Genetic Diversity Analysis and Fingerprint Construction of Rosa Germplasm Resources Based on SSR Marker

doi:10.16420/j.issn.0513-353x.2024-0433

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (6): 1539-1552.doi: 10.16420/j.issn.0513-353x.2024-0433

• Genetic & Breeding?Germplasm Resources?Molecular Biology • Previous Articles Next Articles

Genetic Diversity Analysis and Fingerprint Construction of Rosa Germplasm Resources Based on SSR Marker

SUN Pei¹^,²^,³(), ZHANG Hong¹^,²^,³, YANG Yuan¹^,²^,⁴, WANG Hua¹^,²^,³, LI Maofu¹^,²^,³, KANG Yanhui¹^,²^,³, SUN Xiangyi¹^,²^,³, JIN Wanmei¹^,²^,³^,^*

¹ Institute of Forestry and Pomology，Beijing Academy of Agriculture and Forestry Sciences，Beijing 100093，China
² Key Laboratory of Biology and Genetic Improvement of Horticultural Crops（North China），Ministry of Agriculture and Rural Affairs，Beijing 100093，China
³ Beijing Engineering Research Center of Functional Floriculture，Beijing 100093，China
⁴ Beijing Engineering Research Center for Deciduous Fruit Trees，Beijing 100093，China

Received:2024-12-19 Revised:2025-02-28 Online:2025-06-20 Published:2025-06-20
Contact: JIN Wanmei

Abstract

Abstract:

15 SSR markers were adopted to analyze the genetic diversity and 3 core SSR markers established fingerprint of 80 rose germplasm resources. The ploidy level of some germplasm resources were also detected. The results showed that a total of 425 alleles were amplified by 15 SSR markers，with an average of 28.33 alleles per pair of primers and the number of alleles range from 7-64. The average number Polymorphic information content（PIC）was 83.52%，and the average power of discrimination（PD）was 84.84%. These parameters manifested that the tested resources got great genetic diversity. The similarity coefficient ranged from 0.27 to 0.67 among the 80 germplasm resources. Unweighted pair-group method with arithmetic means（UPGMA）analysis clustered all germplasm resources into three group at the similarity coefficient 0.28，and further divided the rose into two subgroups as Rosa hybrida and Chinese old rose when the similarity coefficient was 0.30. Three pairs of core SSR markers were selected to construct fingerprint. A certain correlation between the ploidy level and the number of amplified fragments for germplasm resources was identified，which means the number of amplified fragments could assist ploidy detection to some extent.

Key words: Rosa, SSR, genetic diversity, cluster analysis, fingerprint, ploidy test

SUN Pei, ZHANG Hong, YANG Yuan, WANG Hua, LI Maofu, KANG Yanhui, SUN Xiangyi, JIN Wanmei. Genetic Diversity Analysis and Fingerprint Construction of Rosa Germplasm Resources Based on SSR Marker[J]. Acta Horticulturae Sinica, 2025, 52(6): 1539-1552.

