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Current Issue

2004, Vol.31, No.4 Previous Issue    Next Issue

研究论文

  • Studies on the Utilization Potential of Two Somatic Hybrid Rootstocks andthe Biochemical Mechanism of Rootstock．scion Interaction
  • Zhou Kaibing;Guo Wenwu;Xia Renxue;Hu Liming;Huang Renhua
  • Acta Horticulturae Sinica. 2004, 31(4): 427-432.
  • Abstract ( 1553 ) HTML ( 1198 ) PDF (259KB) ( 1198 ) 　　　
  • It was conducted with ‘Guoqing 4’Satsuma mandarin two-year．old young tree whose rootstocks were Trifoliate(CK)，Troyer Citrange，Swingle Citrumelo，and Red tangerine+Trifoliate，Red tange-rine +Rough lemon somatic hybrids．Studies on the effects of rootstocks on the growth and the activities of antioxidant enzymes through potted experiment．The results are the following．The four kinds of treatments grew more vigorously than CK．The number of blossoms of Red tangerine+Trifoliate was higher than CK．and the other three were to the contrary．Rootstocks did not influence the annual changes in the content of soluble pro．tein and the activity of CAT．They bviously influence the annual changes in the activities of SOD and POD．In different periods，rootstocks notably influenced the biochemical indexes(except for SOD in leaf in April)．During the corresponding periods，the POD activities were negatively correlated with the shoot growth and the canopy volume．In December，there existed significant difference in the biochemical indexes among various rootstocks．The POD and the SOD activities in roots were respectively positively corelated with that in leaves．The utilization potential of these two somatic hybrids were discussed．
  • Related Articles | Metrics
  • The Efect of Inhibited Ph0t0respirati0n on Photoinh ibition in ‘CabernetSauvignon’(Vinefera L．CV．)under Drought Stress
  • Guan Xueqiang;Zhao Shijie;Li Dequan;and Shu Huairui
  • Acta Horticulturae Sinica. 2004, 31(4): 433-436.
  • Abstract ( 1703 ) HTML ( 1367 ) PDF (172KB) ( 1367 ) 　　　
  • Using two—year·old potted ‘Cabernet Sauvignon’(Vitls vinifera L．CV．)as materials，maxi—mal(Fv／Fm)and actual( PSII)photochemical eficiency of photosystem II(PSII)，net assimilation rate (Pn)were measured by combined measurements of gas exchange and chlorophyll fluorescence parameters after
    a 20 days slight，moderate and severe drought level of soil and succeeding three days INH·spraying treatment．Total electron transport rate(JT)，electron transport flows used in carboxylation(Jc)and oxygenation(J0) reaction catMyzed by Rubisco，and photorespiration rate(Pr)were calculated．Serious photoinhibition can be avoided by effective photoprotection mechanism in ‘Cabernet Sauvignon’，while photoinhibition aggravated when the leaves were sprayed with INH，a inhibitor of photorespiration，which suggests that photorespiraion plays an important role in photoprotection mechanism of ‘Cabernet Sauvignon’under drought stress．
  • Related Articles | Metrics
  • Efect of Exogenous Polyamines on Female and M ale Flower Diferentiationand Content of Endogenous Polyamines in Leaves of W alnut
  • Xu Jizhong;Chen Haijiang;Li Xiaodong;Zhang Zhihua;and Wang Yanhui
  • Acta Horticulturae Sinica. 2004, 31(4): 437-440.
  • Abstract ( 1514 ) HTML ( 1627 ) PDF (177KB) ( 1627 ) 　　　
  • A walnut cuhivar ‘Liaoning 1’ was used to study the effect of exogenous polyamines on fe．male and male flower differentiation and the content of endogenous polyamines in leaves．The results in two years showed that application of Put or Spd at 1×10-3mol·L-1。and 1×10-4 mol·L-1 for two times could markedly increase the numbers of female flower and raise the ratio of floral sexuality．The content of endoge．nous Put，Spd and Spm in the mixed buds increased and had a peak at the physiological stage of female flower
    buds．The content of endogenous Put in the leaves reached a peak but that of Spd and Spm changed slightly during the stage． Spraying with 1×10-3 mol·L-1 Put or Spd could increase the content of endogenous poly—amines in the leaves．Endogenous Put and Spd levels were affected at earlier time．while Spm at later time．
  • Related Articles | Metrics
  • Conservation of in Vitro Shoots of Persimmon and Date Plum by SlowGrowth and Genetic Stability of Recovered Plantlets
  • Ai Pengfei and Luo Zhengrong
  • Acta Horticulturae Sinica. 2004, 31(4): 441-450.
  • Abstract ( 1712 ) HTML ( 1775 ) PDF (618KB) ( 1775 ) 　　　
  • Studies on conservation of in vitro shoots of persimmon (Diospyros kaki Thunb．)and date plum (D．1otus L．)by slow growth and genetic stability of recovered plantlets were carried out．Results showed that 90％ of in vitro shoots conserved on MS(1／2N)supplemented with 20 L sucrose，7 g／L agar
    and 500 mg／L soluble PVP added with 20 L mannitol or 1．0 nqIg／L PP333 respectively or on 1／2MS(1／2N) supplemented with 15 g／L sucrose．7 g／L agar and 500 mg／L soluble PVP added with 0．2 mg／L CPPU f0r l8 months at(6±1)℃and 800 lX(12 h photoperiod)maintained viability．Those recovered plantlets conserved with 3 methods above were analyzed on cytological and molecular levels respectively．Resuh showed both Ha—clear DNA content and chromosome numbers maintained as norma1．And only few shoots cultivated with PP11，in which existed 0．1 6％ variation in Jirou persimmon owing to 3 distinct new bands appearing in l 827 ampli—fled bands and 0．86％ variation in Date plum for l distinct new band and absence of 14 bands existing in 1 736
    amplified bands when 3 single—bud sibling lines were analyzed by RAPD markers respectively．Thus．it sugges—ted these procedures would be effective and viable to conserve Diospyros germ plasm by slow growth as above．
  • Related Articles | Metrics
  • KCIO3 Induces Floral Bud Formation in Longan and the Changes of Endoge—nous Hormones
  • Chen Qingxi and Li Songgang
  • Acta Horticulturae Sinica. 2004, 31(4): 451-455.
  • Abstract ( 1518 ) HTML ( 1443 ) PDF (200KB) ( 1443 ) 　　　
  • Longan(Diraocarpus longan Lour．)trees were applied with potassium chlorate(KCIO3)in soil from April to August．The changes of endogenous hormones in leaves were determ ined during floral bud form ation．The results were as follows：longan applied with KC101 was induced floral bud formation and blos．somed after about 60 days． The inductive effect was more distinctive when CTK was appended in KC101． The contents of ABA and ZRs in leaves were in high level during physiological differentiation in treatment B(KC103+CTK)and D (KC10 )，and then fell down；however the level was low in contro1．It was pro．posed in this paper that the ratio of ZRs／GA1+3 was one could be the critical index of longan floral bud physio．1ogical differentiation．
  • Related Articles | Metrics

