Effects of Foliar Spraying of Nano-selenium on Amino Acid Content and Metabolome of Kiwifruit

doi:10.16420/j.issn.0513-353x.2024-0541

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (6): 1575-1587.doi: 10.16420/j.issn.0513-353x.2024-0541

• Cultivation?Physiology & Biochemistry • Previous Articles Next Articles

Effects of Foliar Spraying of Nano-selenium on Amino Acid Content and Metabolome of Kiwifruit

LIU Yanan¹, BAO Dandan¹, ZHANG Sipu², NIU Jiajia², XU Zhifei³, YANG Yongfeng³, and LU Yunfeng¹^,^*()

¹ School of Life Science and Agricultural Engineering，Nanyang Normal University，Nanyang，Henan 473061，China
² Institute of Horticulture，Henan Academy of Agricultural Sciences，Zhengzhou 450002，China
³ Henan Funiushan Biotechnology Co.，Ltd.，Nanyang，Henan 474350，China

Received:2024-08-08 Revised:2025-04-07 Online:2025-06-20 Published:2025-06-20
Contact: and LU Yunfeng

Abstract

Abstract:

To explore the effects and mechanisms of nano-selenium on the amino acid nutritional components of kiwifruit，‘Xuxiang’kiwifruit was used as the experimental material，with foliar spraying of nano-selenium at concentrations of 0，10，15，20，and 25 mg · L^-1. The changes in amino acid content were measured，and metabolomics sequencing of kiwifruit was performed. The results showed that after foliar spraying with 15 mg · L^-1 nano-selenium，the content of threonine，valine，isoleucine，leucine，phenylalanine，and other amino acids significantly increased，with a higher proportion of umami amino acids. A non-targeted metabolomics analysis identified 12 861 metabolites. Through KEGG and HMDB databases，the biosynthesis and metabolism of lipids and lipophilic molecules，phenylpropanoids，polyketides，and organic oxygen compounds in mature kiwifruit were further analyzed. The metabolites were mainly enriched in metabolic pathways related to amino acid metabolism，secondary metabolites，terpenoid compounds，and polyketide metabolism. Nano-selenium may increase the amino acid content in kiwifruit by stimulating secondary metabolic levels.

Key words: kiwifruit, nano-selenium, amino acids, nutritional evaluation, metabolomics

LIU Yanan, BAO Dandan, ZHANG Sipu, NIU Jiajia, XU Zhifei, YANG Yongfeng, and LU Yunfeng. Effects of Foliar Spraying of Nano-selenium on Amino Acid Content and Metabolome of Kiwifruit[J]. Acta Horticulturae Sinica, 2025, 52(6): 1575-1587.

