Investigation of the Effect of Rain-shelter Cultivation on the Microbial Diversity of Berry Surface for‘Cabernet Sauvignon’Grapes

doi:10.16420/j.issn.0513-353x.2021-1190

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (3): 635-646.doi: 10.16420/j.issn.0513-353x.2021-1190

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Investigation of the Effect of Rain-shelter Cultivation on the Microbial Diversity of Berry Surface for‘Cabernet Sauvignon’Grapes

HUANG Rong¹, DONG Chao¹, JIANG Jiao¹, QIN Yi¹^,², LIU Yanlin¹^,², SONG Yuyang¹^,²^,^*()

¹College of Enology，Northwest A & F University，Yangling，Shaanxi 712100，China
²Ningxia Helan Mountain’s East Foothill Wine Experiment and Demonstration Station，Northwest A & F University，Yongning，Ningxia 750104，China

Received:2022-11-26 Revised:2023-01-04 Online:2023-03-25 Published:2023-04-03
Contact: *（E-mail：yuyangsong@nwsuaf.edu.cn）

Abstract

Abstract:

This study aimed to investigate the effect of rain-shelter cultivation on the microbial diversity of Cabernet Sauvignon grapes at veraison and ripening stages via high-throughput sequencing. Ascomycota（percentage > 90%） and Proteobacteria were the dominant fungal and bacterial phylum in both rain-shelter and open-field cultivation patterns. Compared with grapes being cultivated in the open-field，rain-shelter reduced the richness of Colletotrichum，therefore decreasing the risk of developing anthracnose on grape berries. In this scenario，a decreased abundance of Alternaria，which was associated with berry rot and secretion of killer toxins，was also seen. Additionally，rain-shelter conditions were more suitable for the development of Pseudomonas and Pantoea，both bacteria are capable of nitrogen-fixing. The abundance of Aspergillus and Trichothecium were commonly higher in the rain-shelter treatment than in the control. NMDS analysis with Welch’s t test suggested that rain-shelter resulted in significant differences in fungal and bacterial community structure at the veraison stage（stress < 0.1；P < 0.05）. In summary，rain-shelter enhanced the colonization of microbes that are beneficial to vine growth and grape quality whilst also reducing the richness of microorganisms that could potentially cause diseases affecting grape health.

Key words: wine grape, rain-shelter cultivation, microbial community, diversity analysis, high-throughput sequencing analysis

CLC Number:

S663.1

HUANG Rong, DONG Chao, JIANG Jiao, QIN Yi, LIU Yanlin, SONG Yuyang. Investigation of the Effect of Rain-shelter Cultivation on the Microbial Diversity of Berry Surface for‘Cabernet Sauvignon’Grapes[J]. Acta Horticulturae Sinica, 2023, 50(3): 635-646.

Figures/Tables 7

Fig. 1 Composition of fungal（a，b）and bacterial（c，d）communities on the surface of‘Cabernet Sauvignon’grapes cultivated in rain-shelter and open -field modes a and c：at phylum level；b and d：at genus level. S：Rain-shelter mode；N：Open-field mode（control）. V：Veraison stage；R：Ripening stage. The same below.

Fig. 2 Fungal（a）and bacterial（b）OTUs shared or specific on the surface of‘Cabernet Sauvignon’grapes cultivated in rain-shelter and open-fields

Table 1 α diversity of bacteria and fungi on the surface of‘Cabernet Sauvignon’grapes cultivated in rain-shelter and open-fields

处理 Treatment	采收时期 Sampling stage	真菌 Fungi
处理 Treatment	采收时期 Sampling stage	Sobs	Chao1	Simpson	覆盖率 Good’s coverage
避雨棚	转色期 Veraison stage	574.33	691.88	0.78	1.00
Rain-shelter	成熟期 Ripening stage	302.00	432.71	0.79	1.00
露天（对照）	转色期 Veraison stage	455.00	572.37	0.81	1.00
Open-field（Control）	成熟期 Ripening stage	301.00	423.71	0.82	1.00
处理 Treatment	采收时期 Sampling stage	细菌 Bacteria
处理 Treatment	采收时期 Sampling stage	Sobs	Chao1	Simpson	覆盖率 Good’s coverage
避雨棚	转色期 Veraison stage	1 274.33	2 034.17	0.88	1.00
Rain-shelter	成熟期 Ripening stage	414.50	624.63	0.67	1.00
露天（对照）	转色期 Veraison stage	808.17	1 194.15	0.96	1.00
Open-field（Control）	成熟期 Ripening stage	480.33	774.99	0.63	1.00

