sequence alignment and phyologenetic analysis revealed BcSLG had high similarity to SLG from other plants and had closest phylogenetic relationship to that in Brassica campestris L. and Brassica oleracea L. Real-time PCR analysis showed BcSLG was highly expressed in stigmas, much lower in buds and lowest in leaves of self-incompatible line. However, in self-compatible line BcSLG was expressed in an even lower level whether in stigmas, buds or leaves.

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  • Changes on Physiological Characteristics of Osmanthus fragrans ‘Zibing Ziyingui’Seeds During Dehydration
  • LI Wen-jun and SHEN Yong-bao
  • Acta Horticulturae Sinica. 2009, 36(2): 279-284.
  • Abstract ( 1799 ) HTML ( 1550 ) PDF (681KB) ( 1550 )    
  • The physiological characteristics changes of Osmanthus fragrans‘Zibingziyingui’seeds during natural dehydration were measured to study the seed desiccation sensitivity. The results showed that O. fragrans seeds were desiccation-sensitive as the seed viability declined to 6% when the seed moisture content was reduced to 11.6%. The seed electrical conductivity increased with the declining moisture content, a sign, which was caused by increasing cell membrane cleavage. Superoxide dismutase (SOD) activity decreased rapidly during desiccation; but peroxidase (POD) activity increased to the maximal value when dehydration for 12 h, then declined rapidly when seeds were dehydrated more than 12 h. Superoxide anion radical content decreased with the declining moisture content, but it would increase rapidly when seeds were dehydrated more than 12 h. Malondialdehyde (MDA) content increased as seed moisture content declined, indicating that membrane peroxidation was accelerated and the seed viability would be lost.
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  • Morphological and Anatomical Studies of Floral Organogenesis and Development in Catalpa speciosa
  • CHEN Xu-hui;JIANG Sha;GU Song;XU Ke;WANG Yong-zhou;DING Rui;and HUANG Jun-zhe
  • Acta Horticulturae Sinica. 2009, 36(2): 285-290.
  • Abstract ( 1947 ) HTML ( 1514 ) PDF (1483KB) ( 1514 )    
  • The initiation and development of flower organs in Catalpa speciosa (Warder) Warder ex Engelm were observed by light microscope and scanning electron microscope. The results showed that: (1) The whole process of floral initiation and development lasted about one month from late March to early May. (2) On the floral apex the different classes of organ primordia appeared in the following sequence: calyx, corolla, androecium and gynoecium, and they had overlapped with each other when occurring in turn. (3) The development of anther and ovule followed the finish of the flower organ initiation. Pollen mother cells and well developed microsporagium wall occurred when the diameter of the bud reached about 3.0 mm, and the archesporial cell which played the role of megaspore mother cell formed in ovule when the diameter of the bud reached about 3.5 mm.

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  • A Karyological Study of Six Species of Cymbidium
  • LENG Qing-yun;MO Rao;PEN Bin;HUANG Ming-zhong;LUO Yuan-hua;WANG Jia-bao
  • Acta Horticulturae Sinica. 2009, 36(2): 291-296.
  • Abstract ( 2051 ) HTML ( 1715 ) PDF (962KB) ( 1715 )    
  • The chromosome number and karyotype of five native species and one hybrid cultive of Cymbidium was studied by applying fingertip pressing method. The results showed that the C. floribundum Lindl . 2n=2x=40=36m+4sm ;C. dayanum Rchb. 2n=2x=40=36m+4sm ;C. bicolor Lindl. subsp.obtusum Du Puy et Cribb 2n=2x=40=40m(4SAT);C.insigne Rolfe 2n=2x=40=2M+36m+2sm;C.eburneum Lindl. 2n=2x=40=32m+8sm ;C.golden Elf.‘Sundust’2n=2x=40=34m+6sm(2SAT). Aslo we has discussion the relationship and the role in the orchid breeding of these Cymbidiums.
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综述