Figures/Tables 11

References 34

[1]	Chen Hongjing, Yang Lijun, Song Tao. 2020. Amino acid composition analysis and nutritional evaluation of vegetables from Fujian Province. Journal of Hygiene Research, 49 (6):978-983. (in Chinese)
	陈宏靖, 阳丽君, 宋涛. 2020. 闽产25种蔬菜氨基酸含量及营养评价. 卫生研究, 49 (6):978-983.
[2]	D'amato R, Fontanella M C, Falcinelili B, Beone G M, Bravi E, Marconi O, Benincasa P, Businelli D. 2018. Selenium biofortification in rice(Oryza sativa L.) sprouting:effects on Se yield and nutritional traits with a focus on phenolic acid profile. Journal of Agricultural Food Chemistry, 66 (16):4082-4090.
[3]	D'amato R, Regni L, Falcinelli B, Mattioli S, Benincasa P, Bosco A D, Pacheco P, Proietti P, Troni E, Santi C, Businelli D. 2020. Current knowledge on Selenium biofortification to improve the nutraceutical profile of food:a comprehensive review. Journal of Agricultural Food Chemistry, 68 (14):4075-97.
[4]	El-ramady H R, Domokos-szabolcsy É, Abdalla N A, Alshaal T A, Shalaby T A, Sztrik A, Prokisch J, Fári M. 2014. Selenium and nano-selenium in agroecosystems. Environmental Chemistry Letters, 12 (4):495-510.
[5]	Feng Liangrui, Du Huiling, Wang Yuexin, 2007. Effects of foliar spraying of selenium on selenium content,yield,and quality of lettuce. Journal of Shanxi Agricultural University,(3):291-294. (in Chinese)
	冯两蕊, 杜慧玲, 王曰鑫. 2007. 叶面喷施硒对生菜富硒量及产量与品质的影响. 山西农业大学学报,(3):291-294.
[6]	Gao X, Liu E, Yin Y, Yang L, Huang Q, Chen S, Ho C T. 2020. Enhancing activities of salt-tolerant proteases secreted by Aspergillus oryzae using atmospheric and room temperature plasma mutagenesis. Journal of Agricultural Food Chemistry, 68 (9):2757-2764.
[7]	GB/T 5009.124-2016. Determination of amino acids in food safety national standards. (in Chinese)
	GB/T 5009.124-2016. 食品安全国家标准食品中氨基酸的测定.
[8]	Guo Lina, Wang Lu, Hao Xinyuan, Qi Meng, Li Xiaoman, Wang Xinchao, Zeng Jianming. 2022. Physiological characteristics of selenium uptake in tea plant root. Acta Horticulturae Sinica, 49 (9):1967-1976. (in Chinese)
	郭丽娜, 王璐, 郝心愿, 祁蒙, 李晓嫚, 王新超, 曾建明. 2022. 茶树根系吸收硒的生理特性研究. 园艺学报, 49 (9):1967-1976.
[9]	Hu Wanxing, Shi Yu, Cheng Yuqi, Zhao Bosi, Zhou Yunyun, Zhang Yi. 2020. Effects of nano-selenium on the growth and its mineral element contents and quality characteristics of purple potatoes. Acta Botanica Boreali-Occidentalia Sinica, 40 (2):296-303. (in Chinese)
	胡万行, 石玉, 程玉琦, 赵博思, 周云云, 张毅. 2020. 纳米硒对紫色马铃薯生长及其矿质元素含量和品质特性的影响. 西北植物学报, 40 (2):296-303.
[10]	Huang X, Tang Q, Chen C, Li Q, Lin H, Bai S, Zhao J, Li J, Wang K, Zhu M. 2023. Combined analysis of transcriptome and metabolome provides insights into nano-selenium foliar applications to improve summer tea quality(Camellia sinensis). LWT-Food Science and Technology,175:114496.
[11]	Kong Qinghua. 2020. Physiological and biochemical effects of applying bio nano selenium to foxtail millet and its impact on yield[M. D. Dissertation]. Jinan: Shandong Normal University. (in Chinese)
	孔清华. 2020. 谷子施用生物纳米硒的生理生化效应及对产量的影响[硕士论文]. 济南: 山东师范大学.
[12]	Li D, An Q, Wu Y, Li J Q, Pan C. 2020a. Foliar application of selenium nanoparticles on celery stimulates several nutrient component levels by regulating the α-linolenic acid pathway. ACS Sustainable Chemistry & Engineering, 8 (28):10502-10510.
[13]	Li D, Zhou C, Li J Q, Dong Q, Miao P, Lin Y, Cheng H, Wang Y, Luo L, Pan C. 2022. Metabolomic analysis on the mechanism of nano-selenium alleviating cadmium stress and improving the pepper nutritional value. J Nanobiotechnology, 20 (1):523.
[14]	Li D, Zhou C, Zhang J, An Q, Wu Y, Li J Q, Pan C. 2020b. Nano-selenium foliar applications enhance the nutrient quality of pepper by activating the capsaicinoid synthetic pathway. Journal of Agricultural and Food Chemistry, 68 (37):9888-9895.
[15]	Li D, Zhou C, Zou N, Wu Y, Zhang J, An Q, Li J-Q, Pan C. 2021. Nanoselenium foliar application enhances biosynthesis of tea leaves in metabolic cycles and associated responsive pathways. Environmental Pollution,273:116503.
[16]	Li S R, Wang L, Ni S J, Wang H H, Liu X F, Liu C X. 2017. The amino acids content of different part of Stropharia rugoso-annulata and their nutrition evaluation. Food Research Development, 38 (8):95-99.
[17]	Li Xiuqi, Yin Guohong, Hao Haohao, Jia Baohua, Niu Xiaopei. 2019. Research progress on the absorption and utilization of selenium by plants and the biological enhancement of selenium in major crops. Gansu Agricultural Science and Technology,(4):65-71. (in Chinese)
