Heredity and Expression of Foreign Genes in pinⅡ Transgenic FloweringChinese Cabbage Obtained by Vacuum Infiltration

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (4): 511-513.

• 研究报告 • Previous Articles Next Articles

Heredity and Expression of Foreign Genes in pinⅡ Transgenic FloweringChinese Cabbage Obtained by Vacuum Infiltration

Xu Hengjian ’ ，Liu Fan ，Wang Xiufeng ，Zhao Hong ，Cao Chuanzeng ，and Luo Chen

( Collage ofHorticulture，Shandong Agricultural University，T~i'an 271018，China； National Engineering Research CenterforVegetables，Beijing 100089，China； Institute of Plant and Environmental Protection，Beijing Academy ofAgriculture and Forest—ry，Beijing 100089，China)

Received:1900-01-01 Revised:1900-01-01 Online:2004-08-25 Published:2004-08-25

Abstract

Abstract: Transgenic Chinese cabbage (Brassica campestris L．ssp．chinensis var．utilis Tsen et Lee)
with pin n gene and bar gene were got by vacuum infiltration transformation and strictly self-crossed．PCR assay，
Basta resistance and insect resistance of T2 generation lines proved stable inheritance and expression of the
foreign genes．The segregation ratios of 41 lines indicated low—copy transformation，and majority of them accord—
ing with the Mendel’S segregation law．The resistance of transgenic lines to Plutella xylostella L．has significant
diference along with the variation of relative quantities of PIN II protein．even lines with the san3．e insertion copies．

Key words: Flowering Chinese cabbage, pin n gene, Insect resistance, Gene expression

Xu Hengjian ’;Liu Fan;Wang Xiufeng;Zhao Hong;Cao Chuanzeng;and Luo Chen. Heredity and Expression of Foreign Genes in pinⅡ Transgenic FloweringChinese Cabbage Obtained by Vacuum Infiltration[J]. ACTA HORTICULTURAE SINICA, 2004, 31(4): 511-513.

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Heredity and Expression of Foreign Genes in pinⅡ Transgenic FloweringChinese Cabbage Obtained by Vacuum Infiltration

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