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2016, Vol.43, No.10 Previous Issue    Next Issue
  • Effects of Malus hupehensis Seedlings and Allium fistulosum Mixed Cropping on Replanted Soil Environment
  • LI Jia-jia,XIANG Li,PAN Feng-bing,CHEN Xue-sen,SHEN Xiang,YIN Cheng-miao*,and MAO Zhi-quan*
  • Acta Horticulturae Sinica. 2016, 43(10): 1853-1862. DOI:10.16420/j.issn.0513-353x.2015-0966
  • Abstract ( 326 ) HTML ( 988 ) PDF (727KB) ( 988 )    
  • Effects of Allium fistulosum L. and apple seedlings mixed cropping on the replant soil environment and growth of Malus hupehensis Rehd. seedlings were studied under pot condition. Results showed that mixed cropping enhanced Malus hupeheusis Rehd. seedlings biomass. Plant height,ground diameter,fresh weight and dry weight were 1.42,1.67,3.63 and 2.99 times larger than the control group. The root respiration rate of Malus hupehensis Rehd. seedlings was significantly improved. Activities of antioxidant enzymes,such as superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD)were 1.58,2.74,2.41 and 6.79 times higher than the control group. The activities of soil enzymes including  urease,invertase,phosphatase and catalase were significantly improved. The activities of soil enzymes in mixed cropping were better than in lime nitrogen,and after that was methyl bromide and apple replant in order. Numbers of bacteria and actinomycete were 2.44 and 1.79 times more than the control group and numbers of fungi dropped by 69.4%. The ratio of bacteria to fungi in different soils was as follows:methyl bromide > mixed cropping > lime nitrogen > replanted soil. A. fistulosum L. and apple seedlings mixed cropping significantly improved margalef index,Shannon’s index,pielou index and decreased simpson index. The number of Fusarium oxysporum was reduced significantly compared with the control group. In conclusionA. fistulosum L. and apple seedlings mixed cropping decreased the number of fungi and increased that of bacteria,could reduce apple replant disease.

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  • Changes in Aroma Volatiles of Xinjiang Apricot Fruit During Development and Ripening and Characterization of Key Aroma Components
  • LU Juan-fang1,ZHENG Hui-wen1,ZHENG Qiao1,ZHANG Qiu-yun1,LI Wen-hui2,and XI Wan-peng1,3,*
  • Acta Horticulturae Sinica. 2016, 43(10): 1878-1890. DOI:10.16420/j.issn.0513-353x.2016-0303
  • Abstract ( 295 ) HTML ( 840 ) PDF (1594KB) ( 840 )    
  • Aroma volatiles in fruit of five Xinjiang apricot cultivars were determined by Gas Chromatograph–Mass Spectrometer(GC–MS)method. Totally,154 aroma volatiles were identified from these fruit tested,among them,58 aroma volatiles were the rich compounds. The composition and contents of Xinjiang apricot fruit were significantly regulated by ripening. The content levels of linalool,α-terpineol,geraniol,β-myrcene,ocimene,2-hexenol,phenylacetaldehyde and hexanal decreased remarkably throughout the whole development and ripening period,while the content levels of β-ionone,geranyl acetone,γ-decalactone,δ-decalactone and γ-dodecalactone increased rapidly with fruit ripening. During the early development period,apricot fruit mainly accumulated alcohols,aldehydes,terpenoids and terpenes,but ketones and lactones predominated in fruit at the ripening stage. Significantly higher levels of  terpenoids,terpenes,alcohols,aldehydes and lactones were found in apricot peels than those in pulps,however,significantly lower levels of ketones were found in peels than those in pulps. Based on orthonasal odor thresholds(OOT)method,a total of 22 aroma volatiles were identified as the characteristic aroma components of Xinjiang apricot fruit,linalool,γ-decalactone,γ-dodecalactone,β-damascenone,(E,Z)-2,6-nonadienal,phenylacetaldehyde,hexanal,α-terpineol,hexyl acetate,geraniol and β-myrcene were the most important contributors to aroma quality of Xinjiang apricot fruit.

