Cloning，Expression and Promoter Activity Analysis of VlRRA1 Gene in Grape

doi:10.16420/j.issn.0513-353x.2022-0516

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (8): 1609-1621.doi: 10.16420/j.issn.0513-353x.2022-0516

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Next Articles

Cloning，Expression and Promoter Activity Analysis of VlRRA1 Gene in Grape

LI Songqi, LI Xufei, LI Min, LIU Hainan, PEI Maosong, WEI Tonglu, GUO Dalong, YU Yihe^*()

Henan Engineering Technology Research Center of Quality Regulation and Controlling of Horticultural Plants，College of Horticulture and Plant Protection，Henan University of Science and Technology，Luoyang，Henan 471023，China

Received:2023-03-24 Revised:2023-04-21 Online:2023-08-25 Published:2023-08-23
Contact: YU Yihe

Abstract

Abstract:

The cytokinin response regulator VlRRA1 and its promoter were cloned from‘Kyoho’grape. The expression characteristics of VlRRA1 and the activity of VlRRA1 promoter were analyzed. The results showed that the full-length cDNA of VlRRA1 was 666 bp and encoded 221 amino acids. Conservative domain analysis showed that the gene contained only one phosphoacceptor receiver domain（REC），which belongs to type-A RR genes and can only accept phosphate groups;phylogenetic relationships showed that VlRRA1 was closely related to type-A RR in other species. The yeast self-activation assay illustrates that VlRRA1 has no transcriptional activation activity in agreement with other A-type RR genes and does not have a function similar to that of the B-type RR transcription factor. The results of qRT-PCR showed that VlRRA1 had tissue-specific expression and was mainly expressed in stems，leaves and young fruit，slightly lower expression in inflorescence and ripe fruit，suggesting that VlRRA1 may play an important role in the process of fruit set;exogenous cytokinin forchlorfenuron（CPPU）can promote VlRRA1 expression，while the cytokinin biosynthesis inhibitor lovastatin（LOV）inhibits VlRRA1 transcription. Many elements related to abiotic stress and hormone response were found in VlRRA1 promoter. The results of GUS tissue staining showed that VlRRA1 promoter had promoter activity and could respond to a variety of hormone signals，including three plant hormones related to fruit setting，cytokinin，auxin and gibberellin. These results suggest that VlRRA1 plays an important role in grape fruit setting and young fruit development mediated by cytokinin.

Key words: grape, cytokinin, cytokinin response regulator, VlRRA1, promoter activity

LI Songqi, LI Xufei, LI Min, LIU Hainan, PEI Maosong, WEI Tonglu, GUO Dalong, YU Yihe. Cloning，Expression and Promoter Activity Analysis of VlRRA1 Gene in Grape[J]. Acta Horticulturae Sinica, 2023, 50(8): 1609-1621.