Figures/Tables 8

References 33

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原产地 Origin	品种群 Group	种质资源 Germplasm resources	原产地 Origin	品种群 Group	种质资源 Germplasm resources
中国 China	中国月季 China roses	绿萼 Rosa hybrida‘Lü’e’	德国 German	丰花月季 Floribunda roses	曼海姆宫殿 R. hybrida‘Schloss Mannheim’
	杂交茶香月季 Hybrid tea roses	绿野 R. hybrida‘Lüye’			德国黄金 R. hybrida‘Sunsprite’
		贵夫人R. hybrida‘Guifuren’		杂交茶香月季Hybrid tea roses	皇家巴西诺 R. hybrida‘Royal Bassino’
		兰星R. hybrida‘Lanxing’			月季夫人R. hybrida‘Lady Rose’
		长梦R. hybrida‘Big Dream’			红和平R. hybrida‘Red Peace’
		白荷R. hybrida‘Baihe’			电子表R. hybrida‘Funkuhr’
	丰花月季 Floribunda roses	红从容R. hybrida‘Red Velet’			热带雨林R. hybrida‘Tropical Rain’
		夏令营R. hybrida‘Xialingying’			金色霍尔斯R. hybrida‘Adolf Horstmann’
		红帽子R. hybrida‘Red Cap’			澳洲黄金 R. hybrida‘Australian Gold’
		红玫瑰王R. hybrida ‘Hongmeiguiwang’			莱茵黄金 R. hybrida‘Pfalzer Gold’
		黄玫瑰王R. hybrida‘Huang Meiguiwang’			蓝和平 R. hybrida‘Blue Peace’
		紫雾凝霜R. hybrida ‘Ziwuningshuang’			假日公主 R. hybrida‘Queensday’
		天坛白R. hybrida‘Tiantanbai’			蓝月R. hybrida‘Blue Moon’
	藤本月季 Climbing roses	读书台R. hybrida‘Dushutai’			蓝月亮R. hybrida‘Blue Monday’
		九重香 R. hybrida‘Everest Double’		藤本月季 Climbing roses	藤和谐 R. hybrida‘Rattan Harmonious’
	微型月季 Miniature roses	自由黄金 R. hybrida‘Ziyouhuangjin’			藤彩云 R. hybrida‘Rosy Cheeks’
		紫色时代 R. hybrida‘Ziseshidai’	日本 Japan	杂交茶香月季 Hybrid tea roses	绯扇R. hybrida‘Hiogi’
	玫瑰R. rugosa	玫瑰6号R. rugosa‘Meigui 6’			微笑R. hybrida‘Risa’
		玫瑰3号 R. rugosa‘Meigui 3’		丰花月季 Floribunda roses	花房R. hybrida‘Hanabusa’
		食用玫瑰R. rugosa‘Shiyongmeigui’			花车R. hybrida‘Hanaguruma’
		四季玫瑰 R. rugosa‘Sijimeigui’	荷兰 Netherlands	微型月季 Miniature roses	红柯斯特 R. Hybrida‘Red Margo Koster’
		平阴玫瑰R. rugosa‘Pinyinmeigui’			橙柯斯特 R. hybrida‘Margo Koster’
	蔷薇Rosa sp.	秦岭蔷薇R. tsinglingensis			白柯斯特R. hybrida‘Witte Koster’
澳大利亚Australian	杂交茶香月季Hybrid tea roses	阳光灿烂 R. hybrida‘Sunny Days’			柯斯特R. hybrida‘Koster’
保加利亚Bulgaria	玫瑰R. rugosa	大马士革玫瑰 R. rugosa damascena		藤本月季 Climbing roses	橘红火焰 R. hybrida‘Organe Fire’
丹麦 Denmark	杂交茶香月季Hybrid tea roses	英格丽褒曼 R. hybrida‘Ingrid Bergman’			橘红潮 R. hybrida‘Orange Wave’
丹麦 Denmark		肯特R. hybrida‘Ground Cover’			藤金凤凰 R. hybrida‘Golden Scepter’
法国 France	杂交茶香月季Hybrid tea roses	摩纳哥公主 R. hybrida‘Princesse de’Monaco’	英国 UK	藤本月季 Climbing roses	海格瑞R. hybrida‘Highgrove’
		大丰收R. hybrida‘Grand Gala’			欢乐格鲁吉亚 R. hybrida‘Teasing Georgia’
		卡罗拉R. hybrida‘Carola’		丰花月季 Floribunda roses	杏花村R. hybrida‘Betty Prior’
		美多斯R. hybrida‘Meduse’		杂交茶香月季Hybrid tea roses	希腊之乡 R. hybrida‘Sheilas Perfume’
	丰花月季 Floribunda roses	莫妮卡 R. hybrida‘Monique Darve’	美国 USA	杂交茶香月季Hybrid tea roses	墨绒 R. hybrida‘Mirandy’
		金色小兔子 R. hybrida‘Gold Bunny’			杏黄天 R. hybrida‘Apriot Nectar’
	藤本月季 Climbing roses	仙境 R. hybrida‘Carefree Wonder’	美国 USA	杂交茶香月季 Hybrid tea roses	金奖章 R. hybrida‘Gold Medal’
		红色龙沙宝石 R. hybrida‘Red Eden’			香妃 R. hybrida‘Sweet Fragrance’
		龙沙 R. hybrida‘Pierre de Ronasard’			白玫瑰王 R. hybrida‘White King’
		藤和平 R. hybrida‘Climbing Peace’		藤本月季 Climbing roses	藤金玛丽 R. hybrida‘Bridal White’
		白龙沙 R. hybrida‘Blanc Pierre de’		微型月季 Miniature roses	金太阳 R. hybrida‘Golden Sunblaze’
新西兰 New Zealand	杂交茶香月季 Hybrid tea roses	大紫光 R. hybrida‘Big Purple’		丰花月季 Floribunda roses	金不换 R. hybrida‘Kings Ransom’
爱尔兰 Ireland	杂交茶香月季 Hybrid tea roses	古龙 R. hybrida‘Kronenbourg’			黄蝴蝶 R. hybrida‘Yellow Butterfly’

