Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (1): 157-165.doi: 10.16420/j.issn.0513-353x.2020-0758

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Identification and Analysis of Self Incompatibility S-RNase in Rose

LI Maofu1,2,3, YANG Yuan1,2,4, WANG Hua1,2,3, FAN Youwei1,2,3, SUN Pei1,2,3, JIN Wanmei1,2,3,*()   

  1. 1Institute of Forestry and Pomology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100093,China
    2Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Ministry of Agriculture,Beijing 100093,China
    3Beijing Engineering Research Center of Functional Floriculture,Beijing 100093,China
    4Beijing Engineering Research Center for Deciduous Fruit Trees,Beijing 100093,China
  • Received:2021-03-30 Revised:2021-09-07 Online:2022-01-25 Published:2022-01-24
  • Contact: JIN Wanmei


In Rosa chinensis‘Slater’s Crimson China’and Rosa sp.‘2015-58-20’,the fluorescence microscope observation of pollen tubes in both self-pollinated and cross-pollinated styles indicated that rose is a typical gametophyte self-incompatible plant. The full-length sequence of RcS1-RNase and RcS2-RNase cDNA and DNA have been identified in the‘Slater’s Crimson China’. Sequence analysis showed that RcS1-RNase and RcS2-RNase have five conserved domains(C1,C2,C3,RC4 and C5)and a hypervariable region. Phylogenetic analysis found that RcS-RNase has high homology with S-RNase proteins in other Rosaceae plants. Tissue specific expression analysis indicated that RcS1-RNase and RcS2-RNase genes are solely expressed in styles.

Key words: Rosa, self incompatibility, S-RNase, gene cloning, gene expression

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