园艺学报 ›› 2023, Vol. 50 ›› Issue (1): 65-78.doi: 10.16420/j.issn.0513-353x.2021-0985

• 研究论文 • 上一篇    下一篇


崔建1,2, 钟雄辉2, 刘泽慈2, 陈登辉2, 李海龙2, 韩睿2, 乐祥庆2, 康俊根2,*(), 王超1,*()   

  1. 1东北农业大学园艺园林学院,哈尔滨 150030
    2北京市农林科学院蔬菜研究所,农业农村部华北地区园艺作物生物学与种质创制重点实验室,北京 100097
  • 收稿日期:2022-08-17 修回日期:2022-11-17 出版日期:2023-01-25 发布日期:2023-01-18
  • 通讯作者: *(
  • 基金资助:

Construction of Cabbage Chromosome Segment Substitution Lines

CUI Jian1,2, ZHONG Xionghui2, LIU Zeci2, CHEN Denghui2, LI Hailong2, HAN Rui2, YUE Xiangqing2, KANG Jungen2,*(), WANG Chao1,*()   

  1. 1School of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150030,China
    2Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture,Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China
  • Received:2022-08-17 Revised:2022-11-17 Online:2023-01-25 Published:2023-01-18
  • Contact: *(


以结球甘蓝(Brassica oleracea L. var. capitata)育种骨干亲本R2-P2(圆球形,抽薹晚,硫苷含量低,高感黑腐病、枯萎病和霜霉病)为轮回亲本,以野生甘蓝自交系R4-P1(不结球,抽薹早,蜡质厚,硫苷含量高,对黑腐病、枯萎病和霜霉病等多种病害具有抗性)为供体亲本,通过连续回交、自交和分子标记辅助选择,构建出一套由163个具有R2-P2遗传背景且具有R4-P1供体染色体片段单株组成的染色体片段替换系(Chromosome segment substitution line)。该替换系群体具有102个染色体替换片段,均匀分布在1 ~ 9号染色体上,平均每个株系携带1.3个染色体片段,平均替换长度为5.62 Mb。替换片段总长度为573.74 Mb,覆盖亲本R4-P1全基因组的73.4%,其中单片段替换片段总长度为139.91 Mb,覆盖全基因组的26.5%,双片段替换片段总长231.41 Mb,覆盖亲本R4-P1全基因组的43.82%,含有3个替换片段总长度为201.3 Mb,覆盖供体亲本基因组25.98%。

关键词: 结球甘蓝, 染色体片段替换系, 全基因组, 分子标记, QTL定位


In this experiment,cabbage R2-P2,a foundation parent with head,late bolting and low glucosinolate content,high susceptibility to black rot,Fusarium wilt and downy mildew was served as the recurrent parent. On the contrary,the donor parent R4P1 is a wild cabbage inbred line R4-P1 with no-heading,early bolting,thick wax,high glucosinolate content,and resistance to black rot,Fusarium wilt,downy mildew and other diseases. Resultly,the Chromosome segment substitution lines(CSSLs)was both possessed the genetic background of R2-P2 and individual donor fragment of R4-P1. The CSSLs with 163 lines was constructed by continuous backcross,selfing and marker-assisted selection. The CSSLs contained 102 substituted segments totally,which were derived from genome of donor parent R4-P1 and evenly distributed on nine chromosomes. There were average 1.3 chromosome fragments on a line at least. The whole length of the substituted segments was 573.74 Mb with an average length of 5.62 Mb,which covered the 73.4% of complete DNA sample of R4-P1. Among them,139.91 Mb of single substituted segments covered the 26.5% of complete DNA. Besides,the double-segment replacement fragments were 231.41 Mb,which was accounted for 43.82%. Meanwhile,the total length of three replacement fragments was 201.3 Mb,covering 25.98% of the donor parent genome.

Key words: cabbage, Chromosome segment substitution line, genome, molecular marker, QTL