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  • Identification and Expression Profile of the SnRK2 Family Genes in Grapevine
  • MA Zong-huan,MAO Juan,LI Wen-fang,YANG Shi-mao,WU Jin-hong,and CHEN Bai-hong*
  • Acta Horticulturae Sinica. 2016, 43(10): 1891-1903. DOI:10.16420/j.issn.0513-353x.2015-0912
  • Abstract ( 327 ) HTML ( 804 ) PDF (1758KB) ( 804 )    
  • Full length cDNA sequence of 8 grape SnRK2 genes(VvSnRK2)were cloned from grapevine Ruby Seedless using RT-PCR,sequence analysis showed that the N-terminal domain of VvSnRK2 was highly conserved,while divergent in the C-terminal. The phylogenyetic analysis showed that VvSnRK2s could be divided into three groups. The VvSnRK2s protein carry all acidic amino acids and they all hydrophilic proteins. All VvSnRK2 but VvSnRK2.2VvSnRK2.8 genes carry 9 exons. The predicted secondary structure suggested that the main structure of eight proteins are alpha helix,beta turn and random coil. The subcellular localization were predicted in the cytoplasmic. The analysis of cis-regulatory elements showed that VvSnRK2s contained one or more of ABRE,DRE/CRT,LRTE elements except VvSnRK2.1VvSnRK2.2 and VvSnRK2.6. Quantitative real-time PCR data indicate that VvSnRK2.7 and VvSnRK2.8 were expressed at the highest-level in the roots and stems,which were as 3.8 and 5.0 times as leaves,respectively. Under the 0 and–4 ℃ treatments,VvSnRK2.2 was down-regulated,while the expression was 0 of VvSnRK2.8. Under the 30 treatment,VvSnRK2.2 and VvSnRK2.5 were up-regulated 3.8 and 3.6 folds,respectively. VvSnRK2.1 and VvSnRK2.2 were response to salt stress,while VvSnRK2.5 was highly response to drought stress.

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  • Fruit Character Diversity Analysis and Numerical Taxonomy of Wampee (Clausena lansium)Germplasm Resources
  • LU Yu-sheng,LIN Zhi-xiong,QIU Ji-shui,CHANG Xiao-xiao,and PAN Jian-ping*
  • Acta Horticulturae Sinica. 2016, 43(10): 1903-1915. DOI:10.16420/j.issn.0513-353x.2016-0176;http://www. ahs. ac. cn
  • Abstract ( 196 ) HTML ( 825 ) PDF (763KB) ( 825 )    
  • In order to further understand the genetic diversity of wampee(Clausena lansium)germplasm resources,fruit characters of 96 wampees conserved at the National Depository of Wampee Germplasm(Guangzhou)were evaluated in this study according to the book of‘Descriptors and Data Standard for Wampee[Clausena lansium(Lour.)Skeels]’and the data was numerical classified by Q cluster analysis,R cluster analysis and principal component analysis. The results indicated that the average variation type of 22 descriptive characters was up to 3.4,among which the shape of seed had the most variation with coefficient 6. Among 16 numerical characters,the number of fruits per ear possessed the largest coefficient of variance,which was 58.78%,while the lateral diameter of seed had the least  coefficient of variance,6.34%. The resultof Q cluster analysis showed that 96 wampee germplasm resources were divided into 4 groups at the Euclidean distance of 19.84 based on characters of fruit ear,fruit size and fruit taste. The result of R cluster analysis indicated that 38 fruit characters which show close relationship were significantly clustered into 4 groups at similarity coefficient of 1.45. Principal component analysis suggested that the first and second principal components could explain 28.16% of total variance,and the results were basically consistent with the cluster analysis.

