Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (1): 100-116.doi: 10.16420/j.issn.0513-353x.2020-0850

• Research Papers • Previous Articles     Next Articles

Genome-wide Identification and Expression Analysis of CsTIFY Transcription Factor Family Under Abiotic Stress and Hormone Treatments in Camellia sinensis

XIE Siyi1,3, ZHOU Chengzhe1,2,3, ZHU Chen1,2,3, ZHAN Dongmei1,3, CHEN Lan1,3, WU Zuchun1,3, LAI Zhongxiong1,2, GUO Yuqiong1,3,*()   

  1. 1College of Horticulture,Tea Industry Research Institute,Fujian Agriculture and Forestry University,Fuzhou 350002,China
    2Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China
    3Key Laboratory of Tea Science of Fujian Province,Fujian Agriculture and Forestry University,Fuzhou 350002,China
  • Received:2021-04-23 Revised:2021-07-26 Online:2022-01-25 Published:2022-01-24
  • Contact: GUO Yuqiong


TIFY transcription factors are unique transcription factors of terrestrial plants. They are divided into four sub-families TIFY,PPD,JAZ,ZML according to structural domain characteristics,and regulate the growth and development of plants. In this paper,the TIFY transcription factor of tea plant is the research object. A total of 22 members of the CsTIFY transcription factor family have been identified from the tea plant genome database,all of which contain the TIFY domain. Gene structure analysis found that most of the CsTIFY family members have exons between five and 12,and the same subfamily contains similar numbers of motifs and exons. The results of the phylogenetic tree showed that the 122 genes of six species were divided into four groups,among which tea trees were more closely related to grapes and poplars. Analysis of promoter cis-acting elements found that the CsTIFY family contains a large number of phytohormones and abiotic stress response elements such as SA,ABA,low temperature and drought. The results of subcellular localization experiments showed that CsTIFY1,CsJAZ1 and CsJAZ5 were localized in the nucleus. The tissue expression analysis of different tea cultivars showed that most CsTIFY gene family members had higher relative expression levels in mature leaves,roots and fruits. In addition,the expression of most CsJAZ genes was increased and the expression of CsZML was decreased under the treatments of exogenous hormones ABA,SA and MeJA. The expression of CsZML1 was significantly increased under both low temperature and drought treatments.

Key words: Camellia sinensis, TIFY transcription factor, genome-wide identification, ABA, SA, MeJA, abiotic stress, subcellular localization

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