Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (2): 352-364.doi: 10.16420/j.issn.0513-353x.2020-1013

• Research Papers • Previous Articles     Next Articles

Genome-wide Identification and Expression Analyses of Long-chain Acyl-CoA Synthetases Under Abiotic Stresses in Helianthus annuus

ZHOU Zhiming, YANG Jiabao, ZHANG Cheng, ZENG Linglu, MENG Wanqiu, SUN Li()   

  1. College of Life Sciences,Shihezi University,Shihezi,Xinjiang 832003,China
  • Received:2021-07-01 Revised:2021-12-23 Online:2022-02-25 Published:2022-02-28
  • Contact: SUN Li


Based on bioinformatics analyses,13 long-chain acyl-CoA synthetases(LACSs)putative genes from LACS gene family were identified from the genome of sunflower(Helianthus annuus)and mapped to eight chromosomes. The phylogenetic analysis showed that HaLACSs clustered into six subgroups and HaLACS proteins within each subgroup share the similar structures and conserved motifs. The main factors for HaLACS family amplification in sunflower were segmental duplication. Bioinformatic analysis of HaLACS genes promoter regions showed multiple cis-elements related to environmental stresses and plant hormone response. Tissue expression profile analysis showed that the HaLACS genes exhibited differential expression profiles in various tissues. The comparison of gene expression profiles between two sunflower cultivars(high/low linoleic acid)found that HaLACSs showed differential expression at different seed development stages. The qRT-PCR analysis showed that seven HaLACS up-regulated significantly in sunflower stem treated with 200 mmol · L-1 NaCl for 24 h compared with control. After three hours treatment with 100 μmol · L-1 ABA,eight HaLACS synchronized especially in roots,and most HaLACS colud be induced by drought stress(15% PEG 6000). The above results indicate that the HaLACS play essential roles in dealing with different abiotic stresses and lipid biosynthesis.

Key words: sunflower, LACS gene family, abiotic stress, gene expression

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