研究论文

• Investigation on Parasitic Nematodes Associated with the W ild Grapes Native to China
• Luo Sulan;He Puehao;Zhansun Dongting
• Acta Horticulturae Sinica. 2003, 30(02): 127-130.
• Abstract ( 1672 ) HTML ( 1416 ) PDF (171KB) ( 1416 ) 　　　

• Species and densities of parasitic nematode8 were systematically investigated from the daizosphere soil of 73 clone 8 which belong to 17 species of wild grapes native to China. 19 species of plant nematodes belonging to 9 genera respectively were identified from 300 soil samples，which contained six species of Helicotylenchus，four species of Xiphinema， two species of Paratylenchus， two species of Tylenchorhynchus， and one species of Longidorus, Paratylenchus， Rotylenchus， Criconemoides and Hoplolaimus each. Nematode population usually consisted of 2—5 genera．Three genera i．e Helicotylenchus, Paratylenchus and Tylenchorhynchus spread widely in many wild clones．Differences of nematode genera and densities among diferent wild clones were given．The results would be basis for stock selection resistant to parasitic nematodes．
  

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• The Evolution and Classification of Variety of Kyoho Grapevine Serie
• Li Huaifu
• Acta Horticulturae Sinica. 2003, 30(02): 131-134.
• Abstract ( 1596 ) HTML ( 1951 ) PDF (141KB) ( 1951 ) 　　　
• Through the introduction of Kyoho grapevine series and the observation recordation of characteristic and the analysis of the features of parents， the evolution process an d the methods of classification have been studied．The results indicates：(1)The evolution chart of Kyoho grapevine series worked out by the writer can systemly reflect the genetic relationship and the evolution process among the varieties of Kyoho grapevine series and the
  context；(2)The main way is cross—breeding，the next place is selection of bud variation and seedling selection；(3)The variety of Kyoho grapevine series can be classified by the origination，mature phase，the tinct of peel
  while ma turing and having seeds or not．
  
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• Studies on Abnormal Meiosis Producing 2n Pollens in Nonastringent Persimmon (Diospyros Kaki Thunb.)
• Gu Xiaofeng;Luo Zhengrong;
• Acta Horticulturae Sinica. 2003, 30(02): 135-140.
• Abstract ( 1565 ) HTML ( 1257 ) PDF (280KB) ( 1257 ) 　　　
• The objective of this study was to determine the eytolngieal origin of 2n posen in ‘Zenjiman’ persimmon．Pollen mother cel analyses revealed that 2n polen resulted from fused spindles，triange spindles，or abnormal disorientation of sister ehromatid during metaphase II and anaphase II of mierosporogenesis．94.8% of 2n po len formation was controled by fused spindles and triange spindles ，which were genetically equivalent to first division restitution(FDR)type of gamete formation．The other 2n polens were genetically equivalent to second division restitution (SDR)type of gam ete formation．Thus 2n polen produced from IjiIIlal1l persimmon call provide potential value for po lyploidy breeding in persimmon．
  Key words：
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• Salicylic Acid Alleviated the Damage Caused by Low Temperature to Cell Merebrane and Some Photosynthetic Functions of Banana SeeaUngs
• Kang Guozhangt;Ou Zhiyingt;Wang Zhengxun;Sun Guchou)
• Acta Horticulturae Sinica. 2003, 30(02): 141-146.
• Abstract ( 1653 ) HTML ( 1516 ) PDF (263KB) ( 1516 ) 　　　
• The possible physiological mechanism of enhancement of cold tolerance by salicylic acid(SA)in banana seedlings(Musa A L．CV．Williams 8188)was explored．1 d of 0.3—0.9 mmol·L^-1 SA treatment at 22/15 ℃ by spayed to leaves and irrigated to roots of banana seedlings)significantly enhanced chilhng-resistance of banana seedlings during subsequent 7 ℃ or 5 ℃ low temperature stress， indicated by the reduction of electrolyte leakage or wilting aIea，respectively．Under normal growth condition(22/15 ℃)，1 d of0．5 mmol·L^-1 treatment eaused a slight decrease of Fv/Fm，Fv’/Fm’，qP，卯S1I and Pn．However，SA pretreatment markedly inhibited the reduction of Fv/Fm, Fv’/Fm’,qP，卯SⅡ and Pn，and alleviated the rapid increase of Fo and NPQ during 3 d of 7 ℃ low temperature．And during 2 d of recovery periods(22/15%：)，SA pretreattment also accelerated the recovery of these photosynthetic parameters above．Th e results indicated that SA pretreatment could provide a protection for the cell membranes and the photosynthetic functions of the cold-stIIessed banana see(uirlgs， which could help to enhance thechiling tolerance of banana plants．
  
