Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (2): 365-377.doi: 10.16420/j.issn.0513-353x.2021-0574

• Research Papers • Previous Articles     Next Articles

Transcriptome Analysis Revealed the Formation Mechanism of Floral Color of Lotus‘Dasajin’with Bicolor Petal

DENG Jiao1,2, SU Mengyue1, LIU Xuelian1, OU Kefang3, HU Zhengrong1, YANG Pingfang1,*()   

  1. 1State Key Laboratory of Biocatalysis and Enzyme Engineering,School of Life Sciences,Hubei University,Wuhan 430062,China
    2Research Center of Buckwheat Industry Technology,Guizhou Normal University,Guiyang 550001,China
    3Wuhan Institute of Landscape Architecture,Wuhan 430081,China
  • Received:2021-06-18 Revised:2021-09-06 Online:2022-02-25 Published:2022-02-28
  • Contact: YANG Pingfang


‘Dasajin’lotus with red and white flower was used as the research material,it’s red color of petals was caused by anthocyanins accumulation,which was related to acidification of petal cells. A total of 91 differentially expressed genes(DEGs)were identified between red and white petals by transcriptomics analysis and these DEGs can be divided into molecular function,cellular component and biological process categories base on gene ontology(GO)analysis,among which more genes were related to redox activity,thylakoid and photosynthesis. KEGG metabolic pathway analysis indicated that genes enriched in metabolic pathway,photosynthesis and galactose metabolic branches were more abundant. Fourteen DEGs related to chloroplast and photosynthesis were down regulated in the red petals region,one of which was thylakoid morphogenetic protein 1 gene(THF1),whose low expression level was consistent with structure abnormality of thylakoid. Several transcription factors,including YABBY and bHLH,which were related to petal development,were predicted to bind the promoter of THF1 gene. In addition,several genes involved in biosynthesis of auxin and gibberellin as well as cell development were differentially expressed in red and white petal regions,leading to the formation of concave-like bowl shape of petal. Therefore,it was speculated that anthocyanins selectively accumulated in petal edge region with red color was related to petal development,that is,these genes regulating petal development may also regulate anthocyanins accumulation,which may be involved in thylakoid development,pH regulation and cell development.

Key words: lotus, bicolor flower, flower color, anthocyanin, petal development, transcriptome

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