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2019, Vol.46, No.12 Previous Issue    Next Issue

Research Papers

  • Ethylene Response Factor MdERF3 Promotes Anthocyanin and Proanthocyanidin Accumulation in Apple
  • BI Siqi1,AN Jianping1,WANG Xiaofei1,HAO Yujin1,RUI Lin1,LI Tong1,HAN Yuepeng2,*,and YOU Chunxiang1,*
  • Acta Horticulturae Sinica. 2019, 46(12): 2277-2285. DOI:10.16420/j.issn.0513-353x.2019-0122
  • Abstract ( 374 ) HTML ( 515 ) PDF (1340KB) ( 515 )    
  • Apple ethylene response factor MdERF3 plays an important role in regulating plant growth and fruit ripening. MdERF3 gene was cloned using the‘Gala’apple(Malus × domestica‘Royal Gala’). The pGBKT7-MdERF3 and PET32a-MdERF3 vectors were constructed. And the transcriptional activation activity of MdERF3 protein and its specific binding ability to the GCC(GCCGCC)and DRE(CCGAC)motifs were analyzed by yeast transformation and electrophoretic mobility shift assays. The yeast transformation test showed that MdERF3 had transcriptional activation activity. The electrophoretic mobility shift assay indicated that MdERF3-HIS fusion proteins directly bound to the GCC and DRE motifs. The pCAMBIA1300-MdERF3 overexpression vector was constructed,and genetically transformed into apple calli and Arabidopsis plants,and transiently transformed into apple leaves. The differences in the anthocyanin and proanthocyanidin accumulation were compared between wild-type and transgenic apple lines. The MdERF3-overexpressing apple calli and Arabidopsis seedlings exhibited increased anthocyanin content compared with wild-type controls and overexpression of MdERF3 promoted the accumulation of proanthocyanidin in apple calli and apple leaves. These results suggest that MdERF3 possesses transcriptional activation activity and binds to GCC and DRE motif and MdERF3 gene plays an important role in regulating anthocyanin and proanthocyanidin accumulation.
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  • Differences in Sucrose and Malic Acid Accumulation and The Related Gene Expression in‘Kurakato Wase’Peach and Its Early-ripening Mutant
  • KOU Dandan,ZHANG Ye,WANG Pengfei,Li Dongdong,ZHANG Xueying*,and CHEN Haijiang*
  • Acta Horticulturae Sinica. 2019, 46(12): 2286-2298. DOI:10.16420/j.issn.0513-353x.2019-0187
  • Abstract ( 263 ) HTML ( 442 ) PDF (1804KB) ( 442 )    
  • In order to reveal the mechanism of differences in soluble sugar and organic acid accumulation in fruit development between‘Kurakato Wase’peach and its early-ripening mutant,the difference content of soluble sugar and organic acid involving in the development of peach fruit were studied in‘Kurakato Wase’peach and its early-ripening mutant. During fruit development,the expression profiles of sucrose and malic acid metabolism-related genes were analyzed. The expression of relevant genes with obvious trends were validated by qRT-PCR. The result showed that the content changes of glucose, fructose,sucrose,sorbitol,citric acid,malic acid and shikimic acid of both were basically consistent during fruit development,and the contents of malic acid and soluble sugars had no significant differences when ripened between‘Kurakato Wase’peach and its early-ripening mutant. The contents of sucrose were higher than those of‘Kurakato Wase’peach,and the content of malic acid were lower than those of‘Kurakato Wase’peach in 59 and 71 days after flowering. RNA-seq data and correlation analysis showed that the differences of sucrose content were mainly affected by PpSUS1,PpSPS1,PpINV2 and PpTMT1-1,the expression levels of PpSUS1 and PpTMT1-1 increased gradually during fruit development,which is consistent with the change trend of sucrose content between‘Kurakato Wase’peach and its early-ripening mutant. The expression of PpSPS1 is positive correlation with sucrose. The difference of malic acid content was affected by PpMDH1-2 and PpNADP-ME1. The expression of PpMDH1-2 reduced gradually during fruit development. The expression of PpMDH1-2 was notably lower than‘Kurakato Wase’peach in 59 and 71 days after flowering. PpPEPCK1,PpPEPCK2 paly auxiliary roles for the content accumulation of malic acid.
