果树

• Genetic Diversity of Morphological Traits in Wild Populations of Castanea mollissima Blume
• MA Yu-min;CHEN Xue-sen;HE Tian-ming;WU Chuan-jin;WANG Na
• Acta Horticulturae Sinica. 2008, 35(12): 1717-1726.
• Abstract ( 2205 ) HTML ( 1809 ) PDF (731KB) ( 1809 ) 　　　
• The diversity of leaf, petiole and nut in 3 wild intraspecific populations (the Qinling Mountains ecotype, the Taiyi Mountains ecotype, and the Yanshan Mountains ecotype) of Castanea mollissima Blume were investigated to provide the scientific basis for resource protection and utilization. The results indicated: ①There were significant difference among and within population. The morphological traits, such as leaf length, width, leaf shape index, length of petiole and nut shape, size, color, have great morphological diversity with variation coefficient (CV) of more than 10%. The range of nut weight varied from 1.69g to 3.89g in Qinling population with CV 18.3%. The range and CV of leaf area and nut weight were the largest, and those of leaf shape index were the smallest; ②The content, of main nutrition composition (water, total sugar, starch, protein, fat, ash and Vc) in ripe nuts determined in obtained 118 seedlings at the Qinling Mountains, Taiyi Mountains and Yanshan Mountains was analyzed and presented considerable genetic variations with CV of 6.2％-28.3％, and the range and CV of the protein were the largest. There were the same trends in the 3 wild intraspecific populations; ③There was significant difference between site conditions, with smallest CV in leaf area and nut weight and highest CV of Vc content; ④Correlation analysis revealed that mean weight of a single nut with annual rainfall and the longitude of each ecotype, in contrast, they were negatively correlated with the altitude. Vitamin C contents in the nuts from different ecotype were negatively regulated with annul rainfall. These relationships indicated that environmental conditions had impact on genetic diversity in Chinese wild chestnut.
  
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• Changes of Soil Nutrition in Root Zone and Their Effects on Growth of the Replanted Apple
• FAN Hong-ke;ZHAO Zheng-yang;LIU Huai-feng;ZHAO Guo-dong;ZHANG Xiao-cai;and ZHANG Zhi-min
• Acta Horticulturae Sinica. 2008, 35(12): 1727-1734.
• Abstract ( 2175 ) HTML ( 1422 ) PDF (833KB) ( 1422 ) 　　　

• Soil nutrient distribution and imbalance were studied in the root zone of 0～80 cm layer and far from the trunk 0-200 cm in Weibei highland orchard, while the replant diseases of potted apple plants were analyzed. Results indicated that soil nutrients advanced along with increasing of the tree root base distance in level distribution；In vertical direction，other soil nutrients dropped along with deepening of the soil layer except for available Ca and Mg. Available P, Ca, Mg, Zn, Mn were deficit in orchard soils and N/P, K/P, Ca/P, Ca/Mg, Ca/Mn, Ca/Zn were imbalance in the root zone. The average of replant plant height, shoot length and rough, leaf area and root-activity were reduced 44.93%，34.46%，23.88%，49.48% and 11.40% separately, which represented replant disease evidently, and which aggravated follow comply with deepening of the layer and lighten as far from the tree trunk in orchard. To sum up, deficiency of Zn and imbalance of Ca/Zn were one of main factors to cause replant diseases; The replant diseases were lighter relatively in the zone of 0～40 cm layer and far from the trunk 100-200 cm, where Soil nutrients were concentrated, which will provide some reference to solve replant diseases in old orchard.
  

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• Mechanism of Anthocyanins and Flavonols in Fruit Development of Strawberries
• ZHANG Qiong;WANG Hong-qing;LENG Ping;JIA Le-xin
• Acta Horticulturae Sinica. 2008, 35(12): 1735-1742.
• Abstract ( 2251 ) HTML ( 2344 ) PDF (620KB) ( 2344 ) 　　　

• ‘Selva’ strawberries (Fragaria×ananassa) were used to study the effects of the mechanism of anthocyanins and flavonols. 12 anthocyanins and 7 flavonols were detected in 'Selva' fruits. The results showed that no anthocyanins were accumulated while abundant flavonols were synthesized in young fruits 15 to 25 days after full bloom. Thereafter, the anthocyanins increased continuously with the fruit development. On the contrary, the content of flavonols in fruits was decreased. Shading reduced fruit anthocyanins and flavonols by 70%~90%, particularly cyanins and quercitrins by 90%. These results may imply that the accumulation of anthocyanins and flavonols were restrained by shading in strawberry, and the light effects on cyanidin and quercetin were greater than on pelargonidin and kaempferol. Two major biosynthates were found in the pathway of accumulating anthocyanins, they were dihydroquercetin at the stage of young fruit and pelargonidin at the stage of mature fruit.
  