	李秀启, 尹国红, 郝浩浩, 贾宝华, 牛小沛. 2019. 植物对硒的吸收利用及主要农作物硒生物强化研究进展. 甘肃农业科技,(4):65-71.
[18]	Liu K, Li S, Han J, Zeng X, Ling M, Mao J, Li Y, Jiang J. 2021. Effect of selenium on tea(Camellia sinensis)under low temperature:Changes in physiological and biochemical responses and quality. Environmental and Experimental Botany,188:104475.
[19]	Lu Pengfei, Gao Zhiqiang, Sun Min, Dong Shifeng, Ren Aixia, Hou Feifan, Yin Xuebin. 2020. Effects of exogenous selenium fertilizer on grain yield and selenium accumulation in wheat. Journal of Hebei Agricultural University, 43 (3):17-22. (in Chinese)
	卢鹏飞, 高志强, 孙敏, 董石峰, 任爱霞, 候非凡, 尹雪斌. 2020. 外源硒肥对小麦籽粒产量及植株硒元素积累的影响. 河北农业大学学报, 43 (3):17-22.
[20]	Mohtashami R, Movahhedi Dehnavi M, Balouchi H, Faraji H. 2020. Improving yield,oil content and water productivity of dryland canola by supplementary irrigation and selenium spraying. Agricultural Water Management,232:106046.
[21]	OSER B L. 1951. Method for integrating essential amino acid content in the nutritional evaluation of protein. Journal of the American Dietetic Association, 27 (5):396-402.
[22]	Sun Juanjuan, A Lamusi, Zhao Jinmei, Xue Yanlin, Yu Linqing, Yu Zhu, Zhang Yingjun. 2019. Analysis of amino acid composition and six native alfalfa cultivars. Scientia Agricultura Sinica, 52 (13):2359-2367. (in Chinese)
	孙娟娟, 阿拉木斯, 赵金梅, 薛艳林, 于林清, 玉柱, 张英俊. 2019. 6个紫花苜蓿品种氨基酸组成分析及营养价值评价. 中国农业科学, 52 (13):2359-2367.
[23]	Sun Pengbo, Wang Zhijun, Gegentu, Yuan Ning, Sun Lin, Si Qiang, Zhang Jiawei, Liu Yichao, Jia Yushan. 2023. Effects of spraying nano selenium on yield,nutritional quality,and selenium content of alfalfa. Chinese Journal of Grassland Science, 45 (8):79-87. (in Chinese)
	孙鹏波, 王志军, 格根图, 袁宁, 孙林, 司强, 张佳伟, 刘逸超, 贾玉山. 2023. 喷施纳米硒对紫花苜蓿产量、营养品质和硒含量的影响. 中国草地学报, 45 (8):79-87.
[24]	Tian M, Xu X, Liu F, Fan X, Pan S. 2018. Untargeted metabolomics reveals predominant alterations in primary metabolites of broccoli sprouts in response to pre-harvest selenium treatment. Food Research International,111:205-211.
[25]	Wang Qinghua, Jing Dawei, Du Zhenyu, Ma Hailin, Ma Bingyao, Liu Fangchun, Wang Jing. 2020. Effects of foliar spraying of selenium on selenium content and quality of winter jujube at different stages. Journal of Agricultural Resources and Environment, 37 (2):226-232. (in Chinese)
	王清华, 井大炜, 杜振宇, 马海林, 马丙尧, 刘方春, 王静. 2020. 不同时期叶面喷硒对冬枣含硒量与品质的影响. 农业资源与环境学报, 37 (2):226-232.
[26]	Wu Q, Wang J W, Tian Y X, Zhou C Y, Mao S X, Wu Q Y, Huang K. 2024a. Selenocysteine methyltransferase SMT catalyzed the synthesis of Se-methylselenocysteine to regulate the accumulation of glucosinolates and sulforaphane in broccoli. Horticultural Plant Journal, 10 (5):1151-1164.
[27]	Wu Q Y, Wu Q, Tian Y X, Zhou C Y, Mao S X, Wang J W, Huang K. 2024b. BoMyrosinase plays an essential role in sulforaphane accumulation in response to selenite treatment in broccoli. Horticultural Plant Journal, 10 (2):488-502.
[28]	Xiong Y, Yan P, Du K, Li M, Xie Y, Gao P. 2020. Nutritional component analyses of kiwifruit in different development stages by metabonomic and transcriptomic approaches. Journal of the Science of Food Agriculture,DOI:10.1002/jsfa.10251.
[29]	Yang Sandong. 2005. Studies on the physiology and biochemistry of selenium in red clover and its enrichment pattern[M. D. Dissertation]. Changsha: Hunan Agricultural University. (in Chinese)
	杨三东. 2005. 红三叶草中硒的生理生化及其富集规律研究[硕士论文]. 长沙: 湖南农业大学.
[30]	Yu H, Miao P, Li D, Wu Y, Zhou C, Pan C. 2023. Improving red pitaya fruit quality by nano-selenium biofortification to enhance phenylpropanoid and betalain biosynthesis. Ecotoxicol Environ Saf,267:115653.
[31]	Zhao Y, Hu C, Wang X, Qing X, Wang P, Zhang Y, Zhang X, Zhao X. 2019. Selenium alleviated chromium stress in Chinese cabbage(Brassica campestris L. ssp. pekinensis)by regulating root morphology and metal element uptake. Ecotoxicology and Environmental Safety,173:314-321.
[32]	Zhou C, Zhang J, Wu Y, Cheng H, Pang Q, Xiao Y, Li D, Pan C. 2022. Metabolomic analysis on the mechanism of nano-selenium biofortification improving the Siraitia grosvenorii nutritional and health value. Foods,DOI:10.1186/s12951-022-01739-5.
[33]	Zhu L, Wang P, Zhang W, Hui F, Chen X. 2017. Effects of selenium application on nutrient uptake and nutritional quality of Codonopsis lanceolata. Scientia Horticulturae,225:574-580.
[34]	Zhu Z, Zhang Y, Liu J, Chen Y, Zhang X. 2018. Exploring the effects of selenium treatment on the nutritional quality of tomato fruit. Food Chemistry,252:9-15.

