Study on Influencing Factor of Bulblet Enlargement in vitro of Asiatic Lily

doi:10.16420/j.issn.0513-353x.2024-0368

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (6): 1644-1660.doi: 10.16420/j.issn.0513-353x.2024-0368

• Cultivation?Physiology & Biochemistry • Previous Articles Next Articles

Study on Influencing Factor of Bulblet Enlargement in vitro of Asiatic Lily

WU Xiaodan¹, KONG Xiangfeng¹, GAO Li², GONG Tiangeng¹, PIAO Meiling¹, YU Pengcheng¹, and JIA Guixia¹^,^*()

¹ Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding，National Engineering Research Center for Floriculture，Beijing Laboratory of Urban and Rural Ecological Environment，Beijing Laboratory of Urban and Rural Ecological Environment，Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education，School of Landscape Architecture，Beijing Forestry University，Beijing 100083，China
² Beijing Green Garden Group Co，Ltd. Beijing 100067，China

Received:2024-09-01 Revised:2025-02-22 Online:2025-06-20 Published:2025-06-20
Contact: and JIA Guixia

Abstract

Abstract:

This study used tissue cultured bulblets of the new Asian lily cultivars‘Tianxin’and ‘Xiaohaixing’as materials to investigate the effects of 6-BA，NAA，and glyphosate on inducing adventitious buds on the scales. Based on the screening of suitable induction media for adventitious buds，the effects of plant growth regulators such as salicylic acid（SA），paclobutrazol（PP₃₃₃），and 2, 4-dichlorobrassinolide（2,4-EBR），sucrose，activated carbon（AC），NAA，6-BA，and basic media on the swelling of small bulbs were studied，and the expression changes of starch synthase genes SSS，SSS2，sugar efflux transporters genes SWEET14 and SWEET15 during the swelling development of small bulbs were analyzed. The results showed that‘Tianxin’and‘Xiaohaixing’germinated the most on MS + 0.5 mg · L^-1 6-BA + 0.2 mg · L^-1 NAA + 30 g · L^-1 sucrose medium，with differentiation coefficients of 2.78 and 2.03，respectively. The enlargement coefficients of‘Tianxin’in MS + 1.0 mg · L^-1 PP₃₃₃+ 0.05 mg · L^-1 2,4-EBR + 60 g · L^-1 sucrose，and MS + 0.5 mg · L^-1 6-BA + 0.1 mg · L^-1NAA + 60 g · L^-1 sucrose + 1.0 g · L^-1 activated carbon medium were 4.42，5.15，and 4.16，respectively. The suitable swelling medium for them was selected as MS + 1.0 mg · L^-1 NAA + 0.1-0.5 mg · L^-1 6-BA + 60-90 g · L^-1 sucrose + 1.0-2.0 g · L^-1 AC. The enlargement coefficients of‘Tianxin’and‘Xiaohaixing’in B5 medium were significantly higher than those in MS and WPM medium treatments，at 4.57 and 4.83，respectively. In B5 medium，the expression levels of soluble starch synthase genes SSS and SSS2，as well as sugar efflux transporters SWEET14 and SWEET15，gradually increase with the growth of the‘Tianxin’bulblet，reaching their highest levels during the rapid expansion phase of bulblet. In the leaves，the expression levels are highest at 36 days before entering the rapid expansion phase of bulblet.

Key words: Lilium, bulblet enlargement, tissue culture, carbohydrate metabolism

WU Xiaodan, KONG Xiangfeng, GAO Li, GONG Tiangeng, PIAO Meiling, YU Pengcheng, and JIA Guixia. Study on Influencing Factor of Bulblet Enlargement in vitro of Asiatic Lily[J]. Acta Horticulturae Sinica, 2025, 52(6): 1644-1660.