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研究简报

• Pollen viability of dimorphic anthers in Lagerstroemia indica
• ZHANG Qin-ying;LUO Feng-xia;LIU Li;and GUO Fu-chang
• Acta Horticulturae Sinica. 2008, 35(12): 1741-1741.
• Abstract ( 1753 ) HTML ( 1458 ) PDF (140KB) ( 1458 ) 　　　
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果树

• Genetic Diversity of Poncirus and Its Phylogenetic Relationships with Relatives as Revealed by Nuclear and Chloroplast SSR
• GONG Gui-zhi;HONG Qi-bin;PENG Zhu-chun;JIANG Dong;and XIANG Su-qiong
• Acta Horticulturae Sinica. 2008, 35(12): 1742-1750.
• Abstract ( 2073 ) HTML ( 2078 ) PDF (739KB) ( 2078 ) 　　　

• 28 accessions of trifoliate orange (Poncirus trifoliata Raf.), hybrids and seven accessions of its relatives, were analyzed for genetic diversity and phylogenetic relationship using nuclear and chloroplast SSR markers. Average PIC (Polymorphism Information Content) value of 0.51 and average expected heterozygosity of 0.52 were given according to nuclear SSR results. 22 common trifoliate orange accessions could be divided into four groups with a genetic distance of 0.16. It indicates a rich genetic diversity of China trifoliate orange germplasm. cpSSR revealed no difference among common trifoliate orange accessions which indicates a relatively conservative chloroplast genome. Four cpSSR loci were found different between Fumin（P. polyandra S. Q. Ding et al.）and common trifoliate orange. Fumin trifoliate orange is genetically distant from common trifoliate orange, from its hybrids as well as relatives. It therefore could be regarded as a true species. The combined application of nuclear and chloroplast SSR could serve a more accurate way to identify trifoliate orange hybrids.
  

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• Characterization of Malate Dehydrogenase Gene from Citrus junos and Its Transgenic Tobacco's Tolerance to Aluminium Toxicity
• ZHANG Mi;LUO Xiao-ying;BAI Wen-qin;LI Yi-ran;HOU Lei;PEI Yan;LI Zhong-wang;and LI De-mou
• Acta Horticulturae Sinica. 2008, 35(12): 1751-1758.
• Abstract ( 2574 ) HTML ( 1912 ) PDF (1297KB) ( 1912 ) 　　　

• Malate, ubiquitously existing in plant, plays a crucial role in various metabolic pathways, including respiration, nitrogen fixation, phosphorus acquisition, and aluminum tolerance. Malate dehydrogenase（MDH） catalyzes the interconversion of oxaloacetate and malate. Previous results suggested that overexpressing MDH gene transgenic plants elevated tolerance to aluminum toxicity. Citrus junos, one of main rootstocks of citrus fruits, is widely used in the Southern of China. As most of soils are acid in the south, to investigate the mechanism of tolerance to aluminum in citrus plants, a clone referred CjMDH (Genbank Accession No. ABI75147) was isolated from the cDNA library of root from Citrus junos cv.Ziyang. CjMDH has an open reading frame (ORF) of 1 269 bp encoding a putative protein with 412 amino acid residues, which shows high identity to other MDHs in plants. The deduced protein contains a NAD binding site and eight malate binding sites. Chloroplast transit pepite is found in N-terminal of CjMDH through iPSORT software. BlastP analysis result reveals over 80% identity and 85% similarity with Arabidopsis thaliana, Nicotiana tabacum, Glycine max, Medicago sativa. Amino acids comparison between CjMDH and other plants MDH reveals highly identity in NAD and malate binding site. Southern blot suggested that CjMDH is a single copy gene in Citrus junos genome. Northern blot and Real time RT-PCR data indicated that CjMDH expressed strongly in root and leaf, especially highest in root than leaf and stem. Subsequently, the CjMDH was constructed into over-expression vector driven by constitutive CaMV35S promoter. The construction was introduced into tobacco mediated by Agrobacterium tumerfaciens, the overexpression transgenic tobacco lines were obtained through Northern blot. Transgenic lines increased Al-tolerance in hydroponic culture, providing evidence that MDH is a potential Al-tolerance gene for plant in acid soils.

