园艺学报 ›› 2023, Vol. 50 ›› Issue (1): 1-14.doi: 10.16420/j.issn.0513-353x.2021-0932
• 研究论文 • 下一篇
翟含含1,*, 翟宇杰1,*, 田义2(), 张叶1, 杨丽1, 温陟良1,**(), 陈海江1,**()
收稿日期:
2022-07-15
修回日期:
2022-11-16
出版日期:
2023-01-25
发布日期:
2023-01-18
通讯作者:
**(E-mail:作者简介:
*共同第一作者
基金资助:
ZHAI Hanhan1,*, ZHAI Yujie1,*, TIAN Yi2(), ZHANG Ye1, YANG Li1, WEN Zhiliang1,**(), CHEN Haijiang1,**()
Received:
2022-07-15
Revised:
2022-11-16
Online:
2023-01-25
Published:
2023-01-18
Contact:
**(E-mail:摘要:
为了探究SAUR(Small Auxin-Up RNA)基因家族成员在桃树(Prunus persica)生长发育中的作用,利用生物信息学方法对桃SAUR家族成员(PpSAUR)进行鉴定,对其染色体定位、基因结构、保守元件、进化关系及基因表达等进行分析,并对PpSAUR5功能进行初步鉴定。结果表明:桃中共有80个SAUR成员,分为12个亚组,不均匀分布在8条染色体上;基因结构显示75个PpSAUR只有1个外显子,余下5个有2 ~ 3个外显子;通过分析不同树势(强、中、弱)桃品种的转录组获得18个与树体长势呈正或负相关的SAUR基因,这些基因对外源植物生长调节物质处理响应不同。PpSAUR5对生长素与赤霉素均有响应,功能验证显示PpSAUR5转基因拟南芥幼苗叶柄、下胚轴及根较野生型长,且对NAA与2,4-D处理敏感性降低。研究结果表明PpSAUR5能够促进器官伸长。
中图分类号:
翟含含, 翟宇杰, 田义, 张叶, 杨丽, 温陟良, 陈海江. 桃SAUR家族基因分析及PpSAUR5功能鉴定[J]. 园艺学报, 2023, 50(1): 1-14.
ZHAI Hanhan, ZHAI Yujie, TIAN Yi, ZHANG Ye, YANG Li, WEN Zhiliang, CHEN Haijiang. Genome-wide Identification of Peach SAUR Gene Family and Characterization of PpSAUR5 Gene[J]. Acta Horticulturae Sinica, 2023, 50(1): 1-14.
基因Gene | 登录号Bank number | 引物序列(5'-3')Primer sequence |
---|---|---|
PpActin | Prupe.6G163400 | F:GCCCAAGTGCTTCGTATGCT;R:ATCACCGGCTGCAATCCA |
PpSAUR3 | Prupe.1G221300 | F:TCCGAGACGGGCTAAGAAGA;R:ATTCGGGTACGACAGCCAAG |
PpSAUR4 | Prupe.1G367800 | F:TTTGGAACTCAGGGCGAGTC;R:CAAGGCCTCCCTTTGGATGT |
PpSAUR5 | Prupe.1G368100 | F:ACGAAGCAACGGTCGAGAAT;R:CAGTTGCACCCGATTTGTGG |
PpSAUR10 | Prupe.2G140600 | F:TTTGGAACTCAGGGCGAGTC;R:CAAGGCCTCCCTTTGGATGT |
PpSAUR20 | Prupe.4G136800 | F:TTTGGAACCGTCACAGGCTT;R:CTCCTTGCAGCAACAAACCC |
PpSAUR26 | Prupe.6G108500 | F:ATGGAATGGTGATGCCACCC;R:TGGCTATGACGAATGTCCCAA |
PpSAUR34 | Prupe.7G167000 | F:CTTAAGAACCGCCCGGAGTT;R:AGTACAAGCCGAGGAGGAGT |
PpSAUR41 | Prupe.8G078600 | F:CAAGGGATTGTGAGGCCCAT;R:CTCAAACCGCAGTGCTCAAG |
PpSAUR42 | Prupe.8G078700 | F:GCTTCTTTTGGCTCTCCCCA;R:TCCGGTTGCCTGGAATTGTT |
PpSAUR46 | Prupe.8G079100 | F:AAACTGCGTCTTTTGGCTCC;R:TTGCCCGGAATTGTTAACGC |
PpSAUR47 | Prupe.8G079200 | F:AGGCATCTTCACTGCATGGG;R:GGAGAGCAAGAAGAAGCGGT |
PpSAUR56 | Prupe.8G080200 | F:ACATAGGAATGACGACCCGC;R:CCGTCGTCCTAGTAAGCAGC |