纳米硒/（mg · L^-1） Nano-selenium	苏氨酸Thr	缬氨酸Val	蛋氨酸Met	异亮氨酸Ile	亮氨酸Leu	苯丙氨酸Phe	赖氨酸Lys
0（对照Control）	0.53 ± 0.01 b	0.51 ± 0 b	0.14 ± 0.01 ab	0.47 ± 0.02 b	0.54 ± 0.01 b	0.42 ± 0 b	0.61 ± 0.01 b
10	0.48 ± 0.02 c	0.48 ± 0.01 b	0.13 ± 0.01 bc	0.44 ± 0 c	0.50 ± 0 d	0.39 ± 0 c	0.55 ± 0.01 d
15	0.55 ± 0 a	0.59 ± 0.03 a	0.15 ± 0 a	0.52 ± 0.02 a	0.60 ± 0.01 a	0.47 ± 0 a	0.67 ± 0.02 a
20	0.52 ± 0.01 b	0.50 ± 0 b	0.14 ± 0 ab	0.46 ± 0 bc	0.52 ± 0.01 c	0.42 ± 0.02 b	0.58 ± 0.01 c
25	0.43 ± 0.01 d	0.44 ± 0 c	0.12 ± 0 c	0.40 ± 0 d	0.45 ± 0.01 e	0.36 ± 0.01 d	0.50 ± 0 e
平均Average	0.50	0.50	0.14	0.46	0.52	0.41	0.58

纳米硒/（mg · L^-1） Nano-selenium	苏氨酸Thr	缬氨酸Val	蛋氨酸Met	异亮氨酸Ile	亮氨酸Leu	苯丙氨酸Phe	赖氨酸Lys
0（对照Control）	0.53 ± 0.01 b	0.51 ± 0 b	0.14 ± 0.01 ab	0.47 ± 0.02 b	0.54 ± 0.01 b	0.42 ± 0 b	0.61 ± 0.01 b
10	0.48 ± 0.02 c	0.48 ± 0.01 b	0.13 ± 0.01 bc	0.44 ± 0 c	0.50 ± 0 d	0.39 ± 0 c	0.55 ± 0.01 d
15	0.55 ± 0 a	0.59 ± 0.03 a	0.15 ± 0 a	0.52 ± 0.02 a	0.60 ± 0.01 a	0.47 ± 0 a	0.67 ± 0.02 a
20	0.52 ± 0.01 b	0.50 ± 0 b	0.14 ± 0 ab	0.46 ± 0 bc	0.52 ± 0.01 c	0.42 ± 0.02 b	0.58 ± 0.01 c
25	0.43 ± 0.01 d	0.44 ± 0 c	0.12 ± 0 c	0.40 ± 0 d	0.45 ± 0.01 e	0.36 ± 0.01 d	0.50 ± 0 e
平均Average	0.50	0.50	0.14	0.46	0.52	0.41	0.58

