Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (2): 397-409.doi: 10.16420/j.issn.0513-353x.2021-1039

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Genome-wide Identification of GRAS Gene Family in Apple and Expression Analysis of Its Response to Auxin

YU Tingting, LI Huan, NING Yuansheng, SONG Jianfei, PENG Lulin, JIA Junqi, ZHANG Weiwei, YANG Hongqiang*()   

  1. State Key Laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China
  • Received:2022-06-06 Revised:2022-09-26 Online:2023-02-25 Published:2023-03-06
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GRAS is a plant-specific transcription factor. In this study,78 GRAS genes were identified from the whole genome of apple(Malus × domestica)based on the conserved domain PF0351. MdGRASs encoding proteins belong to 11 subfamilies and most of them are the members of LISCL subfamily. They are located on 18 chromosomes,11 of which are located on chromosome 3. There were segmental duplication and tandem duplication events in GRAS family of apple. Ogran-specific expression profile analysis showed that,except for MdGRAS47,most of MdGRASs exhibited low transcript abundance levels in apple roots;MdGRAS20 showed higher expression level in shoots while MdGRAS18MdGRAS55 and MdGRAS61 highly expressed in seeds. Promoter analysis indicated that all 78 MdGRASs contained light response elements,76 out of 78 MdGRASs contained stress-related elements and 37 out of 78 MdGRASs contained auxin response elements. It was confirmed that 17 MdGRASs could respond to 2,4-D with MdGRAS30 and MdGRAS61 responded to 2,4-D most significantly.

Key words: apple, GRAS gene family, bioinformatics, promoter, auxin

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