PpSAUR60 | Prupe.8G080600 | F:TGAAACTCTGGATGGCCCAG;R:AGATGTGCCAAAGGGGCATT |
PpSAUR64 | Prupe.8G081100 | F:TTCTCAAGCTCTCGTGGCTG;R:GTGCCGGAAGATGTGAAGGA |
PpSAUR73 | Prupe.8G082100 | F:GGCACCCAACTGAGAAGTGA;R:ACGAGGTTCCAAAAGGGCAT |
PpSAUR74 | Prupe.8G082200 | F:GCGGGAAGTGAGATGAAGGA;R:GGAGCCAAAAGACGCAGTTT |
PpSAUR77 | Prupe.8G157900 | F:TAATTGCTCTCCATCGCCCC;R:AGAGTTGCTAAGCGAGGCTG |
PpSAUR80 | Prupe.8G158200 | F:AGACGATCACGTGACAAGGG;R:CGTCGAGCTCCTGAACAAGT |
表1 qRT-PCR验证引物序列
Table 1 Primer used in qRT-PCR Validation
基因Gene | 登录号Bank number | 引物序列(5'-3')Primer sequence |
---|---|---|
PpActin | Prupe.6G163400 | F:GCCCAAGTGCTTCGTATGCT;R:ATCACCGGCTGCAATCCA |
PpSAUR3 | Prupe.1G221300 | F:TCCGAGACGGGCTAAGAAGA;R:ATTCGGGTACGACAGCCAAG |
PpSAUR4 | Prupe.1G367800 | F:TTTGGAACTCAGGGCGAGTC;R:CAAGGCCTCCCTTTGGATGT |
PpSAUR5 | Prupe.1G368100 | F:ACGAAGCAACGGTCGAGAAT;R:CAGTTGCACCCGATTTGTGG |
PpSAUR10 | Prupe.2G140600 | F:TTTGGAACTCAGGGCGAGTC;R:CAAGGCCTCCCTTTGGATGT |
PpSAUR20 | Prupe.4G136800 | F:TTTGGAACCGTCACAGGCTT;R:CTCCTTGCAGCAACAAACCC |
PpSAUR26 | Prupe.6G108500 | F:ATGGAATGGTGATGCCACCC;R:TGGCTATGACGAATGTCCCAA |
PpSAUR34 | Prupe.7G167000 | F:CTTAAGAACCGCCCGGAGTT;R:AGTACAAGCCGAGGAGGAGT |
PpSAUR41 | Prupe.8G078600 | F:CAAGGGATTGTGAGGCCCAT;R:CTCAAACCGCAGTGCTCAAG |
PpSAUR42 | Prupe.8G078700 | F:GCTTCTTTTGGCTCTCCCCA;R:TCCGGTTGCCTGGAATTGTT |
PpSAUR46 | Prupe.8G079100 | F:AAACTGCGTCTTTTGGCTCC;R:TTGCCCGGAATTGTTAACGC |
PpSAUR47 | Prupe.8G079200 | F:AGGCATCTTCACTGCATGGG;R:GGAGAGCAAGAAGAAGCGGT |
PpSAUR56 | Prupe.8G080200 | F:ACATAGGAATGACGACCCGC;R:CCGTCGTCCTAGTAAGCAGC |
PpSAUR60 | Prupe.8G080600 | F:TGAAACTCTGGATGGCCCAG;R:AGATGTGCCAAAGGGGCATT |
PpSAUR64 | Prupe.8G081100 | F:TTCTCAAGCTCTCGTGGCTG;R:GTGCCGGAAGATGTGAAGGA |
PpSAUR73 | Prupe.8G082100 | F:GGCACCCAACTGAGAAGTGA;R:ACGAGGTTCCAAAAGGGCAT |
PpSAUR74 | Prupe.8G082200 | F:GCGGGAAGTGAGATGAAGGA;R:GGAGCCAAAAGACGCAGTTT |
PpSAUR77 | Prupe.8G157900 | F:TAATTGCTCTCCATCGCCCC;R:AGAGTTGCTAAGCGAGGCTG |
PpSAUR80 | Prupe.8G158200 | F:AGACGATCACGTGACAAGGG;R:CGTCGAGCTCCTGAACAAGT |
基因名称 Gene name | 基因登录号 Gene ID | 染色体位置Chromosome location | 蛋白质Protein | |||
---|---|---|---|---|---|---|
起始位点/bp Start position | 终止位点/bp End position | 长度/aa Length | 分子量/D MW | 理论等点 pI | ||