研究简报

研究论文

  • Studies on Changes tilizer Experiment of Field-Vegetable System under Longterm Fixed Fer-(III)Changes of Vegetable Yield and Nutrient Absorption under Diferent Fertilization Systems
  • Ge Xiaoguang;Gao Hui;Zhang Enping;Wang Xiaoxue;and Zhang Xin
  • Acta Horticulturae Sinica. 2004, 31(4): 456-460.
  • Abstract ( 1582 ) HTML ( 1376 ) PDF (227KB) ( 1376 ) 　　　
  • Results of long．term fertilization trial showed that application of organic manure alone increased soil fertility．the increase of biomass was not much，but economic yield was much higher than that iqcontro1．The improvement of yield was limited without nitrogen fertilizer application．Over several years’
    cultivation with organic manure and nitrogen fertilizer potassium amount in soil turned out insufficient，therefore there was an apparent increase of yield with combined application of potassium and nitrogen．There was almost no effect for yield improvement with application of phosphorus．Extra phosphorus application resulted in less yields under suficient phosphorus condition．On the base of observation and analysis of our long-term fertilization trial，“the regular of one third capacity of nutrient-yield” and effective standard for nitrogen，phosphorus and potassium application were put forward． Nutrients were used economically when soil fertility or yield WaS quite high．There was a range for parameters of fertilizer requirement，SO it should be adjusted according to soil fertility and yield．
  • Related Articles | Metrics
  • The Efect of Selenium---Sulphur Cooperated Spraying on the Nutrition Qualityof Garlic(Allium sativum L．)
  • Wang Changquan;Li Bing;and Li Huanxiu
  • Acta Horticulturae Sinica. 2004, 31(4): 461-466.
  • Abstract ( 1675 ) HTML ( 1404 ) PDF (250KB) ( 1404 ) 　　　
  • The nutritional function of different Se levels and the Se—S interaction were studied on the condition of spraying fixed S and different level Se on the garlic(Allium sativum L．)．1 1 mineral elements，4 kinds of quality components were determined． The trial data were analyzed by the Principal Component Analysis(PCA)and the Factor Analysis(FA)．The result showed，the main mineral factors(X1、X，、X )model accounted for 83．572％ of the total variation in the data．The linearity relation between the nutri．tional quality and main mineral factors were simulated．and the relation showed ll mineral elements c0uld be divided into 3 common factors(allicin factor，Se factor and Vitamin C factor)which significantly related to the garlic quality．Se，S and Se—S cooperated spraying improved the garlic nutrition quality(organic Se，
    ratio of organic Se，vitamin C and allicin)by increasing mineral nutrition．And if the available Se and S Were sufficient in the soil，the positive effects of S nutrition under the lOW and medium level Se(Na，SeO 20—40 mg·L-1)cooperated with the fixed level S[(NH4)2SO4 2500 mg ·L-1were found，namely the Se—Sproportion were about 1：125—62．5．
  • Related Articles | Metrics