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  • Isolation and Expression Analysis of the Genes Encoding Polar Auxin Transporter AcPINs and AcAUXs in Pineapple
  • LI Yun-he1,2,3,SUN Guang-ming1,ZHANG Hong-na1,LIU Sheng-hui1,and WU Qing-song1,3,*
  • Acta Horticulturae Sinica. 2016, 43(10): 1916-1928. DOI:10.16420/j.issn.0513-353x.2016-0410
  • Abstract ( 441 ) HTML ( 1136 ) PDF (5255KB) ( 1136 )    
  • Exogenous ethylene has been widely used to induce pineapple flowering,but the molecular mechanism behind ethephon induction is still unclear. Three genes encoding auxin efflux carriers (designated as AcPIN1AcPIN2 and AcPIN3),and two genes encoding auxin influx carriers(designated  as AcAUX1 and AcAUX2)were isolated from the pineapple(Ananas comosus L.‘Perola’)shoot apex using RT-PCR and RACE. The full-length cDNA of AcPIN1AcPIN2AcPIN3AcAUX1 and AcAUX2 were 2 690,2 388,2 057,2 156 and 1 580 bp,with the open reading frames of 1 854,1 917,1 530,1 479 and 1 392 bp,which encode a putative protein of 617,638,509,492 and 463 amino acids,respectively. The genomic DNA sequences of AcPIN1AcPIN2AcPIN3AcAUX1 and AcAUX2 were 3 602,3 208,4 204,5 457 and 2 436 bp,with the lengths of 3 244,2 780,3 947,5 264 and 2 321 bp,respectively,from the start codon to the terminator codon. The results of a phylogenetic tree analysis indicated that AcPINs and the AcAUXs belong to plant PINs and AUXs/LAXs groups. Quantitative real-time PCR indicated that the relative expressions of AcPINs were up-regulated much more than AcAUXs after the treatment of 200 and 1 200 mg · L-1 ethephon. The expression of AcPIN2 was significantly increased at the former period(1–2 d)and the later period(28–37 d)after the treatment. In contrast,the expressions of AcPIN1 and AcPIN3 were significantly up-regulated at most time points after the treatment. On the other hand,the up-regulated expression of AcAUX1 was mainly on the former period(1 d)and later period(28–37 d),while the significantly up-regulated expression of AcAUX2 was only found at the former period(1,2 and 9 d after the treatment). The results showed that there were polar auxin transport during the flowering of pineapple,and auxin efflux might play a more important role during this process.

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  • Effects of Exogenous Salicylic Acid on the Expression of CsFAD Genes in Cucumber Seedlings Under Low Temperature
  • CAO Ning,DONG Chun-juan*,and SHANG Qing-mao*
  • Acta Horticulturae Sinica. 2016, 43(10): 1941-1952. DOI:10.16420/j.issn.0513-353x.2016-0250
  • Abstract ( 243 ) HTML ( 618 ) PDF (757KB) ( 618 )    
  • To explore the effects of salicylic acid(SA)on fatty acid compositions in cucumber (Cucumis sativus L.)seedlings under low temperature,the changes in total contents of fatty acids and relative content of each composition,as well as the expression levels of fatty acid desaturase genes(CsFADs)were detected. The results showed that SA application could repress the low temperature- induced increase in malondialdehyde content and relative electrolyte leakages,and maintain the stability of cell membranes. Under low temperature,total contents of fatty acids were decreased. Especially,the relative contents of polyunsaturated fatty acids(e.g. C18:3)were decreased,while the ratio of C18:0 and C18:1 increased. Thereby,the double bond index of fatty acid was reduced. After SA application,both total contents and unsaturation degree of fatty acid could be restored. Low temperature could induce the  expression of CsFAD genes. After SA application,the induction folds of CsFAB2.1CsFAB2.2CsFAD2.1 and CsFAD7 were significantly increased. In summary,in the cucumber seedlings exposed to low temperature,exogenous application of SA could induce the expression of CsFAD genes to increase the unsaturation degree of fatty acids,thereby enhance the stability of cell membranes,and finally increase the stress tolerance of seedlings.