PpSAUR1 | Prupe.1G000200 | 42 266 | 44 493 | 173 | 19 380.4 | 11.05 |
PpSAUR2 | Prupe.1G067400 | 4 810 025 | 4 811 236 | 126 | 14 192.5 | 8.63 |
PpSAUR3 | Prupe.1G221300 | 23 585 167 | 23 586 506 | 127 | 14 725.8 | 8.23 |
PpSAUR4 | Prupe.1G367800 | 33 658 384 | 33 660 037 | 126 | 14 253.5 | 9.14 |
PpSAUR5 | Prupe.1G368100 | 33 683 348 | 33 685 088 | 131 | 15 426.6 | 7.38 |
PpSAUR6 | Prupe.1G455700 | 38 251 559 | 38 252 129 | 127 | 14 616.0 | 8.15 |
PpSAUR7 | Prupe.2G131400 | 18 964 628 | 18 965 376 | 122 | 13 712.8 | 9.44 |
PpSAUR8 | Prupe.2G131500 | 18 966 838 | 18 967 203 | 98 | 11 226.7 | 7.22 |
PpSAUR9 | Prupe.2G131600 | 18 972 827 | 18 973 204 | 91 | 9 998.4 | 7.51 |
PpSAUR10 | Prupe.2G140600 | 19 729 097 | 19 730 272 | 105 | 11 631.2 | 7.50 |
PpSAUR11 | Prupe.2G194600 | 23 275 229 | 23 276 493 | 82 | 9 069.3 | 5.02 |
PpSAUR12 | Prupe.2G211600 | 24 387 357 | 24 388 174 | 84 | 9 269.6 | 9.19 |
PpSAUR13 | Prupe.2G317100 | 29 700 658 | 29 701 286 | 151 | 16 864.3 | 9.12 |
PpSAUR14 | Prupe.3G023900 | 1 785 030 | 1 786 044 | 78 | 8 619.9 | 8.98 |
PpSAUR15 | Prupe.3G024000 | 1 787 115 | 1 787 919 | 102 | 11 216.9 | 8.21 |
PpSAUR16 | Prupe.3G024100 | 1 789 219 | 1 789 680 | 100 | 11 198.2 | 10.63 |
PpSAUR17 | Prupe.3G035000 | 2 557 789 | 2 558 556 | 94 | 10 323.8 | 7.51 |
PpSAUR18 | Prupe.3G035100 | 2 561 778 | 2 562 092 | 101 | 11 069.5 | 5.82 |
PpSAUR19 | Prupe.3G177500 | 19 444 270 | 19 444 578 | 105 | 11 878.7 | 8.49 |
PpSAUR20 | Prupe.4G136800 | 7 593 725 | 7 595 443 | 101 | 11 114.6 | 6.51 |
基因名称 Gene name | 基因登录号 Gene ID | 染色体位置Chromosome location | 蛋白质Protein | |||
起始位点/bp Start position | 终止位点/bp End position | 长度/aa Length | 分子量/D MW | 理论等点 pI | ||
PpSAUR21 | Prupe.4G140200 | 7 830 216 | 7 831 392 | 100 | 11 186.7 | 9.54 |
PpSAUR22 | Prupe.5G076200 | 9 038 680 | 9 039 809 | 101 | 11 014.4 | 5.64 |
PpSAUR23 | Prupe.5G147100 | 13 508 896 | 13 509 615 | 97 | 11 199.2 | 10.16 |
PpSAUR24 | Prupe.6G108300 | 7 731 068 | 7 732 074 | 101 | 11 239.8 | 8.46 |
PpSAUR25 | Prupe.6G108400 | 7 734 034 | 7 735 462 | 95 | 10 557.3 | 8.50 |
PpSAUR26 | Prupe.6G108500 | 7 738 492 | 7 739 254 | 92 | 10 194.6 | 5.64 |
PpSAUR27 | Prupe.6G232300 | 23 489 981 | 23 490 760 | 95 | 10 594.2 | 9.09 |