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• Efect of Postharvest Treatment with GA₃ on Physiological and Biochemio!Changes of‘Cui jujube’Fruit during Cold Storage
• Xue Menglin;Zhang Ping;Zhang Jishu;Wan g Li
• Acta Horticulturae Sinica. 2003, 30(02): 147-151.
• Abstract ( 1493 ) HTML ( 1425 ) PDF (208KB) ( 1425 ) 　　　
• In this experiment efect of postharvest treatment with GAa on physiological and biochemical changes of‘Cui jujube’fruit during cold storage was studied．The results showed that the treatment significantly delayed the decrease of jujube fruit firmness，inhibited the increase in contents of acetaldehyde and ethanol and the activities of alcohol dehydrogenase and polyphenol oxidase，reduced respiratory intensity and rate of ethylene produetion，slowed down the occurence of alcoholism and flesh browning ．
  
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• Efects of Nutrient Solution of Different Concentrations on Growth Properties ofLactuca saliva var. Longifolia Lam．
• Fan Shuangxi
• Acta Horticulturae Sinica. 2003, 30(02): 152-156.
• Abstract ( 1318 ) HTML ( 1527 ) PDF (220KB) ( 1527 ) 　　　
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• The Temperature Compensation Point and the Start Time of Photosynthesis of Cucumber Leaves
• Zhang Zhenxian;Ai Xizhen;Zhao Shijie;Yang Hongyan
• Acta Horticulturae Sinica. 2003, 30(02): 157-162.
• Abstract ( 2334 ) HTML ( 1353 ) PDF (240KB) ( 1353 ) 　　　
• Under controlling temperature and light intensity conditions ， the temperature compensation point (TCP)and the star time of photosynthesis(STP) of cucumber leaves have been studied by nsms a CIRAS-1 portable photosynthetic instruments which manufactured by PP system company of England．The results showed that the TCP of cucumber leaves 3.3℃ at low temperature(3—15℃ and 400 μmol·m ^-2·s PFD)and 48.9—50.7℃ at high temperature(40—48℃ and 1300 μmol·m ^-2·s PFD)respectively． efect of low temperature
  0n TCP in diferent cucumber varieties was smal1．TCP of CV.‘Xintai mici’，a solar greenhouse variety，was relativelv lower at high temperature than that of the ‘Jinyan 4’， an open-field variety． The TCP of cucumber of old
  leaf Was higher than that of young leaf under low temperature．Generally，the stal~time of photosynthesis(namely，Pn from zero to maximum) in cucumber functional leaf was 42—45 min，but that in old lear was 54—60 min．
  
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• Genetic Analysis of Resistance to Bacterial Wilt (Ralastonia solanac earum) inEggplant(Solanum melongena L．)
• Feng Linlin;Qu Dongyu;Jin Liping;Lian Yong
• Acta Horticulturae Sinica. 2003, 30(02): 163-166.
• Abstract ( 1773 ) HTML ( 1594 ) PDF (164KB) ( 1594 ) 　　　
• Six eggplant inbred lines with diferent resistance to bacterial wilt were used as parents in this ex—
  periment．The populations were evaluated for their reaction to bacterial wilt by artificial inoculation at the seedling
  stage．By using Grifing I an d Hayman methods，a 6×6 dialel analysis was done to analyze the variance of the
  combining ability and estimate the genetic parameters．It Was suggested that the inheritance of the resistance to bac—
  terial wilt in the eggplan t fit to the ‘additive—dominant’ model，additive efect，dominant efect an d reciprocal effect
  all p|ayed a role in the genetics of the resistance to bacterial wilt in eggplant，but the additive efect Was the
  major．The resistance to bacterial wilt in eggplant Was recessive and the susceptibility Was partial dominant．The re—
  sistance to bacterial wilt in eggplant Was controlled by a lot of minor genes，several major genes and cytoplasm
  genes ·
  Key words： Bacterial wi lt；Resistance；Genetic analysis； Eggplan t
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• Efects of Ozone on Growth and Yield of Chinese Cabbage
• Bai Yueming;Wang Chunyi;Liu Ling;Guo Jianping;Wen Min
• Acta Horticulturae Sinica. 2003, 30(02): 167-170.
• Abstract ( 1307 ) HTML ( 1086 ) PDF (177KB) ( 1086 ) 　　　
• This study was conducted to determine the efects of diferent 0₃ concentrations (A,8.93×10^-9mol/L;B,4.46 × 10^-9mol/L;C,≤ 4.46×10^-10 mol/L； Control， ambient 03 concentrations were about 1.12×10^-9—1.79×10^-9 mol/L)and diferent exposure last times( gatiIlg 5 d， 10 d，former 15 d，later 15 d and 30 d)at 8.93×10 mol/L oll Chinese cabbage by using 4 farmland open top chambers OTC-1，The results showed that yellow leaf rate increased．daily increase of total biomass decreased an d the ways of assimi．1ation products distribute to organs cha sed with 03 concentrations increase.Leaf number， leaf area， net photo．synthesis of the outer leaves and yield of Chinese cabbage were reduced greatly as the exposure last time at 8.93×10^-9mol/L was prolonged during experiment．And there was little efect to the other leaves. At the same 0₃ concentration(8.93×10^-9mol/L)and exposure last time during diferent growth time of Chinese cabbie．the responses of economy yield and total biomass to 0₃ concentration prophase treatment were nlOl~heavier than that of anaphase．Leaf al a was contrary．But there was no efect to leaf numbers．
  
