Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (2): 341-351.doi: 10.16420/j.issn.0513-353x.2020-1041

• Research Papers • Previous Articles     Next Articles

Cytological Observation of Fruit Peduncle Abscission Zone and Preliminary Mapping of Mature Fruit Abscission AL3 gene in Melon

SHENG Yunyan1, YANG Limin1, DAI Dongyang1, ZHANG Jiaxin1, WANG Ling1, WANG Di2, CAI Yi1, TIAN Limei1,*()   

  1. 1College of Horticulture and Landscape Architecture,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China
    2Daqing Branch of Heilongjiang Academy of Agricultural Sciences,Daqing,Heilongjiang 163310,China
  • Received:2021-05-11 Revised:2021-11-03 Online:2022-02-25 Published:2022-02-28
  • Contact: TIAN Limei


The F2,F3,F4 and backcross populations derived from a cross between M2-10 (none-mature fruit abscission,NMFA)and ZT091(mature fruit abscission,MFA)was used to study the cytological observation of abscission zone and gene mapping of one of mature fruit abscission(MFA)gene(AL3).The analysis of genetic inheritance indicated that the MFA was controlled by two genes. Paraffin section and scanning electron microscopy observation of the parent fruit at the maturity stage showed that the cells in the abscission zone of peduncle of ZT091 were larger in size,loosen arrangement and larger spaces between cells,there were significant difference between parental peduncle abscission zone. AL3 was primary mapped into a 0.355 Mb region(from 10 686 821 to 11 042 074 bp)on chromosome 8 with the strategy of SLAF-BSA using 83-F3 family only segregated with AL3. F4 family and CAPs markers were used for narrowing down the mapping region. Finally, AL3 was fined mapped into a 64.7 kb(from 10 774 778 to 10 839 486 bp)region with 10 annotated candidate genes.

Key words: melon, fruit abscission, cyotological observation, gene mapping

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