新品种

  • A New Red Pear Cultivar ‘Mantianhong’
  • WEI Wen-dong;TIAN Peng;and SU Yan-li
  • Acta Horticulturae Sinica. 2009, 36(2): 303-303.
  • Abstract ( 1847 ) HTML ( 1251 ) PDF (92KB) ( 1251 )    
  • ‘Mantianhong’ is a new pear cultivarwhich derived from the cross‘Huobali’ בKosui.’Fruitsmature in the late Sep tember in Zhengzhou. The fruit is red, large and weighing around 280 g, The flesh is fine, juicy, crisp, sweet2tartwith little stone cell. The soluble solids content is 14.5% - 15.5% , the total sugar content is 9.45% , The titratable acid content is 0.40% and the vitamin C content is 0.0327 mg·g-1. It bears early, has high and stable yield, long shelf life, and resistance to adverse condition, diseases and pests.
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  • A New Peach Cultivar ‘Heluohongmi’
  • YANG Ying-jun;CHU Aixiang;ZHANG Yao-zhan;LI Yan-mei;GUO Guo-ning;and HAO Shui-wang
  • Acta Horticulturae Sinica. 2009, 36(2): 304-304.
  • Abstract ( 1908 ) HTML ( 1259 ) PDF (420KB) ( 1259 )    
  • ‘Heluohongmi’peach is a new peach cultivar bred from the cross of ‘Laishanmi’ ×(‘Okubo’and ‘Dongtao’). The fruit is round. The attractive red blush covers 90% of the skin surface.The average fruit weight is 350 g. The soluble solids content is 1216% - 1318%. The flesh is white, firm,sweet, and freestone with a good eating quality. It ripes in mid2August with a high p roductivity. It can be grown in north China, northwest China and middle China.
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  • A New Pomelo Cultivar ‘Hushouyou’
  • HE Yi-fa;YU Zhan-shen;WANG Ming-hong;YI Yong-mei;ZHOU Guang-lai;and LIU Hua-zhong
  • Acta Horticulturae Sinica. 2009, 36(2): 305-305.
  • Abstract ( 1700 ) HTML ( 1313 ) PDF (73KB) ( 1313 )    
  • ‘Hushouyou’ is high quality and mid2late ripening pomelo cultivarwhich was bred from superior individuals growing in low altitude mountainous area and interfluve of the southwest of Hubei p rovince.The fruit is p ip less or only a few p ip s, and the flesh of fruit can be easily apart with velum of bursa, and is tender and crisp and juicy, and also with a moderate taste of sweet and sour and with little bitterness. ‘Hushouyou’fruits are tolerant to storage, mid2late ripening, high p roducts, good adap tability, and strong fastness.
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  • A New High Lycopene Cherry Toma to Cultivar‘Yinghong 1’
  • WANG Jin-hua;ZHAO Xiao-bin;DING Jun-jie;MI Guo-quan;and CHENG Zhi-fang
  • Acta Horticulturae Sinica. 2009, 36(2): 307-307.
  • Abstract ( 1899 ) HTML ( 1182 ) PDF (166KB) ( 1182 )    
  • ‘Yinghong 1’ is a new cherry tomato cultivar which was selected from crossing of Holland‘Yingtaohong’ ×Japan ‘Baibai’. It has unlimited branches and grows vigorously. The first inflorescence is situated between the eighth and ninth node and each inflorescence bears 30-50 fruits. The single fruitweight is 15-20 g. The fruit is bright red, round, and tastes verywell. It has excellent characteristics such as high lycopene content, long2storage. Its yield is 45.0 - 52.5 t·hm-2. It is high resistant to virus disease and fusarium wilt disease, resistant to root2knot nematode disease, tolerant to bacterial wilt disease. It is suitable for growing in open fields, p rotected fields in north of China.
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  • A New Asparagus Cultivar ‘WF28’
  • ZHANG Yuan-guo;LI Fang;LI Shu-hua;BAO Yan-cun;and LI Xia
  • Acta Horticulturae Sinica. 2009, 36(2): 309-309.
  • Abstract ( 2043 ) HTML ( 1264 ) PDF (93KB) ( 1264 )    
  • ‘WF28’ is a new all-male asparagus cultivar which derived from the cross of super2male plant‘SY2023’×female p lant‘NX1021’. Its growth period is 245 d. Its average stem height is about 220 cm. It has a series of good characteristics: Such as straightwhite asparagus, uniform size, close stem tip, no hollow stem, single shoot about 51 g, first2class shoot 9212% and iron content 0.043 mg·g-1. The average yield is 15 000 kg·hm-2. It is suitable to be cultivated forwhite or green asparagus.
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