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  • Expression Analysis of WRKY Transcription Factor Isolated from Solanum aculeatissimum Induced by Verticillium dahliae
  • ZHOU Xiao-hui,BAO Sheng-you,LIU Jun,and ZHUANG Yong*
  • Acta Horticulturae Sinica. 2016, 43(10): 1953-1960. DOI:10.16420/j.issn.0513-353x.2016-0310
  • Abstract ( 224 ) HTML ( 484 ) PDF (2614KB) ( 484 )    
  • A total of four unigene sequences that share high homology with WRKY protein were previously obtained from the root transcriptome database of Solanum aculeatissimum.Phylogenetic analysis showed that the four WRKY transcription factors belong to IC,IIa,and III group of WRKY family,respectively.The expression profiles of these four WRKY genes in different organs(root,stem and leaf),as well as in the root of Solanum aculeatissimum of different time after Verticillium dahliae inoculation were analyzed by quantitative real-time PCR.The results showed that the expression levels in root of Unigene6502 and Unigene20920 were significant higher than those in stem and leaf,but the expression levels in leaf of CL1273.Contig2 and CL3641.Contig1 were significantly higher than those in root and stem.The four WRKY genes have different expression patterns upon Verticillium dahliae inoculation.The expression of Unigene6502 and Unigene20920 were up-regulated and then was significantly decreased,while the expression of CL1273.Contig2 and CL3641.Contig1 were up-regulated and down-regulated,respectively.

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  • Genetic Diversity Analysis of Wild Apricot(Prunus armeniaca)Populations in the Ili Valley as Revealed by SRAP Markers
  • LI Ming1,2,HU Xia1,MIAO Xing-jun2,XU Zheng3,and ZHAO Zhong2,*
  • Acta Horticulturae Sinica. 2016, 43(10): 1980-1988. DOI:10.16420/j.issn.0513-353x.2016-0156
  • Abstract ( 296 ) HTML ( 588 ) PDF (2958KB) ( 588 )    
  • The genetic diversity of 14 wild apricot populations which include 212 apricot germplasms in the Ili Valley were detected using SRAP markers. The results showed that 12 primers amplification out 143 bands,122 bands are polymorphic,as a ratio of 85.31%. The wild apricots in the Ili Valley still maintained a relatively high level of diversity(h = 0.2411,I = 0.3708,PPB = 85.31%)and populations Tu with the highest diversity. A relatively high intraspecific genetic diversity(76.42%)and low inter-population genetic differentiation was identi?ed with the moderate gene ?ow value(Nm = 1.3680). The UPGMA and genetic structure analysis both showed that 14 wild apricot populations can be divided into predominantly Huocheng samples in subgroupⅠ,predominantly Gongliu and Yining samples in subgroup Ⅱ and predominantly Xinyuan samples in subgroup Ⅲ,and there is significant correlation  between genetic distance and geographic distance(r = 0.2634,P < 0.05).

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  • Effects of Fermented Crumbs of Branches on the Root Characteristic and Leaf Photosynthesis and Transportation of Young Apple Trees
  • NING Liu-fang,YANG Hong-qiang*,CAO Hui,ZHOU Chun-ran,ZHANG Wei-wei,and MENG Fan-yao
  • Acta Horticulturae Sinica. 2016, 43(10): 1989-1994. DOI:10.16420/j.issn.0513-353x.2016-0273
  • Abstract ( 293 ) HTML ( 587 ) PDF (661KB) ( 587 )    
  • The experiment was carried on using 2-year-old potted apple trees[Malus × domestica Borkh.‘Red Fuji’/Malus hupehensis Rehd.] with the fermented crumbs of apple branches which were applied to the pots with the mass ratio of 2% and 4%. The rates of photosynthesis and transportation,increment of trunk girth,root activity and root architecture parameters from each treatment were investigated periodically. The results showed that the leaf net photosynthetic rate,root activity and increment of trunk girth increased after the fermented crumbs of apple branches were applied to the soil and no significant effects were found on the leaf transportation rate and water use efficiency. The number,surface-area,volume,length,diameter and fractal dimension of new roots increased and the root structure was more complex after the application of 2% fermented crumbs;The number,surface-area,volume and length of new roots decreased after the application of 4% fermented crumbs. The comprehensive analysis showed that the effects of 2% dosage on the growth of root and trunk were better than those of 4%.