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  • Effect of 1-Methylcyclopropene(1-MCP)on Quality and Chlorophyll Maintenance of Postharvest‘Yuluxiang’Pear
  • MA Fengli,DU Yanmin,WANG Yang,TONG Wei,LIU Bailin,WANG Wenhui*,and JIA Xiaohui*
  • Acta Horticulturae Sinica. 2019, 46(12): 2299-2308. DOI:10.16420/j.issn.0513-353x.2018-0676
  • Abstract ( 261 ) HTML ( 409 ) PDF (867KB) ( 409 )    
  • Effect of 1-methylcyclopropene(1-MCP)on chlorophyll degradation and quality maintenance of postharvest of‘Yuluxiang’pear fruits were investigated,providing a theoretical basis for the‘Yuluxiang’pear about preservation technology. In this study,two groups of ‘Yuluxiang’pear were used as experimental materials,one group was treated with 1.0 μL ? L-1 1-MCP at 20 ℃,while the other group was used as control and kept at 20 ℃ without 1-MCP treatment. Chlorophyll content and fruit firmness,soluble solids,titratable acid,vitamin C content,respiration rate and ethylene production were measured. Moreover,the expression level of related genes to chlorophyll degradation were detected by real-time quantitative PCR. The results showed that the firmness and soluble solids content of ‘Yuluxiang’pear were unsignificantly affected between 1-MCP treatment and the control. But 1-MCP could significantly inhibited the decrease of titratable acid content and vitamin C content,the expression of PbETR1 and PbETR2,and delayed the respiration rate and ethylene retention time;1-MCP treatment could inhibit the expression of PbCLH1 gene,delay the downstream reaction in the pathway of chlorophyll catabolism,so as to delay the degradation of chlorophyll,and maintain the quality and chlorophyll of‘Yuluxiang’pear fruit during storage.
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  • Stigma Shape Development and Receptivity of‘Zhongqiu Sucui’Chinese Jujube
  • SHAO Fengxia,WANG Sen*,CHEN Jianhua,CHEN Juan,HONG Rongyan,TANG Yan,and WANG Jia
  • Acta Horticulturae Sinica. 2019, 46(12): 2309-2322. DOI:10.16420/j.issn.0513-353x.2019-0185
  • Abstract ( 296 ) HTML ( 322 ) PDF (4899KB) ( 322 )    
  • In order to improve the efficiency of cross breeding in Chinese jujube,the stigma morphology and receptivity at different development stages were observed by microscopy,and the pollen germination on stigma was observed regularly to detect the receptivity of stigma at different development stages. The results showed that the stigma of Ziziphus jujuba‘Zhongqiu Sucui’was dry stigma and bifid, and there was no mucus secretion on the surface. The mature stigma was covered with a layer of nearly circular papillary cells. The morphological characteristics of the Z. jujuba‘Zhongqiu Sucui’stigma exhibited a specific pattern as the opening of a single flower progressed. From the bud oblate stage to the early flowering stage,two styles were very close to one another,together as a cone shape. The space between the two styles began to increase from the sepal flattening stage and the styles started to grow towards two sides. At the petal flattening stage,the angle between the two styles exhibited a“V”shape,At the stamen flattening stage,the angle between the two styles exhibited a“Υ”shape,which lasted until the ovary enlargement stage. The bottom width and length of the style increased as the flowering process progressed. The stigma was flat at the bud oblate stage;from the bud fat stage to the petal flattening stage,the stigma had a pointed,round morphology. Its submicroscopic morphology showed that at that time,the stigmatic surface changed from being wrinkled to full-bodied,then to bulging outwards. At the stamen flattening and petal wilting stages,the stigma was curled outwards,bulging upwards,with a relatively large surface area. At the stamen wilting and ovary enlargement stages,the stigma shrank and dried up. From the floral bud oblate stage to the petal flattening stage,the colour of the Z. jujuba‘Zhongqiu Sucui’stigma was green,and then it began to lose its green colour. At the stamen flattening stage,the colour of the stigma was light yellowish-brown. At the petal wilting stage,the colour of the stigma was yellowish-brown,and it turned black at the stamen wilting and ovary enlargement stages. From the floral bud oblate stage to the stamen flattening stage,the colour of the stigma remained green,and therefore the slight differences in the green colour during each stage cannot be used to determine the level of stigma receptivity. However,it could be sure when the stigma began to lose its green colour,the stigma receptivity decreased. From the floral bud flattening stage to the ovary enlargement stage,the stigmatic papilla cells on the stigmatic surface at different flowering stages exhibited a changing pattern,first developing to maturity,then reaching the most active stage,followed by shrinking and becoming shrivelled,senesced,and degraded. As the stigmatic papilla cells developed towards maturity and reached physiological senescence,the stigma receptivity exhibited a changing pattern of“no receptivity-weak- strong-weak-no receptivity”. Stigma receptivity can be determined based on the developmental level of the stigmatic papillae on the stigmatic surface of the jujube flowers. The optimal stigma receptivity period of Z. jujuba‘Zhongqiu Sucui’lasted from the sepal flattening stage to the petal flattening stage,which lasted approximately 6–8 h.