Figures/Tables 10

References 46

[1]	Ahmad B, Zhang S, Yao J, Chai S, Yadav V, Athar H, Rahman M U, Wang L, Wang X. 2022. Ectopic expression of VvFUS3,B3-domain transcription factor,in tomato influences seed development via affecting endoreduplication and hormones. Horticultural Plant Journal, 8 (3):351-360. doi: 10.1016/j.hpj.2020.12.009 URL
[2]	Argyros R D, Mathews D E, Chiang Y, Palmer C M, Thibault D M, Etheridge N, Argyros D A, Mason M G, Kieber J J, Schaller G E. 2008. Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development. The Plant Cell, 20 (8):2102-2116. doi: 10.1105/tpc.108.059584 URL
[3]	Bhargava A, Clabaugh I, To J P, Maxwell B B, Chiang Y, Schaller G E, Loraine A, Kieber J J. 2013. Identification of cytokinin-responsive genes using microarray meta-analysis and RNA-Seq in Arabidopsis. Plant Physiology, 162 (1):272-294. doi: 10.1104/pp.113.217026 pmid: 23524861
[4]	Bian L, Shi B X, Yu K K, Guo D L, Ji X R, Ni P Y, Yang Y J, Zhang G H, Yu Y H. 2022. Genome-wide characterization of cytokinin response regulator in grape and expression analyses during berry set process. Russian Journal of Plant Physiology, 69 (3):1-13. doi: 10.1134/S1021443722010058
[5]	Bian Lu,Guo Dalong,Yu Keke,Wei Tonglu,Pei Maosong,Liu Hainan,Yu Yihe. 2021. Cloning and expression analysis of the cytokinin response regulator VlRR5 in Kyoho grapevine. Acta Horticulturae Sinica, 48 (8):1437-1445. (in Chinese)
	边璐, 郭大龙, 于可可, 韦同路, 裴茂松, 刘海楠, 余义和. 2021. ‘巨峰’葡萄细胞分裂素响应调节因子VlRR5的克隆与表达分析. 园艺学报, 48 (8):1437-1445.
[6]	Buechel S, Leibfried A, To J P, Zhao Z, Andersen S U, Kieber J J, Lohmann J U. 2010. Role of A-type ARABIDOPSIS RESPONSE REGULATORS in meristem maintenance and regeneration. European Journal of Cell Biology, 89 (2-3):279-284. doi: 10.1016/j.ejcb.2009.11.016 pmid: 20018401
[7]	D’Agostino I B, Deruere J, Kieber J J. 2000. Characterization of the response of the Arabidopsis response regulator gene family to cytokinin. Plant Physiology, 124 (4):1706-1717. doi: 10.1104/pp.124.4.1706 URL
[8]	Hartig K, Beck E. 2005. Assessment of lovastatin application as tool in probing cytokinin‐mediated cell cycle regulation. Physiologia Plantarum, 125 (2):260-267. doi: 10.1111/ppl.2005.125.issue-2 URL
[9]	Hass C, Lohrmann J, Albrecht V, Sweere U, Hummel F, Yoo S D, Hwang I, Zhu T, Schäfer E, Kudla J. 2004. The response regulator 2 mediates ethylene signalling and hormone signal integration in Arabidopsis. The EMBO Journal, 23 (16):3290-3302. doi: 10.1038/sj.emboj.7600337 URL
[10]	He Minjie, Tan Yao, Xie Jiangnan, Liu Zhong, Zhou Yong. 2018. Effects of several plant growth regulators for promoting setting of‘Kyoho’ grapevine. Sino-Overseas Grapevine & Wine,(4):74-77. (in Chinese)
	何敏杰, 谭瑶, 谢江南, 刘忠, 周勇. 2018. 几种植物生长调节剂促进‘巨峰'葡萄坐果的研究. 中外葡萄与葡萄酒,(4):74-77.
[11]	Hirose N, Makita N, Kojima M, Kamada-Nobusada T, Sakakibara H. 2007. Overexpression of a type-A response regulator alters rice morphology and cytokinin metabolism. Plant and Cell Physiology, 48 (3):523-539. doi: 10.1093/pcp/pcm022 pmid: 17293362
[12]	Hwang I, Sheen J. 2001. Two-component circuitry in Arabidopsis cytokinin signal transduction. Nature, 413 (6854):383-389. doi: 10.1038/35096500
[13]	Hwang I, Sheen J, Müller B. 2012. Cytokinin signaling networks. Annual Review of Plant Biology, 63:353-380. doi: 10.1146/annurev-arplant-042811-105503 pmid: 22554243
[14]	Ishida K, Yamashino T, Mizuno T. 2008a. Expression of the cytokinin-induced type-A response regulator gene ARR9 is regulated by the circadian clock in Arabidopsis thaliana. Bioscience,Biotechnology,and Biochemistry, 72 (11):3025-3029. doi: 10.1271/bbb.80402 URL