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• Plant Resources of Genus Hypericum L．in Yunnan
• Li Jingxiu;Guan Kaiyun;Kong Fancai
• Acta Horticulturae Sinica. 2003, 30(02): 171-174.
• Abstract ( 1405 ) HTML ( 1242 ) PDF (190KB) ( 1242 ) 　　　
• Based on the field investigation and data processing on genus Hyperiaum L. in Yunnan, this paper has summarized and described the natural distribution characteristics and the ornamental character，state of resources of the 24 species．And it made suggestion that exploited an d made use of natural resources on genus Hyperiaum L. in Yunnan．
  
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• Heredity of Several Flower Characters in Dendranthema grandiflora witll SmallInflorescences
• Chen Fadi;Jiang Jiafu;Guo Weiming
• Acta Horticulturae Sinica. 2003, 30(02): 175-182.
• Abstract ( 1776 ) HTML ( 1213 ) PDF (252KB) ( 1213 ) 　　　
• An observation was made on some characteristics of flowers in F₁ generation by the 28 multiple combinations between 30 varieties of Chrysanthemum.The results indicated that red color of flowers was stronger than yellow at genetic， and original color of yellow and white didn’t be long to matroclinal heredity．White matroclinal could produce’vide separating color in F₁ generation．The inflorescence diameter． number of ray florets and tubular florets appeared heterosis in F₁ generation．The hybrid’s mean inflorescence diam eter， the number of ray florets an d tubular florets were 122.%， 128.% and 124.7% of parent’s respectively， and the value of these three characters inclined more slowly， even appeared hybrid weakness except formean infl orescence diameter as the parent’s increased．The heredity of flat plate shape was stronger than 1—3 cycle plate shaped flower．
  
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• Studies On Rapid in Vitro Propagation of Phaeomeria magnisqca
• Pan Xuefeng;Wang Cha
• Acta Horticulturae Sinica. 2003, 30(02): 183-186.
• Abstract ( 1418 ) HTML ( 1362 ) PDF (175KB) ( 1362 ) 　　　
• The research result of the in vitro propagation of the Phaeomeria magnifica is presented in this paper．The results showed that： Using MS+6-BA 2.0 mg/L+NAA 0.1 mg/L+CW 10% ills proliferation median3. An optimal proliferation rate Wills obtained，the proliferation rate could reach 4.3 times：Using double shoots ills explants，after 30 clays of culture in this median3， it could amplify 5.4 times． The root formation of Phaeomeria magnifica tissue culture shoots had a low demand for exotic hormones，even in a medium of no hormones it could still form a gooa root system， the rooting rate could reach 100%．The transplantation of Phaeomeria magnisqca tissue culture shoots wills simple and feasible．There wills no strict demand for matrix，when the planflets were transplanted to superficial soil，the survival rate could reach 100%．and the plants grew well．
  