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蔬菜

• Study on the Methods of Constructing Chinese Cabbage Core Germplasm Based on the Morphological Data
• LI Guo-qiang;LI Xi-xiang;SHEN Di;WANG Hai-ping;SONG Jiang-ping;and QIU Yang
• Acta Horticulturae Sinica. 2008, 35(12): 1759-1766.
• Abstract ( 2041 ) HTML ( 2056 ) PDF (685KB) ( 2056 ) 　　　

• The method to construct Chinese Cabbage core germplasm based on four types of sampling proportion methods in group, six overall sampling scales and two sampling methods were studied in light of 1 651 accessions of Chinese cabbage germplasm and their data of 43 morphological characters in the national medium-term genebank of vegetable germplasm resources. The main results were as follows: The best proper sampling proportion within group was based on index of genetic diversity which enabled the sampled number or proportion from different groups tend to balance, and better maintained the variability of original collection .The index of genetic diversity of the core germplasm established according to the method of genetic diversity index proportion reached maximum and the ratio of phenotypic retained reached 98% when the overall sampling scale increased to 15%. Although the proportion of phenotypes retained nearly 100% and phenotypic variability changed little, the genetic diversity index of the core germplasm decreased accordingly when the overall sampling scale increased to over 20%. So 15% of the overall sampling sizes were more appropriate. In certain sampling proportion method and sampling scale, I, RPR and CV of the core germplasm constructed by cluster sampling was much higher than that by random sampling. Based on the optimized sampling scheme, the Chinese cabbage core gernplasm of 248 accessions of Chinese cabbage gernplasm were established.
  

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• Effects of Infection with Meloidogyne incognita on Reactive Oxygen Metabolism and Correlated Enzyme Activities in Roots of Eggplant Rootstock Seedlings
• XU Xiao-ming;YU Qin;XU Kun;DONG Can-xing;and WANG Yu-guang
• Acta Horticulturae Sinica. 2008, 35(12): 1767-1772.
• Abstract ( 1978 ) HTML ( 1662 ) PDF (569KB) ( 1662 ) 　　　
• The effects of nematode infection on reactive oxygen metabolism and the activities of related enzymes were investigated and compared between the seedling roots of nematode-resistant eggplant variety Solanum torvum and susceptible Solanum intergriflium in this study. The results showed that the production rate of superoxide anion radical (O₂^-·), the content of hydrogen peroxide (H₂O₂) as well as the activities of superoxide dismutase (SOD), catalase (CAT), chitinase (CHT) and β-1,3-glucanase (GLU) were rather higher in the resistant variety than susceptible one, while peroxidase (POD) activity acted contrarily in resistant and susceptible varieties. At the early stage of first infection, O₂^-·production rate, H₂O₂ content and the activities of CHT and GLU were increased rapidly in both varieties with the increasing rate higher in S. torvum than S. intergriflium. At the late stage of first infection, however, all above four indexes decreased. Interestingly, those indexes enhanced again at the late stage of second infection with a smaller increment than the first infection, especially in susceptible variety S. intergriflium. In addition, the activities of SOD and CAT in roots infected with M. incognita was remarkably decreased with the decreasing rate higher in S .torvum than S. intergriflium at the early stage of infection circle, while their activities were increased in both varieties at the later stage of infection circle. The changes of POD activity were contrary to that of SOD and CAT during the whole infection circle. Infection by M. incognita also led to a persistent increase in malondialdehyde content in roots with S. intergriflium being higher than S. torvum, suggesting that nematode infection aggravated the extent of membrane lipid peroxidation in roots, especially in susceptible variety.
  