[15]	Ishida K, Yamashino T, Yokoyama A, Mizuno T. 2008b. Three type-B response regulators,ARR1,ARR10 and ARR12,play essential but redundant roles in cytokinin signal transduction throughout the life cycle of Arabidopsis thaliana. Plant and Cell Physiology, 49 (1):47-57. doi: 10.1093/pcp/pcm165 URL
[16]	Jain M, Tyagi A K, Khurana J P. 2006. Molecular characterization and differential expression of cytokinin-responsive type-A response regulators in rice(Oryza sativa). BMC Plant Biology, 6 (1):1-11. doi: 10.1186/1471-2229-6-1
[17]	Jeon J, Kim N Y, Kim S, Kang N Y, Novák O, Ku S, Cho C, Lee D J, Lee E, Strnad M. 2010. A subset of cytokinin two-component signaling system plays a role in cold temperature stress response in Arabidopsis. Journal of Biological Chemistry, 285 (30):23371-23386. doi: 10.1074/jbc.M109.096644 pmid: 20463025
[18]	Jia Ruirui. 2018. Gene cloning and expression analysis of canker-related transcription factor CsBZIP40 in citrus[Ph. D. Dissertation]. Chongqing:Southwest University. (in Chinese)
	贾瑞瑞. 2018. 柑橘溃疡病相关转录因子CsBZIP40的功能研究[博士论文]. 重庆: 西南大学.
[19]	Kieber J J, Schaller G E. 2018. Cytokinin signaling in plant development. Development, 145 (4):1-7.
[20]	Kurepa J, Li Y, Smalle J A. 2014. Cytokinin signaling stabilizes the response activator ARR1. The Plant Journal, 78 (1):157-168. doi: 10.1111/tpj.12458 pmid: 24617630
[21]	Li Y, Zhang D, Zhang L, Zuo X, Fan S, Zhang X, Shalmani A, Han M. 2017. Identification and expression analysis of cytokinin response-regulator genes during floral induction in apple (Malus domestica Borkh). Plant Growth Regulation, 83 (3):455-464. doi: 10.1007/s10725-017-0311-2 URL
[22]	Liu Jie,Yang Lina,Tao Jianmin,Xu Kuanyong,Cheng Qunkang,Liu Yanhong. 2008. Effects of GA₃ and CPPU on seedless development of new Manicure Finger grape fruit. South China Fruits,(1):61-62. (in Chinese)
	刘捷, 杨丽娜, 陶建敏, 许宽勇, 程群康, 刘艳红. 2008. GA₃与CPPU对新美人指葡萄果实无核化发育的影响. 中国南方果树,(1):61-62.
[23]	Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method. Methods, 25 (4):402-408. doi: 10.1006/meth.2001.1262 pmid: 11846609
[24]	Lomin S N, Krivosheev D M, Steklov M Y, Osolodkin D I, Romanov G A. 2012. Receptor properties and features of cytokinin signaling. Acta Naturae, 3 (14):31-45.
[25]	Lu Jing, Zhao Huayan, He Yikun, Song Yanru. 2004. Research progress of higher plant promoter and its application. Progress in Natural Science, 14 (8):856-862. (in Chinese)
	路静, 赵华燕, 何奕昆, 宋艳茹. 2004. 高等植物启动子及其应用研究进展. 自然科学进展, 14 (8):856-862.
[26]	Mariotti L, Picciarelli P, Lombardi L, Ceccarelli N. 2011. Fruit-set and early fruit growth in tomato are associated with increases in indoleacetic acid,cytokinin,and bioactive gibberellin contents. Journal of Plant Growth Regulation, 30 (4):405-415. doi: 10.1007/s00344-011-9204-1 URL
[27]	Mason M G, Li J, Mathews D E, Kieber J J, Schaller G E. 2004. Type-B response regulators display overlapping expression patterns in Arabidopsis. Plant Physiology, 135 (2):927-937. doi: 10.1104/pp.103.038109 URL
[28]	Matsushika A, Makino S, Kojima M, Mizuno T. 2000. Circadian waves of expression of the APRR1/TOC 1 family of pseudo-response regulators in Arabidopsis thaliana:insight into the plant circadian clock. Plant and Cell Physiology, 41(9):1002-1012. doi: 10.1093/pcp/pcd043 pmid: 11100772
[29]	McAtee P, Karim S, Schaffer R J, David K. 2013. A dynamic interplay between phytohormones is required for fruit development,maturation,and ripening. Frontiers in Plant Science, 4:1-7.
[30]	OIV. 2021. State of the World Vitivinicultural sector in 2020. Paris:OIV.
[31]	Pan Y J, Wang D, Zhu L H, Fu B Y, Li Z K. 2009. Differential expressions of two-component element genes in rice under drought stress. Acta Agronomica Sinica, 35 (9):1628-1636.