研究简报

研究论文

  • Production of the Ogura Cytoplasmic Male Sterile(CMS)Lines of ChineseCabbage-pak-choi(Brassica campestris L．ssp．chinensis var．communis)and Turnip(B．campestris L．ssp．rapifera)and Cytological Observation ofTheir Sterile Organs
  • Cui Huimei;Cao Jiashu;Zhang Minglong;Yao Xiangtan ．and Xiang Xun
  • Acta Horticulturae Sinica. 2004, 31(4): 467-471.
  • Abstract ( 2043 ) HTML ( 1735 ) PDF (760KB) ( 1735 ) 　　　
  • The Ogura CMS lines of Chinese cabbage—pak．choi f Br~sica campestris L．ssp．chinensis
    Makino var．cornmunis Tsen et Lee．)and turnip[B．campestris L．ssp．rap r(z(Metzg．)Sinsk]were ob．
    tained through interspeeifie hybridization between Ogura CMS in B． nap us and Chinese cabbage．pak．choi and
    turnip，and successive backcrosses with Chinese cabbage—pak—choi and turnip as male parents． namely ‘Xin．
    xuan Ogu28-4’， ‘Aijiaohuang Ogu33—14’， ‘Naibing Ogu18-4’．Cytomorphological studies indicated that
    the inhibition of Ogura CMS B．campestris anther development may occur at the tetrad．uninucleic pollen stage．
    The microspores stop developing at the uninucleic stage， quickly degenerated to disappear， and abort
    absolutely before blossom． Tapetum of the male sterile line began vascuolation during meiosis and did n0t
    disappear even when the uninucleic microspores had degenerated while that of the male fenile line began to
    generate at tetrad stage and disappear after the pollens had matured． The different nuclear background 0f
    Ogura CMS lines had similar abortive character．It might indicate that the relation of nueleo．cvt0plasmic inter
    action iS steady，and the cytoplasmic effects were hardly affected by the nucleus of the sterile】ines．
  • Related Articles | Metrics
  • Efect of Diferent Nitrogen Applied on the Distribution of Carbon and Nitrogenin Sweet Pepper
  • Sui Fanggong;LU Yinyan’;W ang Yunhua;Zhou Guangsheng;and Chishaki Naoya
  • Acta Horticulturae Sinica. 2004, 31(4): 472-476.
  • Abstract ( 1496 ) HTML ( 965 ) PDF (362KB) ( 965 ) 　　　
  • Sweet pepper(Capsicum annuum L．)was used to study the effect of different nitrogen applied on the distribution of carbon and nitrogen assimilated and absorbed during fruit harvest period by using N and C double tracer echnique．The results obtained are as follows：The distributed ratio of the N absorbed at early fruiting stage Oil the fruit was 50．49％ at lower N and it was higher significantly than that at the
    moderate and the higher N treatments．Nitrogen nutrition not only controlled the distribution of N absorbed on
    the different organs．but also affected its translocation．Nitrogen nutrition affected hardly the distribution of”C
    assimilated among the organs but controlled the translocation and redistribution of”C，and as a result，the
    moderate N was most beneficial to the redistribution of”C up to fruit．The balance of carbon and nitrogen iS
    favorable to the accumulation and translocation to the reproductive organ．It iS why that the yield iS higher ear—
    lier stage than after stage at lower N，but adversely at higher N and only at moderate N either of all is higher．
  • Related Articles | Metrics