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• Genetic analysis and Specific Fragments Linked to Powdery Mildew Resistant Gene Pm-2F in Melon
• Zhang Hai-ying;Su Fang;Guo Shao-gui;Gong Guo-yi;Yi Hong-ping;Wu Ming-zhu;and Xu Yong
• Acta Horticulturae Sinica. 2008, 35(12): 1773-1780.
• Abstract ( 2184 ) HTML ( 1811 ) PDF (636KB) ( 1811 ) 　　　

• Podosphaera xanthii （DC.）VP Gelyuta race 2F caused melon (Cucumis melo L.) powdery mildew was the prevailing race in Beijing. The segregation of resistance had been evaluated in F2S6 population of the cross between the resistant Japanese cantaloupe（Cucumis melo L. ssp. melo.convar. cantalupa (Pang.) Greb.）line K7-1 and the susceptible Xinjiang Hami melon（Cucumis melo L. ssp. melo. convar. ameri (Pang.) Greb.）line K7-2. The resistance to P. xanthii race 2F in K7-1 was controlled by one single dominant Pm-2F gene. SSR technique had been used for the identification of markers linked to powdery mildew resistance. Two specific fragments CMBR120_-172 and CMBR8_-98 were closely linked to Pm-2F gene at a distance of 1 cM and 3 cM, respectively. The efficiency of CMBR120_-172 was about 87.5% in 120 melon germplasms. The markers identified in this experminent were closest to Pm-2F gene to date, and the research, therefore, can be used in melon molecular breeding.
  

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• Induction of Autotetraploid Cucumbers by Unpollinated Ovary Culture and Their Characterization
• DIAO Wei-ping;JIA Yuan-yuan;JIANG Biao;BAO Sheng-you;LOU Qun-feng;and CHEN Jin-feng
• Acta Horticulturae Sinica. 2008, 35(12): 1781-1786.
• Abstract ( 2209 ) HTML ( 1503 ) PDF (576KB) ( 1503 ) 　　　

• Gynogenic embryos originating from unpollinated ovary culture were cultured on differentiation medium to induce homozygous autotetraploid cucumber. In total, thirty-three plantlets were regenerated, and seven were observed as autotetraploid (2n=4x=28) after chromosome counts. These autotetraploid plants were identified as homozygote after simple sequence repeat (SSR) analysis. Genetic stability of the progenies from self-crossing of these autotetraploid was studied, no ploidy variation occurred among these progenies after morphology and chromosome observation. Comparative studies were carried out between autotetraploid plants from ovary culture and autotetraploid plants from colchicines treatment, using parameters i-e, stainability of pollen grains, viability of pollen grains and number of seeds per fruit. Higher pollen fertility and number of seeds per fruit were found in the gynogenic induced autotetraploid plants. These results indicate that it is a method to induce autotetraploid through unpollinated ovary culture, and the obtaining of homozygous autotetraploid with higher fertility will be quite useful in cucumber breeding programs.
  

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观赏植物

• Cloning and Transient Expression Assay of BADH Gene Promoters from Dendronthema lavandulifolium
• LIU Zhen-lin;CAO Hua-wen;XIA Xin-li;YIN Wei-lun;and DAI Si-lan;
• Acta Horticulturae Sinica. 2008, 35(12): 1787-1794.
• Abstract ( 2474 ) HTML ( 2448 ) PDF (1235KB) ( 2448 ) 　　　

• In order to provide inducible promoter for chrysanthemum [Dendronthema ×grandiflora (Ramat.) Kitam.] transgenic breeding, referring to the strategy of 5′RACE, four promoter sequences of betaine aldehyde dehydrogenase (BADH) gene from Dendranthema lavandulifolium (Fisch. ex Trautv.) Makino were cloned by anchored PCR walking, which were named DBP11, DBP12, DBP21 and DBP22 (GenBank accession No. DQ497620~DQ497623). The four sequences are 1 230 bp, 1 249 bp, 1 273 bp and 574 bp long respectively. The homology of the corresponding regions between every two sequences is above 89%. DBP12 and DBP21 are the promoters of DlBADH1 and DlBADH2 (GenBank accession No. DQ011151 and DQ011152), DBP11and DBP22 are the promoters of other members in BADH gene family from Dendranthema lavandulifolium. Many cis-acting elements related to water stress and ABA inducement were found in all the sequences. New expression vectors were constructed by replacing the 35S-CaMV promoter with the above promoter sequences to drive the reporter gene GUSplus of the expression vector pCAMBIA1305.2. The new vectors were transferred into Agrobacterium to infect leaf disks of Dendranthema lavandulifolium. The result of transient expression indicated that all the sequences had the function to drive reporter gene.
  