PpSAUR28 | Prupe.6G234900 | 23 623 506 | 23 623 883 | 94 | 10 125.5 | 5.02 |
PpSAUR29 | Prupe.7G048400 | 8 547 207 | 8 549 249 | 94 | 10 154.6 | 6.51 |
PpSAUR30 | Prupe.7G048500 | 8 552 726 | 8 553 184 | 91 | 9 912.4 | 6.78 |
PpSAUR31 | Prupe.7G048600 | 8 555 516 | 8 555 977 | 102 | 11 231.0 | 8.50 |
PpSAUR32 | Prupe.7G104000 | 13 346 482 | 13 347 250 | 102 | 11 251.0 | 9.22 |
PpSAUR33 | Prupe.7G120400 | 14 389 996 | 14 390 765 | 99 | 11 347.4 | 10.09 |
PpSAUR34 | Prupe.7G167000 | 17 051 802 | 17 052 822 | 95 | 10 491.2 | 8.51 |
PpSAUR35 | Prupe.7G192600 | 18 371 150 | 18 371 953 | 101 | 11 152.6 | 5.82 |
PpSAUR36 | Prupe.7G192900 | 18 381 958 | 18 384 145 | 102 | 11 092.6 | 7.57 |
PpSAUR37 | Prupe.8G063100 | 9 167 730 | 9 168 155 | 83 | 9 202.8 | 10.60 |
PpSAUR38 | Prupe.8G072300 | 10 611 481 | 10 611 777 | 110 | 12 308.0 | 5.68 |
PpSAUR39 | Prupe.8G072400 | 10 619 587 | 10 619 877 | 98 | 11 109.5 | 5.83 |
PpSAUR40 | Prupe.8G078300 | 11 247 032 | 11 247 412 | 92 | 10 118.5 | 8.73 |
PpSAUR41 | Prupe.8G078600 | 11 268 588 | 11 269 189 | 94 | 10 373.9 | 6.51 |
PpSAUR42 | Prupe.8G078700 | 11 272 704 | 11 273 458 | 100 | 10 956.4 | 5.02 |
PpSAUR43 | Prupe.8G078800 | 11 274 113 | 11 275 033 | 101 | 11 084.6 | 5.82 |
PpSAUR44 | Prupe.8G078900 | 11 275 613 | 11 276 700 | 94 | 10 170.6 | 6.50 |
PpSAUR45 | Prupe.8G079000 | 11 277 974 | 11 278 668 | 101 | 11 105.7 | 8.21 |
PpSAUR46 | Prupe.8G079100 | 11 279 560 | 11 280 268 | 75 | 8 307.4 | 9.72 |
PpSAUR47 | Prupe.8G079200 | 11 283 706 | 11 284 527 | 100 | 11 156.7 | 6.96 |
PpSAUR48 | Prupe.8G079300 | 11 285 739 | 11 286 129 | 142 | 16 708.7 | 11.69 |
PpSAUR49 | Prupe.8G079400 | 11 317 660 | 11 318 049 | 91 | 10 108.4 | 5.65 |
PpSAUR50 | Prupe.8G079500 | 11 319 064 | 11 319 345 | 130 | 14 432.6 | 9.50 |
PpSAUR51 | Prupe.8G079600 | 11 320 009 | 11 320 901 | 83 | 9 461.8 | 4.25 |
PpSAUR52 | Prupe.8G079700 | 11 321 405 | 11 322 307 | 143 | 16 113.0 | 6.78 |
PpSAUR53 | Prupe.8G079900 | 11 334 888 | 11 335 121 | 126 | 14 374.5 | 8.41 |
PpSAUR54 | Prupe.8G080000 | 11 347 844 | 11 348 229 | 141 | 15 995.3 | 8.26 |
PpSAUR55 | Prupe.8G080100 | 11 365 948 | 11 367 159 | 139 | 15 191.0 | 6.26 |
PpSAUR56 | Prupe.8G080200 | 11 368 586 | 11 368 888 | 129 | 14 373.4 | 6.92 |