Fig. 3 PcoA analysis of fungal（a）and bacterial（b）community structure on the surface of‘Cabernet Sauvignon’grapes cultivated in rain-sheltered and open-field modes

Fig. 4 NMDS analysis of fungal（a）and bacterial（b）community structure on the surface of‘Cabernet Sauvignon’grapes cultivated in rain-sheltered and open-field modes

Fig. 5 Welch’s t test for differences in fungal（a）and bacterial（b）species on the surface of‘Cabernet Sauvignon’grapes in rain-shelter and open-field modes during veraison stage（P < 0.05）

Fig. 6 Detection of fungal（a）and bacterial（b）species differences on the surface of‘Cabernet Sauvignon’grapes in rain-shelter and open-field modes during ripening a：LEFse analysis of fungal flora（LDA > 3）；b：Welch’s t test of significant differences in bacterial communities（P < 0.05）。

References 37

[1]	Ali A, Ghani M, Li Y, Ding H, Meng H, Cheng Z. 2019. Hiseq base molecular characterization of soil microbial community,diversity structure,and predictive functional profiling in continuous cucumber planted soil affected by diverse cropping systems in an intensive greenhouse region of northern China. International Journal of Molecular Sciences, 20 (11):2619. doi: 10.3390/ijms20112619 URL
[2]	Aragon I, Perez-Mendoza D, Gallegos M, Ramos C. 2015. The c-di-GMP phosphodiesterase BifA is involved in the virulence of bacteria from the Pseudomonas syringae complex. Molecular Plant Pathology, 16 (6):604-615. doi: 10.1111/mpp.2015.16.issue-6 URL
[3]	Barata A, Malfeito-Ferreira M, Loureiro V. 2012. The microbial ecology of wine grape berries. International Journal of Food Microbiology, 153 (3):243-259. doi: 10.1016/j.ijfoodmicro.2011.11.025 pmid: 22189021
[4]	Bedimo J, Cilas C, Nottéghem J, Bieysse D. 2012. Effect of temperatures and rainfall variations on the development of coffee berry disease caused by Colletotrichum kahawae. Crop Protection, 31 (1):125-131. doi: 10.1016/j.cropro.2011.09.010 URL
[5]	Blättel V, Wirth K, Claus H, Schlott B, Pfeiffer P, König H. 2009. A lytic enzyme cocktail from Streptomyce ssp. B 578 for the control of lactic and acetic acid bacteria in wine. Applied Microbiology & Biotechnology, 83 (5):839-848.
[6]	Bokulich N, Thorngate J, Richardson P, Mills D. 2014. Microbial biogeography of wine grapes is conditioned by cultivar,vintage,and climate. Proceedings of the National Academy of Sciences of the United States of America, 111 (1):E139-E148.
[7]	Cabral L, Rodríguez A, Delgado J, Patriarca A. 2019. Understanding the effect of postharvest tomato temperatures on two toxigenic Alternaria spp. strains:growth,mycotoxins and cell-wall integrity-related gene expression. Journal of the Science of Food and Agriculture, 99 (15):6689-6695. doi: 10.1002/jsfa.9950 pmid: 31350766
[8]	Caporaso J, Kuczynski J, Stombaugh J, Bittinger K, Bushman F, Costello E, Fierer N, Pena A, Goodrich J, Gordon J. 2010. QIIME allows analysis of high-throughput community sequencing data. Nature Methods, 7 (5):335-336. doi: 10.1038/nmeth.f.303 pmid: 20383131
[9]	Chen X, Zhang Q, Zeng S, Chen Y, Huang X. 2020. Rain-shelter cultivation affects fruit quality of pear,and the chemical properties and microbial diversity of rhizosphere soil. Canadian Journal of Plant Science, 100 (6):683-691. doi: 10.1139/cjps-2018-0249 URL