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• M orphological Studies on Paeonia Sect. Moutan Subsect Vagiatae in China
• Yuan Tao;Wang Lianying
• Acta Horticulturae Sinica. 2003, 30(02): 187-191.
• Abstract ( 1693 ) HTML ( 1416 ) PDF (199KB) ( 1416 ) 　　　
• By investigating and specimen consulting and literatures searching, morphological characters and variations of diferent populations of every species，interspecies hybrids and a ginal cultivated type seedling‘Fengdanbai’ in Sect． Moutan Subsect Vagiatae were systematically observed and described． According to the results，the authors put forward some classificatory characters，genetic rules of purple blotches at the base of petals and color of flower disc an d filaments， and drew a conclusion that plentiful variations of cultivated tree-peony comeing from hybridizntion of wild species．Classificatory degree of Paeonia yananensis and P.rockii sp.linyanshanii were also discussed． Finally，morphological evolvement trends in the subsect were elementarily analysed.
  
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研究报告

• Efects of Twig Girtmng at! Different Stages on New Shoot Growth and CarbonNutrient Reservation
• Huang Xuming;Wang Huicong;Yuan Weiqun
• Acta Horticulturae Sinica. 2003, 30(02): 192-194.
• Abstract ( 1538 ) HTML ( 1287 ) PDF (121KB) ( 1287 ) 　　　
• Effects of twig girdling at different shoot stages in spring and autumm on new shot growth and starch accumulation in the twigs of litchi (Litchi chinenis Sonn．CV．Huaizhi)were studied．Girdling significantly inhibited the new shot growth， resulted in the formation of swelling callus above the girdle and
  significant thickening， and caused massive accumulation of starch in the twigs. The inhibitory efect on shot growth and stimulative effect on starch accumulation of girdling in autumn were significantly stronger than the treatment in spring．Girdling position，i．e．distance from girdle to base of new shot or apical bud(L) and the area of the old leaves above the girdle did not influence the inhibitory efect of giIdliIlg．However，there was a significant positive correlation between starch accum ulation and the ratio of S/L．i．e．leaf area in unit length of shot．The stage of new shot development exerted a significant influence on the inhibitory effect of saalms．The new shot hardly grew when girdling Was done before the bud elongated．while there was considerable new shot growth when girdling at rapid elongation stage．
  
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• Attenuation of Cbilliog Efect on Photosynthesis of Satsuma Mandarin(Citrus unshiu Marc．)by NaHSO₃ Application
• Guo Yanping;Su Jihu;Wang Hongwei;Shen Yungang;Zhang Liangcheng
• Acta Horticulturae Sinica. 2003, 30(02): 195-197.
• Abstract ( 1506 ) HTML ( 1284 ) PDF (145KB) ( 1284 ) 　　　
• (¹Depatment of Horticulture, Zhejiang University,Hangzhou 310029, China; ²Shanghai Institute of Plant Physiology and Ecology,Acdemic Sinica,Shanghai 200032,China )
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• The Relationship between Heat Induced Chilling Tolerance and the Changes inProtein Contents
• Zhu Shijiang;Ji Zuoliang;Zhang Zhaoqi;Lu Wangjin
• Acta Horticulturae Sinica. 2003, 30(02): 198-200.
• Abstract ( 1356 ) HTML ( 1078 ) PDF (133KB) ( 1078 ) 　　　
• This paper dealt with the relationship between heat induced chilling tolerance in ‘Zihua’(Mangifera indica L．) fruits and changes in protein contents，with results as follows：1)12 h to 3 d of hot-air treatment at 38 ℃ significantly enhanced the chilling tolerance of‘Zihua’ fruits． 2) The heat treatment increased the protein levels in mango fruits， and during low temperature storage at 2℃ and ripening at 20℃，the heated mangon remained hi in protein contents．3) Among the proteins increased as a result of heat treatment，the
  heat-stable proteins mango up a relatively high protein．These results showed that the heat．induced proteins were related to the chilling tolerance，and that the heat．stable proteins newly produced under heat stress was one of the most important factors in inducing chilling tolerance．
  
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• The Effect of High-voltage Static Electric Field and Spermidine on the ColorChange of‘Dongzao’Jujube
• Li Lite;Dan Yang;Wang Jie
• Acta Horticulturae Sinica. 2003, 30(02): 201-203.
• Abstract ( 1391 ) HTML ( 1293 ) PDF (128KB) ( 1293 ) 　　　
• We studied the color-changing regulation of‘Dongzao’jujube and evaluated several treatments such as high-voltage smile electric field (HVSEF)and spermidine．The results showed that each treatment has special efect on each index of CIELAB system．Th e result of chromatic aberration shows that spermidine is the best chemical to keep the color of ‘Dongzao’jujube and high—voltage smile electric field hardly can keep the color of ‘Dongzao’jujube．
  
