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ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (4): 487-490.

• 研究论文 • Previous Articles     Next Articles

RAPD Identification and Discrimination of 42 Ornamental Pink Double FormCultivars of P nus mume Sieb.et Zucc.

Zhang Junwei,Chai Yurong,and Bao Manzhu
  

  1. (Key Laboratory of Horticultural Plant Biology,Ministry of Education,College of Horticulture and Forestr) Sciences,HuazhongAgricultural University,Wuhan 430070,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-08-25 Published:2004-08-25

Abstract: The genomic DNA was used as template and random primers were used to develop fingerprint—
ing of 42 cuhivars of Pink Double Form of Pr“n aurae Sieb. et Zucc. by PCR. Of the 120 primers
screened.21 primers can generate 96 fragments,70.8% of which were polymorphic ones.The similarity c0efficient
of cultivars was between 0.737—0.96 1.The 42 cuhivars can be identified and discriminated eficiently
with seven primers. The establishment of RAPD fingerprinting is applicable in cuhivar identification and
breeder's rights protection of Pr“m aurae.

Key words: Prunus aurae, Pink Double Form, RAPD, Fingerprinting