纳米硒/ （mg · L^-1） Nano-selenium	天冬氨酸 Asp	丝氨酸 Ser	谷氨酸 Glu	甘氨酸 Gly	丙氨酸 Ala	酪氨酸 Tyr	组氨酸 His	精氨酸 Arg	脯氨酸 Pro
0（对照Control）	1.03 ± 0.02 b	0.46 ± 0.01 ab	1.70 ± 0.01 b	0.53 ± 0 b	0.46 ± 0 b	0.36 ± 0 b	0.23 ± 0 ab	0.55 ± 0 b	0.37 ± 0.01 b
10	0.98 ± 0.02 c	0.42 ± 0.03 c	1.63 ± 0.03 c	0.48 ± 0.01 c	0.41 ± 0.02 d	0.33 ± 0.01 c	0.22 ± 0.01 bc	0.62 ± 0.10 b	0.33 ± 0.01 d
15	1.12 ± 0.03 a	0.49 ± 0 a	1.94 ± 0.03 a	0.56 ± 0.01 a	0.48 ± 0.01 a	0.40 ± 0.01 a	0.26 ± 0.01 a	0.85 ± 0.02 a	0.38 ± 0.01 a
20	1.02 ± 0.03 bc	0.45 ± 0 bc	1.63 ± 0 c	0.52 ± 0.01 b	0.43 ± 0 c	0.36 ± 0.01 b	0.24 ± 0 ab	0.54 ± 0 bc	0.35 ± 0 c
25	0.86 ± 0.01 d	0.38 ± 0.03 d	1.40 ± 0 d	0.44 ± 0.04 d	0.38 ± 0 e	0.30 ± 0.02 d	0.20 ± 0.03 c	0.45 ± 0.01 c	0.30 ± 0 e
平均Average	1.00	0.44	1.66	0.51	0.43	0.35	0.23	0.60	0.35

纳米硒/ （mg · L^-1） Nano-selenium	天冬氨酸 Asp	丝氨酸 Ser	谷氨酸 Glu	甘氨酸 Gly	丙氨酸 Ala	酪氨酸 Tyr	组氨酸 His	精氨酸 Arg	脯氨酸 Pro
0（对照Control）	1.03 ± 0.02 b	0.46 ± 0.01 ab	1.70 ± 0.01 b	0.53 ± 0 b	0.46 ± 0 b	0.36 ± 0 b	0.23 ± 0 ab	0.55 ± 0 b	0.37 ± 0.01 b
10	0.98 ± 0.02 c	0.42 ± 0.03 c	1.63 ± 0.03 c	0.48 ± 0.01 c	0.41 ± 0.02 d	0.33 ± 0.01 c	0.22 ± 0.01 bc	0.62 ± 0.10 b	0.33 ± 0.01 d
15	1.12 ± 0.03 a	0.49 ± 0 a	1.94 ± 0.03 a	0.56 ± 0.01 a	0.48 ± 0.01 a	0.40 ± 0.01 a	0.26 ± 0.01 a	0.85 ± 0.02 a	0.38 ± 0.01 a
20	1.02 ± 0.03 bc	0.45 ± 0 bc	1.63 ± 0 c	0.52 ± 0.01 b	0.43 ± 0 c	0.36 ± 0.01 b	0.24 ± 0 ab	0.54 ± 0 bc	0.35 ± 0 c
25	0.86 ± 0.01 d	0.38 ± 0.03 d	1.40 ± 0 d	0.44 ± 0.04 d	0.38 ± 0 e	0.30 ± 0.02 d	0.20 ± 0.03 c	0.45 ± 0.01 c	0.30 ± 0 e
平均Average	1.00	0.44	1.66	0.51	0.43	0.35	0.23	0.60	0.35