  
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• A Comparative Study of RAPD and Morphological Approaches to CharacterizeRelationships of Rosaroxburghii Tratt．and Its Relatives
• Wen Xiaopeng;PangXlin;Deng Xiuxin
• Acta Horticulturae Sinica. 2003, 30(02): 204-206.
• Abstract ( 1562 ) HTML ( 1039 ) PDF (143KB) ( 1039 ) 　　　
• This sixty morphological traits and RAPD markers amplified with 29 arbitrary primer used to discriminate the accessition, and to evaluate the relatedness of Rosa roxburghii Tmtt．and a sample set of its relatives．Compared with the relatives，considerable close relationships were found among the genotypes of R．burghii Tmtt．Furthermore，a hish relatedness was also obtained between R．S．Shi and D. Shi and R． kweichowensis Yu et Ku．A Mantel correspondence test showed a significant correlation (r=0．6122，P<0．01)between morphological traits and markers，despite certain discontance existing in the two approaches． In addition，the origination of R．sters S．D． was discussed in the paper．
  
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• Regeneration and Antibacterial Peptide Gene Transformation of Cherry Lealves
• Wang Guanlin;Xia Xiuying;Zhong Wentian;Fang Hongjun;Jiang Minglan
• Acta Horticulturae Sinica. 2003, 30(02): 209-211.
• Abstract ( 1411 ) HTML ( 1060 ) PDF (149KB) ( 1060 ) 　　　
• The leaf regeneration and Agrobacterium tumefaciens—mediated transformation system of cherry rootstock 98-1，Colt and Dagingye were established at the first time．The results showed that the best medium Ms supplemented with 1.0—2.0 mg/L BA，0.3—0.5 mg/L NAA，0.5 mg/L GA and 5.0—10.0 mg/L AgNO₃ promoting leaf regeneration frequency.ephysiological reaction of Agrobacterium tumefaciens and explant cell were key to transformation．Delay selection could improve resistance to Kan of transformed cell in leaves and do wel to regenemtion．Results of molecular biological assays with PCR an d Southern blot hybridization preliminarily indicated that the an tibacterial peptide genes had been integrated into the genome of cherry．
  
  
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• Analysis on Physiological and Biochemical Characteristics between Male Sterile and Fertile Plants of Genic Male Sterile AB Line in Chinese Cabbage
• Wang Yongqin;Cao Jiashu;Yu Huifang;Ye Wanzhi;Yu Xiaolin;Xiang Xun;Lu Gang
• Acta Horticulturae Sinica. 2003, 30(02): 212-214.
• Abstract ( 1363 ) HTML ( 1137 ) PDF (144KB) ( 1137 ) 　　　
• The male sterile plants were significan tly higher than male fertile plants during rosette and anthesis in recessive genic male sterile AB line in Chinese cabbage (Brassica campestris L．ssp． chinensis Makino)． Cyanide-ilsistant respiratory rate of buds in male fertile plants were significantly higher than that in male sterile plants．Buds of male sterile plants had deficient IAA an d surplus ZT．The bands of peroxidase isozyme of middle and big buds in male fertile plants were moil obvious than in male fertile plants．There were two tapes of cytochrome oxidase isozyme of little and mi ddle buds and moil obvious in male fertile plants．There were two specific tapes in soluble protein electrophoilsis in male fertile plan ts，and one in male sterile plants．
  
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• Changes of Lipid Peroxidation and Activities of Protective Enzymes in Asparagus Bean seedlings under Phosphorus-deficiency Stress
• Liu Houcheng;Kuang Yanhu;Chen Riyuan
• Acta Horticulturae Sinica. 2003, 30(02): 215-217.
• Abstract ( 1479 ) HTML ( 1103 ) PDF (111KB) ( 1103 ) 　　　
• act：Changes of activities of protective enzymes superoxide dismutase(SOD)，catalase (0 )，peroxidase(PoD)] and MDA contents in leaf and root of asparagus bean [ Vigna unquiculata W． ssp． (L．) Verd．cultivars differing in sensitivity to phosphorus-deficiency under phosphorus-deficiency
  stress were studied． Under P deficiency stress, activities of protective enzymes in ‘Luhua bai’ increased or increased more，however that in ‘Erlu bai ’ decreased or increased less． Under P．deficiency P-activities of
  SOD and POD in asparagus bean seedling root increased， enhanced llgnification of root and vessel diferentiation, then enhanced root capability of translocation and absorption of water and nutrient， that of ‘hlhu8 bai’increased iilore．
  
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• Effects of Iodine Application on Growth and Content of Iodine， Amino Acid，Vitamin C an d Fibre in Radish Sprouts
• Xia Shitou;Peng Keqin;Xiao tangtao;Hu Zhimin
• Acta Horticulturae Sinica. 2003, 30(02): 218-220.
• Abstract ( 1500 ) HTML ( 1173 ) PDF (126KB) ( 1173 ) 　　　
• Effects of iodine with diferent adding manner8 on radish sprouts in culture solution was studied．The results showed that the growth of the root，at iodine concentration of 0.5—1.5 mg/L，was restrained ，while the content of vitamin C， total free amino acid and fibre content in edible parts of radish sprouts were increased．the concentration exceeded 1.5 mg/L, the beneficial efects of iodine diminished even changed into toxic elfects．The iodine content in edible parts of sprouts mised up significantly with the increasing of the concentration of iodine in culturing solution．There existed significant diferences among varieties and between iodine adding n'ifllmer．
  
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• Effects of Potassium Levels on Photosynthesis and Fruit Quality of Muskmelonin Medium Culture
• Lin Duo;Huang Danfeng
• Acta Horticulturae Sinica. 2003, 30(02): 221-223.
• Abstract ( 1271 ) HTML ( 1231 ) PDF (128KB) ( 1231 ) 　　　

• Experiments showed that net photosynthetic rate of leaves，the concentration of total sugar，freshness amino acid，sweetness amino acid，and acetate components(n-amy1 acetate，2-butoxyethyl acetate)of flesh were significantly increased in 24O mg·L^-1 potassium level， which resulted in improving photosyn thesis and quality of muskmelon．However，no significan t diference in yield was recorded among the treatments．Potassium imufllcient or excessive in this stuay resulted in increased non．stomatal linfirations to net photosyn thetic rate．Favorable nutrient value and flavor of muskmelon in medium culture were achieved when potassium level was adjusted to 240 mg·L^-1 in this study．
  

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• Effect of Plant Growth Regulators and Heat Shock on Shoot-tip Culture inBrassica campestris L．ssp． chinensis var． utilis Tsen et Lee．
• Liao Feixiong;Pan Ruichi;He Xiaoming
• Acta Horticulturae Sinica. 2003, 30(02): 224-226.
• Abstract ( 1345 ) HTML ( 999 ) PDF (137KB) ( 999 ) 　　　
• The propagation in vitro of shoot-tips and quality of plandets of Brassica campestris L．ssp． chinensis var． utilis Tsen et Lee．were affected by the combination of BA and NAA with diferent concentrations．Higher rate of shoot proliferation with better quality of planflets could be obtained when the concentration of BA in medium was mole than 0.8 mg·L^-1 and optimal concentration of NAA was less than 0.5 mg·L^-1．The efect of KT or ZT respectively on propagation WaS better than that of the combination of the both when total concentrations were identica1．The orientations of plant was found to afect the proliferation rate．Heat shock with shoot—tips at
  45 ℃ for 2 h could stimulate the proliferation of shoots．
  
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• Efficient Culture and Plan t Regeneration from Cotyledon Protoplasts of ‘Cuixiu’Cucmnber
• Guo Dezhang;Yan Zhen;Lai Zhongxiong;Lin Qingliang;and Wang Jiafu
• Acta Horticulturae Sinica. 2003, 30(02): 227-228.
• Abstract ( 1364 ) HTML ( 1079 ) PDF (94KB) ( 1079 ) 　　　
• Isolated and purified cotyledon protoplasts of cucumber(Cucummis sativust L． ‘Cuixiu’)were cultured at diferent population density ranging from 5.5×10/mL to 5.5×10⁵ /mL in mKM8p liquid medium respectively an d underwent cell division in varying degrees．Sustained cel divisions with eventual somatic colony production occurred only when the population density of protoplasts exceeded 1.0×10³/mL． the highest plating efficiency was about 54% ．Lager quantifies of embryoids diferentiated from protoplast derived ealli and then converted into planflets after succedent induction culture．Results demonstrate that the concentration of Ca²⁺ has an important effect on the stability an d cel division of cucumber protoplasts．
  
