Identification and Analysis of Self Incompatibility S-RNase in Rose

doi:10.16420/j.issn.0513-353x.2020-0758

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (1): 157-165.doi: 10.16420/j.issn.0513-353x.2020-0758

• Research Notes • Previous Articles Next Articles

Identification and Analysis of Self Incompatibility S-RNase in Rose

LI Maofu¹^,²^,³, YANG Yuan¹^,²^,⁴, WANG Hua¹^,²^,³, FAN Youwei¹^,²^,³, SUN Pei¹^,²^,³, JIN Wanmei¹^,²^,³^,^*()

¹Institute of Forestry and Pomology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100093,China
²Key Laboratory of Biology and Genetic Improvement of Horticultural Crops（North China）,Ministry of Agriculture,Beijing 100093,China
³Beijing Engineering Research Center of Functional Floriculture,Beijing 100093,China
⁴Beijing Engineering Research Center for Deciduous Fruit Trees,Beijing 100093,China

Received:2021-03-30 Revised:2021-09-07 Online:2022-01-25 Published:2022-01-24
Contact: JIN Wanmei E-mail:jwm0809@163.com

Abstract

Abstract:

In Rosa chinensis‘Slater’s Crimson China’and Rosa sp.‘2015-58-20’,the fluorescence microscope observation of pollen tubes in both self-pollinated and cross-pollinated styles indicated that rose is a typical gametophyte self-incompatible plant. The full-length sequence of RcS₁-RNase and RcS₂-RNase cDNA and DNA have been identified in the‘Slater’s Crimson China’. Sequence analysis showed that RcS₁-RNase and RcS₂-RNase have five conserved domains（C1,C2,C3,RC4 and C5）and a hypervariable region. Phylogenetic analysis found that RcS-RNase has high homology with S-RNase proteins in other Rosaceae plants. Tissue specific expression analysis indicated that RcS₁-RNase and RcS₂-RNase genes are solely expressed in styles.

Key words: Rosa, self incompatibility, S-RNase, gene cloning, gene expression

CLC Number:

S685.12

LI Maofu, YANG Yuan, WANG Hua, FAN Youwei, SUN Pei, JIN Wanmei. Identification and Analysis of Self Incompatibility S-RNase in Rose[J]. Acta Horticulturae Sinica, 2022, 49(1): 157-165.

Figures/Tables 8

References 41

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目的 Purpose	基因 Gene	引物序列（5′-3′） Primer sequence
基因克隆 Gene cloning	RcS₁-RNase	F：ATGGGTTCCTATGAATATTTTAAATTTGTGG;R：CTACAGAATATCGATTGGAAAAGTATCTTGAC
基因克隆 Gene cloning	RcS₂-RNase	F：ATGGCACAAAATAATATGGCTTACGTCCTC;R：TCAGTTCGGATATTCTATAAGAGTTGGAGC
RT-PCR	RcS₁-RNase	F：GCGTTTAGTCTGCAATGACGCAGAAGCTC;R：ACTAGTATCAAACGAAATGATTGCTCGCG
	RcS₂-RNase	F：TGTGGTAACAGCTGCAAAGC;R：TCAACCACGTTTTTGCCATA
	Actin	F：GGCTGTTCTCTCTCTGTATGC;R：TTCTGGGCACCTGAATCTC

目的 Purpose	基因 Gene	引物序列（5′-3′） Primer sequence
基因克隆 Gene cloning	RcS₁-RNase	F：ATGGGTTCCTATGAATATTTTAAATTTGTGG;R：CTACAGAATATCGATTGGAAAAGTATCTTGAC
基因克隆 Gene cloning	RcS₂-RNase	F：ATGGCACAAAATAATATGGCTTACGTCCTC;R：TCAGTTCGGATATTCTATAAGAGTTGGAGC
RT-PCR	RcS₁-RNase	F：GCGTTTAGTCTGCAATGACGCAGAAGCTC;R：ACTAGTATCAAACGAAATGATTGCTCGCG
	RcS₂-RNase	F：TGTGGTAACAGCTGCAAAGC;R：TCAACCACGTTTTTGCCATA
	Actin	F：GGCTGTTCTCTCTCTGTATGC;R：TTCTGGGCACCTGAATCTC

授粉 Pollination	材料 Material	授粉花朵数 Number of pollinated flowers	收获果实数 Number of fruits	坐果率/% Fruit-set ration	种子数 Number of seeds
自花授粉 Self-pollination	2015-58-20（P1）	20	0	0	0
自花授粉 Self-pollination	月月红（P2） Slater’s Crimson China	51	0	0	0
异花授粉 Cross-pollination	P1 × P2	235	96	40.86	362

授粉 Pollination	材料 Material	授粉花朵数 Number of pollinated flowers	收获果实数 Number of fruits	坐果率/% Fruit-set ration	种子数 Number of seeds
自花授粉 Self-pollination	2015-58-20（P1）	20	0	0	0
自花授粉 Self-pollination	月月红（P2） Slater’s Crimson China	51	0	0	0
异花授粉 Cross-pollination	P1 × P2	235	96	40.86	362

蛋白 Protein	长度/aa Length	分子量/kD MW	等电点 pI	天冬氨酸 + 谷氨酸 Asp（-）+ Glu（-）	精氨酸 + 赖氨酸 Arg（+）+ Lys（+）	不稳定性系数 Instablility index	脂肪族氨基酸系数 Aliphatic aa index	亲疏水性 Hydropathicity
RcS₁-RNase	206	24.04	8.74	20	25	37.78	67.23	-0.66
RcS₂-RNase	219	24.84	7.57	19	20	35.73	71.28	-0.30

Identification and Analysis of Self Incompatibility S-RNase in Rose

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