Studies on Subcellular Localization and Expression in E.coli of MdWRKY from‘Qinguan’Apple

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (7): 1341-1348.

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Studies on Subcellular Localization and Expression in E.coli of MdWRKY from‘Qinguan’Apple

GAO Hua1,2，FAN Hong-ke1，DANG Zhi-guo1，WANG Fei1，WANG Lei-cun1,2，LIU Zhen-zhong1,2，and ZHAO Zheng-yang1,2,*

（1College of Horticulture，Northwest A & F University，Yangling，Shaanxi 712100，China；2Apple E & T Research Centre of Shaanxi Province，Yangling，Shaanxi 712100，China）

Received:2011-04-07 Revised:2011-06-08 Online:2011-07-25 Published:2011-07-25
Contact: ZHAO Zheng-yang1,2,*

Abstract

Abstract: A WRKY gene，designated as MdWRKY，was isolated from‘Qinguan’apple leaves using reverse transcription PCR approaches. MdWRKY was 1 224 bp and encoded a 331 amino-acid protein. Analysis of subcellular localization indicated that MdWRKY protein was targeted to the nucleus. Quantitative RT-PCR analysis showed that MdWRKY transcription was induced by Alternaria alternata f sp. mali indicating that MdWRKY gene may be involved in the interaction between plant and pathogen. Then expression in E. coli was conducted，SDS/PAGE result demonstrated that expression proteins consistent with the size of expected protein，which provided a foundation for further purifying and function study of MdWRKY. 　

Key words: apple, transcription factors, clone, subcellular localization, expression

CLC Number:

S 661.1

GAO Hua;FAN Hong-ke;DANG Zhi-guo;WANG Fei;WANG Lei-cun;LIU Zhen-zhong;and ZHAO Zheng-yang;. Studies on Subcellular Localization and Expression in E.coli of MdWRKY from‘Qinguan’Apple[J]. ACTA HORTICULTURAE SINICA, 2011, 38(7): 1341-1348.

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Studies on Subcellular Localization and Expression in E.coli of MdWRKY from‘Qinguan’Apple

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