Studies on Cultivar Classification of Osmanthus fragrans by ISSR·PCR Analysis

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (4): 529-532.

• 研究报告 • Previous Articles Next Articles

Studies on Cultivar Classification of Osmanthus fragrans by ISSR·PCR Analysis

Qiu Yingxiong ，Hu Shaoqing ，Chen Yuelei ，Chen Xiao ，and Wu Guanghong

( College ofLife Sciences，Zhejiang University，Hangzhou 310029，China； Hangzhou Botanical Garden，Hangzhou 310013China； Hangzhou Sweet Olive Technology Development Co．，Ltd，Hangzhou 310010，China)

Received:1900-01-01 Revised:1900-01-01 Online:2004-08-25 Published:2004-08-25

Abstract

Abstract: In this study，inter．simple sequence repeat(ISSR)was evaluated for its potential use in the
identification of 1 9 Osmanthus fragran,s cuhivars．1 3 out of 74(1 8．6％)ISSR primers could generate repro．
ducible polymorphic fragments．The ISSR．PCR assay revealed a total number of 90 DNA bands．of which 79
bands were polymorphic(the percentage of polymorphic bands，PPB=87．8％)．Optimized ISSR primers
amplified 4 to 1 0 bands ranging in size from 300 bp to 2000 bp，with an overall average of 6．92 amplified
bands per primer．RAPDistance software was used to calculate the Nei’S genetic distance and a dendrogram
was constructed based on UPGMA cluster analysis．These 1 9 Osmanthus fragrans cuhivars surveyed were clas．
sifted into eight major groups，and each cuhivar in this study could be distinguished from others．The genetic
relationship of four cuhivars group and classification of Osmanthus fragrans were also discussed．

Key words: Osmanthus fragrans, Cuhivars, ISSR, Genetic relationship

Qiu Yingxiong;Hu Shaoqing;Chen Yuelei;Chen Xiao;and Wu Guanghong. Studies on Cultivar Classification of Osmanthus fragrans by ISSR·PCR Analysis[J]. ACTA HORTICULTURAE SINICA, 2004, 31(4): 529-532.

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Studies on Cultivar Classification of Osmanthus fragrans by ISSR·PCR Analysis

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