PpSAUR57 | Prupe.8G080300 | 11 401 448 | 11 402 420 | 140 | 15 993.1 | 9.61 |
PpSAUR58 | Prupe.8G080400 | 11 404 019 | 11 404 243 | 136 | 15 427.5 | 8.71 |
PpSAUR59 | Prupe.8G080500 | 11 439 488 | 11 439 769 | 176 | 19 727.6 | 9.92 |
PpSAUR60 | Prupe.8G080600 | 11 445 368 | 11 447 102 | 167 | 19 436.6 | 11.24 |
PpSAUR61 | Prupe.8G080700 | 11 447 103 | 11 448 991 | 153 | 17 140.7 | 8.96 |
PpSAUR62 | Prupe.8G080800 | 11 463 264 | 11 463 545 | 154 | 17 115.6 | 10.24 |
PpSAUR63 | Prupe.8G081000 | 11 469 135 | 11 470 199 | 133 | 14 817.0 | 8.35 |
PpSAUR64 | Prupe.8G081100 | 11 471 700 | 11 473 503 | 208 | 23 314.0 | 10.40 |
PpSAUR65 | Prupe.8G081200 | 11 473 808 | 11 474 113 | 107 | 12 082.8 | 8.71 |
PpSAUR66 | Prupe.8G081300 | 11 475 669 | 11 476 574 | 193 | 21 340.7 | 6.79 |
PpSAUR67 | Prupe.8G081400 | 11 478 089 | 11 478 731 | 126 | 14 033.7 | 5.19 |
PpSAUR68 | Prupe.8G081500 | 11 480 644 | 11 480 943 | 130 | 14 265.3 | 7.44 |
PpSAUR69 | Prupe.8G081600 | 11 482 304 | 11 483 354 | 154 | 17 300.3 | 10.11 |
PpSAUR70 | Prupe.8G081700 | 11 483 436 | 11 485 084 | 118 | 13 160.0 | 6.51 |
PpSAUR71 | Prupe.8G081800 | 11 485 961 | 11 486 206 | 146 | 16 597.0 | 8.50 |
PpSAUR72 | Prupe.8G081900 | 11 487 552 | 11 488 319 | 189 | 21 234.2 | 9.62 |
PpSAUR73 | Prupe.8G082100 | 11 514 115 | 11 514 913 | 103 | 11 608.7 | 10.19 |
PpSAUR74 | Prupe.8G082200 | 11 528 634 | 11 529 256 | 105 | 11 938.9 | 9.86 |
PpSAUR75 | Prupe.8G157700 | 16 792 611 | 16 792 886 | 151 | 17 033.3 | 10.23 |
PpSAUR76 | Prupe.8G157800 | 16 794 545 | 16 795 015 | 106 | 12 089.9 | 8.24 |
PpSAUR77 | Prupe.8G157900 | 16 797 713 | 16 798 249 | 141 | 15 509.0 | 8.33 |
PpSAUR78 | Prupe.8G158000 | 16 799 338 | 16 800 140 | 167 | 18 583.6 | 10.43 |
PpSAUR79 | Prupe.8G158100 | 16 802 092 | 16 802 385 | 165 | 18 522.6 | 9.92 |
PpSAUR80 | Prupe.8G158200 | 16 803 867 | 16 804 613 | 189 | 21 198.9 | 8.38 |
表2 桃树PpSAUR基因家族成员的基本信息
Table 2 Basic information of PpSAUR gene family members in peach
基因名称 Gene name | 基因登录号 Gene ID | 染色体位置Chromosome location | 蛋白质Protein | |||
---|---|---|---|---|---|---|
起始位点/bp Start position | 终止位点/bp End position | 长度/aa Length | 分子量/D MW | 理论等点 pI | ||
PpSAUR1 | Prupe.1G000200 | 42 266 | 44 493 | 173 | 19 380.4 | 11.05 |