原产地 Origin	品种群 Group	种质资源 Germplasm resources	原产地 Origin	品种群 Group	种质资源 Germplasm resources
中国 China	中国月季 China roses	绿萼 Rosa hybrida‘Lü’e’	德国 German	丰花月季 Floribunda roses	曼海姆宫殿 R. hybrida‘Schloss Mannheim’
	杂交茶香月季 Hybrid tea roses	绿野 R. hybrida‘Lüye’			德国黄金 R. hybrida‘Sunsprite’
		贵夫人R. hybrida‘Guifuren’		杂交茶香月季Hybrid tea roses	皇家巴西诺 R. hybrida‘Royal Bassino’
		兰星R. hybrida‘Lanxing’			月季夫人R. hybrida‘Lady Rose’
		长梦R. hybrida‘Big Dream’			红和平R. hybrida‘Red Peace’
		白荷R. hybrida‘Baihe’			电子表R. hybrida‘Funkuhr’
	丰花月季 Floribunda roses	红从容R. hybrida‘Red Velet’			热带雨林R. hybrida‘Tropical Rain’
		夏令营R. hybrida‘Xialingying’			金色霍尔斯R. hybrida‘Adolf Horstmann’
		红帽子R. hybrida‘Red Cap’			澳洲黄金 R. hybrida‘Australian Gold’
		红玫瑰王R. hybrida ‘Hongmeiguiwang’			莱茵黄金 R. hybrida‘Pfalzer Gold’
		黄玫瑰王R. hybrida‘Huang Meiguiwang’			蓝和平 R. hybrida‘Blue Peace’
		紫雾凝霜R. hybrida ‘Ziwuningshuang’			假日公主 R. hybrida‘Queensday’
		天坛白R. hybrida‘Tiantanbai’			蓝月R. hybrida‘Blue Moon’
	藤本月季 Climbing roses	读书台R. hybrida‘Dushutai’			蓝月亮R. hybrida‘Blue Monday’
		九重香 R. hybrida‘Everest Double’		藤本月季 Climbing roses	藤和谐 R. hybrida‘Rattan Harmonious’
	微型月季 Miniature roses	自由黄金 R. hybrida‘Ziyouhuangjin’			藤彩云 R. hybrida‘Rosy Cheeks’
		紫色时代 R. hybrida‘Ziseshidai’	日本 Japan	杂交茶香月季 Hybrid tea roses	绯扇R. hybrida‘Hiogi’
	玫瑰R. rugosa	玫瑰6号R. rugosa‘Meigui 6’			微笑R. hybrida‘Risa’
		玫瑰3号 R. rugosa‘Meigui 3’		丰花月季 Floribunda roses	花房R. hybrida‘Hanabusa’
		食用玫瑰R. rugosa‘Shiyongmeigui’			花车R. hybrida‘Hanaguruma’
		四季玫瑰 R. rugosa‘Sijimeigui’	荷兰 Netherlands	微型月季 Miniature roses	红柯斯特 R. Hybrida‘Red Margo Koster’
		平阴玫瑰R. rugosa‘Pinyinmeigui’			橙柯斯特 R. hybrida‘Margo Koster’
	蔷薇Rosa sp.	秦岭蔷薇R. tsinglingensis			白柯斯特R. hybrida‘Witte Koster’
澳大利亚Australian	杂交茶香月季Hybrid tea roses	阳光灿烂 R. hybrida‘Sunny Days’			柯斯特R. hybrida‘Koster’
保加利亚Bulgaria	玫瑰R. rugosa	大马士革玫瑰 R. rugosa damascena		藤本月季 Climbing roses	橘红火焰 R. hybrida‘Organe Fire’
丹麦 Denmark	杂交茶香月季Hybrid tea roses	英格丽褒曼 R. hybrida‘Ingrid Bergman’			橘红潮 R. hybrida‘Orange Wave’
丹麦 Denmark		肯特R. hybrida‘Ground Cover’			藤金凤凰 R. hybrida‘Golden Scepter’
法国 France	杂交茶香月季Hybrid tea roses	摩纳哥公主 R. hybrida‘Princesse de’Monaco’	英国 UK	藤本月季 Climbing roses	海格瑞R. hybrida‘Highgrove’
		大丰收R. hybrida‘Grand Gala’			欢乐格鲁吉亚 R. hybrida‘Teasing Georgia’
		卡罗拉R. hybrida‘Carola’		丰花月季 Floribunda roses	杏花村R. hybrida‘Betty Prior’
		美多斯R. hybrida‘Meduse’		杂交茶香月季Hybrid tea roses	希腊之乡 R. hybrida‘Sheilas Perfume’
	丰花月季 Floribunda roses	莫妮卡 R. hybrida‘Monique Darve’	美国 USA	杂交茶香月季Hybrid tea roses	墨绒 R. hybrida‘Mirandy’
		金色小兔子 R. hybrida‘Gold Bunny’			杏黄天 R. hybrida‘Apriot Nectar’
	藤本月季 Climbing roses	仙境 R. hybrida‘Carefree Wonder’	美国 USA	杂交茶香月季 Hybrid tea roses	金奖章 R. hybrida‘Gold Medal’
		红色龙沙宝石 R. hybrida‘Red Eden’			香妃 R. hybrida‘Sweet Fragrance’
		龙沙 R. hybrida‘Pierre de Ronasard’			白玫瑰王 R. hybrida‘White King’
		藤和平 R. hybrida‘Climbing Peace’		藤本月季 Climbing roses	藤金玛丽 R. hybrida‘Bridal White’
		白龙沙 R. hybrida‘Blanc Pierre de’		微型月季 Miniature roses	金太阳 R. hybrida‘Golden Sunblaze’
新西兰 New Zealand	杂交茶香月季 Hybrid tea roses	大紫光 R. hybrida‘Big Purple’		丰花月季 Floribunda roses	金不换 R. hybrida‘Kings Ransom’
爱尔兰 Ireland	杂交茶香月季 Hybrid tea roses	古龙 R. hybrida‘Kronenbourg’			黄蝴蝶 R. hybrida‘Yellow Butterfly’