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  • Effects of Dazomet Fumigation and Seaweed Biologic Fertilizer on the Malus hupehensis Seedlings and Soil Microbial Quantity Under Replant Conditions
  • LIU Chao,XIANG Li,WANG Sen,CHEN Xue-sen,SHEN Xiang,YIN Cheng-miao*,and MAO Zhi-quan*
  • Acta Horticulturae Sinica. 2016, 43(10): 1995-2003. DOI:10.16420/j.issn.0513-353x.2016-0089
  • Abstract ( 286 ) HTML ( 576 ) PDF (698KB) ( 576 )    
  • To clarify the effects of soil fumigation with dazomet(3,5-dimethyl-1,3,5-thiadiazinane- 2-thione)and seaweed biologic fertilizer on the young apple(Malus hupehensis Rehd.)seedlings biomass and soil microbial quantity under replant conditions. The results showed that compared with the control,dazomet fumigation and seaweed biologic fertilizer all increased the apple seedlings biomass,the effect of the combination was best. Compared with the control,dazomet fumigation,seaweed biologic fertilizer and the combination improved the activities of CAT,POD and SOD in root of the apple seedlings,the effect of the combination was more obvious. Compared with the control,the combination improved total root length,surface area,volume and tips of apple seedlings. Seaweed biologic fertilizer significantly increased the number of bacteria and fungi in the soil than the control,respectively. The combination increased  

    the number of bacteria and the ratio of bacteria and fungi in the soil. The combination of dazoment fumigation and seaweed biologic fertilizer could more effectively reduce the apple replant disease.
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  • Molecular Cloning and Expression Analysis of F3H Gene in Ribes rubrum and R. albrum During Fruit Mature
  • WANG Hai-zhu1,2,YAN Hai-fang2,and XU Qi-jiang1,2,*
  • Acta Horticulturae Sinica. 2016, 43(10): 2003-2011. DOI:10.16420/j.issn.0513-353x.2016-0229
  • Abstract ( 295 ) HTML ( 507 ) PDF (2009KB) ( 507 )    
  • Red currant(Ribes rubrum L.)and white currant(R. albrum L.)fruits were used as materials,two full-length cDNA sequences of F3H gene were cloned using RACE(rapid amplification of cDNA ends),and denoted as RrF3H and RaF3H. Both of them contained a 1 098 bp open reading frame that encoded a deduced protein with 365 amino acid residues. Amino acid sequence alignment showed that the protein had a non-heme dioxygenase domain(DIOX-N superfamily)and 2OG-FeⅡ-dependent dioxygenase superfamily. Phylogenetic analysis showed that,RrF3H and RaF3H had obvious characteristics of species in evolution,which had an relative independent evolutionary branch. Quantitative RT-PCR analysis showed that,during the period of fruit development F3H expression in red currant was much higher than that in white currant. With the deepening of fruit colour,the expression level gradually increased until the period of 75% fruit colour,then the RrF3H decreased rapidly. With the fruit growth of white current,the expression pattern of F3H decreased. And the content of anthocyanins in the same period also showed a decreasing trend. All the results suggested that F3H play a role in the process of fruit coloring.