PpSAUR2 | Prupe.1G067400 | 4 810 025 | 4 811 236 | 126 | 14 192.5 | 8.63 |
PpSAUR3 | Prupe.1G221300 | 23 585 167 | 23 586 506 | 127 | 14 725.8 | 8.23 |
PpSAUR4 | Prupe.1G367800 | 33 658 384 | 33 660 037 | 126 | 14 253.5 | 9.14 |
PpSAUR5 | Prupe.1G368100 | 33 683 348 | 33 685 088 | 131 | 15 426.6 | 7.38 |
PpSAUR6 | Prupe.1G455700 | 38 251 559 | 38 252 129 | 127 | 14 616.0 | 8.15 |
PpSAUR7 | Prupe.2G131400 | 18 964 628 | 18 965 376 | 122 | 13 712.8 | 9.44 |
PpSAUR8 | Prupe.2G131500 | 18 966 838 | 18 967 203 | 98 | 11 226.7 | 7.22 |
PpSAUR9 | Prupe.2G131600 | 18 972 827 | 18 973 204 | 91 | 9 998.4 | 7.51 |
PpSAUR10 | Prupe.2G140600 | 19 729 097 | 19 730 272 | 105 | 11 631.2 | 7.50 |
PpSAUR11 | Prupe.2G194600 | 23 275 229 | 23 276 493 | 82 | 9 069.3 | 5.02 |
PpSAUR12 | Prupe.2G211600 | 24 387 357 | 24 388 174 | 84 | 9 269.6 | 9.19 |
PpSAUR13 | Prupe.2G317100 | 29 700 658 | 29 701 286 | 151 | 16 864.3 | 9.12 |
PpSAUR14 | Prupe.3G023900 | 1 785 030 | 1 786 044 | 78 | 8 619.9 | 8.98 |
PpSAUR15 | Prupe.3G024000 | 1 787 115 | 1 787 919 | 102 | 11 216.9 | 8.21 |
PpSAUR16 | Prupe.3G024100 | 1 789 219 | 1 789 680 | 100 | 11 198.2 | 10.63 |
PpSAUR17 | Prupe.3G035000 | 2 557 789 | 2 558 556 | 94 | 10 323.8 | 7.51 |
PpSAUR18 | Prupe.3G035100 | 2 561 778 | 2 562 092 | 101 | 11 069.5 | 5.82 |
PpSAUR19 | Prupe.3G177500 | 19 444 270 | 19 444 578 | 105 | 11 878.7 | 8.49 |
PpSAUR20 | Prupe.4G136800 | 7 593 725 | 7 595 443 | 101 | 11 114.6 | 6.51 |
基因名称 Gene name | 基因登录号 Gene ID | 染色体位置Chromosome location | 蛋白质Protein | |||
起始位点/bp Start position | 终止位点/bp End position | 长度/aa Length | 分子量/D MW | 理论等点 pI | ||
PpSAUR21 | Prupe.4G140200 | 7 830 216 | 7 831 392 | 100 | 11 186.7 | 9.54 |
PpSAUR22 | Prupe.5G076200 | 9 038 680 | 9 039 809 | 101 | 11 014.4 | 5.64 |
PpSAUR23 | Prupe.5G147100 | 13 508 896 | 13 509 615 | 97 | 11 199.2 | 10.16 |
PpSAUR24 | Prupe.6G108300 | 7 731 068 | 7 732 074 | 101 | 11 239.8 | 8.46 |
PpSAUR25 | Prupe.6G108400 | 7 734 034 | 7 735 462 | 95 | 10 557.3 | 8.50 |
PpSAUR26 | Prupe.6G108500 | 7 738 492 | 7 739 254 | 92 | 10 194.6 | 5.64 |
PpSAUR27 | Prupe.6G232300 | 23 489 981 | 23 490 760 | 95 | 10 594.2 | 9.09 |
PpSAUR28 | Prupe.6G234900 | 23 623 506 | 23 623 883 | 94 | 10 125.5 | 5.02 |