研究简报

研究论文

  • Efects of W ater Supply Status in M edia on C， N M etabolism of TomatoPotting Seedlings and the Relationship with Growth and Development
  • Cui Xiumin and Wang Xiufeng
  • Acta Horticulturae Sinica. 2004, 31(4): 477-481.
  • Abstract ( 1747 ) HTML ( 1269 ) PDF (225KB) ( 1269 ) 　　　
  • Tomato potting seedlings were treated under different floor level of water supply in media
    (RWC was 90％ ，75％ ，60％ ，45％ ，30％ respectively)，and discussed the relationship between tt1e
    changes of C，N metabolic products and development． The main results were as below：soluble sugar and
    reduetive sugar usually as carbon metabolic materials and osmotic solutes． increased in lear with water supply
    decreasing，whieh enhanced adaptive ability in adversity to keep normal metabolism． Starch content in tomato
    leaf increased with less water supply．similar to total carbohydrate change． Relationship of“sink—source’’was
    altered under different water supply status．Less water supply made NRA (Nitrate activity)increase signifi—
    cantly，yet which could not retrieve N loss due to water deficit．C
    ， N metabolism was Begative eorrelati0n．
    and C／N ratio increased with water supply decreased，while development degree showed delayed trend
    ． The
    first anthesis， sum of earlier three anthotaxy flowers
    ． and earlier stage yield of the treatment 0f 75％ RWC
    floor level seedlings were better than which of the treatment of 90％ RWC floor leve1． n0 significallt difference
    between the above two treatments，SO 75％ floor level of wate r·supply could be as referenee for potting seed．
    1ings irrigation．
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  • Comparison of the Photosynthetic Characteristics in Four W ild Primula Species
  • Liu Feihu;Liang Xueni;Liu Xiaoli
  • Acta Horticulturae Sinica. 2004, 31(4): 482-486.
  • Abstract ( 1439 ) HTML ( 1227 ) PDF (214KB) ( 1227 ) 　　　

  • P1ants of four wild Pr／mula species were planted in the field at Kunming and the photosynthesis
    was tested with LI-6400 portable photosynthesis system for adding knowledge of photosynthesis to the wild
    Pnm~a species．Species diference Was found in light compensation point(IJCP，txmol’m一’s )，light
    saturation point(LSP，p,mol·m一。s )and CO2 compensation point(CCP，C02p,mol·mol )of photosynthesis．
    Pr／mula pulverulenta Duthie had the highest LCP(28)，the other three species had lower LCP(8—1 5)．
    P obconica Hance had both lOW I5P(80)and photosynthetic rate(Pn)，and highest CCP(80)．P bulleyana
    Fort．and P．pulverulenta showed both the highest I5P(500)and Pn．and intermediate CCP(64—69)，while
    P forrestii Balf．f．had middle LSP and Pn．but the lowest CCP(54)．The diurnal changes of Pn and transpi—
    ration rate(Tr)in May were one．peaked curves．reaching a maximum at about 10 and 13 O’clock for Pn and
    Tr．respectively．The species differences were exhibited by curve height of their diurnal courses

  • Related Articles | Metrics
  • Initiation and Development of the Flower Bud in Zingiber zerumbet
  • Fan Yanping;He Qingtan;Zhao Sheng;Yu Rangcai;and Wu Hong
  • Acta Horticulturae Sinica. 2004, 31(4): 491-495.
  • Abstract ( 1494 ) HTML ( 1462 ) PDF (909KB) ( 1462 ) 　　　
  • The flower bud differentiation process of Zingiber zerumbet were studied by the method of par—
    affin cut and scanning electron microscope．The results showed that the flower bud differentiation process las—
    ted for more than one month from the start of infloreseence pfimordia differentiation in late April to carpel pri—
    mordia formation in the mid of June under the Guangzhou climate condition．The process could be divided into
    9 phases：infloreseenee pfimordium differentiation phase．bract differentiation phase。flower diferentiation
    phase，bracteal leaf differentiation phase，sepal differentiation phase，petal differentiation phase， stamen differentiation
    phase，pistil differentiation phase。anther and ovule differentiation phase．Flowers of inflorescen(：e
    were acropetally initiated and differentiated．
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研究报告

  • Changes of Cell W all and Hydrolases in the Fruit of Two Cultivars of Feicheng Peach during Development and Ripening
  • Xue Bingye and Shu Huairui
  • Acta Horticulturae Sinica. 2004, 31(4): 499-501.
  • Abstract ( 1408 ) HTML ( 1385 ) PDF (113KB) ( 1385 ) 　　　
  • In the process of Feicheng peach Prunus persica(L．)Batsch ev．Feicheng]fruit ripening，
    the proportion of cell wall to fruit mass decreased．Soluble pectins increased remarkably from July 31 to August
    16，leading to fruit firmness decrease． The ionieally bound pectin remained stable in ‘Baili Feiehengtao’
    peach，but it decreased in ‘Hongli Feiehengtao’peach．Decrease of cellulose content in cell wall also played
    an important role in the reduction of firmness．Both PME and cellulases played important role in peach fruit soltening．
    Th e results from the two cuhivars implicated that eellulase is more important in fruit softening．Endo·PG
    had not been detected in ‘Feicheng’ peach．Exo·PG took part in the process of later stage of ripening．
  • Related Articles | Metrics
  • Studies on Phenolic Composition and Polyphenol Oxidase Activity in Apple Fruits
  • Nie Lanchun;Sun Jianshe;Xin Bei;Lu Xinqiong
  • Acta Horticulturae Sinica. 2004, 31(4): 502-504.
  • Abstract ( 2026 ) HTML ( 1809 ) PDF (137KB) ( 1809 ) 　　　
  • The browning degree，content of chlorogenic acid， catechin， epicatechin and polyphenol oxidase(PPO)activity with different substrates in fruits of five apple cuhivars were studied．The results indicated that the fruit with the highest browning degree was‘Fuji’apple llowed by ‘Starkrimson’．‘Orinn’．‘Jonagold’and‘Golden Delicious’．The total content of these three phenolic compounds was the highest in Starkrimson and Fuji apple fruits，and lowest in Golden Delicious．Catechin was deteched only in Jonagold and Golden Delicious fruits．Chlorogenic acid content was distinctively higher than epicatechin in Fuji apple fruit，while epicatechin higher than chlorogenic acid in Starkrimson and Orinn fruit．Taking chlorogenic acid as substrate，the fruit with the highest PPO activity was Starkrimson followed by Fuji and Golden
    Delicious．Taking epicatechin as substrate，Fuji，Jonagold and Orinn fruits had higher PPO activity than Golden Delicious and Starkrimson．Taking catechin as substrate，PPO activity of Orin fruit was the highest followed by Fuji，and Starkrimson was the lowest．
  • Related Articles | Metrics