[10]	Ciani M, Comitini F, Ilaria M, Paola D. 2010. Controlled mixed culture fermentation:a new perspective on the use of non-Saccharomyces yeasts in winemaking. Fems Yeast Research,(2):123-133.
[11]	Cruz-Lagunas B, Ortega-Acosta S, Reyes-García G, Toribio-Jiménez J, Palemón-Alberto F. 2020. Colletotrichum gloeosporioides causes anthracnose on grapefruit(Citrus paradisi)in Mexico. Australasian Plant Disease Notes, 15 (1):31. doi: 10.1007/s13314-020-00401-z
[12]	Gao F, Chen J, Xiao J, Cheng W, Zheng X, Wang B, Shi X. 2019. Microbial community composition on grape surface controlled by geographical factors of different wine regions in Xinjiang,China. Food Research International, 122:348-360. doi: 10.1016/j.foodres.2019.04.029 URL
[13]	Grangeteau C, Roullier-Gall C, Rousseaux S, Gougeon R, Schmitt-Kopplin P, Alexandre H, Guilloux-Benatier M. 2017. Wine microbiology is driven by vineyard and winery anthropogenic factors. Microbial Biotechnology, 10:354-370. doi: 10.1111/1751-7915.12428 pmid: 27778455
[14]	Guetsky R, Kobiler I, Wang X, Perlman N, Prusky D. 2005. Metabolism of the flavonoid epicatechin by laccase of Colletotrichum gloeosporioides and its effect on pathogenicity on avocado fruits. Phytopathology, 95 (11):1341-1348. doi: 10.1094/PHYTO-95-1341 pmid: 18943366
[15]	Heredia-Ponce Z, de Vicente A, Cazorla F, Gutiérrez-Barranquero J. 2021. Beyond the wall:exopolysaccharides in the biofilm lifestyle of pathogenic and beneficial plant-associated Pseudomonas. Microorganisms, 9 (2):445. doi: 10.3390/microorganisms9020445 URL
[16]	Hong Y, Park H. 2013. Role of non-Saccharomyces yeasts in Korean wines produced from campbell early grapes:potential use of Hanseniaspora uvarum as a starter culture. Food Microbiology, 34 (1):207-214. doi: 10.1016/j.fm.2012.12.011 URL
[17]	Huang Z, Qiu J, Li J, Xu D, Liu Q. 2021. Exploration of microbial diversity based on 16S rRNA gene sequence analysis. Acta Microbiologica Sinica, 61 (5):1044-1063.
[18]	Jeromel A, Korenika A, Ivana T. 2019. An influence of different yeast species on wine aroma composition. The Science of Beverages, 5:171-285.
[19]	Kalua C, Boss P. 2009. Evolution of volatile compounds during the development of Cabernet Sauvignon grapes(Vitis vinifera L.). Journal of Agricultural and Food Chemistry, 57 (9):3818-3830. doi: 10.1021/jf803471n URL
[20]	King E, Bassi A, Ross D, Druebbisch B. 2011. An industry perspective on the use of“atoxigenic”strains of Aspergillus flavus as biological control agents and the significance of cyclopiazonic acid. Toxin Reviews, 30 (2-3):33-41. doi: 10.3109/15569543.2011.588818 URL
[21]	Liu D, Howell K. 2020. Community succession of the grapevine fungal microbiome in the annual growth cycle. Environmental Microbiology, 23 (4):1842-1857. doi: 10.1111/emi.v23.4 URL
[22]	Mezzasalma V, Sandionigi A, Bruni I, Bruno A, Lovicu G, Casiraghi M. 2017. Grape microbiome as a reliable and persistent signature of field origin and environmental conditions in Cannonau wine production. PLoS ONE, 12 (9):e0184615. doi: 10.1371/journal.pone.0184615 URL
[23]	Moreira N, Mendes F, de Pinho R, Hogg T, Vasconcelos I. 2008. Heavy sulphur compounds,higher alcohols and esters production profile of Hanseniaspora uvarum and Hanseniaspora guilliermondii grown as pure and mixed cultures in grape must. International Journal of Food Microbiology, 124 (3):231-238. doi: 10.1016/j.ijfoodmicro.2008.03.025 pmid: 18457893