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• Adjustment of the Status of Embryogenic Callus and Plant Regeneration of Lilium longiflorum
• WANG Jie;LIU Guo-feng;HUANG Li;and BAO Man-zhu
• Acta Horticulturae Sinica. 2008, 35(12): 1795-1802.
• Abstract ( 2406 ) HTML ( 1475 ) PDF (688KB) ( 1475 ) 　　　
• In this study, the effects of different concentrations of CoCl₂, ABA, sucrose and the condition of illumination on the status of embryogenic callus that induced from pseudo-bulblet of Lilium longiflorum was studied，and the regeneration of embryogenic callus was studied from active carbon. The results showed that the proportion of somatic embryos and granularity of the callus could be increased by using CoCl₂ with the concentration of 0.05 μmol·L^-1, while 0.01 μmol·L^-1 and 0.02 μmol·L^-1 CoCl₂ are beneficial to the increasement of callus volume and proliferation coefficient; Some negative effect on growth potential, water content, volume and proliferation coefficient of embryogenic callus was observed when the concentration of ABA was gradually increased, while proportion and granularity of embryogenic callus were not affected by different ABA concentrations; Culturing in light, the positive effects of 6.0 g·L^-1 or 9.0 g·L^-1 sucrose on proportion and granularity of embryogenic callus were observed, while only 6.0 g·L^-1 sucrose has positive effects when cultured at dark; Furthermore, the regeneration of embryogenic callus was affected by 1g/L active carbon and basic medium. Considering all factors in present study, the results demonstrated that the most appropriate condition for the proliferation and regeneration of embryogenic callus were: culture under light, MS + NAA(5.4μM/L) + TDZ(0.4μM/L) + CoC12(0.05μM/L) + source(60g/L) and MS+ active carbon (1g/L) + source(30g/L)。
  
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研究简报

• Cloning of CsNAC Gene from Navel Orange and it's Expression Anlysis during the Storage of Fruits
• FAN Jing;LI Zheng-Guo;GAO Xue;YANG Ying-Wu;and DENG Wei
• Acta Horticulturae Sinica. 2008, 35(12): 1803-1808.
• Abstract ( 1821 ) HTML ( 1630 ) PDF (811KB) ( 1630 ) 　　　
• Based on the cDNA subtraction library which had been constructed to identify differentially expressed genes in peel pitting of citrus fruit, the full-length cDNA sequence of CsNAC homologous to NAC gene family was isolated from navel orange (Citrus sinensis Osbeck) by RACE (rapid amplification of cDNA ends). CsNAC is of 1 203 nucleotides encoding a protein of 305 amino acids. The calculated molecular weight of the CsNAC protein was 35.2 kDa and theoretical isoelectric point was 6.72. Sequence analysis showed that the CsNAC protein had a strikingly conserved region at the N-terminus, which is considered as the characteristic of NAC protein family. Phylogenetic analysis confirmed CsNAC belonged to the ATAF subfamily, which plays an important role in response to stress stimuli. The expression of CsNAC was markedly enhanced in the pitting peel compared to healthy peel during the storage by real-time quantitative PCR, indicating that CsNAC is involved in the development of the peel pitting of navel orange fruits.
  
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• Meiosis of Pollen Mother Cells of Autotetraploid Carrot
• PEI Hong-xia;ZHUANG Fei-yun;OU Cheng-gang;and ZHAO Zhi-wei
• Acta Horticulturae Sinica. 2008, 35(12): 1809-1814.
• Abstract ( 1961 ) HTML ( 1311 ) PDF (542KB) ( 1311 ) 　　　
• Chromosomes behaviors of pollen mother cells (PMCs) of autotetraploid carrot (2n=4x=36) were observed using enzymes digestion technique. There were more than two nucleoli in about 4.58% PMCs at premeiotic interphase and prophaseⅠ. Besides bivalents, univalents and multivalents were observed at diakinesis and metaphaseⅠ. Some chromosomes (about 1 to 6) were found not to rank on the metaphase plate in about 23.0% PMCs at metaphaseⅠand 32.3% PMCs at metaphaseⅡ, respectively. The average chromosome configuration was 0.86Ⅰ+4.01Ⅱ+2.99Ⅲ+1.89Ⅳ+0.81Ⅴ+1.09Ⅵ. Chromosome lagging, chromosome bridge and spindle mis-orientation were also observed. Abnormal chromosome behavior of PMCs was reason for polymorphism of male gametophytes and lower fertility of pollen in autotetraploid carrot.
  