Figures/Tables 16

References 50

[1]	Chen L Q, Qu X Q, Hou B H, Sosso d, Osorio S, Fernie A R, Frommer W B. 2012. Sucrose efflux mediated by SWEET proteins as a key step for phloem transport. Science, 335 (6065):207-211.
[2]	Chen Shunan. 2018. Study on tissue culture and development of tube bubls in Lilium duchartrei[M. D. Dissertation]. Ya’an: Sichuan Agricultural University. (in Chinese)
	陈姝男. 2018. 宝兴百合组织培养和试管鳞茎膨大研究[硕士论文]. 雅安: 四川农业大学.
[3]	Chong J, Piron M C, Meyer S, Merdinoglu D, Bertsch C, Mestre P. 2014. The SWEET family of sugar transporters in grapevine:VvSWEET 4 is involved in the interaction with Botrytis cinerea. Journal of Experimental Botany, 65 (22):6589﹣6601.
[4]	Chou Shuo, Tang Fengluan, Xia Ke, Li Xiujuan, Zhao Zhiguo, Jiang Qinghong, Zhao Jian. 2017. Propagation culture and bulbs development of Lilium brownii var. viridulum. Guangdong Agricultural Sciences, 44 (4):61-66. (in Chinese)
	仇硕, 唐凤鸾, 夏科, 李秀娟, 赵志国, 蒋庆鸿, 赵健. 2017. 龙牙百合增殖培养及鳞茎生长发育研究. 广东农业科学, 44 (4): 61-66.
[5]	Dai Yue, Liu Llinya, Yang Na, Xiang Wenli, Zhou Yuan, Huang Yachen. 2021. Research advances on plant SWEET sugar transporters. Molecular Plant Breeding, 19 (4):1128-1135. (in Chinese)
	代玥, 刘林娅, 杨那, 向文丽, 周园, 黄亚成. 2021. 植物中SWEET蛋白的研究进展. 分子植物育种, 19 (4):1128-1135.
[6]	Feng C Y, Han J X, Han X X, Jiang J. 2015. Genome-wide identification,phylogeny,and expression analysis of the SWEET gene family in tomato. Gene, 573 (2):261-272.
[7]	Jeena G S, Kumar S, Shukla R K. 2019. Structure,evolution and diverse physiological roles of SWEET sugar transporters in plants. Plant Molecular Biology,100:351-365.
[8]	Huang Ziyang, Liu Jie, Kang Jie, Ren Ziming, Cui Qi, Li Dongze, Xia Yiping, Ma Si, Wu Yun. 2024. Advances in studies on sugar transporters in ornamental plants. Journal of Zhejiang University(Agriculture and Life Sciences), 50 (1):12-24. (in Chinese)
	黄子洋, 刘洁, 康婕, 任梓铭, 崔祺, 李东泽, 夏宜平, 马斯, 吴昀. 2024. 观赏植物糖转运蛋白研究进展. 浙江大学学报(农业与生命科学版), 50 (1):12-24.
[9]	Hui Zhenlong, Wang Di, Li Zongguo, Li Chaozhou, Li Xupeng, Zhang Junlian. 2014. Influences of exogenous salicylic acid on growth and resistance physiology of continuous cropping potato. Agricultural Research in the Arid Areas, 32 (4):1-8. (in Chinese)
	回振龙, 王蒂, 李宗国, 李朝周, 李旭鹏, 张俊莲. 2014. 外源水杨酸对连作马铃薯生长发育及抗性生理的影响. 干旱地区农业研究, 32 (4):1-8.
[10]	Li J, Chen D, Jiang G L, Song H Y, Tu M Y, Sun S X. 2020b. Molecular cloning and expression analysis of EjSWEET15,enconding for a sugar transporter from loquat. Scientia Horticulturae,272:109552.
[11]	Li X Y, Guo W, Wang A D. 2020a. Histone acetylation at the promoter for the transcription factor PuWRKY 31 affects sucrose accumulation in pear fruit. Plant Physiology, 182 (4):2035-2046.
[12]	Li Yujing, Feng Yuqing, Zhao Yuanyuan, Shi Hongzhi. 2023. Review of absorption and utilization of different nitrogen forms and their effects on plant physiological metabolism. Journal of Agricultural Science and Technology, 25 (2):128-139. (in Chinese)
	李玉静, 冯雨晴, 赵园园, 史宏志. 2023. 不同形态氮素吸收利用及其对植物生理代谢影响的综述. 中国农业科技导报, 25 (2):128-139.
[13]	Lian M L, Chakrabarty D, Paek K Y. 2003. Growth of Lilium oriental hybrid‘Casablanca’bulblet using bioreactor culture. Scientia Horticulturae, 97 (1):41-48.
[14]	Liu Yanju, Zhu Yongguan, Ding Hui, Tong Yiping. 2004. Relationship between SPAD values and contents NO₃-N in Spinacia oleracea. Journal of Agro-Environment Science,(3):484-487. (in Chinese)
	刘艳菊, 朱永官, 丁辉, 童依平. 2004. 不同氮肥水平下SPAD读数与菠菜硝态氮含量关系的初步研究. 农业环境科学学报,(3):484-487.
[15]	Lü Cuizhu, Wang Youguo, Wang Li. 2020. Study on the influencing factors of bud proliferation and bulblet swelling of Lilium fargesii in vitro. Journal of Southwest Forestry University(Natural Sciences), 40 (1):38-45. (in Chinese)
	吕翠竹, 王有国, 王立. 2020. 绿花百合组培芽增殖与试管鳞茎膨大的影响因素研究. 西南林业大学学报(自然科学), 40 (1):38-45.
[16]	Mabvongwe O, Manenji B T, Gwazane M, Chabdiposha M. 2016. The effect of paclobutrazol application time and variety on growth, yield, and quality of potato(Solanum tuberosum L.). Advances in Agriculture, 2016 (1):1585463.
[17]	Meier M, Liu Y, Lay-Pruitt K S, Takahashi H, Von W N. 2020. Auxin-mediated root branching is determined by the form of available nitrogen. Nature Plants, 6 (9):1136-1145.
[18]	Min Ruihan, Sun Minyi, Wu Yun, Li Shiqi, Xia Yiping. 2020. In vitro bulblet development and analysis of starch synthase gene(LohGBSSI)from Lilium‘Sorbonne’. Journal of Zhejiang A & F University, 37 (2):201-208. (in Chinese)
	闵芮涵, 吴昀, 李世琦, 夏宜平.孙敏译, 2020. 离体百合鳞茎发育及淀粉合成酶基因LohGBSSI的克隆与表达. 浙江农林大学学报, 37 (2):201-208.