引物名称 Primer name	引物序列（5′-3′） Primer sequence	退火温度/℃ T_m	来源 Source
CH01E12	F：AAACTGAAGCCATGAGGGC；R：TTCCAATTCACATGAGGCTG	60	James et al.，2004
CH04C07	F：GGCCTTCCATGTCTCAGAAG；R：CCTCATGCCCTCCACTAACA	59	Falginella et al.，2015
CH03H06	F：TTGTCCCTTTTTACGTCTTTCC；R：GTTATTGAGCAAGGCGGAGA	59	Naik et al.，2006
CH02H11A	F：CGTGGCATGCCTATCATTTG；R：CTGTTTGAACCGCTTCCTTC	62	Erfani-Moghadam & Zarei，2018
CH05H12	F：TTGCGGAGTAGGTTTGCTTT；R：TCAATCCTCATCTGTGCCAA	59	Naik et al.，2006
RH58	F：ACAATTTAGTGCGGATAGAACAAC；R：GGAAAGCCCGAAAGCGTAAGC	55	Yan et al.，2005
RH78	F：AAAGAAACGCGAAATCTATGATGC； R：TCTGGATGGGATTTAAAAGACAGG	56
RH93	F：GCTTTGCTGCATGGTTAGGTTG；R：TTCTTTTTGTCGTTCTGGGATGTG	57
RH96	F：GCCGATGGATGCCCTGCTC；R：AGATTCCCTGCGACATTCACATTC	60
RH98	F：GGCCTCTAGAGTTTGGGATAGCAG； R：ACGACGTCAATAACTCCATCAGTC	60
155SSR	F：GAAAAGAACGAGGGGTTTCC；R：ACGGTCGGTAATCAAGATGC	58	Biber et al.，2010
Rdr1RGA	F：TTTGCGTCTTTCTTGCTAA；R：CTTCTTCAGGCTCACATTTC	56	Biber et al.，2010
RH AB9-2	F：GTCAATTTGTGCATAAGCTC；R：GTGAGAACAGATGAGAAATG	55	Yan et al.，2005
RH AB38	F：GAGGTGGTCGATTCCATGTC；R：TTACCGTTCTACCTAAGTGACTAAC	57
RH ABT12	F：CAAGTTTGTCTCCTTGGACC；R：CATAGATGATTATCCTAGAGCC	55