研究简报

研究报告

  • Leaf Disc Assay for Identification of Resistance of Strawberry to PowderyMildew and Screening of Fungicides
  • Zhang Zhihong;Uu Yan;Gao Xiuyan;Du Guodong;and Dai Hongyan
  • Acta Horticulturae Sinica. 2004, 31(4): 505-508.
  • Abstract ( 1636 ) HTML ( 1724 ) PDF (319KB) ( 1724 ) 　　　
  • The strawberry leaf discs， diameter 1 am ，were inoculated with the conidia of strawberry
    powdery mildew (Sphaerotheca macular~)by spraying or shaking of，and then were transferred to Petri
    plates containing wet filter paper． The leaf discs were cultured at 20℃ with a 1 6 h photoperiod at a light intensity
    of 30O0 lX． The colonies on the inoculated leaf discs were observed clearly 7 days after inoculation．
    The resistance of detached leaf discs of strawberry cultivars ‘Toyonoka’． ‘Akihime’ and ‘Allstar’ to pow—
    dery mildew was consistent with the behavior of these cuhivars in field． The result of screening of fungicides
    by leaf disc assay showed that flusilazol and myclobutanil were effective fungicides for control of powdery rail—
    dew on strawberry．
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  • Heredity and Expression of Foreign Genes in pinⅡ Transgenic FloweringChinese Cabbage Obtained by Vacuum Infiltration
  • Xu Hengjian ’;Liu Fan;Wang Xiufeng;Zhao Hong;Cao Chuanzeng;and Luo Chen
  • Acta Horticulturae Sinica. 2004, 31(4): 511-513.
  • Abstract ( 1464 ) HTML ( 1300 ) PDF (760KB) ( 1300 ) 　　　
  • Transgenic Chinese cabbage (Brassica campestris L．ssp．chinensis var．utilis Tsen et Lee)
    with pin n gene and bar gene were got by vacuum infiltration transformation and strictly self-crossed．PCR assay，
    Basta resistance and insect resistance of T2 generation lines proved stable inheritance and expression of the
    foreign genes．The segregation ratios of 41 lines indicated low—copy transformation，and majority of them accord—
    ing with the Mendel’S segregation law．The resistance of transgenic lines to Plutella xylostella L．has significant
    diference along with the variation of relative quantities of PIN II protein．even lines with the san3．e insertion copies．
  • Related Articles | Metrics
  • The Stress-related Responses of Tomato and Expression of Nr Gene Caused by Ethephon and Abscisic Acid
  • Yang Jinghui;Liu Yanjun;Luo Yunbo;Zhang Weiyu;Yang Enqin;Liu Yudong
  • Acta Horticulturae Sinica. 2004, 31(4): 514-516.
  • Abstract ( 1454 ) HTML ( 1792 ) PDF (463KB) ( 1792 ) 　　　
  • In order to understand the relationship between the ethylene receptor Nr gene and plant
    resistance．the metabolism of tomato plants under stress of ethephon and abcisic acid(ABA)was studied．
    The result showed that ABA induced largely the expression of the Nr gene．This expression was repressed by
    silver ion，an ethylene depressor．ABA improved a triple response of tomato seedling—like efect caused by eth—
    ylene．This decreased respiration and caused leaved chlorosis．The 50 ixmol／L ethephon supplied by spraying
    leaves did not induce the expression of th Nr gene．Although the transpiration rate and stomatal conductance
    were increased，the respiration and calculated CO2 and the plants grow did not change．Thus，overexpression
    of the Nr gene was caused by environmental stress related to ABA．This stress induced the biosynthesis of
    inner ethylene，which is the main reason of leaf chlorosis and plants senescence．
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  • Cloning of Potato Virus S Coat Protein Gene and Its Expression in E．coliJM 109
  • Acta Horticulturae Sinica. 2004, 31(4): 517-519.
  • Abstract ( 1889 ) HTML ( 1214 ) PDF (392KB) ( 1214 ) 　　　
  • Total RNA of potato virus S (PVS)was isolated from diseased potato leaves collected in
    field，The cDNA encoding PVS coat protein(PVS-cp)was amplified by RT-PCR and cloned into the plasmid
    pGEM—T vector． The expression vector of P 一cp gene was constructed through inserting the cDNA fragment
    into the expression plasmid pBV220． The results of SDS-PAGE and W estern blot analysis showed that
    the P -cp gene highly expressed in E．coli JM109 and the molecular size of the expression product was about
    33 kD． The product was used as antigen to immunize rabbits and the specific anti-serum can be used to detect
    PVS rapidly．
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  • Studies on Cultivar Classification of Osmanthus fragrans by ISSR·PCR Analysis
  • Qiu Yingxiong;Hu Shaoqing;Chen Yuelei;Chen Xiao;and Wu Guanghong
  • Acta Horticulturae Sinica. 2004, 31(4): 529-532.
  • Abstract ( 1457 ) HTML ( 1515 ) PDF (460KB) ( 1515 ) 　　　
  • In this study，inter．simple sequence repeat(ISSR)was evaluated for its potential use in the
    identification of 1 9 Osmanthus fragran,s cuhivars．1 3 out of 74(1 8．6％)ISSR primers could generate repro．
    ducible polymorphic fragments．The ISSR．PCR assay revealed a total number of 90 DNA bands．of which 79
    bands were polymorphic(the percentage of polymorphic bands，PPB=87．8％)．Optimized ISSR primers
    amplified 4 to 1 0 bands ranging in size from 300 bp to 2000 bp，with an overall average of 6．92 amplified
    bands per primer．RAPDistance software was used to calculate the Nei’S genetic distance and a dendrogram
    was constructed based on UPGMA cluster analysis．These 1 9 Osmanthus fragrans cuhivars surveyed were clas．
    sifted into eight major groups，and each cuhivar in this study could be distinguished from others．The genetic
    relationship of four cuhivars group and classification of Osmanthus fragrans were also discussed．
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研究简报