[19]	Niu Huiting, Yang Daolan, Feng Weihong, Yin Yan, Li Aibing, Wang Lu, He Xiao. 2022. Effects of different ffactors on bulblet induction and enlargement in lanzhou lily bulb tissue cultivation. Gansu Agricultural Science and Technology, 53 (6):66-71. (in Chinese)
	牛慧婷, 杨道兰, 冯炜弘, 尹燕, 李爱兵, 王璐, 何潇. 2022. 不同因子对兰州百合组培小鳞茎诱导及膨大的影响研究. 甘肃农业科技, 53 (6):66-71.
[20]	Qi Xianyu, Zhang Wenliang, Yang Panpan, Xu Leifeng, Ming Jun. 2022. Optimization of high efficient tissue culture regeneration system for ornamental and edible dual purpose lily-‘Jinghe’. China Vegetables,(8):57-62. (in Chinese)
	祁先宇, 张文亮, 杨盼盼, 徐雷锋, 明军. 2022. 赏食兼用百合京鹤高效组培再生体系的优化. 中国蔬菜,(8):57-62.
[21]	Qin Xinhui, Cui Xinglin, Chen Xuehong, Liu Fen, Yi Wanwei. 2015. Study on the effect of different factors on the expansion of bulblets of Lilium davidii var.uniclor culture. Forest Science and Technology,(12):44-47. (in Chinese)
	秦新惠, 崔兴林, 陈学红, 刘芬, 伊万伟. 2015. 不同因子对兰州百合组培小鳞茎膨大的影响研究. 林业科技通讯,(12): 44-47.
[22]	Shen Ningdong, Wei Meiqin. 2008. Effect of SA on formation and growth of micro-bulb of Lilium brownie. Journal of Qinghai Normal University(Natural Science),(2):76-77. (in Chinese)
	沈宁东, 韦梅琴. 2008. 水杨酸在观赏百合试管培养中对鳞茎形成的影响. 青海师范大学学报(自然科学版),(2):76-77.
[23]	Shin K S, Chakrabarty D, Paek K Y. 2002. Sprouting rate,change of carbohydrate contents and related enzymes during cold treatment of lily bulblets regenerated in vitro. Scientia Horticulturae,96:195-204.
[24]	Su Di, Fang Zheng, Li Yingli, Zhao Bing. 2012. Effect of different NH₄⁺N/NO₃^--N ratios on the growth of Lilium. Northern Horticulture,(2):67-69. (in Chinese)
	苏頔, 方正, 李英丽, 赵斌. 2012. 不同氮素形态配比对百合生长发育的影响. 北方园艺,(2):67-69.
[25]	Tang Xinhua, Qu Zicheng, Zhang Hao, Wei Qiaorong, Zhang Lili, Shi Ying. 2018. Effect of physiology and yield by spraying EBR during potato tuber formation stage. Journal of Nuclear Agricultural Sciences, 32 (9):1855-1863. (in Chinese)
	唐鑫华, 曲自成, 张浩, 魏峭嵘, 张丽莉, 石瑛. 2018. 块茎形成期外施表油菜素内酯对马铃薯生理和产量的影响. 核农学报, 32 (9):1855-1863.
[26]	Thakur R, Sood A, Nagar P K, Pandey S, Sobti R, Ahuja P S. 2006. Regulation of growth of Lilium plantlets in liquid medium by application of paclobutrazol or ancymidol,for its amenability in a bioreactor system:growth parameters. Plant Cell Rep,25:382-391.
[27]	Wang Lidong, Liang Haiyan, Wang Yanru, Quan Xiaoqiang, Li Xiaoyu, Yan Xiaoli. 2023. Effects of different nitrogen forms and ratios on photosynthetic characteristics and growth in Cunninghamia lanceolata and Schima superba Seedlings. Journal of Sichuan Agricultural University, 41 (2):217-224. (in Chinese)
	王立冬, 梁海燕, 王燕茹, 全小强, 李小玉, 闫小莉. 2023. 不同氮素形态与配比对杉木和木荷幼苗光合特性及生长的影响. 四川农业大学学报, 41 (2):217-224.
[28]	Wang Xiaoli, Han Liqun, Liu Jie, Dong Fengli. 2011. Effects of AC and PP₃₃₃ on the development of the tube bulbs of Lilium cernuum. Journal of Anhui Agricultural Sciences, 39 (19):11429-11430. (in Chinese)
	王晓丽, 韩立群, 刘杰, 董凤丽. 2011. 活性炭和多效唑对垂花百合试管鳞茎膨大的影响. 安徽农业科学, 39 (19):11429-11430.
[29]	Wu Yinliang, Wang Hongxia, Yang Jun, Fan Weijuan, Yang Nan, Yin Minhao, Zhang Peng. 2017. Advances in storage root development and regulation in sweet potato[Ipomoea batatas(L.)Lam.] . Plant Physiology Journal, 53 (5):749-757. (in Chinese)
	吴银亮, 王红霞, 杨俊, 范维娟, 杨楠, 殷旻昊, 张鹏. 2017. 甘薯储藏根形成及其调控机制研究进展. 植物生理学报, 53 (5):749-757.
[30]	Wu Yun. 2016. The mechanisms of lily bulblet initiation and development based on the in vitro model system[Ph. D. Dissertation]: Hangzhou: Zhejiang University. (in Chinese)
	吴昀. 2016. 基于离体模式体系的百合小鳞茎发生发育机制研究[博士论文]: 杭州: 浙江大学.
[31]	Xu J, Li Q, Li Y, Yang L, Zhang Y, Cai Y. 2021. Effect of exogenous gibberellin,paclobutrazol,abscisic acid,and ethrel application on bulblet development in Lycoris radiata. Front Plant Sci,11:615547.
[32]	Xu J X, Li Q Z, Yang L Y, Li X, Wang Z, Zhang Y C. 2020a. Changes in carbohydrate metabolism and endogenous hormone regulation during bulblet initiation and development in Lycoris radiata. BMC Plant Biology,20:180.
[33]	Xu J X, Li Q Z, Yang L Y, Li X, Wang Z, Zhang Y C. 2020b. Changes in starch synthesis and metabolism within developing bulbs of Lycoris radiata during the vegetative growth stage. Journal of Plant Growth Regulation,39:785-794.
[34]	Xu Junxu, Li Qingzhu, Li Ye, Yang Liuyan, Li Xin, Wang Zhen, Zhang Yongchun. 2020. Differential expression of genes related to endogenous hormone during bulb development in Lycoris radiata. Acta Horticulturae Sinica, 47 (6):1126-1140. (in Chinese)
	许俊旭, 李青竹, 李叶, 杨柳燕, 李心, 王桢, 张永春. 2020. 石蒜鳞茎膨大过程中内源激素相关基因的差异表达研究. 园艺学报, 47 (6):1126-1140.
[35]	Xu Junxu, Li Qingzhu, Li Ye, Yang Liuyan, Zhang Yongchun, Cai Youming. 2021. Differential expression of genes related to carbohydrate metabolism during bulb development in Lycoris radiata. Journal of Nuclear Agricultural Sciences, 35 (10):2250-2257. (in Chinese)