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• Molecular Diagnosis of Tomato Yellow Leaf Curl Disease in Jiangsu Province
• JI Ying-hua;XIONG Ru-yi;CHENG Zhao-bang;ZHOU Tong;ZHAO Tong-min;YU Wen-gui;FAN Yong-jian;and ZHOU Yi-jun
• Acta Horticulturae Sinica. 2008, 35(12): 1815-1818.
• Abstract ( 2216 ) HTML ( 1307 ) PDF (323KB) ( 1307 ) 　　　

• In the winter of 2007, a new virus disease on tomato broke out in Jiangsu and caused great losses to local tomato production. The infected tomato showed severely stunted, upward curling and prominent yellowing along margins and interveinal regions. Based on its symptoms and the occurrence of Bemisia tabaci in the fields, Whitefly-transmitted geminivirus (WTG) was suspected to be the pathogen, and WTG was identified in all the 20 samples. The nucleotide sequence identities between these isolates were extremely high (over 98.9%), which means no complex infection was found. The result of BLAST revealed they shared high sequence identities (over 99%) with a member of Begomovirus: Tomato yellow leaf curl virus (TYLCV).
  

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• Changes in UWL Intensity, Respiration Rate and Physiological Metabolism of Chrysanthemum during Floral Differentiation　　　
• LIN Gui-yu;HUANG Zai-fan;ZHANG Cui-hua;and ZHENG Cheng-shu;
• Acta Horticulturae Sinica. 2008, 35(12): 1819-1824.
• Abstract ( 2086 ) HTML ( 1853 ) PDF (661KB) ( 1853 ) 　　　

• This studies were carried out to investigate dynamic changes in ultraweak luminescence (UWL) intensity, respiration rate, contents of ATP, soluble sugar and soluble protein of chrysanthemum leaves during floral differentiation. The results showed that the UWL intensity increased 119.3%, respiration rate increased 102.4%, contents of ATP, soluble sugar and soluble protein increased 148.6%, 95.5%, 18.3% respectively at initial stage of floral bud differentiation (II) compared with those of vegetative stage of apical bud (I), and then UWL intensity, respiration rate and contents of ATP reduced slowly respectively at stage of involucre primordial differentiation (III), stage of floret primordial differentiation (IV) and stage of crown form (V). Contents of soluble sugar decreased mostly at stage IV and stage V, and the levels neared to those of controls. Contents of soluble protein maintained higher levels at stage II, stage III, and stage IV, decreased mostly at stage V, but it still increased 14.0% compared with those of controls. But all of UWL intensity, respiration rate, and contents of ATP, soluble sugar and soluble protein maintained stable status in all stages of controls of non-short day treatments. This suggested that the UWL has close relation to respiration and energy metabolism of leaves during differentiation of chrysanthemum.
  

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• Morphological Differentiation of Flower Bud of Cattleya labiata
• ZHENG Bao-qiang;WANG Yan;PENG Zhen-hua;and LI Li
• Acta Horticulturae Sinica. 2008, 35(12): 1825-1830.
• Abstract ( 2112 ) HTML ( 1395 ) PDF (1126KB) ( 1395 ) 　　　
• The flower bud differentiation process of Blc.Sung Ya Green‘Green World’ was studied by the method of paraffin cut.The results showed that the flower bud differentiation process lasted for less than three months from the start of inflorescence primordia differentiation in early July to column and pollinia formation in the end of September under the greenhouse climate condition in North China. The process could be divided into 6 phases: undifferentiation phase, inflorescence primordium differentiation phase, flower differentiation phase, sepal differentiation phase, petal differentiation phase, and column and pollinia differentiation phase. The phases of flower differentiation, column and pollinia differentiation were relatively longer. The new plant finished its growth when sepal differentiation phase begun.
  
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• Application of Mixed Substrates on Soilless Culture of Euphorbia pulcherrima
• SUN Xiang-li and ZHANG Qi-xiang
• Acta Horticulturae Sinica. 2008, 35(12): 1831-1836.
• Abstract ( 1742 ) HTML ( 1366 ) PDF (324KB) ( 1366 ) 　　　

• In order to study the reduce of dependence on peat and cost in the soilless culture of flowers, the effects of experimenting different mixing substrates, taking sawdust, corn stalk and wheat-straw as the primary growing media of Euphorbia pulcherrima were investigated. The results indicated that the physical and chemical characteristics of the two treatments V(corn stalk):V(sawdust):V(perlite)2:1:1, and V(wheat-straw):V(sawdust):V(perlite)1:2:1 satisfied the general requirements of growing media. Not only the roots of Euphorbia pulcherrima in those treatments were robust, the comprehensive performance of growth and development were also significantly better than that in control. Thus, the treatments can be good substitutes as soilless growing media of Euphorbia pulcherrima.
  