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  • Cloning and Functional Analysis of Male Sterile 2-like Protein Gene(MSLP)in Citrus
  • QIU Wenming1,ZHANG Zuming2,XU Yuhai1,HE Xiujuan1,SUN Zhonghai1,TONG Zhu1,XIAO Cui1,WU Liming1,and GUO Wenwu2,*
  • Acta Horticulturae Sinica. 2019, 46(12): 2323-2334. DOI:10.16420/j.issn.0513-353x.2019-0126
  • Abstract ( 246 ) HTML ( 398 ) PDF (1848KB) ( 398 )    
  • To elucidate the functions of male sterile 2-like protein(MSLP)in citrus,the full-length cDNAs of MSLP were cloned in six typical citrus varieties using RT-PCR. Four transcripts of MSLP were identified,and only MSLP-B existed in all varieties. Structure analysis showed MSLP contained three conserved fatty acyl-CoA reductase(FAR)C terminal domains. Phylogenetic analysis suggested that MSLP-B clustered with sweet orange FAR-like protein and clementine FAR3 protein,and they had closer genetic relationship with FAR-proteins in rosaceous plant like peach,rose,sweet cherry and plum. Southern blotting showed there were 5–8 copies of MSLP in citrus. Transient expression of MSLP-B-GFP in Nicotiana benthamiana leaves indicated it functioned in cytoplasm. qRT-PCR results showed the expression of MSLP-B in floral organs of seedless Ponkan was up-regulated compared with common seedy Ponkan. Over-expression of MSLP-B in tobacco showed that the ratio of stylar length to filament length in mature flower was higher than non-transgenic tobacco. The viability of pollen grains was normal in transgenic lines. However,the number of unsaturated elliptic pollen grains increased significantly.
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  • Analysis of Differentially Expressed Genes of Exogenous GA3 Releasing Aerial Bulbs Dormancy in Garlic Based on Transcriptome Sequencing
  • DONG Yuhui1,WANG Lixia1,GU Qiyu1,LIU Zhongliang2,SUN Xiudong1,and LIU Shiqi1,*
  • Acta Horticulturae Sinica. 2019, 46(12): 2335-2346. DOI:10.16420/j.issn.0513-353x.2019-0226
  • Abstract ( 202 ) HTML ( 405 ) PDF (1188KB) ( 405 )    
  • Exogenous GA3 was used to break the dormancy of Jinxiang aerial bulbs. High throughput sequencing technology was used to sequence the transcriptome of dormant and GA3-treated aerial bulbs. To analyze and screen differentially expressed genes in response to exogenous GA3,and to explore the relationship between differentially expressed genes and the release of aerial bulb dormancy by GA3. The results showed that exogenous GA3 could effectively break the dormancy of aerial bulbs;4 588 up-regulated genes and 55 857 down-regulated genes were obtained by transcriptome sequencing;28 107 differentially expressed genes were annotated in KEGG;among which the most differentially expressed genes were annotated in metabolic pathway;followed by genetic information processing pathway. More differentially expressed genes were enriched in plant hormone signal transduction pathway and the enrichment results were significant. Significant differentially expressed genes GAI,SLR1,PP2C involved in GA,ABA and ZR signal transduction were screened out. Their expressiones were consistent with the change of endogenous hormone content in aerial bulbs. It was speculated that exogenous GA3 could activate the biosynthesis and signal transduction of gibberellin by inhibiting the expression of DELLA gene,a negative regulator of GA signal pathway,so as to release dormancy of aerial bulbs. At the same time,the significant decrease of PYL expression resulted in a relative decrease of abscisic acid content,which was conducive to the relief of dormancy. The results of qRT-PCR showed that the sequencing results were consistent with the actual results,which indicated that the data of transcriptome sequencing were true and reliable.