引物名称 Primer name	引物序列（5′-3′） Primer sequence	退火温度/℃ T_m	来源 Source
CH01E12	F：AAACTGAAGCCATGAGGGC；R：TTCCAATTCACATGAGGCTG	60	James et al.，2004
CH04C07	F：GGCCTTCCATGTCTCAGAAG；R：CCTCATGCCCTCCACTAACA	59	Falginella et al.，2015
CH03H06	F：TTGTCCCTTTTTACGTCTTTCC；R：GTTATTGAGCAAGGCGGAGA	59	Naik et al.，2006
CH02H11A	F：CGTGGCATGCCTATCATTTG；R：CTGTTTGAACCGCTTCCTTC	62	Erfani-Moghadam & Zarei，2018
CH05H12	F：TTGCGGAGTAGGTTTGCTTT；R：TCAATCCTCATCTGTGCCAA	59	Naik et al.，2006
RH58	F：ACAATTTAGTGCGGATAGAACAAC；R：GGAAAGCCCGAAAGCGTAAGC	55	Yan et al.，2005
RH78	F：AAAGAAACGCGAAATCTATGATGC； R：TCTGGATGGGATTTAAAAGACAGG	56
RH93	F：GCTTTGCTGCATGGTTAGGTTG；R：TTCTTTTTGTCGTTCTGGGATGTG	57
RH96	F：GCCGATGGATGCCCTGCTC；R：AGATTCCCTGCGACATTCACATTC	60
RH98	F：GGCCTCTAGAGTTTGGGATAGCAG； R：ACGACGTCAATAACTCCATCAGTC	60
155SSR	F：GAAAAGAACGAGGGGTTTCC；R：ACGGTCGGTAATCAAGATGC	58	Biber et al.，2010
Rdr1RGA	F：TTTGCGTCTTTCTTGCTAA；R：CTTCTTCAGGCTCACATTTC	56	Biber et al.，2010
RH AB9-2	F：GTCAATTTGTGCATAAGCTC；R：GTGAGAACAGATGAGAAATG	55	Yan et al.，2005
RH AB38	F：GAGGTGGTCGATTCCATGTC；R：TTACCGTTCTACCTAAGTGACTAAC	57
RH ABT12	F：CAAGTTTGTCTCCTTGGACC；R：CATAGATGATTATCCTAGAGCC	55

引物名称 Primer name	等位基因 N_a	多态性比率/% Ratio of polymorphic alleles	产物大小/bp Production size	多态性信息量/% PIC	区分系数/% PD
CH01E12	31	100	64 ~ 394	82.29	83.06
CH04C07	20	100	51 ~ 386	80.89	82.92
CH03H06	29	100	43 ~ 454	72.29	74.31
CH02H11A	13	100	87 ~ 405	74.16	77.52
CH05H12	22	100	60 ~ 478	73.43	76.63
RH58	64	100	218 ~ 426	96.33	96.09
RH78	24	100	116 ~ 389	87.45	88.41
RH93	18	100	243 ~ 453	83.65	84.86
RH96	38	100	107 ~ 405	89.54	90.07
RH98	34	100	134 ~ 228	92.98	93.31
155 SSR	35	100	99 ~ 457	87.35	88.28
Rdr1 RGA	7	100	397 ~ 403	67.58	70.15
RH AB9-2	24	100	89 ~ 142	89.79	90.49
RH AB38	29	100	104 ~ 414	84.90	85.76
RH ABT12	37	100	153 ~ 275	90.21	90.72
合计 Total	425
平均 Average	28.33			83.52	84.84

Genetic Diversity Analysis and Fingerprint Construction of Rosa Germplasm Resources Based on SSR Marker

