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ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (4): 505-508.

• 研究报告 • Previous Articles     Next Articles

Leaf Disc Assay for Identification of Resistance of Strawberry to PowderyMildew and Screening of Fungicides

Zhang Zhihong,Uu Yan, Gao Xiuyan, Du Guodong, and Dai Hongyan   

  1. (College of Horticulture,Shenyang Agricultural University,Shenyang 1 10161,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-08-25 Published:2004-08-25

Abstract: The strawberry leaf discs, diameter 1 am ,were inoculated with the conidia of strawberry
powdery mildew (Sphaerotheca macular~)by spraying or shaking of,and then were transferred to Petri
plates containing wet filter paper. The leaf discs were cultured at 20℃ with a 1 6 h photoperiod at a light intensity
of 30O0 lX. The colonies on the inoculated leaf discs were observed clearly 7 days after inoculation.
The resistance of detached leaf discs of strawberry cultivars ‘Toyonoka’. ‘Akihime’ and ‘Allstar’ to pow—
dery mildew was consistent with the behavior of these cuhivars in field. The result of screening of fungicides
by leaf disc assay showed that flusilazol and myclobutanil were effective fungicides for control of powdery rail—
dew on strawberry.

Key words: Strawberry, Powdery mildew, Resistance, Identification, LeaLf disc