Establishment of Agrobacterium-mediated Transformation in Phalaenopsis

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (4): 537-539.

• 研究报告 • Previous Articles Next Articles

Establishment of Agrobacterium-mediated Transformation in Phalaenopsis

Chai Mingliang and Kim Doohwan

( Department of Horticulture，Zhejiang University，Hangzhou 310029，China； Department of Horticultural Science，KonkukUniversity，Seoul 143—701，Korea)

Received:1900-01-01 Revised:1900-01-01 Online:2004-08-25 Published:2004-08-25

Abstract

Abstract: Transgenic orchid(Phalaenopsis CV．White Hikaru)plants were regenerated by inoculating a
needle wounded protocorm．1ike body (PLB)with Agrobacterium tumefaciens strain LBA4404 harboring
pCAMBIA 1300-SmGFP that carries green fluorescent protein gene( )and hygromycin phosphotransferase
gene( f)．The expression and presence of the transgenes， and f were assessed by detection of green
fluorescent protein under microscope and Southern analysis，respectively．

Key words: Phalaenopsis, Protocorm—like body(PLB), Agrobacterium．mediated transformation, Greenfluorescent protein gene( ), Hygromycin phosphotransferase gene( f)

Chai Mingliang and Kim Doohwan. Establishment of Agrobacterium-mediated Transformation in Phalaenopsis[J]. ACTA HORTICULTURAE SINICA, 2004, 31(4): 537-539.

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Establishment of Agrobacterium-mediated Transformation in Phalaenopsis

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