Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (9): 1945-1956.doi: 10.16420/j.issn.0513-353x.2021-0794

• Research Papers • Previous Articles     Next Articles

Cloning and Expression Analysis of RcDREB2A Gene in Rosa chinensis‘Old Blush’

JIA Xin1, ZENG Zhen1, CHEN Yue1, FENG Hui2, LÜ Yingmin1,*(), ZHAO Shiwei2,*()   

  1. 1Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding,Beijing Laboratory of Urban and Rural Ecological Environment,National Engineering Research Center for Floriculture,School of Landscape and Architecture,Beijing Forestry University,Beijing 100083,China
    2Beijing Key Laboratory of Greening Plant Breeding,Beijing Institute of Landscape Architecture,Beijing 100102,China
  • Received:2022-04-07 Revised:2022-07-01 Online:2022-09-25 Published:2022-10-08
  • Contact: LÜ Yingmin,ZHAO Shiwei;


Cutting of Rosa chinensis‘Old Blush’were used as the material to clone the AP2/ERF transcription factor RCDREB2A gene based on transcriptome under drought stress. The RcDREB2A gene contained a 1 155 bp open reading frame(ORF),encoding 384 amino acids and contained a conserved AP2 domain between 75-142 amino acids. Phylogenetic analysis showed that RcDREB2A has the highest affinity with Fragaria veaca FvDREB2A(XP_004307690.1). RcDREB2A protein has a predicted molecular weight of 5 737,a theoretical isoelectric point of 4.75. RcDREB2A protein has no transmembrane domain and no signal peptide. RcDREB2A protein was localized in the nucleus and showed no transcriptional activation activity. RcDREB2A was highly expressed in leaves,and it could be up-regulated by ABA,drought,low temperature and salt stresses. The above results indicated that RcDREB2A may participate in a variety of abiotic stress responses of rose and plays a crucial role in resisting abiotic stresses.

Key words: rose, RcDREB2A, abiotic stress, transcription factor

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