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• The Bulblet Morphogenesis of Lilium formolongi in Scale Propagation
• Ning Yunfen;Zhou Hougao;Huang Yuyuan;Wang Fenglan
• Acta Horticulturae Sinica. 2003, 30(02): 229-231.
• Abstract ( 1452 ) HTML ( 1464 ) PDF (167KB) ( 1464 ) 　　　

• The bulblet morphogenesis of Lilium formlongi in scale propagation is showed in present paper．The results showed that the bulblets originated from the inner parenehyma tissue of scale not callus tissue．The process of bulblet morphogenesis was divided to four stages．
  

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• Difference of Endopeptidase Activity in Petals between Two Cut Rose Cultivars with Diferent Tolerance to W ater Deficit Stress during Flower Opening and Senescence
• Cong Richen;Zhao Xiting;Liu Xiaohui;Gao Junping
• Acta Horticulturae Sinica. 2003, 30(02): 232-235.
• Abstract ( 1554 ) HTML ( 1105 ) PDF (178KB) ( 1105 ) 　　　
• Cut rose(Rosa hybris )‘Samantha’，a cultivar with strong tolerance to water deficit stress，and‘Belami’，a cultivarwith middle range tolerance weI'e used to investigate the cha ses of contents of soluble protein and free amino acid， and endopeptidase activity in petals during flower opening and senescence． The results showed that obvious differences between the two cultivars were obtained in contents of solub le protein and free amino acid，an dendopeptidase activity during flower ope ning and senescence，although the salTle change patterns were obtained ．Soluble protein content was lower in ‘Samantha’tllall in ‘Belami’．Endope ptidase activity Was much higher at pH 10，although it Was lower at pH 6 in‘Samantha’than in ‘Belami’．However，it Was confirmed in petals of the two cultivars that there were two kinds of endopeptidase：mercapto proteinase an d serine acid proteinase．The results above suggested that the diference of endopeptidase activity between two cultivars might be related to their tolerance to water de6c stress．
  
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• In Vitro Efficient Plant Regeneration th Platanus orientalis L． Leaves asExplants
• Fan Guoqiang;Jiang Jianping;He Yaoqing;Li Feng
• Acta Horticulturae Sinica. 2003, 30(02): 236-238.
• Abstract ( 1458 ) HTML ( 1208 ) PDF (114KB) ( 1208 ) 　　　
• This paper dealt with in vitro eficient o~ant regeneration on WPM media with 0.6(mg· L^-1，the same below)IBA and 8.0 BA，using Platanus orientalis L．1eaves and their calli as explanm．The results indicated that WPM might be the basic medium for the plant regeneration，and that bud inductionⅡ ia of the 5th，6th，7th leaves (from the bases of seedlings) +0.1 IBA+3.0—2.0 BA，and the 5th leaf cam induced on +0.6IBA+8.0 BA medium was +0.1 IBA+4.8 BA．Root induction medium was 1/2 MS+0.1—0.5IBA．These results might provide reference to cell and gene engineering manipulations and quick propagations of Phuanus orienitalis．
  
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• Rapid Propagation of Machilus yunnanensis var． duclouxii in Vitro
• Lin Ping;Pu Xiaolan
• Acta Horticulturae Sinica. 2003, 30(02): 239-241.
• Abstract ( 1542 ) HTML ( 1209 ) PDF (127KB) ( 1209 ) 　　　

• The culture of the stem-bud from crown and stump shoot of Machilus yunnanensis var． duclouxii in vitro was studied．The results showed that the less browning and high germination rate of axially bud from the stem-bud appeared on the medium of MS+BA 4mg·L^-1+NAA 0.5 mg·L^-1，and the plan tlets grew well，which was beneficial to later mutipulcation．Th e tufted buds were diferentiated on the mediunl of MS+BA 8mg·L^-1 +NAA 0.5 mg·L^-1+KT 1.0 mg·L^-1，but multiplication rate was low．On the medium of 1/2 MS + NAA 0.1 mg·L^-1，the white healthy roots were induced ．The taken parts，taken-time of explants of Machilus yunnanensis var． duclouxii and hormone in medium are main factors that cause browning an d afect the speed of propagation during breeding．
  
  