研究报告

  • Establishment of Plant Regeneration from Leaves Explants of Rosa hybrida
  • Ren Guifang;Wang Jianhong;Feng Hui;Li Yi;Li Yan;and Shi Xuebo
  • Acta Horticulturae Sinica. 2004, 31(4): 533-536.
  • Abstract ( 1912 ) HTML ( 1590 ) PDF (323KB) ( 1590 ) 　　　
  • The regeneration system of 24 rose cultivars(Rosa hybrida)was researched．The research
    results indicted that genotypes．hormone varieties and its concentrations．cultural time in the dark and ages of
    the explants have great effect on the regeneration of rose leaf explants、Culturing in dark and using tender ex—
    plants can improve the shoot regeneration of leaf explants．Only l 2 varieties can forlTl adventitious buds from
    24 varieties of roses researched． After 30 days of dark culturing，the regeneration rate of Iceberg improved
    from 15．6％ to 45．6％ ．In vitro the regeneration rate of Iceberg，if leaves from upside of rose。iS 46．5％ ：
    from medium is 12．9％ ：from downside is 0、The appropriate media for regeneration from leaf explants of rose
    is MS+6．BA 6、0 mg·L一 +ZT 6．0 mg ·L一 +IBA 0．4 mg·L一 ．The medium for shoot buds elongation iS
    MS+6一BA 2．0 mg·L一 +IBA 0．1 mg·L一 ．The medium for regenerating adventitious roots iS 1／2 MS+
    NAA 0．2 mg·L +0．1％ activate carbon．
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  • Seed Germination in Vitro of Paphiopedilum armeniacum and P．micranthum
  • Chen Zhilin;Ye Xiulin;Liang Chengye;and Duan Jun
  • Acta Horticulturae Sinica. 2004, 31(4): 540-542.
  • Abstract ( 1770 ) HTML ( 1427 ) PDF (277KB) ( 1427 ) 　　　
  • The seeds take from capsules harvested 1 20 days and 1 80 days after pollination of Paphiopedi—
    lum armeniacum and P．micranthum．These were inoculated on 1／4MS， MS， R， KC，VW and “Hyponex
    1” media．AU the germination was recorded in 120 days after pollination for both two species．The higher
    germination rate get in lower salt concentration medium than higher salt concentration medium．R medium iS
    the best for germ ination in test．Coconut milk stimulate germination．The addition of activated charcoal 2 g·
    L‘。and banana homogenate 100 g·L iS benefit for growth and development of seedlings．
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综述

