Screening and Identification for Bacterial Wilt Resistance Accession TK083 in Tomato

doi:10.16420/j.issn.0513-353x.2024-0084

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (6): 1477-1487.doi: 10.16420/j.issn.0513-353x.2024-0084

• Genetic & Breeding?Germplasm Resources?Molecular Biology • Previous Articles Next Articles

Screening and Identification for Bacterial Wilt Resistance Accession TK083 in Tomato

FANG Junyi¹^,², WU Weifeng¹, LU Qiao³^,⁴, LING Hongqing³^,⁴, and KONG Danyu¹^,²^,^*()

¹ Lushan Botanical Garden，Jiangxi Province and Chinese Academy of Sciences，Jiujiang，Jiangxi 332800，China
² School of Life Sciences，Nanchang University，Nanchang 330031，China
³ Hainan Seed Industry Laboratory，Sanya，Hainan 572024，China
⁴ Institute of Genetics and Developmental Biology，Chinese Academy of Sciences，Beijing 100101，China

Received:2024-01-14 Revised:2025-03-03 Online:2025-06-20 Published:2025-06-20
Contact: and KONG Danyu

Abstract

Abstract:

In the northern part of Jiangxi Province，130 tomato accessions were naturally screened for bacterial wilt resistance，and the seedling resistance of these accessions to the local dominant Ralstonia solanacearum strain RS100 was verified，and TK083 was found to be an excellent tomato resistant to bacterial wilt accessions. After inoculation with Ralstonia solanacearum，the concentration of the pathogen in the xylem sap of TK083 was much lower than that of susceptible tomato‘Moneymaker’. The activities of SOD and POD in the leaves of TK083 increased markedly，while the MDA content showed a slight increase in the early stages post-inoculation. The activity of chitinase and β-1，3-GA increased in the early and late stages of inoculation respectively，indicating that they were involved in different stages of immune response. RT-qPCR results revealed that the expression levels of SlNAP1，SlERF2a and SlERF3，which are involved in the immune regulation of bacterial wilt，were significantly up-regulated by Ralstonia solanacearum in TK083 roots. These results suggested that TK083 likely employs multiple pathways to coordinately combat the infection of Ralstonia solanacearum.

Key words: tomato, bacterial wilt, Ralstonia solanacearum, accession, resistance, identification

FANG Junyi, WU Weifeng, LU Qiao, LING Hongqing, and KONG Danyu. Screening and Identification for Bacterial Wilt Resistance Accession TK083 in Tomato[J]. Acta Horticulturae Sinica, 2025, 52(6): 1477-1487.

Figures/Tables 7

Fig. 1 The natural incidence of bacterial wilt in 130 tomato accessions under field conditions ClassI：Incidence ≤ 5%；ClassⅡ：5% < incidence ≤ 30%；ClassⅢ：30% < incidence ≤ 75%；ClassⅣ：75% < Incidence. SLL：Solanum lycopersicum var. lycopersicum；SLC：S. lycopersicum var. cerasiforme；SP：S. pimpinellifolium

Table 1 Primers for qRT-PCR

引物名称 Primer name	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer	片段/bp Fragment length	参考文献 Reference
qSlNAP1	CCAAATAGAGCAGCTGTGTCA	TTGGTGGTTTGCCTTTGTAA	121	Wang et al.,2020
qSlERF2a	TATGCACAATTACTTCGCGATG	GCTTGTTGTTGTTGTTGTTG	155	Ke et al.,2023
qSlERF2b	CGACGATACAGAGGAGTTAGAC	AAAAGTACCTAACCAAACACGC	96	Ke et al.,2023
qSlERF3	GGTGCAGTTACAGCTATGGC	CTAGCAGCGTTATCATAAGC	191	Pan et al.,2010
qSlWRKY33	AAGGCAACAACAGACCACTG	CCATCTTCCGCCTTCTGTTC	162	Ke et al.,2023
qSlUBI	TCGTAAGGAGTGCCCTAATGCTGA	CAATCGCCTCCAGCCTTGTTGTAA	119	姜静等,2017

Fig. 2 Evolutionary tree of Ralstonia solanacearum strain RS100 with other Ralstonia representative sequences

Fig. 3 Screening of tomato accession for resistance to Ralstonia solanacearum A：Incidence of seedling inoculation；B：Comparison of bacterial wilt incidence between natural screening in the field and seedling inoculation screening

Fig. 4 Phenotype（A），number of xylem bacteria（B）and incidence rate（C）within 24 days of inoculation of resistant accession TK083 and susceptible accession Moneymaker（Mm）seedlings 5 days after inoculation with Ralstonia solanacearum t-Test，** indicates significantly difference at P < 0.01 level

Fig. 5 Changes of physiological indexes in seedlings of resistant accession TK083 and susceptible accession Moneymaker（Mm）after inoculation with Ralstonia solanacearum Significant difference analysis used Duncan’s test，different lowercase letters indicate significant differences in the same accession at different times（P < 0.05）

Fig. 6 Expression patterns of related immune response genes in resistant accession TK083 and susceptible accession Moneymaker（Mm）seedlings 5 days after inoculation with Ralstonia solanacearum t-Test，* indicates significantly difference at P < 0.05 level，** indicates significantly difference at P < 0.01 level，ns indicates no significant difference

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Screening and Identification for Bacterial Wilt Resistance Accession TK083 in Tomato

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