	许俊旭, 李青竹, 李叶, 杨柳燕, 张永春, 蔡友铭. 2021. 石蒜鳞茎膨大过程中碳水化合物代谢相关基因的差异表达研究. 核农学报, 35 (10):2250-2257.
[36]	Yin Yan, Yang Daolan, Liang Yuwen, Feng Weihong, Niu Huiting, Li Aibing. 2023. Effects of nitrogen forms on growth and enlargement of Lilium davidii var. unicolor Bulblets in vitro. Scientific & Technical Information of Gansu, 52 (3):7-10. (in Chinese)
	尹燕, 杨道兰, 梁玉文, 冯炜弘, 牛慧婷, 李爱兵. 2023. 氮素形态对兰州百合试管鳞茎生长及膨大的影响. 甘肃科技纵横, 52 (3):7-10.
[37]	Zang Yuwen, Jiang Fangling, Cheng Yaqi, Kong Xiangyu, Wu Zhen. 2016. Study on the dynamic changes of the major carbohydrate content and the related enzyme activities during the microcorm development of Colocasia esculenta. Acta Botanica Boreali-Occidentalia Sinica, 36 (4):700-705. (in Chinese)
	臧玉文, 蒋芳玲, 程雅琪, 孔祥宇, 吴震. 2016. 红香芋试管球茎膨大过程中主要碳水化合物含量以及淀粉合成相关酶活性的动态研究. 西北植物学报, 36 (4):700-705.
[38]	Zhang Jie, Cai Xuanmei, Lin Zhen, Guo Wenjie, Fang Shaozhong. 2010. Formation and enlargement of lily bulblet in test tube. Fujian Journal of Agricultural Sciences, 25 (3):328-331. (in Chinese)
	张洁, 蔡宣梅, 林真, 郭文杰, 方少忠. 2010. 百合试管鳞茎诱导及膨大技术的研究. 福建农业学报, 25 (3):328-331.
[39]	Zhang Jinzhong. 2018. Studies on in vitro development of Lilium bulblet and enzymes related to its starch synthesis[Ph. D. Dissertation]. Guangzhou: South China Agricultural University. (in Chinese)
	张进忠. 2018. 百合鳞茎离体发育及淀粉合成相关酶研究[博士论文]. 广州: 华南农业大学.
[40]	Zhang Jinzhong, Sun Jiaman, Li Chaosheng, Wei Liping, Fan Yanping. 2019. Cloning of starch synthesis-related enzyme gene and its expression analysis in process of bulblet development of Lilium. Guihaia, 39 (4):446-452. (in Chinese)
	张进忠, 孙嘉曼, 李朝生, 韦莉萍, 范燕萍. 2019. 百合鳞茎发育过程中淀粉合成相关酶基因的克隆及表达分析. 广西植物, 39 (4):446-452.
[41]	Zhang Jiyu, Wang Gang, Wang Tao, Jia Zhanhui, Xuan Jiping. 2023. Research progress on functional roles of SWEET proteins in plant growth and development. Journal of Plant Resources and Environment, 32 (5):1-15. (in Chinese)
	张计育, 王刚, 王涛, 贾展慧, 宣继萍. 2023. SWEET蛋白在植物生长发育中的功能作用研究进展. 植物资源与环境学报, 32 (5): 1-15.
[42]	Zhang Lin. 2018. Histological and transcriptome analysis of de novo bulblet regeneration in Lilium spp[Ph. D. Dissertation]. Hangzhou: Zhejiang University. (in Chinese)
	张琳. 2018. 百合小鳞茎从头再生的组织学和转录组学研究[博士论文]. 杭州: 浙江大学.
[43]	Zhang Minhui, Chen Junji, Quan Wei, Gu Zhijia, Wu Hongzhi. 2024. Study on influencing factors of bulblet enlargement in vitro of Lilium amoenum. Journal of Zhejiang University(Agriculture and Life Sciences), 50 (1):42-52. (in Chinese)
	张敏慧, 陈俊吉, 全伟, 谷志佳, 吴红芝. 2024. 玫红百合组培小鳞茎瓶内膨大影响因子的研究. 浙江大学学报(农业与生命科学版), 50 (1):42-52.
[44]	Zhang X S, Feng C Y, Wang M, Li T L, Liu X. 2021. Plasma membrane-localized SlSWEET7a and SlSWEET14 regulate sugar transport and storage in tomato fruits. Horticulture Research, 8 (1):186.
[45]	Zhang Yanni, Li Zhaoting, Zhang Yanbo, Wang Haiyang. 2016. Culture optimization of test tube bulb formation and expansion of Lilium dauricum. Jiangsu Agricultural Sciences, 44 (4):74-78. (in Chinese)
	张彦妮, 李兆婷, 张艳波, 汪海洋. 2016. 毛百合试管鳞茎形成和膨大的培养优化. 江苏农业科学, 44 (4):74-78.
[46]	Zhao Jiamin, Qi Hongying, Li Yun, Zhang Jiamei, Yin Bangguo. 2017. Bulblet induction in vitro of Lilium asiatic hybrids. Heilongjiang Agricultural Sciences,(2):22-25. (in Chinese)
	赵家敏, 祁宏英, 李赟, 张佳美, 尹帮果. 2017. 亚洲百合试管鳞茎诱导. 黑龙江农业科学,(2):22-25.
[47]	Zhen Q L, Fang T, Peng Q, Liao L, Zhao L, Owtti A. Han Y P. 2018. Developing gene-tagged molecular markers for evaluation of genetic association of apple SWEET genes with fruit sugar accumulation. Horticulture Research, 5 (1):14.
[48]	Zheng Qianming, Wang Xiaoke, Wang Honglin, Jie Pu, Fu Huimin, Chen Chuanwu. 2023. Expression pattern and transportation activity test of sugar transporter gene CitSWEET15 from sweet orange. Chinese Journal of Tropical Crops, 44 (10):1944-1951. (in Chinese)
	郑乾明, 王小柯, 王红林, 解璞, 付慧敏, 陈传武. 2023. 甜橙糖转运蛋白CitSWEET15基因的表达分析与转运活性检测. 热带作物学报, 44 (10):1944-1951.
[49]	Zhou Lingyun, Gao Suping, Chen Feng. 2016. Action mechanism of sucrose and photoperiod on bulblet enlargement of Lilium sargentiae. Journal of Zhejiang University(Agriculture and Life Sciences), 42 (4):435-441. (in Chinese)
	周玲云, 高素萍, 陈锋. 2016. 蔗糖和光周期在泸定百合试管鳞茎膨大中的作用机制. 浙江大学学报(农业与生命科学版), 42 (4):435-441.
[50]	Zhou Peng, Peng Futian, Wei Shaochong, Peng Yong. 2007. Effects of rhizosphere nitrate and ammonium on the level of cytokinins and leaf growth of Malus hupenensis rehd. Acta Horticulturae Sinica, 34 (2):269-274. (in Chinese)
	周鹏, 彭福田, 魏绍冲, 彭勇. 2007. 氮素形态对平邑甜茶细胞分裂素水平和叶片生长的影响. 园艺学报, 34 (2):269-274.