  • Ethylene Receptor and Its Regulation in Ripening Fruits
  • W ei Shaochong;Chen Kunsongh;and Luo Yunbo
  • Acta Horticulturae Sinica. 2004, 31(4): 543-548.
  • Abstract ( 1700 ) HTML ( 2295 ) PDF (277KB) ( 2295 ) 　　　
  • The action of plant hormone ethylene is finally realized through ethylene receptor and its sig—
    nal transduction．The latest advances in researches on the ethylene receptor and fruit ripening is reviewed in
    the present paper．The main contents include：the ethylene receptor and its signal transduction in Arabidopsis，
    and the expression of ethylene receptors in relation to their functions in ripening fruits．Meanwhile，the pros—
    pect of ethylene receptor is predicted as wel1．
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  • Recent Advance in Genus Hosta Study
  • Zhang Jinzheng;Shi Aiping 一;Sun Guofeng;Shi Lei;and Zhang Qixiang
  • Acta Horticulturae Sinica. 2004, 31(4): 549-554.
  • Abstract ( 1458 ) HTML ( 2550 ) PDF (317KB) ( 2550 ) 　　　
  • The genus Hosta plants which are regarded as the most important ornamental plants have been
    made great progress on sciences reach，cultivation and application of horticulture in the world，while Chinese
    people have done little work on these subjects．In order to push ahead of the plaintain lilies development of
    study and application in China，we have reviewed the advances of genus Hosta，studying in respects of evolu—
    tion and taxonomy，cytology，horticulture，heredity in this paper． By analyzing a vast amount of data，the
    direction and emphasis of the research ahead in China are briefly stated．
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新品种

  • ‘Yan An 2’，A New Pear Variety
  • Qi Yongshun;Liu Jin;Song Jinyao;Gao Haisheng;Dong Cuntian;and Xiang Dianfang
  • Acta Horticulturae Sinica. 2004, 31(4): 555-555.
  • Abstract ( 1311 ) HTML ( 939 ) PDF (52KB) ( 939 ) 　　　

  • ‘Yan An 2’is a new pear variety，selected from the colony of(Pyrus ussuriensis Maxim．)
    in Yanshan．The soluble solids content is 1 5．7％ ，the titratable acid content is 1．0 1％ ，and the vitamin C
    content is 9．72 mg。kg～．The culture has an average fruit mass of 103．3 g and matured in late September to
    early October in Qinhuangdao．It’S flesh is fine and tender in texture with the aroma and flavor of Anli．