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  • Effects of Epibrassinolide on the Activities and Gene Expression of Photosynthetic Enzymes in Tomato Seedlings Under Low Light
  • LI Xiang,SANG Qin-qin,SHU Sheng,SUN Jin,and GUO Shi-rong*
  • Acta Horticulturae Sinica. 2016, 43(10): 2012-2020. DOI:10.16420/j.issn.0513-353x.2016-0364
  • Abstract ( 328 ) HTML ( 693 ) PDF (710KB) ( 693 )    
  • In the present work,we investigated the effects of 24-epibrasinolide on growth,activities of photosynthetic enzymes and their gene expressions in low-light sensitive tomato‘Jiersi’leaves under low light stress. The results showed that low light stress significantly reduce the weight,stem diameter and seedling index by 70.5%,13.0% and 77.7% as compared to the control,respectively,but increased plant height by 45.3%. Application of EBR significantly alleviated the low light stress induced-inhibition of growth in seedlings. Under low light,the net photosynthetic rate(Pn)was reduced by 42.8% as compared with the control,while Pn increased by spraying EBR to 72.0% of the control. Low light stress significantly decreased of ribulose-1,5-bisphosphate carboxylase(Rubisco),Rubisco activating enzyme(RCA),fructose-1,6- bisphosphate aldolase(Ald),and fructose-1,6-phosphatase(FBPase)by 41.6%,31.3%,31.4%,and 19.6%,respectively. EBR significantly increased these enzymes activities of photosynthesis under low light stress. Moreover,the mRNA expressions of RbcLRbcSRCAAldFBPaseGAPDHTKSBPase,and PRK were significantly decreased. EBR significantly improve the low light stress-mediated changes of aforementioned mRNA transcripts,which mRNA transcription of SBPase and GAPDH towarded the control values. Transcriptional levels of TK and PRK under low light stress plus EBR were higher than the control ones. These results suggest that EBR could improve photosynthetic capacity through regulating gene expression and activity of key enzymes for carbon assimilation,thus confers tolerance to low light stress on tomato plants.

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  • Cucumber Leaf Senescence Causes in-situ and Vacuolar Degradation of Lipid Droplets
  • GE Shu-jun,ZHAO Dan,ZHOU Xue,SUN Qing,and LIU Lin*
  • Acta Horticulturae Sinica. 2016, 43(10): 2021-2028. DOI:10.16420/j.issn.0513-353x.2016-0266
  • Abstract ( 246 ) HTML ( 736 ) PDF (7531KB) ( 736 )    
  • Leaf senescence induces up-regulated expression of lipid degradation-related genes,and activities of lipases are found to be enhanced in vacuoles. However,it is not clear whether vacuoles are the only site for the degradation of lipid droplets. Therefore,dynamics of lipid droplets in cucumber leaves of different developmental stages were investigated by means of transmission electron microscopy to understand the sites for lipid droplets caused by leaf senescence. Some plastidial lipid droplets in senescent leaves were demonstrated to undergo degradation inside chloroplasts. Degradation began from one side and then expanded to the other. In the cytoplasm some cytosolic lipid droplets were found to be attached to the surface of chloroplasts,indicating that their occurrence might have a close relation to chloroplasts. Some cytosolic lipid droplets revealed a close association with vacuoles,indicating that they were on the pathway to be transported into the vacuoles. Lipid droplets underwent degradation after they were transported into vacuoles. Apparently,cucumber leaf senescence caused in-situ degradation of plastidial lipid droplets and vacuolar degradation of cytosolic lipid droplets. These observations proved that vacuoles are not the only site for the degradation of lipid droplets.