基因名称 Gene name	正向引物（5′-3′） Forward primer sequence	反向引物（5′-3′） Reverse primer sequence
SSS（CL7226_C1）	AATGGTTGGAGGTGGAAATCT	GGCCTCCTTCGGAACATAAA
SSS2（CL3977_C2）	CACTGGTGGCCTTAGAGATAC	CCAGCATACTCTCCTTCGATAAA
SWEET14（CL255_C3）	TGACTCTTCTTGGTTGGATCTG	GGCATGAACTCGACACTCTT
SWEET15（CL7570_C1）	GGGTTCCAATCTCTTCCCTATG	TCCGATCGAGTTAATGGTGATG
Actin（内参基因 Reference gene）	CCCATTGAGCACGGCATTGTC	GGATTGAGAGGAGCTTCGGTGAGA

基因名称 Gene name	正向引物（5′-3′） Forward primer sequence	反向引物（5′-3′） Reverse primer sequence
SSS（CL7226_C1）	AATGGTTGGAGGTGGAAATCT	GGCCTCCTTCGGAACATAAA
SSS2（CL3977_C2）	CACTGGTGGCCTTAGAGATAC	CCAGCATACTCTCCTTCGATAAA
SWEET14（CL255_C3）	TGACTCTTCTTGGTTGGATCTG	GGCATGAACTCGACACTCTT
SWEET15（CL7570_C1）	GGGTTCCAATCTCTTCCCTATG	TCCGATCGAGTTAATGGTGATG
Actin（内参基因 Reference gene）	CCCATTGAGCACGGCATTGTC	GGATTGAGAGGAGCTTCGGTGAGA