PpSAUR29 | Prupe.7G048400 | 8 547 207 | 8 549 249 | 94 | 10 154.6 | 6.51 |
PpSAUR30 | Prupe.7G048500 | 8 552 726 | 8 553 184 | 91 | 9 912.4 | 6.78 |
PpSAUR31 | Prupe.7G048600 | 8 555 516 | 8 555 977 | 102 | 11 231.0 | 8.50 |
PpSAUR32 | Prupe.7G104000 | 13 346 482 | 13 347 250 | 102 | 11 251.0 | 9.22 |
PpSAUR33 | Prupe.7G120400 | 14 389 996 | 14 390 765 | 99 | 11 347.4 | 10.09 |
PpSAUR34 | Prupe.7G167000 | 17 051 802 | 17 052 822 | 95 | 10 491.2 | 8.51 |
PpSAUR35 | Prupe.7G192600 | 18 371 150 | 18 371 953 | 101 | 11 152.6 | 5.82 |
PpSAUR36 | Prupe.7G192900 | 18 381 958 | 18 384 145 | 102 | 11 092.6 | 7.57 |
PpSAUR37 | Prupe.8G063100 | 9 167 730 | 9 168 155 | 83 | 9 202.8 | 10.60 |
PpSAUR38 | Prupe.8G072300 | 10 611 481 | 10 611 777 | 110 | 12 308.0 | 5.68 |
PpSAUR39 | Prupe.8G072400 | 10 619 587 | 10 619 877 | 98 | 11 109.5 | 5.83 |
PpSAUR40 | Prupe.8G078300 | 11 247 032 | 11 247 412 | 92 | 10 118.5 | 8.73 |
PpSAUR41 | Prupe.8G078600 | 11 268 588 | 11 269 189 | 94 | 10 373.9 | 6.51 |
PpSAUR42 | Prupe.8G078700 | 11 272 704 | 11 273 458 | 100 | 10 956.4 | 5.02 |
PpSAUR43 | Prupe.8G078800 | 11 274 113 | 11 275 033 | 101 | 11 084.6 | 5.82 |
PpSAUR44 | Prupe.8G078900 | 11 275 613 | 11 276 700 | 94 | 10 170.6 | 6.50 |
PpSAUR45 | Prupe.8G079000 | 11 277 974 | 11 278 668 | 101 | 11 105.7 | 8.21 |
PpSAUR46 | Prupe.8G079100 | 11 279 560 | 11 280 268 | 75 | 8 307.4 | 9.72 |
PpSAUR47 | Prupe.8G079200 | 11 283 706 | 11 284 527 | 100 | 11 156.7 | 6.96 |
PpSAUR48 | Prupe.8G079300 | 11 285 739 | 11 286 129 | 142 | 16 708.7 | 11.69 |
PpSAUR49 | Prupe.8G079400 | 11 317 660 | 11 318 049 | 91 | 10 108.4 | 5.65 |
PpSAUR50 | Prupe.8G079500 | 11 319 064 | 11 319 345 | 130 | 14 432.6 | 9.50 |
PpSAUR51 | Prupe.8G079600 | 11 320 009 | 11 320 901 | 83 | 9 461.8 | 4.25 |
PpSAUR52 | Prupe.8G079700 | 11 321 405 | 11 322 307 | 143 | 16 113.0 | 6.78 |
PpSAUR53 | Prupe.8G079900 | 11 334 888 | 11 335 121 | 126 | 14 374.5 | 8.41 |
PpSAUR54 | Prupe.8G080000 | 11 347 844 | 11 348 229 | 141 | 15 995.3 | 8.26 |
PpSAUR55 | Prupe.8G080100 | 11 365 948 | 11 367 159 | 139 | 15 191.0 | 6.26 |
PpSAUR56 | Prupe.8G080200 | 11 368 586 | 11 368 888 | 129 | 14 373.4 | 6.92 |