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  • Construction of Melon Genetic Linkage Map and QTL Analysis of Fruit Related Traits in Snake Melon × Wild Type Melon Genetic Background
  • WANG Chenhui,LUAN Feishi*,GAO Peng*,LIU Shi,and XIE Zhiqiang
  • Acta Horticulturae Sinica. 2019, 46(12): 2347-2358. DOI:10.16420/j.issn.0513-353x.2019-0118
  • Abstract ( 200 ) HTML ( 303 ) PDF (2838KB) ( 303 )    
  • Based on the importance on the fruits related traits on melon production,QTLs locus linked with the fruits related traits in melon was analyzed in this paper. F2 populations obtained from Snake melon(Cucumis melo L.)‘M4-75’and wild type melon(Cucumis melo L.)‘X207’as parents were used as materials. Cleaved amplified polymorphic sequence(CAPS)markers were developed to construct a genetic linkage map and detect the QTLs(quantitative trait locus)related with fruit traits in melon based on the parental genome re-sequencing data. As the results,totally 180 CAPS markers were screened which showed polymorphism between parents and for the genetic linkage map construction. Finally,the map contained 165 markers and grouped into 12 linkage groups corresponded to the 12 chromosomes in melon,spanning a total length of 1 019.81 cM,with an average of 6.18 cM between markers. Meanwhile,fruits related traits(fruit shape,flesh thickness and flesh pH)in melon were all quantitative traits. Totally 14 QTLs associated with the traits were detected which distributed among Chromosome 1,2,3,4,5,6,7,10,11. The LOD(log of odds)score ranged from 2.67 to 9.63,which could explain the phenotypic variation rate of 7.60%–35.18%. Nine QTLs related with fruit shape,3 QTLs for flesh thickness and 2 QTLs for flesh pH.
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  • Effects of Regeneration and Abiotic Stress Tolerance in Polyploid Lilium davidii var. unicolor and Lilium pumilum in Vitro
  • WANG Chunxia,YIN Yue,WANG Zhiping,YAN Rui,Fu Linlan,and SUN Hongmei*
  • Acta Horticulturae Sinica. 2019, 46(12): 2359-2368. DOI:10.16420/j.issn.0513-353x.2019-0190
  • Abstract ( 199 ) HTML ( 397 ) PDF (5551KB) ( 397 )    
  • In order to clarify the growth characteristics of the new polyploid germplasm in Lilium,variation of plant regeneration ability,drought resistance and saline-alkali resistant ability were compared between the diploid seedlings and its homologous polyploid seedlings in vitro in Lilium davidii var. unicolor and Lilium pumilum DC. Fisch. The results showed that the duration for the formation of adventitious buds and somatic embryos in the tetraploid plants scales were significantly shortened and the regeneration seedlings were larger with the stable ploidy compared with the diploid plant scales. The regeneration ability of the aneuploid scales was reduced significantly,and the callus without embryo ability was produced. The regenerated plants produced by the mixoploid scales have different morphology and unstable traits,and the induced somatic embryos gradually brown during culture. PEG-6000 and NaHCO3 were used to simulate drought stress and saline-alkali stress,respectively. Under low concentration stress,the tetraploid seedlings kept good adaptability,while the diploid seedlings were more sensitive. The tetraploid plants of both lily species maintain their stability under stress by extending roots length and keeping a good water balance in leaves.
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  • Identification and Expression Profiling Analysis of TCP Family Genes Involved in Growth and Development in Camellia sinensis
  • WEN Beibei1,LUO Yong1,LIU Dongmin1,ZHANG Xiangna1,LI Juan1,2,3,WANG Yingzi1,2,3,WANG Kunbo1,2,3,*,and HUANG Jian’an1,2,3,*
  • Acta Horticulturae Sinica. 2019, 46(12): 2369-2382. DOI:10.16420/j.issn.0513-353x.2019-0320
  • Abstract ( 199 ) HTML ( 412 ) PDF (4145KB) ( 412 )    
  • In this study,a genome-wide analysis of TCP genes was performed in Camellia sinensis,including identification,chromosomal localization,gene structure,phylogenetic analyses and expression analysis in different organs. In all,thirty-six proteins were identified with typical TCP domains. The length of CDS is from 453 to 1 824 bp,and proteins’ length is from 150 to 607 aa(amino acids).These identified CsTCP members were named as CsTCP1–CsTCP30. Phylogenetic and comprehensive bioinformatics analyses of these genes revealed that Camellia sinensis TCP family proteins could be classified into two classes(ClassⅠand ClassⅡ),which could be further divided into eight subgroups(A–H). Among them,ClassⅠ are also known as PCF or TCP-P,and ClassⅡcan be clustered into CYC/TB1 and CIN types. Gene structures and conserved motif analysis of CsTCPs indicated that different types of TCP genes had undergone different evolutionary processes and the genes in the same clade had similar chromosomal location,gene structure,and conserved domains. Except for CsTCP21b the expression of all 35 CsTCP genes could be detected in tea leaves of different growth stages,and qRT-PCR experiments results revealed that the CsTCPs gene family exhibited distinct expression pattern in different tissues. GA3 and ABA treatments could promote or repress the expression of CsTCPs,significantly.