处理 Treatment	生长调节剂/（mg · L^-1） Growth regulator			甜心 Tianxin			小海星 Xiaohaixing
处理 Treatment	6-BA	NAA	6-BA/ NAA	出芽时间/d Budding time	诱导率 Inductivity	分化系数 Coefficient of differentiation	出芽时间/d Budding time	诱导率 Inductivity	分化系数 Coefficient of differentiation
1	0.5	0.1	5.0	8 ~ 24	1.00 ± 0 a	2.44 ± 0.25 abc	13 ~ 30	0.90 ± 0.11 ab	1.92 ± 0.29 a
2	0.5	0.2	2.2	8 ~ 24	0.90 ± 0.09 ab	2.78 ± 0.69 a	13 ~ 30	0.89 ± 0.01 ab	2.03 ± 0.18 a
3	0.5	1.0	0.5	14 ~ 30	0.82 ± 0.08 abc	2.14 ± 0.29 abcd	24 ~ 45	0.37 ± 0.10 de	1.25 ± 0.45 bcd
4	1.0	0.1	10.0	14 ~ 30	0.96 ± 0.06 a	2.52 ± 0.63 ab	18 ~ 35	0.84 ± 0.05 abc	2.01 ± 0.16 a
5	1.0	0.2	5.0	14 ~ 30	0.89 ± 0.12 ab	2.12 ± 0.51 abcd	18 ~ 35	0.83 ± 0.13 abc	1.72 ± 0.54 ab
6	1.0	1.0	1.0	20 ~ 35	0.74 ± 0.18 bcd	1.55 ± 0.16 d	30 ~ 55	0.25 ± 0.15 ef	1.22 ± 0.40 bcd
7	1.5	0.1	15.0	18 ~ 35	0.92 ± 0.07 ab	2.73 ± 0.25 a	24 ~ 45	0.92 ± 0.06 a	1.81 ± 0.45 ab
8	1.5	0.2	7.5	18 ~ 35	0.89 ± 0.09 ab	2.09 ± 0.19 abcd	24 ~ 45	0.75 ± 0.13 bc	1.94 ± 0.65 a
9	1.5	1.0	1.5	24 ~ 45	0.63 ± 0.07 d	1.60 ± 0.40 d	30 ~ 50	0.19 ± 0.17 fg	1.01 ± 0.58 cd
10	2.0	0.1	20.0	24 ~ 45	0.83 ± 0.16 abc	2.46 ± 0.64 abc	24 ~ 45	0.71 ± 0.14 c	1.64 ± 0.38 abc
11	2.0	0.2	10.0	24 ~ 45	0.74 ± 0.12 bcd	1.82 ± 0.17 cd	24 ~ 45	0.47 ± 0.04 d	1.55 ± 0.41 abc
12	2.0	1.0	2.0	24 ~ 45	0.66 ± 0.22 cd	1.89 ± 0.32 bcd	40 ~ 65	0.08 ± 0.06 g	0.67 ± 0.52 d