[32]	Ren B, Liang Y, Deng Y, Chen Q, Zhang J, Yang X, Zuo J. 2009. Genome-wide comparative analysis of type-A Arabidopsis response regulator genes by overexpression studies reveals their diverse roles and regulatory mechanisms in cytokinin signaling. Cell Research, 19 (10):1178-1190. doi: 10.1038/cr.2009.88 pmid: 19621034
[33]	Sakai H, Aoyama T, Oka A. 2000. Arabidopsis ARR1 and ARR2 response regulators operate as transcriptional activators. The Plant Journal, 24 (6):703-711.
[34]	Salome P A, To J P, Kieber J J, McClung C R. 2006. Arabidopsis response regulators ARR3 and ARR4 play cytokinin-independent roles in the control of circadian period. The Plant Cell, 18 (1):55-69. doi: 10.1105/tpc.105.037994 URL
[35]	Shen Jiaqi. 2021. Studies on selection of pathenocarpic fruit inducers for‘Cuiguan’pear and molecular mechanism of gibberellin-induced parthenocarpy[M. D. Dissertation]. Hangzhou: Zhejiang University. (in Chinese)
	沈家琪. 2021. ‘翠冠’梨单性结实诱导剂筛选及赤霉素诱导单性结实坐果分子机理研究[硕士论文]. 杭州: 浙江大学.
[36]	To J P, Haberer G, Ferreira F J, Deruere J, Mason M G, Schaller G E, Alonso J M, Ecker J R, Kieber J J. 2004. Type-A Arabidopsis response regulators are partially redundant negative regulators of cytokinin signaling. The Plant Cell, 16 (3):658-671. doi: 10.1105/tpc.018978 URL
[37]	Xu Weirong. 2010. Cloning and functional analysis of stilbene synthase and its promoter from powdery mildewresistant Chinese Vitis pseudoreticulata [Ph. D. Dissertation]. Yangling:Northwest A & F University. (in Chinese)
	徐伟荣. 2010. 中国华东葡萄抗白粉病芪合成酶基因及启动子克隆与功能分析[博士论文]. 杨凌: 西北农林科技大学.
[38]	Yang Y, Jiang Y, Mi X, Gan L, Gu T, Ding J, Li Y. 2016. Identification and expression analysis of cytokinin response regulators in Fragaria vesca. Acta Physiologiae Plantarum, 38:1-13. doi: 10.1007/s11738-015-2023-4 URL
[39]	Yu Keke, Yang Yingjun, Guo Dalong, Liu Hainan, Pei Maosong, Wei Tonglu, Bian Lu, Yu Yihe. 2021. Cloning and plant growth regulator response analysis of VlCKX8 in grape. Plant Physiology Journal, 57 (1):85-93. (in Chinese)
	于可可, 杨英军, 郭大龙, 刘海楠, 裴茂松, 韦同路, 边璐, 余义和. 2021. 葡萄VlCKX8基因克隆与植物生长调节剂响应分析. 植物生理学报, 57 (1):85-93.
[40]	Yu Y, Bian L, Yu K, Yang S, Zhang G, Guo D. 2020. Grape (Vitis davidii) VdGATA2 functions as a transcription activator and enhances powdery mildew resistance via the active oxygen species pathway. Scientia Horticulturae, 267:1-8.
[41]	Yu Y, Li X, Yang S, Bian L, Yu K, Meng X, Liu H, Pei M, Wei T, Guo D. 2021. CPPU-induced changes in energy status and respiration metabolism of grape young berry development in relation to berry setting. Scientia Horticulturae, 283:1-7.
[42]	Zabadal T J, Bukovac M J. 2006. Effect of CPPU on fruit development of selected seedless and seeded grape cultivars. HortScience, 41 (1):154-157. doi: 10.21273/HORTSCI.41.1.154 URL
[43]	Zhang C, Fan X, Liu C, Fang J. 2021. Anatomical berry characteristics during the development of grape berries with different shapes. Horticultural Plant Journal, 7 (4):295-306. doi: 10.1016/j.hpj.2021.04.002 URL
[44]	Zhang Hongmei, Wang Junli, Liao Xiangru. 2003. Biosynthesis metabolism and receptor of cytokinins. Plant Physiology Journal, 39 (3):267-272. (in Chinese)
	张红梅, 王俊丽, 廖祥儒. 2003. 细胞分裂素的生物合成、代谢和受体. 植物生理学报, 39 (3):267-272.
[45]	Zhang Weiwei, Yang Yongzhen. 2006. Advances in research and application of forchlorfenuron. Pesticide Science and Administration, 27 (5):36-40. (in Chinese)
	张卫炜, 杨永珍. 2006. 氯吡脲的研究及应用进展. 农药科学与管理, 27 (5):36-40.
[46]	Zhang Wenchao, Hu Guang. 1994. Study on the cause of fructuary drop of Kyoho grape. Northern Horticulture,(5):35-36. (in Chinese)
	张文超, 胡光. 1994. 巨峰葡萄落花落果原因的研究. 北方园艺,(5):35-36.

