Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (2): 237-249.doi: 10.16420/j.issn.0513-353x.2021-1117

• Research Papers •     Next Articles

Effects of Abscisic Acid on Monoterpene Synthesis in‘Jingxiangyu’Grape Berries

WANG Xiaochen1,2,3, NIE Ziye1,2,3, LIU Xianju1,2, DUAN Wei1,2, FAN Peige1,2,*(), LIANG Zhenchang1,2,*()   

  1. 1. Beijing Key Laboratory of Grape Science and Enology,Key Laboratory of Plant Resources Institute,the LIA INNOGRAPE International Associated Laboratory,Institute of Botany,the Chinese Academy of Sciences,Beijing 100089,China
    2. China National Botanical Garden,Beijing 100093,China
    3. University of Chinese Academy of Sciences,Beijing 101400,China
  • Received:2022-10-14 Revised:2022-12-05 Online:2023-02-25 Published:2023-03-06
  • Contact: *(


In order to explore the effects of abscisic acid(ABA)on monoterpene synthesis in grape berries,this study used table grape species‘Jingxiangyu'with muscat flavor as experimental material,and soaked the clusters with 300 mg · L-1 ABA twice at one week before and during veraison. The contents of free monoterpenoids at 7,14,21,28 and 35 days after first treatment were analyzed qualitatively and quantitatively by GC-MS. The results showed that 28 kinds of monoterpenes were detected in berries,most of which accumulated during ripening. The total contents of free monoterpenoids in treated berries at maturity was 45.20% higher than that in the control,and the substance that changed most strongly was linalool. To investigate how ABA regulate monoterpenes synthesis,quantitative real-time PCR was used. The results indicated that the expression of ABA synthesis genes(VvNCED1 and VvGT)was much stronger than that of the control. The relative expression of VvβG1 increased after ABA treatment constantly. The transcriptional levels of ABA receptor VvPYL1 and signal transduction related genes VvPP2C2VvSnRK2.1 and VvABI5 were distinctly higher than the control on 21 and 35 days after treatment respectively. The expression levels of upstream genes about monoterpene synthesis(VvDXS2VvDXS3VvDXRVvGPPS)and monoterpene synthase gene VvPNLinNer was significantly higher. According to the above results,it’s suggested that exogenous ABA enhance ABA signal transduction in berries,which lead to the increase of monoterpene substrate level and facilitate monoterpene synthesis,finally improve the quality of rose flavor grapes.

Key words: grape, muscat flavor, abscisic acid, monoterpenoids, gene expression

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