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• Genetic Relationship of Some Cultivars of Petunia hybrids Using SRAP Marker
• XU Jin;ZHANG Xi-xi;DONG Ai-xiang;WANG Tao;and ZHAO Liang-jun
• Acta Horticulturae Sinica. 2008, 35(12): 1837-1842.
• Abstract ( 1853 ) HTML ( 1279 ) PDF (441KB) ( 1279 ) 　　　
• In this paper, the molecular marker system-SRAP (sequence-related amplified polymorphism) was firstly applied on 58 Petunia hybrids' genetic relationship research, the results obtained was as follows: 20 primer pairs selected from 88 primer pairs amplified 389 polymorphic bands with an average of 19.5 polymorphic bands per primer pair, and the number of bands amplified by each primer pair was ranged from 13 to 40. Moreover, Jaccard's similarity coefficient ranged from 0.55 to 0.87. 58 varieties could be divided into 4 groups when the genetic distance was 0.67. The first group mainly included warm colour serieses, and yellow flower variety was in the second group, while cold colour varieties were clustered in the third and forth group. In addition, the white colour varieties were clustered in a wide range. Study on genetic relationship of Petunia is benefit for variety protection, seed purity testing and genetic breeding.
  
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• Detection and Sequence Analysis of Lily mottle virus in Narcissus pseudonarcissus from the Netherlands by RT-PCR Technique
• LIU Bo;MING Jun;LIU Chun;LUO Feng-xia;WANG Chun-cheng;SHAN Hong-chen;WANG Xiao-wu;and MU Ding
• Acta Horticulturae Sinica. 2008, 35(12): 1843-1848.
• Abstract ( 2199 ) HTML ( 1491 ) PDF (321KB) ( 1491 ) 　　　

• Lily mottle virus（LMoV）was detected in Narcissus pseudonarcissus using one set of specific primer by reverse transcription-polymerase chain reaction (RT-PCR). A 553bp DNA fragment was amplified from the sample of ‘Pink-charm’ using primers L1 (5'-TGGGCACCTTGTGAATTAC-3') and L2 (5'- TGCTGTATGCCTCTCCGTGC-3'). The primer was designed according to the published sequence of the coat protein of LMoV in the GenBank. Nucleotide sequence of the fragment（GenBank Accession No. EU167936）showed more than 98% identity with other isolates（GenBank Accession Nos.:AJ748256；AJ564636；AB053256；AF531458；AJ564637；AJ748257）. Remarkably high Similarity in the nucleotide has been observed despite of their different origins. Besides, amino acid sequence of the amplified virus fragments（GenBank Accession No. ABW16938）is overlapped by the amino acid sequence of LMoV Coat protein（GenBank Accession No .NP-945145）.
  

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新品种

• A New Early-ripening and High-quality Pear Cultivar‘Ganlizao 8’
• WANG Fa-lin;LI Hong-xu;MA Chun-hui;and LI Yin-sheng
• Acta Horticulturae Sinica. 2008, 35(12): 1849-1849.
• Abstract ( 1797 ) HTML ( 1145 ) PDF (380KB) ( 1145 ) 　　　
• ‘Ganlizao 8’is a new pear cultivar which bred from 'Shihyakume' × 'Zaosu' . Its fruit is ovoid in shape, average fruit mass is 256 g. The fruit skin is yellow-green and the flesh is crisp, having little stone cell, abundant juice. The soluble solids content is 12.6%-15.4%.The fruit quality is excellent. The fruit mature in early August in Lanzhou area.
  
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• An Early-Ripening Table Grape Cultivar ‘Jingxiangyu’
• FAN Pei-ge;YANG Mei-rong;WANG Li-jun;LI Sheng-chen;WU Ben-hong;LI Lian-sheng;and LI Shao-hua
• Acta Horticulturae Sinica. 2008, 35(12): 1850-1850.
• Abstract ( 3173 ) HTML ( 1092 ) PDF (73KB) ( 1092 ) 　　　
• ‘Jingxiangyu’is an early- ripening table cultivar derived from ‘Jingxiu’× ‘Xiangfei’. Berries mature during the first 10 days of Augest in Beijing. The fruit cluster is conical and medium-dense, weighing 463.2 g on average with uniformly sized berries. The average mass of a single berry is 8.2 g. The berry is elliptic, yellow- green and difficult to detach from pedicel. The flesh is crisp and slightly juicy with 14.5 %- 15.8 % soluble solid content, 0.61 % titratable acid content and muscat flavor. It has high yield and medium resistance to diseases.
  