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• In Vitro Culture of the Inflorescence of Phalaenopsis
• Chen Zhilin;Ye Xiulin;Liang Chengye
• Acta Horticulturae Sinica. 2003, 30(02): 242-244.
• Abstract ( 1562 ) HTML ( 1302 ) PDF (133KB) ( 1302 ) 　　　
• Inflorescences were induced by culturing flower-stalk buds of Phalaenopsis on V&W medium containing vitamin component of B5 medium and 5.0 mg·L^-1 benzylaminopurine(BA) under 22°C．Adventitious shoot were induced from the intemodal segments of inflorescence by using the NS medium containing vitamin of B5 medium 0.5 mg·L^-1BA and 0. mg·L^-1 NAA．The low sucrose concentration benefits the shoot's diferentiation and growth． Shoot were propagated on the same medium and developed into plantlet on Hyponex 1 nlediuln containing 100 g·L^-1 banana juice and 2 g·L^-1 active charcoal．
  
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新品种

• New Earliest Apricot Six Cultivars from Embryo Culture
• Shi Yinping
• Acta Horticulturae Sinica. 2003, 30(02): 245-246.
• Abstract ( 2898 ) HTML ( 999 ) PDF (91KB) ( 999 ) 　　　
• In 1990， the six apricot cultivars were successfully cultured from embryo cutlure．The characteristics of Tube Hongguang 1，2，3 are large fruit and bright red color． Tube Zaohe 2 has excellent quality．Tube Taoxing 1’s fruit shape is similar to peach． Tube Tianfeng has sweet seeds．six new cultivars showed tlle simle characteristics with early ripening，early bearing，high yield，frost resistance，high economical value and widely development prospect．
  
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• ‘Yanchuan Goutouzao’一A New Variety of Zizyphus jujuba Mill.
• Li Xingang;Liu Yuzhong;Tong Jinxia;Huang Jian;Gao Wenhai
• Acta Horticulturae Sinica. 2003, 30(02): 247-247.
• Abstract ( 1580 ) HTML ( 950 ) PDF (55KB) ( 950 ) 　　　
• ‘Yanchuan Goutouzao’is a new fruit-drying variety of jujube．The fruit is large，oval or taper shape ，the average fruit mass is l8.7 g，the heaviest reaches 25.4 g．The fruit is unifonTl，attactive，good quality fruit，and applicable to fruit-eating and acing．The flesh is crisp，juicy，and sour sweet．The fresh fruits ripen in late September， the ripening date of drying -fruit is in early October in north Shaanxi． Th e fresh fruits have long storage life．And its cultivated region is in north China with the cool temperature(10.0—11.0℃)and rainfall(annual preciptafion of about 500 mm)．
  
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• ‘Jianghuangzhuang 1’一A New Dehiscent-fruit-resistant Strain of ‘Jinsixiaozao’Jujube
• Ran Xintuo;Wang Xuejun
• Acta Horticulturae Sinica. 2003, 30(02): 248-248.
• Abstract ( 1279 ) HTML ( 857 ) PDF (46KB) ( 857 ) 　　　
• ‘Jianghuangzhuang 1’is a new dehiscent-fruit-resistant strain of‘Jinsixiaozao’jujube with ellipse，high quality fruits．There are no dehiscent fruits due to the rains during mature period in this strain
  ， while it happened commonly in 8ome strains of‘Jinsixiaozao’jujube．The fruit commodity value of this strain are increased．
  
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• A New Hot Pepper Hybrid ‘Longjiao 2’
• Wang Lanlan;Cheng Hong;Chen Lingzhi
• Acta Horticulturae Sinica. 2003, 30(02): 250-250.
• Abstract ( 2075 ) HTML ( 934 ) PDF (36KB) ( 934 ) 　　　
• ‘Longjiao 2’is early maturity hot pepper Hybrid．Resistance to TMV and tolerance Phytophthora blight，The average yield is 52．3 t·hm^-2． It is suitable to plant under plastic film and in ope n field in north area．
  
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• Two New Hybrid Varieties of Michelia
• Gong Xun;;Zhang Guoli;Pan Yuezhi;Yang Zhiyun
• Acta Horticulturae Sinica. 2003, 30(02): 251-251.
• Abstract ( 1356 ) HTML ( 991 ) PDF (55KB) ( 991 ) 　　　
• Two new hybrid varieties of Michelia， ‘Chuju hanxiao’and ‘Chunyue hanxiao’，are selected from hybrid pupulation between Michelia ymuumensis and Michelia calcicola. The two new varieties are characterized by evergreen brush，large and fragrant flowers， long flowering date form Feb. to Apt.， strong adaptability to drought，poor soil and pest，and easy propagate．Both two varieties are good ornamental plants in gardens for enjoyilag flowers and leaves．
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