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Research Notes

  • Effects of Tagetes erecta Biofumigation on Apple Replanted Soil Environment and Physiological Characteristics of Malus hupehensis Seedlings
  • WANG Xiaofang1,2,*,XIANG Kun2,*,WANG Yanfang3,LI Qianjin4,JIANG Weitao1,SHENG Yuefan1,WANG Haiyan1,CHEN Xuesen1,YIN Chengmiao1,and MAO Zhiquan1,**
  • Acta Horticulturae Sinica. 2019, 46(12): 2383-2396. DOI:10.16420/j.issn.0513-353x.2018-0851
  • Abstract ( 154 ) HTML ( 343 ) PDF (886KB) ( 343 )    
  • To explore the feasibility of preventing and controlling apple replant disease by biofumigation using Tagetes erecta power,and to study its effects on soil environment and physiological characteristics of Malus hupehensis Rehd. seedlings. A pot experiment was carried out,and the seedlings of Malus hupehensis Rehd. were planted in pots with old apple orchard soil. The experiment included 5 treatments,that was,the control(Replanted soil),0(Replanted soil + film mulching),0.05 %[Replanted soil + 0.05%(w/w)Tagetes erecta powder + film mulching],0.2%(Replanted soil + 0.2% Tagetes erecta powder + film mulching),0.5%(Replanted soil + 0.5% Tagetes erecta powder + film mulching). And the soil nutrient content,soil phenolic acid content,gene copies of Fusarium oxysporum,root respiration rate and antioxidant enzyme activity,leaf photosynthetic parameters and chlorophyll fluorescence parameters were measured. The results showed that treatment with 0.2% Tagetes erecta power could significantly increase soil nutrient content,reduce soil phenolic acids content,decrease gene copies of Fusarium oxysporum,increase root respiration rate and antioxidant enzyme activity,reduce MDA content;and could effectively alleviate the decrease of photosynthetic and fluorescence parameters caused by the replant disease,such as photosynthetic rate(Pn),the maximum photochemical efficiency of PSII(φPo),the actual photochemical efficiency(ΦPSII),the photochemical quenching(qP)and the number of reactive centers per unit area(RC/CSm),and change the OJIP curve shape. All the indicators of 0.05% and 0.5% were improved compared with the control,but the effect was not as good as that of 0.2% concentration. So the suitable concentration of Tagetes erecta biofumigation(0.2%)could improve soil environment,promote root growth,enhance the photosynthetic capacity and improve the energy distribution of M. hupehensis seedling leaves,and then alleviate the damage of PSII.
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  • Detection and Diversity Analysis of Apple chlorotic leaf spot virus in Northeast China
  • CHEN Yahan1,2,SUN Pingping1,MA Qiang1,*,ZHAO Mingmin1,WU Zhanmin3,and LI Zhengnan1,*
  • Acta Horticulturae Sinica. 2019, 46(12): 2397-2405. DOI:10.16420/j.issn.0513-353x.2018-1033
  • Abstract ( 142 ) HTML ( 314 ) PDF (5260KB) ( 314 )    
  • In order to clarify the incidence and diversity of Apple chlorotic leaf spot virus(ACLSV)in northeast China and Inner Mongolia,a total of 165 apple leaf samples were collected from 8 sampling points in these areas and tested by reverse transcription-polymerase chain reaction. The average incidence of ACLSV among the collected samples was 60.6%,suggesting its wide spread in the surveyed regions. The fragments of the complete coat protein(CP)genes generated from 25 samples were cloned and sequenced. Pairwise comparison analysis showed that the nucleotide and amino acid sequence identities of CP genes of the 25 isolates ranged from 84.0%–99.7% and 90.7%–99.5%,respectively,while they shared the nucleotide and amino acid sequence identities with another 13 ACLSV isolates of 67.7%–99.7% and 71.0%–99.5%,respectively. The ACLSV isolates worldwide were grouped into four groups(B6,P205,SHZ and Ta Tao 5),and the 25 ACLSV isolates were located in the groups B6,P205 and SHZ. No significant evidence of recombination was found among the 25 complete CP sequences.
