Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (4): 749-757.doi: 10.16420/j.issn.0513-353x.2021-0292

• Research Papers • Previous Articles     Next Articles

Optimization of Jujube Callus Transformation System and Application of ZjBRC1 in Regulating ZjYUCCA Expression

LI Yamei1,3, MA Fuli1, ZHANG Shanqi1,3, HUANG Jinqiu1, CHEN Mengting1, ZHOU Junyong1,2, SUN Qibao2, SUN Jun1,**()   

  1. 1. College of Horticulture,Anhui Agricultural University,Hefei 230036,China
    2. Horticulture Research Institute,Anhui Academy of Agricultural Science,Hefei 230031,China
    3. State Key Laboratory of Tea Plant Biology and Utilization,Hefei 230036,China
  • Received:2021-11-08 Revised:2022-12-17 Online:2022-04-25 Published:2022-04-24
  • Contact: SUN Jun


The stem segment,leaves,cotyledon and hypocotyls from the same sour jujube(Ziziphus jujuba Mill. var. spinosa)were used as the explants to induce callus. The optimum callus induction was obtained from cotyledon,hypocotyls and leaves in MS media supplemented with 1.0 mg · L-1 2,4- dichlorophenoxyacetic acid(2,4-D)and 0.4 mg · L-1 thidiazuron(TDZ). Furthermore,the callus transformation efficiency was 31.8% in leaves,25.6% in cotyledons,24.5% in hypocotyls and 23.8% in stem segments,respectively,after inoculating callus in the Agrobacterium infection solution with OD600 = 0.6-0.8 for 30 min. In addition,ZjBRC1,which was a homologue of CYC/TB1 TCP transcription factor BRC1 and the key repressor to control shoot branching,were fused to the N terminus of SRDX repressor domain. 35S::GFP and 35S::ZjBRC1-SRDX transgenic calli were obtained via the leaf-induced callus transformation system. qRT-PCR analysis showed that auxin biosynthetic genes ZjYUCCA7/10-3/10-4 were down-regulated while ZjYUCCA2/4 /6 were up-regulated in the 35S::ZjBRC1-SRDX transgenic calli.

Key words: jujube, callus, genetic transformation, ZjBRC1, gene expression

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