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  • Cloning and Expression Analysis of Catalase CAT1 Gene from Luffa cylindrical
  • WEN Qing-fang*,LIU Jian-ting,ZHU Hai-sheng,CHEN Min-dong,WANG-Bin,and ZHANG Qian-rong
  • Acta Horticulturae Sinica. 2016, 43(10): 2039-2049. DOI:10.16420/j.issn.0513-353x.2016-0406
  • Abstract ( 260 ) HTML ( 487 ) PDF (1704KB) ( 487 )    
  • A length of 1 755 bp cDNA was isolated from Luffa cylindrical by using RACE and RT-PCR techniques,which contained a 1 479 bp open reading frame(ORF)that encoded 492 amino acids,with a predicted molecular weight of 56.98 kD and a hypothetical isoelectric point of 7.126. It shared over 92% identity with the homologous proteins from Cucumis sativusArabidopsis thaliana and Raphanus sativus,proving that it was highly conservative. This gene was named LcCAT1 and the GenBank accession was KP222260. Wolf Psort protection indicated that LcCAT1 protein was located in the peroxisome,and Motif Scan analysis showed that LcCAT1 protein had the characteristic amino acid sequences of catalase proximal heme-ligand and heme active sites in the position of 344–352 and 54–70 sites,respectively,which  suggested that LcCAT1 protein was a typical hydrogen peroxide enzyme(tCAT).The Real time PCR revealed that LcCAT1 exhibited a tissue specific expression,and the expression level in Luffa cylindrical‘Fusi 3leaves was the highest,which was almost five times than that in flowers,fruits,roots and stems. The levels of LcCAT1 were different among six luffa varieties,and the expression in Luffa cylindrical was higher than that in Luffa acutangula Roxb. What is more,the level of LcCAT1 in‘Fusi 3was up-regulated during fresh-cut and post-harvest storage browning processes,which was consistent with the changes observed in peroxidase activity,suggesting that LcCAT1 gene may play a regulation role in luffa browning process.

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  • Cloning and Expression Analysis of Starch Branching Enzyme Gene in Taro(Colocasia esculenta)
  • GU Hui1,2,CHEN Sai-nan1,LI Liang-jun1,*,and CHENG Li-bao1
  • Acta Horticulturae Sinica. 2016, 43(10): 2049-2058. DOI:10.16420/j.issn.0513-353x.2016-0307
  • Abstract ( 238 ) HTML ( 769 ) PDF (1623KB) ( 769 )    
  • In this study,a soluble starch branching gene(SBE)was isolated from the leaf of ‘Xiangtangyu’,a species of taro based on RT-PCR and PCR-RACE methods. The full-length of SBE cDNA(GenBank accession number KX013544)was 3 051 bp in nucleotide containing an open reading frame of 2 538 bp which encoding 845 amino acids. Phylogenetic analysis showed that SBE had more than 85% homolog with maize,potato and rice,respectively. SBE protein contained N-terminal end,C-terminal end and alpha amylase catalytic domain. DNA sequence of SBE was cloned by RT-PCR method,and the full length of DNA was 12 362 bp with 20 exons and 19 introns. During corm formation the expression of SBE in leaf of son-taro plants was significantly higher than that of mother-taro plants,leafstalks,roots,mother-taro and so on,the expression was improved in son-taros,and decreased in grade-taros with development of corm. Totally,we believed that the expression of SBE was synchronous with amylopectin synthesis in son-taros and grade-taros.

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  • A New Chinese Jujube Cultivar‘Shuguang 7’with High Resistance to Fruit Cracking
  • GAO Yun-ru1,2,WANG Zhen-liang1,2,*,SHAO Xue-hong2,LIU Man-guang2,LI Meng-chai2,HOU Jun-ming3,YANG Zhen-jiang4,HAN Hui-zhi3,LI Kai-sen5,and JI Qing-ju3
  • Acta Horticulturae Sinica. 2016, 43(10): 2059-2060. DOI:10.16420/j.issn.0513-353x.2016-0258
  • Abstract ( 247 ) HTML ( 568 ) PDF (2494KB) ( 568 )    
  • ‘Shuguang 7’,a new Chinese jujube(Ziziphus jujuba Mill.)cultivar with high quality and high resistance to fruit cracking was selected from‘Jinsi Xiaozao’in Cangzhou,Hebei. The fruit is oblong with average fruit weight of 7.25 g. The soluble solids content is 26.08% and the fruits taste sour-sweet.‘Shuguang 7’has high resistance to fruit cracking and high stable yield. The fruits ripe in early October in Hebei Province. It could be used dehydration. It is suitable to be cultivated in jujube cultivating area of Hebei Province.