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  • Screening and Identification of Bacillus Antagonist Against Anthracnose of Jujube and Analysis of Its Antifungal Substances
  • LI Jiawei,XU Lanyi,WANG Dongshuang,Lü Lijie,CHEN Xiaomeng,and ZHANG Dongdong*
  • Acta Horticulturae Sinica. 2019, 46(12): 2406-2414. DOI:10.16420/j.issn.0513-353x.2019-0052
  • Abstract ( 152 ) HTML ( 264 ) PDF (1797KB) ( 264 )    
  • Bacillus strains isolated from jujube fruits with significant antagonistic activity against anthracnose was screened by confrontation culture method. The control effect of antagonistic bacteria against anthracnose was tested by in vivo experiment using winter jujube fruits. The activity and structure of the antifungal substance from the fermentation supernatant of the antagonistic bacterium were tested by inhibition zone method and liquid chromatography-mass spectrometry(LC–MS),respectively. Strain Tj-19 which exhibited significant antagonistic activity against jujube anthracnose was identified as Bacillus amyloliquefaciens based on the morphological observation,physiological and biochemical characteristics and 16S rDNA and gyrB sequence analysis. The biocontrol effect of Tj-19 bacteria cell and its fermentation supernatant on anthracnose of winter jujube fruit reached 74.9% and 75.8%, respectively. The main antifungal substances from the fermentation broth of strain Tj-19 were Iturin A and Iturin B.
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  • Genetic Analysis and Measurement Parameter Comparison of the Purple Trait in Purple Heading Chinese Cabbage
  • XUE Yihua,HE Qiong,CHEN Lin,LI Ru,and ZHANG Lugang*
  • Acta Horticulturae Sinica. 2019, 46(12): 2423-2428. DOI:10.16420/j.issn.0513-353x.2019-0160
  • Abstract ( 316 ) HTML ( 272 ) PDF (1424KB) ( 272 )    
  • Purple heading Chinese cabbage inbred line‘14S838’and common Chinese cabbage inbred line‘15S1080’were used as male parent and female parent,respectively,and F1 and F2 populations were constructed to study the genetic law of purple heading trait in Chinese cabbage. The results showed that the inner leaves of leaf-head were purple in F1 population,but the purple area was smaller than that of its purple parent‘14S838’. The segregation ratio of purple heading individuals to non-purple individuals fitted to the ratio of 3︰1 in F2 population,which indicates that purple heading of Chinese cabbage was a quality trait and consistent with genetic pattern of a single dominant gene. Purple height ratio(purple height/leaf head height),purple width ratio(purple width/leaf head width)and purple area ratio(purple area/leaf head area)were used to describe purple size trait in purple single plant of the F2 generation,and results indicate that the broad-sense heritability of these three parameters was 94.00%,87.72% and 83.33%, respectively. The result meant that the variation of purple size was mainly controlled by genetic factors,while the external environment showed little influence. The frequency distribution histograms showed that the purple height ratio and the purple width ratio were accorded with normal distribution but the purple area ratio showed positively skew distribution,which indicated that the purple size trait was a quantitative character. Further analysis found the high correlation existed between purple height ratio,purple width ratio and purple area ratio. According to the measurement operation,the purple area ratio was accurate but more complicated to measure. However,measurement of the purple height ratio was simple. Moreover,the purple height ratio was accompanied with high hereditary capacity and highly correlated with the purple area ratio. Hence,the purple height ratio can be used instead of the purple area ratio to define the purple size of the purple heading Chinese cabbage.
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  • Genetic Mapping and Candidate Prediction of the sa1 Gene Controlling Anther Development in Diploid Potatoes
  • WANG Pei1,TANG Die2,LI Yumei3,YANG Zhongmin4,ZHU Guangtao1,HUANG Sanwen2,and ZHANG Chunzhi2,*
  • Acta Horticulturae Sinica. 2019, 46(12): 2429-2436. DOI:10.16420/j.issn.0513-353x.2019-0407
  • Abstract ( 194 ) HTML ( 333 ) PDF (1552KB) ( 333 )    
  • In a previous study,we identified a short anther mutant sa1,which is a potential material for developing male sterile lines. In this study,we constructed a BC1F2 segregating population of sa1. By bulked segregant analysis(BSA-seq),we found this trait was controlled by a single gene which located in a 0–33.9 Mb interval on chromosome No. 6. To further map the sa1 gene,we used more BC1F2 progeny to screen the recombinants. Based on the genotype and phenotype of recombinants,the sa1 gene was narrowed to a 1.4 Mb interval. According to the annotation information of the potato reference genome,this region contains 40 genes. In addition,we predicted the candidate genes using the transcriptome data from normal and short anthers.