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  • A New Peach Variety ‘Lihong’
  • Guo Shouhua;Qi Yongshun;Qi Xiudong;Zhao Xianglan;and Shi Mingjing
  • Acta Horticulturae Sinica. 2004, 31(4): 558-558.
  • Abstract ( 1410 ) HTML ( 949 ) PDF (50KB) ( 949 ) 　　　
  • ‘Lihong’is a new early—middle-maturiy peach variety
    ， selected from the bud mutation of‘Jn
    Zaosheng’．The fruit has a round shape with a fresh red peel，good quality， and store up．enduring and ship—endur．
    ing characteristics．Th e average fruit mass is 251 g，with Max．462 g．and the soluble solids content is 12． 5％ ．
    Fruit ripens in early-middle July in Qinhuangdao area．It can be grown in suitable areas of peach cultivations．
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  • A New Sweet Orange Cultivar ‘Tarroco New Line’
  • Jiang Dong;Hong Qibin;Gong Guizhi;Li Xiqing;and Ye Qingliang
  • Acta Horticulturae Sinica. 2004, 31(4): 559-559.
  • Abstract ( 1785 ) HTML ( 1292 ) PDF (61KB) ( 1292 ) 　　　
  • ‘Tarroco New Line’ is a nucellar selection from Tarroco blood orange which originated in
    Italy． The strong growth habit of tree and seedless fruit are similar with Tarroeo
    ． but the fruit size is larger
    than Tarroeo with 1 80 —250 g in weight and the fruit internal quality is excellent． trees perform well with
    characteristic of late maturing，good yield，high resistance to disease and easy management in field． ‘Tarroeo
    New LiHe’ is an excellent late．season sweet orange suitable for flesh consumption．
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  • ‘E M ihoutao 2’——A Premature New Kiwifruit Cultivar with Yellow Flesh
  • Chen Qinghong and GU Xia
  • Acta Horticulturae Sinica. 2004, 31(4): 560-560.
  • Abstract ( 1355 ) HTML ( 921 ) PDF (50KB) ( 921 ) 　　　
  • ‘E Mihoutao 2’ iS a new cuhivar of Actinidia Chinensis
    ． selected from wild kiwifruit．Its aver—
    age fruit mass is 80 g and the biggest one can reaches 135 g．The fruit is premature．yellow flesh． Its fruit is fine
    and tender in texture with a thick sweet taste and rich refreshing aroma． ‘E Mihoutao 2’ fruit has a soluble sol—
    ids content of 14．3％ ～15．2％ ，and the vitamin C content of the fruit is 654．8～939．0 mg／kg pulp．It matures
    matures in mid to late August in Wuhan and its fruit can be stored for about 10～15 days at room temperature．
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  • ‘Nongmei’一A New Variety of P，zfnus communis Fritsch
  • Mei Lixin;Guo Chunhui;Zhang Tan;and Liu Linqiang
  • Acta Horticulturae Sinica. 2004, 31(4): 561-561.
  • Abstract ( 1292 ) HTML ( 1034 ) PDF (55KB) ( 1034 ) 　　　
  • ‘Nongmei’iS a new almond variety introduced from the United States of American． The mul—
    tiple spots regional trials have showed that it has high marketable quality with thin shell
    ， higher ratio of ker—
    nel，neater of nut．It also has many other advantages such as early bearing
    ， high yield，early maturity and SO
    on．‘Nongmei’is suitable f0r growing in north China and northwest of China with cool temperature(≥1 1 oC)
    and less rainfa11．
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  • A New Small—type W atermelon ‘Yuyi Xiuzhen Hongbao’
  • Li Jianwu;Ma Changsheng;Sun Zhongwei;and Cai Xiao
  • Acta Horticulturae Sinica. 2004, 31(4): 562-562.
  • Abstract ( 1762 ) HTML ( 1095 ) PDF (57KB) ( 1095 ) 　　　
  • ‘Yuyi Xiuzhen Hongbgo’is a small—type watermelon hybrid produced by Henan Yuyi Seed
    Company Ltd．． The variety is suitable for planting in greenhouse and open field in some areas in China． The
    lire history is about 85 days．A plant produces 2—3 fruits which mass about 2 kg／fruit． The yield is about
    37．5—45 t／hm ． The fruit is oval in shape with smooth dark—green stripes on its surface． The content of
    soluble solid is about 1 3．3％ o The variety tastes very delicious．It is resistant to diseases and very convenient
    to store and transport．
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  • A New Cultivar of Chinese Cabbage—— ‘Qinza 1’
  • Zhao Limin;Ke Guilan;Song Yanzhi;and Ma Jianxiang
  • Acta Horticulturae Sinica. 2004, 31(4): 563-563.
  • Abstract ( 1293 ) HTML ( 1017 ) PDF (51KB) ( 1017 ) 　　　
  • ‘Qinza 1’is a latest cultivar of Chinese cabbage．It was bred with two self—incompatibility
    line 02S95 and 02S102 from Vegetable and Flower Institute of Horticultural College of NWUAF by reciprocal
    cross． Its core is bomb—shaped with light—green leaf and leaf sheath iS white． The rate of clear vegetable is
    78％ ． Weight of simple core is about 2．5—3．0 kg． It is high resistance to virus， downy mildew，wet rot
    and black rot．
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  • ‘Jinpeng 1’一A New Tomato Hybrid Cultivar Sepecial for Protected Cultivation
  • Li Xiaodong
  • Acta Horticulturae Sinica. 2004, 31(4): 564-564.
  • Abstract ( 2965 ) HTML ( 952 ) PDF (55KB) ( 952 ) 　　　
  • ‘Jinpeng 1’is a new tomato hybrid cultivar special for protected cultivation．It grows inter minably and has spare foliage．Fruit looks pink，highly round without green shoulder，and has big rigidity (0．81 ks／cm )．It has good character for storage and transportation，and high resistance to ToMV，middle
    resistance to CMV．It iS also resistant to leaf mold and Fusarium wilt．and has few stem rot．The cuhivar matures earlier and has high yield during early harvest． ‘Jinpeng 1’is suitable to protective cultivation in both early spring and late autumn．
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  • A Choice Artificial Stuffing and Field Planting Jew’S-ear Variety——‘Shaan’er 3’
  • Li Shengyin;Chang Yangsheng;Hu Caixia;Li Menglou;Yao Junsheng;Li Minglei
  • Acta Horticulturae Sinica. 2004, 31(4): 565-565.
  • Abstract ( 1840 ) HTML ( 1059 ) PDF (55KB) ( 1059 ) 　　　
  • ‘Shaan’er 3’is a excellent variety of jew’s-ear，whose parents are wild N8and IJ78 collected，separated and crossed with multi-spore．It takes 120 days from inoculating to pick done in artificial stuffing and field planting．The fresh ear is dark brown，thick piece．its shape looks like mum with COCks comb． The largest cluster weighs 80 gram per，the root seat is smal1．The dry ear is puce． its expanded degree equals 1：10．The yield is 7．65 t／hm with 20 am ×56 am ×0．05 am plastic bags． Th e variety is resistant to infection．diseases，melting and has good quality，steady high yield，delicious taste．
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