处理 Treatment	生长调节剂/（mg · L^-1） Growth regulator			甜心 Tianxin			小海星 Xiaohaixing
处理 Treatment	6-BA	NAA	6-BA/ NAA	出芽时间/d Budding time	诱导率 Inductivity	分化系数 Coefficient of differentiation	出芽时间/d Budding time	诱导率 Inductivity	分化系数 Coefficient of differentiation
1	0.5	0.1	5.0	8 ~ 24	1.00 ± 0 a	2.44 ± 0.25 abc	13 ~ 30	0.90 ± 0.11 ab	1.92 ± 0.29 a
2	0.5	0.2	2.2	8 ~ 24	0.90 ± 0.09 ab	2.78 ± 0.69 a	13 ~ 30	0.89 ± 0.01 ab	2.03 ± 0.18 a
3	0.5	1.0	0.5	14 ~ 30	0.82 ± 0.08 abc	2.14 ± 0.29 abcd	24 ~ 45	0.37 ± 0.10 de	1.25 ± 0.45 bcd
4	1.0	0.1	10.0	14 ~ 30	0.96 ± 0.06 a	2.52 ± 0.63 ab	18 ~ 35	0.84 ± 0.05 abc	2.01 ± 0.16 a
5	1.0	0.2	5.0	14 ~ 30	0.89 ± 0.12 ab	2.12 ± 0.51 abcd	18 ~ 35	0.83 ± 0.13 abc	1.72 ± 0.54 ab
6	1.0	1.0	1.0	20 ~ 35	0.74 ± 0.18 bcd	1.55 ± 0.16 d	30 ~ 55	0.25 ± 0.15 ef	1.22 ± 0.40 bcd
7	1.5	0.1	15.0	18 ~ 35	0.92 ± 0.07 ab	2.73 ± 0.25 a	24 ~ 45	0.92 ± 0.06 a	1.81 ± 0.45 ab
8	1.5	0.2	7.5	18 ~ 35	0.89 ± 0.09 ab	2.09 ± 0.19 abcd	24 ~ 45	0.75 ± 0.13 bc	1.94 ± 0.65 a
9	1.5	1.0	1.5	24 ~ 45	0.63 ± 0.07 d	1.60 ± 0.40 d	30 ~ 50	0.19 ± 0.17 fg	1.01 ± 0.58 cd
10	2.0	0.1	20.0	24 ~ 45	0.83 ± 0.16 abc	2.46 ± 0.64 abc	24 ~ 45	0.71 ± 0.14 c	1.64 ± 0.38 abc
11	2.0	0.2	10.0	24 ~ 45	0.74 ± 0.12 bcd	1.82 ± 0.17 cd	24 ~ 45	0.47 ± 0.04 d	1.55 ± 0.41 abc
12	2.0	1.0	2.0	24 ~ 45	0.66 ± 0.22 cd	1.89 ± 0.32 bcd	40 ~ 65	0.08 ± 0.06 g	0.67 ± 0.52 d

	因子Factor	平方和SS	自由度df	均方MS	F	P
甜心 Tianxin	6-BA	1.301	3	0.434	2.413	0.081
	NAA	4.832	2	2.416	13.443	0.000^**
	6-BA × NAA	1.774	6	0.296	1.645	0.161
	误差Error	7.009	39	0.180
	总计Total	254.532	51
小海星Xiaohaixing	6-BA	1.709	3	0.570	2.774	0.051
	NAA	8.709	2	4.354	21.204	0.000^**
	6-BA × NAA	0.456	6	0.076	0.370	0.894
	误差Error	9.857	48	0.205
	总计Total	158.494	60

Study on Influencing Factor of Bulblet Enlargement in vitro of Asiatic Lily

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 16

References 50

Related Articles 15

Recommended Articles

Metrics

Comments

草甘膦/mg · L^-1 Glyphosate	‘甜心’‘Tianxin’		‘小海星’‘Xiaohaixing’
草甘膦/mg · L^-1 Glyphosate	诱导率 Inductivity	分化系数 Coefficient of differentiation	诱导率 Inductivity	分化系数 Coefficient of differentiation
0（对照 Control）	0.89 ± 0 ab	2.26 ± 0.39 ab	0.89 ± 0.11 ab	2.59 ± 0.82
1.0	0.93 ± 0.13 a	2.70 ± 0.93 a	0.96 ± 0.06 a	3.56 ± 1.34
2.0	0.71 ± 0.06 bc	1.44 ± 0.12 b	0.78 ± 0.11 ab	2.51 ± 0.56
3.0	0.56 ± 0.12 c	1.30 ± 0.50 b	0.74 ± 0.06 b	2.33 ± 0.51

处理 Treatments	NAA/（mg · L^-1）	蔗糖/（g · L^-1） Sucrose	AC/（g · L^-1）	6-BA/（mg · L^-1）	膨大系数Expansion coefficient
处理 Treatments	NAA/（mg · L^-1）	蔗糖/（g · L^-1） Sucrose	AC/（g · L^-1）	6-BA/（mg · L^-1）	甜心Tianxin	小海星Xiaohaixing
1	0.1	30	0.5	0.1	2.67 ± 0.73 de	3.05 ± 1.26 de
2	0.1	60	1.0	0.5	4.16 ± 1.34 a	4.12 ± 1.47 abc
3	0.1	90	2.0	1.0	3.27 ± 1.54 bcd	3.85 ± 0.91 abcd
4	0.5	30	1.0	1.0	2.40 ± 0.60 e	2.95 ± 0.78 e
5	0.5	60	2.0	0.1	3.63 ± 1.24 abc	4.25 ± 1.28 abc
6	0.5	90	0.5	0.5	3.91 ± 1.64 ab	3.55 ± 0.69 bcde
7	1.0	30	2.0	0.5	2.96 ± 0.79 cde	4.35 ± 1.17 ab
8	1.0	60	0.5	1.0	2.79 ± 0.91 de	3.43 ± 0.88 cde
9	1.0	90	1.0	0.1	3.13 ± 1.11 bcde	4.43 ± 1.65 a