PpSAUR57 | Prupe.8G080300 | 11 401 448 | 11 402 420 | 140 | 15 993.1 | 9.61 |
PpSAUR58 | Prupe.8G080400 | 11 404 019 | 11 404 243 | 136 | 15 427.5 | 8.71 |
PpSAUR59 | Prupe.8G080500 | 11 439 488 | 11 439 769 | 176 | 19 727.6 | 9.92 |
PpSAUR60 | Prupe.8G080600 | 11 445 368 | 11 447 102 | 167 | 19 436.6 | 11.24 |
PpSAUR61 | Prupe.8G080700 | 11 447 103 | 11 448 991 | 153 | 17 140.7 | 8.96 |
PpSAUR62 | Prupe.8G080800 | 11 463 264 | 11 463 545 | 154 | 17 115.6 | 10.24 |
PpSAUR63 | Prupe.8G081000 | 11 469 135 | 11 470 199 | 133 | 14 817.0 | 8.35 |
PpSAUR64 | Prupe.8G081100 | 11 471 700 | 11 473 503 | 208 | 23 314.0 | 10.40 |
PpSAUR65 | Prupe.8G081200 | 11 473 808 | 11 474 113 | 107 | 12 082.8 | 8.71 |
PpSAUR66 | Prupe.8G081300 | 11 475 669 | 11 476 574 | 193 | 21 340.7 | 6.79 |
PpSAUR67 | Prupe.8G081400 | 11 478 089 | 11 478 731 | 126 | 14 033.7 | 5.19 |
PpSAUR68 | Prupe.8G081500 | 11 480 644 | 11 480 943 | 130 | 14 265.3 | 7.44 |
PpSAUR69 | Prupe.8G081600 | 11 482 304 | 11 483 354 | 154 | 17 300.3 | 10.11 |
PpSAUR70 | Prupe.8G081700 | 11 483 436 | 11 485 084 | 118 | 13 160.0 | 6.51 |
PpSAUR71 | Prupe.8G081800 | 11 485 961 | 11 486 206 | 146 | 16 597.0 | 8.50 |
PpSAUR72 | Prupe.8G081900 | 11 487 552 | 11 488 319 | 189 | 21 234.2 | 9.62 |
PpSAUR73 | Prupe.8G082100 | 11 514 115 | 11 514 913 | 103 | 11 608.7 | 10.19 |
PpSAUR74 | Prupe.8G082200 | 11 528 634 | 11 529 256 | 105 | 11 938.9 | 9.86 |
PpSAUR75 | Prupe.8G157700 | 16 792 611 | 16 792 886 | 151 | 17 033.3 | 10.23 |
PpSAUR76 | Prupe.8G157800 | 16 794 545 | 16 795 015 | 106 | 12 089.9 | 8.24 |
PpSAUR77 | Prupe.8G157900 | 16 797 713 | 16 798 249 | 141 | 15 509.0 | 8.33 |
PpSAUR78 | Prupe.8G158000 | 16 799 338 | 16 800 140 | 167 | 18 583.6 | 10.43 |
PpSAUR79 | Prupe.8G158100 | 16 802 092 | 16 802 385 | 165 | 18 522.6 | 9.92 |
PpSAUR80 | Prupe.8G158200 | 16 803 867 | 16 804 613 | 189 | 21 198.9 | 8.38 |
图2 桃PpSAUR蛋白的系统发育关系(a),基因结构(b)和保守基序分布(c)
Fig. 2 Phylogenetic relationship(a),gene structure(b)and distribution of conserved motifs(c)of PpSAUR proteins in peach
图5 在MS培养基生长20 d的拟南芥植株的鲜质量、干质量和含水量分析
Fig. 5 Analysis of fresh weight,dry weight and water content of Arabidopsis plants growing on MS medium for 20 days n = 20.
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