园艺学报 ›› 2023, Vol. 50 ›› Issue (2): 359-370.doi: 10.16420/j.issn.0513-353x.2021-0987

• 研究论文 • 上一篇    下一篇


任菲, 卢苗苗, 刘吉祥, 陈信立, 刘道凤, 眭顺照, 马婧*()   

  1. 西南大学园艺园林学院,重庆市花卉工程技术研究中心,南方山地园艺学教育部重点实验室,重庆 400715
  • 收稿日期:2022-09-03 修回日期:2022-10-27 出版日期:2023-02-25 发布日期:2023-03-06
  • 通讯作者: *(
  • 基金资助:

Expression and Adversity Resistance Analysis of a Late Embryogenesis Abundant Protein Gene CpLEA from Chimonanthus praecox

REN Fei, LU Miaomiao, LIU Jixiang, CHEN Xinli, LIU Daofeng, SUI Shunzhao, MA Jing*()   

  1. Chongqing Engineering Research Center for Floriculture,Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,College of Horticulture and Landscape,Southwest University,Chongqing 400715,China
  • Received:2022-09-03 Revised:2022-10-27 Online:2023-02-25 Published:2023-03-06
  • Contact: *(


将蜡梅(Chimonanthus praecox)第3组LEA蛋白基因CpLEA在大肠杆菌中进行诱导表达,结果发现CpLEA重组蛋白在体外低温胁迫下能够保护乳酸脱氢酶的活性,且转CpLEA大肠杆菌的抗寒性有所提高;该基因在拟南芥中超表达,拟南芥的抗寒性和抗旱性均有所提高,且抗性的提高程度与基因过表达的积累量正相关,证实该基因能够参与植物的抗逆过程,在低温和渗透胁迫响应中发挥一定作用。通过qRT-PCR进一步分析花蕾期和露瓣期蜡梅瓶插花在低温(4 ℃)胁迫后CpLEA的表达特性,结果表明:与对照(22 ℃)相比,花蕾期CpLEA表达量在低温胁迫后逐渐升高,48 h达到峰值,约为未处理表达量的28倍,随后表达量下降,但仍然高于对照;露瓣期时该基因表达量随低温处理时间的延长逐渐升高,72 h后达到最高,约为未处理表达量的9倍。室温下蜡梅瓶插花中CpLEA的表达量基本恒定,而低温处理则可诱导该基因在蜡梅花发育早期表达量有不同程度的提高。

关键词: 蜡梅, LEA, 原核表达, 转基因, 低温胁迫


To characterize the function of CpLEA,a group three LEA protein gene of Chimonanthus praecox(wintersweet),CpLEA is transformed into Escherichia coli and Arabidopsis thaliana. Compared with the control strain,transgenic E. coli showed higher tolerance to low-temperature. In vitro,purified CpLEA protein could protect lactate dehydrogenase(LDH)from inactivation. Overexpression of the CpLEA gene in Arabidopsis also improved the resistance of transgenic Arabidopsis to cold and drought stresses and the degree of improved resistance was positively correlated with the accumulation of gene overexpression. The expression characteristics of CpLEA gene were further analyzed by qRT-PCR in wintersweet cutflower branch during flower-bud and display-petal stage respectively under 4 ℃ low-temperature treatment. CpLEA gene expression was found to gradually increase after low-temperature treatment in cutflower branch at flower-bud stage,reaching a peak at 48 h of treatment,which was about 28 times higher than expression of the untreated group,and then the expression decreased,but was still higher than the untreated group. The expression of CpLEA gradually increased with the extension of low-temperature treatment time in cutflower branch at display-petal stage,then reached the highest level at 72 h,which was nine times as much as the untreated group. The expression of CpLEA in cutflowers remained basically constant at room temperature,while the low-temperature treatment induced a different degree of expression increase of CpLEA in the early stage of flower development. These results indicated that the CpLEA would play a protective role in plant cells under low-temperature stress,especially in early stage of flower development.

Key words: Chimonanthus praecox, LEA, prokaryotic expression, transgenic, low-temperature stress