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Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (7): 1389-1401.doi: 10.16420/j.issn.0513-353x.2022-0478

• Genetic & Breeding·Germplasm Resources·Molecular Biology •     Next Articles

Cloning and Functional Analysis of the CDS and Promoter of Synthase Gene STS19 in Chinese Wild Grapevine

LIU Hui, YIN Xiangjing, FANG Jinghao, GAO Min, LI Zhi, WANG Xiping*()   

  1. State Key Laboratory of Crop Stress Biology in Arid Areas,State Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China,Ministry of Agriculture and Rural Affairs,College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China
  • Received:2023-01-17 Revised:2023-04-07 Online:2023-07-25 Published:2023-07-26
  • Contact: WANG Xiping

Abstract:

VaSTS19 and VdSTS19 were cloned from Vitis amurensis Rupr.‘Tonghua 3’and V. davidii Foex.‘Tangwei’. Sequence analysis showed that the open reading frame of VaSTS19 and VdSTS19 were 1 179 bp,encoding 392 amino acid,and they were located on chromosome 16. The identity between VaSTS19 and VdSTS19 nucleotide and amino acid was 98.81% and 98.21%,respectively. Both VaSTS19 and VdSTS19 are located on the cytomembrane,cytoplasm and nucleus. The expression of STS19 gene and the synthesis and accumulation of resveratrol were significantly promoted when VaSTS19 and VdSTS19 were transferred into tomato by Agrobacterium-mediated method. The promoters of VaSTS19 and VdSTS19 were cloned from‘Tonghua 3’and‘Tangwei’,respectively. cis-Acting elements analysis showed that both promoters contained stress-related elements,hormone-related elements and light-related elements. A sequence alignment showed that the VaSTS19 promoter was demonstrated 60% homology with the VST2 promoter cloned from V. vinifera. The promoters of VaSTS19 and VdSTS19 and their fragments were significantly responsive to SA and MeJA induction in transgenic tobacco leaves. The results indicated that the expression pattern of STS19 and the production of resveratrol may be related to the regulation of the upstream region.

Key words: grape, resveratrol, STS19, functional analysis, promoter