园艺学报 ›› 2021, Vol. 48 ›› Issue (5): 849-859.doi: 10.16420/j.issn.0513-353x.2020-0895

• 研究论文 •    下一篇


姚富文1,*, 王枚阁1,*, 宋春晖1, 宋尚伟1, 焦健1, 王苗苗1, 王昆2, 白团辉1,**(), 郑先波1,**()   

  1. 1河南农业大学园艺学院,郑州 450002
    2中国农业科学院果树研究所,辽宁兴城 125100
  • 收稿日期:2020-12-29 修回日期:2021-04-08 出版日期:2021-05-25 发布日期:2021-06-07
  • 通讯作者: 白团辉,郑先波;
  • 作者简介:*共同第一作者
  • 基金资助:

Identification and Expression Analysis of HSP90 Gene Family Under High Temperature Stress in Apple

YAO Fuwen1,*, WANG Meige1,*, SONG Chunhui1, SONG Shangwei1, JIAO Jian1, WANG Miaomiao1, WANG Kun2, BAI Tuanhui1,**(), ZHENG Xianbo1,**()   

  1. 1College of Horticulture,Henan Agricultural University,Zhengzhou 450002,China
    2Institute of Pomology,Chinese Academy of Agricultural Sciences,Xingcheng,Liaoning 125100,China
  • Received:2020-12-29 Revised:2021-04-08 Online:2021-05-25 Published:2021-06-07
  • Contact: BAI Tuanhui,ZHENG Xianbo;


为了探究热激蛋白(HSP90)基因家族成员在苹果(Malus × domesticaBorkh.)耐热中的作用,利用生物信息学方法对苹果HSP90基因家族成员进行鉴定,并分析其在高温胁迫下和不同组织中的表达规律。结果表明,苹果HSP90基因家族有11个成员,分布于10条染色体上,其氨基酸序列大小介于104 ~ 818 aa,蛋白质分子质量介于11.81 ~ 93.58 kD。转录组分析显示,11个MdHSP90家族成员中,有4个基因,MdHSP90-1MdHSP90-3MdHSP90-5MdHSP90-11在高温胁迫下显著上调;亚细胞定位显示这4个基因中前三者定位在细胞膜与细胞核上,而后者定位在细胞核上;进一步通过qRT-PCR分析发现这4个基因在两种苹果砧木平邑甜茶(Malus hupehensis)和变叶海棠(Malus toringoides)中的表达趋势基本一致,其表达量在高温胁迫早期大幅升高,在中后期有所下降;不同苹果组织中均能检测到这4个基因的表达,高温处理可不同程度地激活这些基因的表达,表明它们可能在苹果耐热胁迫中具有重要作用。

关键词: 苹果, HSP90, 高温, 基因, 结构, 基因表达


In order to explore the role of heat shock protein(HSP90)gene family members in heat tolerance of apple(Malus × domestica Borkh.),bioinformatics method was used to identify the apple HSP90 gene family members,and their physicochemical properties,gene structure,cis elements,phylogenetic relationship and expression characteristics of HSP90 gene family under high temperature stress as well as in different tissues were analyzed. The results showed that a total of 11 HSP90 family members were identified in apple genome,which were distributed on 10 chromosomes. The amino acid sequence size was ranged from 104 to 818 aa,and molecular weight of protein ranged from 11.81 to 93.58 kD. Transcriptome analysis showed that four genes(MdHSP90-1,MdHSP90-3,MdHSP90-5and MdHSP90-11)of the 11 members were significantly up-regulated under high temperature stress. Subcellular localization analysis showed that MdHSP90-1,MdHSP90-3 and MdHSP90-5 proteins were located in the cell cytoplasm and nucleus,whereas MdHSP90-11 was targeted to the nucleus. Subsequent qRT-PCR analysis showed that the expression trend of the four genes in the two kinds of apple rootstocks were similar,the expression level increased greatly in the early stage of high temperature stress,and decreased in the middle and late stage. Four genes were also detected in different apple tissues,and the expressions of fourHSP90 genes were induced by heat stress to varying degrees,which indicated that the above four HSP90 genes may play an important role in the heat tolerance of apple.

Key words: apple, HSP90, high temperature stress, gene, structure, gene expression