Fig. 1 Dynamic development of three sexual organs of grape S1，S2，and S3 represent early bud stage，large bud stage and blooming stage，respectively. The same below.

Fig. 2 Observation of different sex grapes pollen morphology by electron microscopy

Fig. 3 Endogenous hormone content in three sexual organs of grape The different letters are significantly different between samples（LSD，P ≤ 0.05）.

Fig. 4 Statistics of differentially expressed gene numbers among different stages in three sexual organs of grape

Fig. 5 Heatmap of MADS-box gene expression patterns in three sexual organs of grape

Fig. 6 Statistics of differentially expressed gene numbers among the three types of floral organ at the same stage F，H，and M represent female，hermaphrodite and male.

Fig. 7 GO（biological process）enrichment of differentially expressed genes involved in three types of grape flower development at the same stage（top 10） The numbers on the right indicate the significantly enriched genes.

Fig. 8 Hierarchical clustering tree of differentially expressed genes involved in floral organ forming at three development stages in female（F），hermaphrodite（H）and male（M） Genes presented are involved in the formation of sepals，petal，stamen，gynoecium and carpel.

Fig. 9 Hierarchical clustering tree of differentially expressed genes involved in the flowering pathways of grape flower with three sexes in early bud stage（S1），large bud stage（S2）and blooming stage（S3） Genes shown are involved in photoperiod pathway，vernalization pathway，autonomuspathway，and plant hormone signal transduction pathways.

Fig. 10 Venn dendragrams of differentially expressed genes between male and female flowers

Fig. 11 qPCR validation of genes involved in grape flower development and sex specification