用途 Use	引物名称 Name of primer	引物序列（5′-3′） Primer sequence
VlRRA1克隆	VlRRA1-F	atggccatggaggccgaattcATGGAGATAAGCGGTGTTCT
Cloning of VlRRA1 sequence	VlRRA1-R	atgcggccgctgcaggaattcCTAATCACATGTGGAGGTTT
实时荧光定量PCR	qPCR-VlRRA1-F	GACAGATGTTTGGAGGAAGGTG
qRT-PCR	qPCR-VlRRA1-R	TGAAGATATGGACGGTGGTGAT
VlRRA1启动子克隆	ProVlRRA1-F	tgggcccggcgcgccaagcttGGTCATTTCATTGCTTTCCTCTC
Cloning of VlRRA1 promoter sequence	ProVlRRA1-R	ggtggactcctcttagaattcACAGGGTTCCGTGATTCTGTG

用途 Use	引物名称 Name of primer	引物序列（5′-3′） Primer sequence
VlRRA1克隆	VlRRA1-F	atggccatggaggccgaattcATGGAGATAAGCGGTGTTCT
Cloning of VlRRA1 sequence	VlRRA1-R	atgcggccgctgcaggaattcCTAATCACATGTGGAGGTTT
实时荧光定量PCR	qPCR-VlRRA1-F	GACAGATGTTTGGAGGAAGGTG
qRT-PCR	qPCR-VlRRA1-R	TGAAGATATGGACGGTGGTGAT
VlRRA1启动子克隆	ProVlRRA1-F	tgggcccggcgcgccaagcttGGTCATTTCATTGCTTTCCTCTC
Cloning of VlRRA1 promoter sequence	ProVlRRA1-R	ggtggactcctcttagaattcACAGGGTTCCGTGATTCTGTG

