Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (11): 2489-2501.doi: 10.16420/j.issn.0513-353x.2021-0763

• New Technology and New Methods • Previous Articles     Next Articles

Selection and Validation of Reference Genes for RT-qPCR in Phalaenopsis- type Dendrobium Hybrid

HOU Tianze1, YI Shuangshuang2,3, ZHANG Zhiqun2,3, WANG Jian1,*(), LI Chonghui2,3,*   

  1. 1College of Horticulture,Hainan University,Haikou 570208,China
    2Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agriculture and Rural affairs,Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China
    3The Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization,Hainan Province,Danzhou,Hainan 571737,China
  • Received:2021-10-15 Revised:2022-04-30 Online:2022-11-25 Published:2022-11-25
  • Contact: WANG Jian,LI Chonghui


In order to screen reference genes stably expressing in Phalaenopsis-type Dendrobium hybrid for analysis of real-time fluorescent quantitative PCR,the whole floral buds,including the sepals,petals and labellums,from various cultivars and the floral bud at different development stages of dendrobium were used as materials. The expression stability of seven candidate gene was calculated by three commonly used software(geNorm,NormFinder and BestKeeper),comprehensive stability rankings were merged by RefFinder and the selected reference genes were verified. The results suggested that the expression stability of each reference gene in dendrobium differed greatly. In floral buds of different cultivars at the same developmental stage,β-actinTUA,and GADPH were the most stable reference genes. The expression of β-actinTUA,and CYP were the most stable during the development of the floral buds. For different tissues of floral organ,β-actinTUA,and GADPH were the most stable reference genes in the sepals,β-actinCYP,and PGK were in the petals and CYPTUA,and PGK were in the labellum.

Key words: Phalaenopsis-type Dendrobium hybrid, floral bud, developmental stage, tissues, RT-qPCR, reference gene, gene expression

CLC Number: