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园艺学报 ›› 2023, Vol. 50 ›› Issue (1): 161-169.doi: 10.16420/j.issn.0513-353x.2021-0756

• 研究报告 • 上一篇    下一篇

蚕豆萎蔫病毒2号青海辣椒分离物的鉴定与全基因组序列克隆

罗海林1, 袁雷1, 翁华1, 闫佳会1,*(), 郭青云1,*(), 王文清2, 马新明3   

  1. 1青海大学农林科学院,青海省农业有害生物综合治理重点实验室,农业农村部西宁作物有害生物科学观测实验站,西宁 810016
    2大通回族土族自治县蔬菜技术服务中心,西宁 810016
    3循化撒拉族自治县农业农村和科技局,青海海东 810600
  • 收稿日期:2022-05-12 修回日期:2022-08-13 出版日期:2023-01-25 发布日期:2023-01-18
  • 通讯作者: *(E-mail:15209787170@163.comguoqingyunqh@163.com
  • 基金资助:
    青海省科技厅国际合作项目(2022-HZ-806);青海省农业有害生物综合治理重点实验室资助项目(2022-ZJ-Y10);青海省科技厅重点与成果转化项目(2019-NK-114)

Identification and Analysis of Complete Genomic Sequence of Broad Bean Wilt Virus 2 Pepper Isolate in Qinghai Province

LUO Hailin1, YUAN Lei1, WENG Hua1, YAN Jiahui1,*(), GUO Qingyun1,*(), WANG Wenqing2, MA Xinming3   

  1. 1Key Laboratory of Agricultural Integrated Pest Management,Scientific Observing and Experimental Station of Crop Pest in Xining,Ministry of Agriculture and Rural Affairs,Academy of Agriculture and Forestry Sciences,Qinghai University,Xining 810016,China
    2Vegetable Technical Service Center of Datong County,Xining 810016,China
    3Bureau of Agriculture and Rural & Science and Technology Affairs of Xunhua County,Haidong,Qinghai 810600,China
  • Received:2022-05-12 Revised:2022-08-13 Online:2023-01-25 Published:2023-01-18
  • Contact: *(E-mail:15209787170@163.comguoqingyunqh@163.com

摘要:

对青海省循化县的辣椒病毒病进行调查,采集到部分叶片表现萎蔫和皱缩症状的样品,该症状与蚕豆萎蔫病毒2(BBWV2)引起的症状相似。为准确鉴定是否为与该病害相关的病毒,将采集的20个辣椒样品用特异性引物进行RT-PCR检测,其中13个样品检测结果为阳性。在20份循化县辣椒样品中,有13份样品被确认感染了蚕豆萎蔫病毒2(BBWV2)。基于核苷酸序列的系统发育分析表明,检测到的病毒与BBWV2聚类在一起,与其他地区或国家分离物的序列一致性较高,与蚕豆萎蔫病毒1(BBWV1)和野芝麻轻型花叶病毒(LMMV)分离物的序列一致性相对较低。结果表明该辣椒样品感染的病毒是BBWV2。

关键词: 辣椒, 蚕豆萎蔫病毒2, 全基因组

Abstract:

An investigation on pepper virus was conducted. Samples collected in Xunhua County,Qinghai Province shown symptoms of plant wilt and leaf shrinkage,which were very similar to the symptoms induced by broad bean wilt virus 2(BBWV2). To identify the possible virus associated with the disease,20 samples were collected and detected with universal primer and specific primer using RT-PCR

and 13 samples shown positive results when using a pair of degenerate primers for BBWV2. The results showed that 13 of 20 pepper samples in Xunhua County were confirmed to be infected with broad bean wilting virus 2. Further phylogenetic analysis based on the nucleotide sequence also suggested that the virus detected was clustered with BBWV2 and shared the highest sequence identities with the isolates from abroad,and relatively lower identities with the isolates of broad bean wilt virus 1(BBWV1)or Lamium mild mosaic virus(LMMV). All these results showed that the virus infected pepper is an isolate of BBWV2.

Key words: pepper, broad bean wilt virus, whole genome

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