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品种 Cultivar	指纹图谱代码 Fingerprint codes	品种 Cultivar	指纹图谱代码 Fingerprint codes
绿萼 R. hybrida‘Lü’e’	A251/293	曼海姆宫殿 R. hybrida ‘Schloss Mannheim’	A251/289/360-B154 /176-C115/346/393
绿野 R. hybrida‘Lüye’’	A255/294/332-B170 /230-C115/202/290/393	皇家巴西诺 R. hybrida‘Royal Bassino’	A244/373-B155/226-C115/279/393
红从容 R. hybrida‘Red Velet’	A292/364-B154-C115/328/393	藤和谐 R. hybrida‘Rattan Harmonious’	A243/368-B168/197/224-C114/332
夏令营 R. hybrida‘Xialingying’	A274/368-B158/177-C115/347/392	月季夫人 R. hybrida‘Lady Rose’	A268/367-B168
贵夫人 R. hybrida‘Guifuren’	A232/293-B268/223-C115/392	红和平 R. hybrida‘Red Peace’	A267/395-C115/353
读书台 R. hybrida‘Dushutai’	A232/269/368-B167/227-C114/332	电子表 R. hybrida‘Funkuhr’	A256/293/359-B154/167-C115
九重香 R. hybrida‘Everest Double’	A243/366-C115	热带雨林 R. hybrida‘Tropical Rain’	A232/293/357-C115
红帽子 R. hybrida‘Red Cap’	A280/375-B154/228-C114	藤彩云 R. hybrida‘Rosy Cheeks’	A293/365-B154/168-C115/280
红玫瑰王 R. hybrida‘Hongmeiguiwang’	A230/290/362-C115	德国黄金 R. hybrida‘Sunsprite’	A251/365-B153-C115/352
自由黄金 R. hybrida‘Ziyouhuangjin’	A243/292/363-B154/235-C137/393	金色霍尔斯 R. hybrida‘Adolf Horstmann’	A254/292/357-B157/177-C115/394
黄玫瑰王 R. hybrida‘Huang Meiguiwang’	A251/365-B153/202-C115	澳洲黄金 R. hybrida‘Australian Gold’	A268/365-B154-C114/279/393
长梦 R. hybrida‘Big Dream’	A251/292/363-B167-C115	莱茵黄金 R. hybrida‘Pfalzer Gold’	A274/369/423-B154/274-C115
紫色时代 R. hybrida‘Ziseshidai’	A255/293/358-B155/168/223-C115/393	蓝和平 R. hybrida‘Blue Peace’	A293/366-B154-C115
紫雾凝霜 R. hybrida‘Ziwuningshuang’	A274/339-B166-C115	假日公主 R. hybrida‘Queensday’	A292/362-B166-C115
兰星 R. hybrida‘Lanxing’	A243/332-B168-C115	蓝月 R. hybrida‘Blue Moon’	A255/357-B167-C114/279
天坛白 R. hybrida‘Tiantanbai’	A254-B154/166/176-C115/347/393	蓝月亮 R. hybrida‘Blue Monday’	A262/291/354-B168-C114
白荷R. hybrida‘Baihe’	A291/364-B176/232-C115/279/332/392	绯扇R. hybrida‘Hiogi’	A232/293/357-C115/209/279/375
玫瑰6号 R. rugosa‘Meigui6hao’	A219/293-B167-C124/280/333/390	微笑 R. hybrida‘Risa’	A253/293/363-B154/223 -C115/230/279/353/391
玫瑰3号 R rugosa‘Meigui3hao’	A218/319/343-B173-C280/344	花房 R. hybrida‘Hanabusa’	A255/292/357-B167/225-C115/392
食用玫瑰 R rugosa‘Shiyongmeigui’	A219/293-B175/203/229-C280/344	花车 R. hybrida‘Hanaguruma’	A230/292-B154/176-C115
四季玫瑰 R rugosa‘Sijimeigui’	A219/293-B175/188/202/228-C280/333	红柯斯特 R. hybrida‘Red Margo Koster’	A268/366-B154-C115/332
平阴玫瑰 R rugosa‘Pinyinmeigui’	B172-C280/344	橘红火焰 R. hybrida‘Organe Fire’	A232/365-B176-C115
秦岭蔷薇R. tsinglingensis	A277/387-B171-C92/114	橘红潮 R. hybrida‘Orange Wave’	A243/271-B275
阳光灿烂 R. hybrida‘Sunny Days’	A269/367-B177/234-C115/206/279/393	橙柯斯特 R. hybrida‘Margo Koster’	A268/365-B154/168-C64/115/332
大马士革玫瑰 R. rugosa damascena	B175/189/202/228-C280	藤金凤凰 R. hybrida‘Golden Scepter’	A257/364-B154/177/225-C115/393
英格丽褒曼 R. hybrida‘Ingrid Bergman’	A292/363-B176-C115	白柯斯特 R. hybrida‘Witte Koster’	A268/366-B154/169/201
肯特R. hybrida‘Ground Cover’	A256-B154/197-C115/347/392	柯斯特R. hybrida‘Koster’	A293/365-B154/225-C94/115/393
摩纳哥公主 R. hybrida‘Princesse de’ Monaco’	A231/264-B154/168-C115	海格瑞 R. hybrida‘Highgrove’	A232-B154/167-C114/393
大丰收 R. hybrida‘Grand Gala’	A293/366-B177-C115/392	杏花村 R. hybrida‘Betty Prior’	A254/397-B176-C115
莫妮卡 R. hybrida‘Monique Darve’	A251/364-C115/393	希腊之乡 R. hybrida‘Sheilas Perfume’	A232/293/378-B167
仙境 R. hybrida‘Carefree Wonder’	A243/293/361-B154/176-C115/332/394	欢乐格鲁吉亚 R. hybrida‘Teasing Georgia’	A265/363-B154/177/202/232-C115
卡罗拉R. hybrida‘Carola’	A256/293/357-B154/224-C115/230	墨绒R. hybrida‘Mirandy’	A252/293/364-B167-C115
红色龙沙宝石 R. hybrida‘Red Eden’	B176-C115/346	杏黄天 R. hybrida‘Apriot Nectar’	A232/294-B154/176/225-C115/279
龙沙 R. hybrida‘Pierre de Ronasard’	A264/361-B177-C115	金奖章 R. hybrida‘Gold Medal’	A250/364-B154/176-C115/328
金色小兔子 R. hybrida‘Gold Bunny’	A250/365-B154/202-C115	藤金玛丽 R. hybrida‘Bridal White’	A293/363-B154/167-C115
藤和平 R. hybrida‘Climbing Peace’	A232/293-B154/177-C115	金太阳 R. hybrida‘Golden Sunblaze’	A233/276/389/426-B168
美多斯 R. hybrida‘Meduse’	A251/292/378-B154/228-C115	金不换 R. hybrida‘Kings Ransom’	A252/293/365-B168/235-C115/279/392
白龙沙 R. hybrida‘Blanc Pierre de’	A263/292/361-B176-C115	黄蝴蝶 R. hybrida‘Yellow Butterfly’	A256/356-B154-C115/353
大紫光 R. hybrida‘Big Purple’	A268/366-B153/230-C115 /202/328/352/393	香妃 R. hybrida‘Sweet Fragrance’	A293/365-B177-C115/328
古龙 R. hybrida‘Kronenbourg’	A231/292-B154/177-C115/353/392	白玫瑰王 R. hybrida‘White King’	A293/365-B176/231-C114/278