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  • A New Bitter Gourd Cultivar‘Gan Kugua 7’
  • WAN Xin-jian*,MIAO Nan-sheng,ZHANG Jing-yun,and GUAN Feng
  • Acta Horticulturae Sinica. 2016, 43(10): 2061-2062. DOI:10.16420/j.issn.0513-353x.2016-0119
  • Abstract ( 168 ) HTML ( 595 ) PDF (1042KB) ( 595 )    
  • ‘Gan Kugua 7’is a new bitter gourd F1 hybrid developed by crossing high generation inbred line BK0701-8(♀)with high generation inbred line BK0604-1(♂). It’s an medium maturing variety. The first nodal position of female flower on main vine is 18–22. The fruit is stick and regular in shape,with 25–30 cm in length,8 cm in diameter,1.2 cm of flesh thickness. The single fruit weight is around 450 g. It is very good exterior commodity with green-white skin,lustre,and full tumour. It has excellent quality and flavor,with yield of 45 t ? hm-2.
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  • A New Crisp Melon Cultivar‘Huaisu 3’
  • YUAN Ling-yun1,SU Ya2,ZHU Shi-dong1,LI Fang-xia2,ZOU Ming-qian1,JIA Quan-miao1,and WANG Cheng-gang1,*
  • Acta Horticulturae Sinica. 2016, 43(10): 2063-2064. DOI:10.16420/j.issn.0513-353x.2016-0082
  • Abstract ( 255 ) HTML ( 537 ) PDF (1237KB) ( 537 )    
  • ‘Huaisu 3is a new crisp melon cultivar derived from female parent‘S06-1× male line‘W07-2. The cultivar shows better growth potential. The fruit is horn shaped. The skin color is dark green,and the flesh is orange. The flesh thickness is about 2.5 cm,which is crisp. The sugar content is 15.3% in the core and 12.0% in the border. The average fruit mass is about 1.51 kg. It is suitable to be cultivated in the area along the Huaihe River.

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  • A New Lily Cultivar‘Dandie’
  • YUAN Su-xia,LIU Chun,and MING Jun*
  • Acta Horticulturae Sinica. 2016, 43(10): 2065-2066. DOI:10.16420/j.issn.0513-353x.2016-0068
  • Abstract ( 294 ) HTML ( 582 ) PDF (1169KB) ( 582 )    
  • ‘Dandie’is a new lily cultivar of Asiatic hybrid. Its flower is orange-red. This cultivar has short growth period with about 80 days,long florescence with about 20 days,and strong comprehensive resistance. The height of plants is 60–70 cm,and bud number per plant growing from a bulb with a circumference of 14–16 cm is 3–5. In addition,the bulb of this cultivar is edible. It tastes sweet,and it not only has high nutrition value,but also contains a high content of bioactive substances.

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  • A New Rhododendron Cultivar‘Zijin Fenyu’
  • LIU Xiao-qing,SU Jia-le*,LI Chang,HE Li-si,XIAO Zheng,and JIA Xin-ping
  • Acta Horticulturae Sinica. 2016, 43(10): 2067-2068. DOI:10.16420/j.issn.0513-353x.2016-0270
  • Abstract ( 411 ) HTML ( 533 ) PDF (1282KB) ( 533 )    
  • ‘Zijin Fenyu’is a Rhododendron cultivar derived from the cross of female parent‘Yu Qilin’and male parent‘Huohua’. Its flower color is pale pink. The diameter of flower head is about 9.5–10.5 cm. Its petal has five deep lobes. The stamens of some flowers become petals. Upper lobes have striking deep pink spots and its flower heart is green. It is characterized by compact plant type,vigour growth,high temperature and high humidity tolerance. It is suitable for greenhouse pot for the middle and lower reaches of the Yangtze River. Its natural florescence is from early to mid February. Its flowering can last 20–25 days.

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