[1]	LIU Guicai , DENG Sha, DONG Yunchang , LUO Weicomg , GAO Huanhuan , JIANG Liangwei , FANG Lin , LI Lin , WU Kunlin , and ZENG Songjun, . A New Hippeastrum Cultivar‘Hua Jin’ [J]. Acta Horticulturae Sinica, 2023, 50(S1): 131-132.
[2]	GUO Manna, DENG Sha, FANG Lin , LI Lin , WU Kunlin , and ZENG Songjun, . A New Hippeastrum Cultivar‘Zhong Ke Xiang Fei’ [J]. Acta Horticulturae Sinica, 2023, 50(S1): 133-134.
[3]	ZHANG Wanqing, ZHANG Hongxiao, LIAN Xiaofang, LI Yuying, GUO Lili, HOU Xiaogai. Analysis of DNA Methylation Related to Callus Differentiation and Rooting Induction of Paeonia ostii‘Fengdan’ [J]. Acta Horticulturae Sinica, 2022, 49(8): 1735-1746.
[4]	WANG Jing, XU Leifeng, WANG Ling, QI Xianyu, SONG Meng, CAO Yuwei, HE Guoren, TANG Yuchao, YANG Panpan, MING Jun. The Numerical Classification of Flower Color Phenotype in Lily [J]. Acta Horticulturae Sinica, 2022, 49(3): 571-580.
[5]	ZHOU Huiming, MO Zhilong, CHEN Changming, LIN Huifeng. A New Cymbidium Cultivar‘Shayang Cuidie’ [J]. Acta Horticulturae Sinica, 2022, 49(12): 2767-2768.
[6]	ZHANG Wenxin, CAO Xueying, ZHOU Shiming, ZHANG Bieyu, FANG Lin, LI Lin, WU Kunlin, and ZENG Songjun, . A New Hippeastrum Cultivar‘Shengyin 3’ [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2909-2910.
[7]	CAO Xueying, ZHANG Wenxin, ZHOU Jin, ZHOU Shiming, ZHANG Bieyu, FANG Lin, LI Lin, WU Kunlin, and ZENG Songjun, . A New Hippeastrum Cultivar‘Shengyin 4’ [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2911-2912.
[8]	GUAN Minhua, CAO Xueying, ZHANG Wenxin, YE Yuzhuang, FANG Lin, LI Lin, WU Kunlin, and ZENG Songjun, . A New Hippeastrum Cultivar‘Shengyin 5’ [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2913-2914.
[9]	DENG Yi, ZHANG Wenxin, CAO Xueying, FANG Lin, LI Lin, WU Kunlin, LIU Haitao, , and ZENG Songjun, . A New Hippeastrum Cultivar‘Shuangyan’ [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2915-2916.
[10]	YU Pengcheng, TAN Pingyu, GAO Li, JIA Guixia. Analysis of the Development of Flower Color Based on Hybridization Breeding Process in Lilium OT Hybrids [J]. Acta Horticulturae Sinica, 2021, 48(10): 1885-1894.
[11]	BI Mengmeng, CAO Yuwei, SONG Meng, TANG Yuchao, HE Guoren, YANG Yue, YANG Panpan, XU Leifeng, MING Jun. Advances in Flower Color Research of Lilium [J]. Acta Horticulturae Sinica, 2021, 48(10): 2073-2086.
[12]	LIAO Xiaoshan, ZHOU Jun, RONG Duoyan, LIN Qindan, DENG Yawen, and ZHENG Sixiang, . A New Lilium Oriental Cultivar‘ChangErbenyue’ [J]. Acta Horticulturae Sinica, 2020, 47(S2): 3045-3046.
[13]	YANG Xiaofang, ZHANG Zuying, MIAO Lixiang, ZHANG Yuchao, SHEN Lan, QIN Qiaoping, and JIANG Guihua, . Preliminary Analysis on the Molecular Differences of Fruit Colouring Between Yuexin Strawberry and Its Somaclonal Mutation [J]. Acta Horticulturae Sinica, 2020, 47(10): 1999-2008.
[14]	DENG Sha1,2，CAO Xueying3,4，ZHENG Feng1，ZHANG Wenxin3,4，ZHOU Shiming3,4，ZHANG Bieyu3,4，ZHANG Jinlong3,4，FANG Lin1，LI Lin1，WU Kunlin1，and ZENG Songjun1,5,*. A New Hippeastrum Cultivar‘Shengyin 1’ [J]. ACTA HORTICULTURAE SINICA, 2019, 46(S2): 2865-2866.
[15]	DENG Sha1,2，ZHANG Wenxin3,4，ZHENG Feng1，CAO Xueying3,4，ZHOU Shiming3,4，ZHANG Bieyu3,4，ZHANG Jinlong3,4，FANG Lin1，LI Lin1，WU Kunlin1，and ZENG Songjun1,5,*. A New Hippeastrum Cultivar‘Shengyin 2’ [J]. ACTA HORTICULTURAE SINICA, 2019, 46(S2): 2867-2868.