倍性 Ploidy	种质资源 Germplasm resources	最多扩增片段数量 Maximum number of amplified fragments	倍性 Ploidy	种质资源 Germplasm resources	最多扩增片段数量 Maximum number of amplified fragments
二倍体 Diploid	红柯斯特 R. hybrida Red Margo ‘Koster’	2	四倍体 Tetraploid	澳洲黄金 R. hybrida‘Australian Gold’	3
	肯特 R. hybrida‘Ground Cover’	3		黄玫瑰王 R. hybrida‘Huang Meiguiwang’	2
	天坛白 R. hybrida‘Tiantanbai’	3		金色小兔子 R. hybrida‘Gold Bunny’	2
	白柯斯特 R. hybrida‘Witte Koster’	3		莱茵黄金 R. hybrida‘Pfalzer Gold’	3
	杏花村 R. hybrida‘Betty Prior’	2		蓝和平 R. hybrida‘Blue Peace’	2
三倍体 Triploid	红帽子 R. hybrida‘Red Cap’	2		金太阳 R. hybrida‘Golden Sunblaze’	4
四倍体 Tetraploid	绿野 R. hybrida‘Lüye’	4		金不换 R. hybrida‘Kings Ransom’	3
	绯扇 R. hybrida‘Hiogi’	4		黄蝴蝶 R. hybrida‘Yellow Butterfly’	2
	阳光灿烂 R. hybrida‘Sunny Days’	4		白龙沙 R. hybrida‘Blanc Pierre de’	3
	曼海姆宫殿R. hybrida‘Schloss Mannheim’	3		香妃 R. hybrida‘Sweet Fragrance’	2
	仙境R. hybrida ‘Carefree Wonder’	3		美多斯 R. hybrida‘Meduse’	3
	卡罗拉 R. hybrida‘Carola’	3		蓝月 R. hybrida‘Blue Moon’	2
	橘红火焰 R. hybrida‘Organe Fire’	2		兰星 R. hybrida‘Lanxing’	2
	夏令营 R. hybrida‘Xialingying’	3		蓝月亮 R. hybrida‘Blue Moon’	3
	月季夫人 R. hybrida‘Lady Rose’	2		自由黄金 R. hybrida‘Ziyouhuangjin’	3
	九重香 R. hybrida‘Everest Double’	2		德国黄金 R. hybrida‘Sunsprite’	2
	龙沙R. hybrida‘Pierre de Ronasard’	2		金色霍尔斯 R. hybrida‘Adolf Horstmann’	3
	希腊之乡 R. hybrida‘Sheilas Perfume’	3		白玫瑰王 R. hybrida‘White King’	2
	杏黄天 R. hybrida‘Apriot Nectar’	3		白荷 R. hybrida‘Baihe’	4
	金奖章 R. hybrida‘Gold Medal’	2		大紫光 R. hybrida‘Big Purple’	5

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