纳米硒/（mg · L^-1） Nano-selenium	氨基酸总量（T） Total amino acid	必需氨基酸（E） Essential amino acid	非必需氨基酸（N） Non-essential amino acid	E/N%	E/T%
0（对照Control）	8.91 ± 0.02 b	3.23	5.69	56.70	36.18
10	8.40 ± 0.08 d	2.97	5.43	54.76	35.38
15	10.03 ± 0.07 a	3.55	6.49	54.68	35.35
20	8.70 ± 0.01 c	3.15	5.55	56.68	36.18
25	7.41 ± 0.04 e	2.70	4.71	57.30	36.43
平均Average	8.69	3.12	5.57	56.02	35.90

Effects of Foliar Spraying of Nano-selenium on Amino Acid Content and Metabolome of Kiwifruit

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纳米硒/ （mg · L^-1） Nano-selenium	评价指标 Evaluation index	苏氨酸 Thr	缬氨酸Val	蛋氨酸 Met	异亮氨酸 Ile	亮氨酸Leu	苯丙氨酸 + 酪氨酸 Phe + Tyr	赖氨酸Lys	必需氨基酸比值系数分值SRC	必需氨基酸指数 EAAI
0（对照 Control）	RAA	1.47	1.14	0.45	1.33	0.87	1.35	1.25	47.70	1.27
0（对照 Control）	RC	1.31	1.02	0.40	1.18	0.78	1.20	1.11	47.70	1.27
10	RAA	1.43	1.14	0.44	1.32	0.85	1.32	1.20	44.64	1.26
10	RC	1.30	1.04	0.40	1.20	0.77	1.20	1.09	44.64	1.26
15	RAA	1.38	1.17	0.42	1.30	0.85	1.34	1.22	53.60	1.29
15	RC	1.26	1.06	0.39	1.19	0.77	1.22	1.19	53.60	1.29
20	RAA	1.50	1.15	0.46	1.32	0.86	1.38	1.21	45.95	1.27
20	RC	1.33	1.02	0.41	1.18	0.76	1.23	1.08	45.95	1.27
25	RAA	1.45	1.18	0.47	1.35	0.87	1.37	1.22	38.48	1.24
25	RC	1.28	1.05	0.41	1.19	0.77	1.21	1.08	38.48	1.24

类别 Classification	氨基酸 Amino acid	味觉阈值 Taste threshold	不同浓度（mg · L^-1）处理的RCT RCT of different treatments					平均 Average
类别 Classification	氨基酸 Amino acid	味觉阈值 Taste threshold	0	10	15	20	25	平均 Average
甜味氨基酸 Sweet amino acids	甘氨酸Gly	1.1	0.48	0.44	0.51	0.47	0.40	0.46
	丙氨酸Ala	6.0	0.76	0.68	0.80	0.72	0.63	0.72
	苏氨酸Asp	2.6	0.20	0.18	0.21	0.20	0.17	0.19
	丝氨酸Ser	1.5	0.31	0.28	0.33	0.30	0.25	0.29
	脯氨酸Pro	3.0	0.12	0.11	0.13	0.12	0.10	0.12
	组氨酸His	2.0	1.14	1.11	1.28	1.20	0.98	1.14
鲜味氨基酸 Delicate amino acids	天冬氨酸Asp	0.03	34.47	32.63	37.27	34.07	28.72	33.43
	谷氨酸Glu	0.05	34.01	32.69	38.76	32.67	27.97	33.22
	赖氨酸Lys	0.5	1.23	1.11	1.34	1.16	1.00	1.17
苦味氨基酸 Bitter amino acids	缬氨酸Val	1.5	0.34	0.32	0.39	0.33	0.29	0.34
	蛋氨酸Met	3.0	0.05	0.43	0.50	0.47	0.40	0.45
	异亮氨酸lle	0.9	0.53	0.49	0.58	0.51	0.45	0.51
	亮氨酸Leu	3.8	0.14	0.13	0.16	0.14	0.12	0.14
	精氨酸Arg	0.1	0.55	6.19	8.52	5.41	4.54	6.03
芳香族氨基酸 Aromatic amino acids	苯丙氨酸Phe	1.5	0.28	0.26	0.31	0.28	0.24	0.27
芳香族氨基酸 Aromatic amino acids	酪氨酸Tyr	2.6	0.14	0.13	0.16	0.14	0.12	0.14

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