Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (12): 2601-2618.doi: 10.16420/j.issn.0513-353x.2022-0769
• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles
WANG Xuanzhen, CHEN Mindong, LIU Jianting, ZENG Meijuan, YE Xinru, LI Yongping, WEN Qingfang, ZHU Haisheng*()
Received:
2023-10-18
Revised:
2023-12-10
Online:
2023-12-25
Published:
2023-12-29
Contact:
ZHU Haisheng
WANG Xuanzhen, CHEN Mindong, LIU Jianting, ZENG Meijuan, YE Xinru, LI Yongping, WEN Qingfang, ZHU Haisheng. Identification of Luffa Under Low Temperature and Weak Light Stress Gene Co-expression Modules by WGCNA[J]. Acta Horticulturae Sinica, 2023, 50(12): 2601-2618.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2022-0769
Fig. 1 Power value curve diagram The red line indicates a correlation coefficient of 0.9 and the blue line indicates a correlation coefficient of 0.8.
基因ID Gene | 正向引物(5′-3′) Forward sequence | 反向引物(5′-3′) Reverse sequence | 参考文献 Reference |
---|---|---|---|
18S | GTGTTCTTCGGAATGACTGG | ATCGTTTACGGCATGGACTA | 朱海生 等, |
Maker00022921 | CCTTCGGCTGTGGGTTAT | GTCAAATCGTCCGCAGGT | |
Maker00001558 | GGCGTTTCAATCCCAGAG | AAGCGACTTCAGTTCACC | |
Maker00035320 | TTTGGGTATGGCTTGTGATG | AAACAGTCGTGGAAATGGAG |
Table 1 Primer information
基因ID Gene | 正向引物(5′-3′) Forward sequence | 反向引物(5′-3′) Reverse sequence | 参考文献 Reference |
---|---|---|---|
18S | GTGTTCTTCGGAATGACTGG | ATCGTTTACGGCATGGACTA | 朱海生 等, |
Maker00022921 | CCTTCGGCTGTGGGTTAT | GTCAAATCGTCCGCAGGT | |
Maker00001558 | GGCGTTTCAATCCCAGAG | AAGCGACTTCAGTTCACC | |
Maker00035320 | TTTGGGTATGGCTTGTGATG | AAACAGTCGTGGAAATGGAG |
Fig. 4 The Correlation Map of specificity of low temperature and weak light The graph is made by Pearson Correlation Coefficient. The deeper the color,the stronger the correlation. The lower the number in the brackets,the more significant P value. The lower the value,the stronger the significance.
Fig. 7 The interaction network of three hub genes A:The coexpression network of ATPβ transcription factor(ID: Maker00022921)was used as the core gene;B:Coexpression network of PER5(ID:Maker00035320);C:Coexpression network of G6PDH(ID:Maker00001558). The graph size represents gene connectivity.
基因名称 Gene ID | 鉴定基因名称 Gene | 功能注释 Description | 相关性E-value | 相似度/% Perident | NCBI登记号 Accession | 物种 Species |
---|---|---|---|---|---|---|
Maker00001558 | G6PDH | 葡萄糖-6-磷酸脱氢酶 Glucose-6-phosphatedehy- drogenas | 0 | 95.74 | XP_008442193.1 | 甜瓜 Cucumismelo L. |
0 | 94.77 | XP_023543556.1 | 西葫芦 Cucurbita pepo L. | |||
0 | 94.77 | XP_022928729.1 | 中国南瓜 Cucurbita moschata | |||
Maker00022921 | ATPβ | ATP合成酶β亚基 ATP Synthase β subunit | 0 | 100.00 | QIT04057.1 | 埃及丝瓜 Luffa aegyptiaca |
0 | 96.11 | TKS05977.1 | 银白杨 Populus alba | |||
0 | 95.91 | RWR97779.1 | 沉水樟Cinnamomum micranthum(Hay.)Hay | |||
Maker00035320 | PER5 | 木质素阴离子过氧化物酶 Lignin peroxidase | 0 | 91.48 | XP_022931633.1 | 中国南瓜 Cucurbita moschata |
0 | 91.08 | XP_022158970.1 | 苦瓜 Momordica charantia L. | |||
0 | 87.97 | XP_038876931.1 | 冬瓜 Benincasa hispida (Thunb.)Cogn |
Table 2 Results of protein homology analysis of three hub genes
基因名称 Gene ID | 鉴定基因名称 Gene | 功能注释 Description | 相关性E-value | 相似度/% Perident | NCBI登记号 Accession | 物种 Species |
---|---|---|---|---|---|---|
Maker00001558 | G6PDH | 葡萄糖-6-磷酸脱氢酶 Glucose-6-phosphatedehy- drogenas | 0 | 95.74 | XP_008442193.1 | 甜瓜 Cucumismelo L. |
0 | 94.77 | XP_023543556.1 | 西葫芦 Cucurbita pepo L. | |||
0 | 94.77 | XP_022928729.1 | 中国南瓜 Cucurbita moschata | |||
Maker00022921 | ATPβ | ATP合成酶β亚基 ATP Synthase β subunit | 0 | 100.00 | QIT04057.1 | 埃及丝瓜 Luffa aegyptiaca |
0 | 96.11 | TKS05977.1 | 银白杨 Populus alba | |||
0 | 95.91 | RWR97779.1 | 沉水樟Cinnamomum micranthum(Hay.)Hay | |||
Maker00035320 | PER5 | 木质素阴离子过氧化物酶 Lignin peroxidase | 0 | 91.48 | XP_022931633.1 | 中国南瓜 Cucurbita moschata |
0 | 91.08 | XP_022158970.1 | 苦瓜 Momordica charantia L. | |||
0 | 87.97 | XP_038876931.1 | 冬瓜 Benincasa hispida (Thunb.)Cogn |
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