顺式作用元件	序列	功能	数量
cis-acting element	Sequence	Function	Number
CAAT-box	CCAAT	启动子和增强子区域常见的顺式作用元件	14
		Common cis-acting element in promoter and enhancer regions
TATA-box	TATAAAT/TATA	转录起始位点周围 - 30左右的核心启动子元件	28
		Core promoter element around -30 of transcription start
TCA-element	CCATCTTTTT	参与水杨酸响应的顺式作用元件	1
		cis-Acting element involved in salicylic acid responsiveness
TGACG/CGTCA-motif	TGACG/CGTCA	参与茉莉酸甲酯响应的顺式作用调节元件	4
		cis-Acting regulatory element involved in the MeJA-responsiveness
ARE	AAACCA	厌氧诱导的关键顺式作用调节元件	2
		cis-Acting regulatory element essential for the anaerobic induction
LTR	CCGAAA	参与低温响应的顺式作用元件	1
		cis-Acting element involved in low-temperature responsiveness
MBS	CAACTG	参与干旱诱导的MYB结合位点	2
		MYB binding site involved in drought-inducibility
circadian	CAAAGATATC	参与昼夜节律控制的顺式调控元件	1
		cis-Acting regulatory element involved in circadian control
Box 4	ATTAAT	光响应相关的保守DNA模块的一部分	1
		Part of the conserved DNA module involved in light responsiveness
G-box	CACGAC	参与光响应的顺式作用调节元件	1
		cis-Acting regulatory element involved in light responsiveness
3-AF1 binding site	TAAGAGAGGAA	光响应元件 Light responsive element	1
GT1-motif	GGTTAA	光响应元件 Light responsive element	2
Sp1	GGGCGG	光响应元件 Light responsive element	1
GA-motif	ATAGATAA	光响应元件的一部分 Part of the light responsive element	1
GTGGC-motif	GATTCTGTGGC	光响应元件的一部分 Part of the light responsive element	1

顺式作用元件	序列	功能	数量
cis-acting element	Sequence	Function	Number
CAAT-box	CCAAT	启动子和增强子区域常见的顺式作用元件	14
		Common cis-acting element in promoter and enhancer regions
TATA-box	TATAAAT/TATA	转录起始位点周围 - 30左右的核心启动子元件	28
		Core promoter element around -30 of transcription start
TCA-element	CCATCTTTTT	参与水杨酸响应的顺式作用元件	1
		cis-Acting element involved in salicylic acid responsiveness
TGACG/CGTCA-motif	TGACG/CGTCA	参与茉莉酸甲酯响应的顺式作用调节元件	4
		cis-Acting regulatory element involved in the MeJA-responsiveness
ARE	AAACCA	厌氧诱导的关键顺式作用调节元件	2
		cis-Acting regulatory element essential for the anaerobic induction
LTR	CCGAAA	参与低温响应的顺式作用元件	1
		cis-Acting element involved in low-temperature responsiveness
MBS	CAACTG	参与干旱诱导的MYB结合位点	2
		MYB binding site involved in drought-inducibility
circadian	CAAAGATATC	参与昼夜节律控制的顺式调控元件	1
		cis-Acting regulatory element involved in circadian control
Box 4	ATTAAT	光响应相关的保守DNA模块的一部分	1
		Part of the conserved DNA module involved in light responsiveness
G-box	CACGAC	参与光响应的顺式作用调节元件	1
		cis-Acting regulatory element involved in light responsiveness
3-AF1 binding site	TAAGAGAGGAA	光响应元件 Light responsive element	1
GT1-motif	GGTTAA	光响应元件 Light responsive element	2
Sp1	GGGCGG	光响应元件 Light responsive element	1
GA-motif	ATAGATAA	光响应元件的一部分 Part of the light responsive element	1
GTGGC-motif	GATTCTGTGGC	光响应元件的一部分 Part of the light responsive element	1

Cloning，Expression and Promoter Activity Analysis of VlRRA1 Gene in Grape

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[14]	WANG Baoliang, LIU Fengzhi, JI Xiaohao, WANG Xiaodi, SHI Xiangbin, ZHANG Yican, LI Peng, and WANG Haibo. A New Early Ripening Grape Cultivar‘Huapu Zaoyu’for Table [J]. Acta Horticulturae Sinica, 2022, 49(S2): 33-34.
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