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  • Studies on Heat Tolerance of Different Summer Chrysanthemum Cultivars
  • KONG Lingjie,CHEN Yanbo,and WANG Yaqin*
  • Acta Horticulturae Sinica. 2019, 46(12): 2437-2448. DOI:10.16420/j.issn.0513-353x.2019-0145
  • Abstract ( 394 ) HTML ( 375 ) PDF (4541KB) ( 375 )    
  • In order to select the most suitable cultivar for planting in the tropical area of southern China,the tolerance toward high temperature stress of six summer chrysanthemum cultivars were studied. The summer chrysanthemum cultivars introduced from Nanjing,including‘Fengyunli’,‘Zaohuang’,‘Sijiju’,‘Aoyunjinkui’(referred to as‘Aojin’),‘Aoyuncaidie’(referred to as‘Aocai’),‘Aoyunhongxing’(referred to as‘Aohong’),and the Guangdong local chrysanthemum were used to be treated under high temperature stress at seedling and flowering stages,respectively. The results showed that the seedlings of‘Aojin’had no obvious heat injury under high temperature stress. Compared with those of the Guangdong local chrysanthemum,the cells were more stable in the heat-treated seedlings of ‘Aojin’,supported by the evidence that the increased extents of malondialdehyde(MDA)content,conductivity and chlorophyll degradation were smaller in‘Aojin’. Moreover,the proline content in the ‘Aojin’seedlings was significantly increased,and the survival rate of‘Aojin’seedlings was highest after recovery. In addition,the expression levels of heat shock genes CmHSP70,CmHSP90,CmHSP and CmsHSP in the‘Aojin’seedlings were increased significantly and reached the highest point after 1 h of high temperature treatment. The degrees of wilting and the physiological indexes of the‘Aojin’flowers under high temperature stress were consistent with those from the seedling treatment. According to the performance of these cultivars in both seedling and flowering stages,the heat resistance of‘Fengyunli’was inferior to‘Aojin’,while the heat resistance of‘Zaohuang’or‘Sijiju’was similar to that of Guangdong local chrysanthemum,which was inferior to‘Fengyunli’,and the heat resistance of‘Aohong’and‘Aocai’was worst. Collectively,our study indicated that‘Aojin’is the best heat-resistant cultivar and is suitable for planting in the tropical area of southern China.
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New Technologies and New Methods

  • Comparison of Methods of Genomic DNA Extraction from Paphiopedilum armeniacum
  • SHAO Yalin1,SI Junbo1,CHANG Wei2,?,SHI Jialian2,ZHOU Xiang1,and DING Yong1,?
  • Acta Horticulturae Sinica. 2019, 46(12): 2449-2454. DOI:10.16420/j.issn.0513-353x.2019-0054
  • Abstract ( 160 ) HTML ( 327 ) PDF (876KB) ( 327 )    
  • This research aimed to isolate high quality genomic DNA from the mature leaves of Paphiopedilum armeniacum using CTAB method,improved CTAB method,high salt low pH method and SDS method. The quality of genomic DNA including integrity and purity was examined through OD value and agarose gel electrophoresis. The results showed that the genomic DNA obtained from the mature leaves of P. armeniacum with CTAB method and high salt and low pH method had poor purity,poor integrity and low yields,and the DNAs were degraded seriously and polluted by proteins. The genomic DNA extracted by the SDS method was intact without degradation,but was polluted by proteins and the DNA purity was low. Fortunately,the genomic DNA obtained by improved CTAB method was intact without degradation,had higher purity and higher yield. The ratio of A260/A280 is 1.7–1.9. It was proved that proteins,polyphenols and polysaccharides rich in the leaves cells of P. armeniacum,could be removed effectively,which had high purity and quality and could be used in molecular biological research,like PCR directly.