[24]	Morrison-Whittle P, Goddard M. 2018. From vineyard to winery:a source map of microbial diversity driving wine fermentation. Environmental Microbiology, 20:75-84. doi: 10.1111/1462-2920.13960 pmid: 29052965
[25]	Nilgün N, Eniz K. 2019. Antibacterial effect of verjuice against food-borne pathogens. British Food Journal, 121 (10):2265-2276. doi: 10.1108/BFJ-11-2018-0746
[26]	Nisiotou A, Rantsiou K, Iliopoulos V, Cocolin L, Nychas G. 2011. Bacterial species associated with sound and Botrytis-infected grapes from a greek vineyard. International Journal of Food Microbiology, 145 (2-3):432-436. doi: 10.1016/j.ijfoodmicro.2011.01.017 pmid: 21315469
[27]	Pauline R, Amélie R, Laurence G. 2019. From flavanols biosynthesis to wine tannins:what place for grape seeds? Journal of Agricultural & Food Chemistry, 67 (5):1325-1343.
[28]	Portillo M, Mas A. 2016. Analysis of microbial diversity and dynamics during wine fermentation of Grenache grape variety by high-throughput barcoding sequencing. LWT-Food Science and Technology, 72:317-321. doi: 10.1016/j.lwt.2016.05.009 URL
[29]	Prieto C, Jara C, Mas A, Romero J. 2007. Application of molecular methods for analysing the distribution and diversity of acetic acid bacteria in Chilean vineyards. International Journal of Food Microbiology, 115 (3):348-355. pmid: 17289199
[30]	Schilder A, Miles T, Gillett J, Jarosz A. 2013. The effect of environmental factors on infection of blueberry fruit by Colletotrichum acutatum. Plant Pathology, 62 (6):1238-1247. doi: 10.1111/ppa.2013.62.issue-6 URL
[31]	Serra R, Braga A, Venancio A. 2005. Mycotoxin-producing and other fungi isolated from grapes for wine production,with particular emphasis on ochratoxin A. Research in Microbiology, 156 (4):515-521. pmid: 15862450
[32]	Sumby K, Bartle L, Grbin P, Jiranek V. 2019. Measures to improve wine malolactic fermentation. Applied Microbiology & Biotechnology, 103:2033-2051.
[33]	Wang F, Saito S, Michailides T, Xiao C. 2021. Postharvest use of natamycin to control Alternaria rot on blueberry fruit caused by Alternaria alternata and A. arborescens. Postharvest Biology and Technology, 172. https://doi.org/10.1016/j.postharvbio.2020.111383.
[34]	Yu S, Liu C, Liang C, Zang C, Liu L, Wang H, Guan T. 2017. Effects of rain-shelter cultivation on the temporal dynamics of grape downy mildew epidemics. Journal of Phytopathology, 165 (5):331-341. doi: 10.1111/jph.2017.165.issue-5 URL
[35]	Zhang S, Chen X, Zhong Q, Huang Z, Bai Z. 2017. Relations among epiphytic microbial communities from soil,leaves and grapes of the grapevine. Frontiers in Life Science, 10 (1):73-83. doi: 10.1080/21553769.2017.1365776 URL
[36]	Zhang S W, Chen X, Zhong Q, Zhuang X, Bai Z. 2019. Microbial community analyses associated with nine varieties of wine grape carposphere based on high-throughput sequencing. Microorganisms, 7 (12):E668.
[37]	Zhang P, Fuentes S, Siebert T, Krstic M, Herderich M, Barlow E, Howell K. 2016. Terpene evolution during the development of Vitis vinifera L. cv. Shiraz grapes. Food Chemistry, 204:463-474. doi: 10.1016/j.foodchem.2016.02.125 URL

Investigation of the Effect of Rain-shelter Cultivation on the Microbial Diversity of Berry Surface for‘Cabernet Sauvignon’Grapes

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