侵染鸢尾的番茄斑萎病毒鉴定

doi:10.16420/j.issn.0513-353x.2021-0806

摘要/Abstract

摘要：

2020年，在云南中医药大学校园发现叶片表现褪绿斑点、坏死环斑症状的鸢尾植株。用电子显微镜在病叶汁液中观察到典型的正番茄斑萎病毒属（Orthotospovirus）病毒粒子。在用感病叶片汁液摩擦接种的普通烟、本生烟上观察到系统坏死斑症状，中国辣椒（PI152225）的接种叶片出现局部坏死斑，但无系统侵染。为进一步明确病原，用RT-PCR扩增番茄斑萎病毒（tomato spotted wilt orthotospovirus，TSWV）的NSs和N基因，序列测定发现，分别与分离自云南番茄和旱金莲的TSWV分离物具有最高的氨基酸序列相似性（99.79%和100%）。结果表明引起鸢尾褪绿斑点病的病原是番茄斑萎病毒。

关键词: 鸢尾, 番茄斑萎病毒, N基因, NSs基因

Abstract:

In 2020，Iris tectorum Maxim plants exhibiting chlorotic spot and necrotic ringspot symptoms were observed on campus in Yunnan University of Chinese Medicine in Kunming City，Yunnan Province. The typical virions of orthotospoviruses were observed by electron microscope. The systemic necrotic spots symptoms were found on the plants of Nicotiana tabacum and N. benthamiana inoculated mechanically with the sap of the symptomatic I.tectorum Maxim leaves. The inoculated leaves of Capsicum chinense Jacquin PI152225 showed the local lethal spots，whilst no systemic infection were detected. To further confirm the pathogen，RT-PCR were performed to amplify the NSs and N genes of tomato spotted wilt orthotospovirus（TSWV），respectively. Sequence analysis showed the obtained sequences had the highest identities（99.79% and 100%）with NSs and N genes of TSWV isolates infecting tomato and Tropaeolum majus in Yunnan Province. The results indicated TSWV infection causes the chlorotic spots and necrotic ringspots sympt，oms in I. tectorum.

Key words: Iris tectorum, tomato spotted wilt orthotospovirus, N gene, NSs gene

中图分类号:

S682.19

忽靖宇, 阙开娟, 缪田丽, 吴少政, 王田田, 张磊, 董鲜, 季鹏章, 董家红. 侵染鸢尾的番茄斑萎病毒鉴定[J]. 园艺学报, 2023, 50(1): 170-176.

HU Jingyu, QUE Kaijuan, MIAO Tianli, WU Shaozheng, WANG Tiantian, ZHANG Lei, DONG Xian, JI Pengzhang, DONG Jiahong. Identification of Tomato Spotted Wilt Orthotospovirus Infecting Iris tectorum[J]. Acta Horticulturae Sinica, 2023, 50(1): 170-176.

图/表 3

图1 自然感病鸢尾症状和电子显微镜观察到的病毒粒子 A:发病早期的褪绿斑；B:发病后期坏死环斑；C:蒴果上的坏死和褪绿斑；D:中国辣椒PI152225接种叶局部坏死斑；E:病毒粒子。

Fig. 1 Symptoms of naturally diseased iris and viral particles under the electron microscope A. Chlorotic spot at early state of infection；B. Necrotic ringspot at late state of infection；C. Chlorotic and necrotic spots on the capsule；D. Local lethal spots on the inoculated leaves of Capsicum chinense Jacquin PI152225；E. Micrograph of virus particles.

图2 鸢尾样品中TSWV的N和NSs基因扩增 M:DL2000 marker；1:健康对照；2 ~ 5:感病鸢尾样品。

Fig. 2 Amplification of N and NSs genes of TSWV-ITM isolate M:DL2000 DNA marker；1:Healthy control；2-5:Diseased iris samples.

图3 基于TSWV鸢尾分离物和其他TSWV分离物的N蛋白氨基酸序列构建的系统进化树

Fig. 3 Phylogenetic tree based on the N protein aa sequences of TSWV-ITM isolate and other TSWV isolates

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