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New Cultivars

  • A New Early Ripening Sand Pear Cultivar‘Xiaqing’
  • XIE Zhihua,QI Kaijie,WU Jun,WU Juyou,YIN Hao,TAO Shutian,GU Chao,WU Huaqing,HUANG Xiaosan,ZHANG Huping,WANG Peng,and ZHANG Shaoling*
  • Acta Horticulturae Sinica. 2019, 46(12): 2455-2456. DOI:10.16420/j.issn.0513-353x.2019-0647
  • Abstract ( 171 ) HTML ( 328 ) PDF (890KB) ( 328 )    
  • ‘Xiaqing’is a new early ripening sand pear cultivar selected from the cross‘Niitaka’בXizilü’. The fruit shape is mainly round,the average fruit weight is 330 g,and it has green peel and smooth surface with almost no russet and unconspicuous fruit dots. The fruit core is small,and the flesh is white,crisp,fine-texture,juicy and sweet. The soluble solids content is 11.5%–12.0%,accompany with 0.16% titratable acid. The fruit growth period is 115 d around,and it matures in late July in Nanjing,Jiangsu Province. The yield is up to 11 249 kg ? hm-2 in the fourth year.
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  • A New Early Ripening Navel Orange Cultivar‘Qingqiu’
  • CHEN Shanchun*,LEI Tiangang,HE Yongrui,PENG Aihong,XU Lanzhen,and ZOU Xiuping
  • Acta Horticulturae Sinica. 2019, 46(12): 2457-2458. DOI:10.16420/j.issn.0513-353x.2018-0892
  • Abstract ( 270 ) HTML ( 228 ) PDF (1294KB) ( 228 )    
  • ‘Qingqiu’,a new early ripening navel orange cultivar,was selected from‘Meihong’navel orange. It’s fruits are oval or long oval,with a fruit shape index of 1.18. The average fruit weight is 270 g. The peel is orange red and its surface is smooth. The flesh is crisp,sweet,and rich flavor. Regional trial for‘Qingqiu’was performed at multiple locations in Chongqing. The results showed that its fruits ripen from the mid to late October and that the fruits are of good quality with soluble solids content of 12.9%,total acid content of 0.67%,vitamin C content of 0.59 mg ? mL-1,and edible rate of 70.8%. Filed investigation indicated that‘Qingqiu’has the characteristics of high fruit-setting rate and high yield. It had an average yield of 9.9 kg and 23.4 kg per tree in two years after planting and top grafting,respectively.
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  • A New Tomato Cultivar‘Wanhong 16’
  • WANG Yan1,3,JIANG Haikun1,3,YAN Congsheng1,3,WANG Mingxia1,3,LI Hongying2,JIA Li1,3,ZHANG Qi’an2,3,YU Feifei1,3,and FANG Ling1,3,*
  • Acta Horticulturae Sinica. 2019, 46(12): 2459-2460. DOI:10.16420/j.issn.0513-353x.2019-0701
  • Abstract ( 137 ) HTML ( 280 ) PDF (811KB) ( 280 )    
  • ‘Wanhong 16’is a new early-medium ripening hybrid tomato. It is of infinite-growth-type. The first inflorescence sets on the 7th–8th nodes. The matured fruits are red in color and oblate in shape. The average single fruit weight is 200 g. The fruits has good hardness and long shelf life. The soluble solids content is 4.9%. The yield is about 135 t ? hm-2 in protected fields. It is tolerant to low temperature and low light. It is resistant to leaf mould and TYLCV(Tomato yellow leaf curl virus)in field. It is suitable for protected cultivation in Jianghuai Basin and the same ecological area.
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  • A New Colored Foliar Cultivar of Populus deltoides‘Xuanhong’
  • WANG Aike1,*,CHENG Xiangjun2,FU Zhenzhu3,WANG Hui1,and WANG Ruili1
  • Acta Horticulturae Sinica. 2019, 46(12): 2461-2462. DOI:10.16420/j.issn.0513-353x.2018-0485
  • Abstract ( 209 ) HTML ( 201 ) PDF (194KB) ( 201 )    
  • Populus deltoides‘Xuanhong’is a new colored foliar tree cultivar,which was obtained from a bud mutation of‘Zhonghong’. It is a kind of male cultivar with no floating catkins in spring and containing brilliantly colored leaves. During the growth period,the petioles,leaf veins,young stems and growing shoots of it are always showing red coloration,and then the red leaves change to orange red or orange yellow in defoliating stage gradually. This new poplar cultivar can be reproduced by cutting or grafting. Thus,it is a preferred ornamental cultivar for landscape or garden with simple cultivation.
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