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• A New Chestnut Cultivar ‘Yanlong’
• WANG Tong-kun;QI Yong-shun;ZHANG Jing-zheng;and LI Cai
• Acta Horticulturae Sinica. 2008, 35(12): 1851-1851.
• Abstract ( 1829 ) HTML ( 1302 ) PDF (93KB) ( 1302 ) 　　　
• ‘Yanlong’is a new chestnut cultivar selected from the seedling progeny of Yanshan chestnut. Through heredity determination of vegetative progeny and field test, it showed excellent characteristics such as less catkins, high yield, big nut, good color and high quality, and the heading-back trait of stick was suitable for close planting.
  
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• A New Variety of Armeniaca vulgaris×A. sibirica‘Boke 1’
• ZHANG Ling;LI Bing-ren;ZHAI Ming-pu;LI Jian-hong;HOU Zhi-xia;SU Shu-chai;GUO Su-juan;WEI Shu-zhen;and LI Jian-jun
• Acta Horticulturae Sinica. 2008, 35(12): 1852-1852.
• Abstract ( 2155 ) HTML ( 1013 ) PDF (94KB) ( 1013 ) 　　　
• ‘Boke 1’ is a new cultivar which derived from 'Youyi' variety of Armeniaca vulga ris×A. sibirica. It has prominently high yield of average 11.1 kg stone per adult tree, and the ratio of kernel versus stone is 42.35%～45％.The crust of its stone is weak, about 0.9-1.12 mm, and the thickness is relates with its yield, the crust became thinner with the yield increased.
  
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• A New Middle Maturing Wax Gourd F1 Hybrid‘Rongkang 4’
• ZHANG Jian-jun;LIU Shi-gui;ZHAN Cheng-bo;and AO Qing-yan
• Acta Horticulturae Sinica. 2008, 35(12): 1853-1853.
• Abstract ( 2198 ) HTML ( 1040 ) PDF (74KB) ( 1040 ) 　　　
• ‘Rongkang 4’is a new wax gourd F1 hybrid developed by crossing inbred line ‘Q1-05-1’with inbred line ‘RK-1-3-2’.The first female flower node is at 13th－16th.It is heat-resistant and easy to set fruit. It takes about 120 days from sowing to harvesting in early spring .The average fruit weight is 15kg.The fruit tegument is deep green. The flesh is not easy to cook mashed .The fruit is fit for cooking, barbecue, chaffy dish , preserved fruit and suit to long-distance transportation.
  
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• A New High-sulforaphane-content Broccoli Cultivar ‘Fuqing 1’
• HUANG Ke;;;WU Qiu-yun;LI Bin;and ZHENG Jin-gui;
• Acta Horticulturae Sinica. 2008, 35(12): 1854-1854.
• Abstract ( 1899 ) HTML ( 1200 ) PDF (95KB) ( 1200 ) 　　　
• ‘Fuqing 1’is a F1 hybrid with high sulforaphane. It is medium-late. The sulforaphane content of 'Fuqing 1' is 984.47 μg·g-1 and the control is 104.15 μg·g^-1 , the content is 8.45 more than the control. It grows well and the height is 60 cm, the spread is 65 cm with 15 leaves. Green curd, small alabastrum, harvested 80 d after planting.
  
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• A New Siraitia grosvenorii Cultivar ‘Yongqing 1’
• MA Xiao-jun;MO Chang-ming;BAI Long-hua;and FENG Shi-xin
• Acta Horticulturae Sinica. 2008, 35(12): 1855-1855.
• Abstract ( 1814 ) HTML ( 1162 ) PDF (378KB) ( 1162 ) 　　　
• ‘Yongqing 1’ is a new Siraitia grosvenorii cultivar which is derived from the cross of 'Longjiangqingpi' × 'Dongguaguo'. It has excellent characteristics such as nice fruit shape, high yield and adverse resistance. The single fruit weight is long. The big fruit rate is 73.48% and the mogrosidesⅤ content is 1.03%.
  
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