Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (8): 1621-1636.doi: 10.16420/j.issn.0513-353x.2021-0545

• Research Papers •     Next Articles

Bioinformatics Analysis of Apple ELO Gene Family and Its Expression Analysis Under Low Temperature Stress

GAO Yanlong, WU Yuxia, ZHANG Zhongxing, WANG Shuangcheng, ZHANG Rui, ZHANG De, WANG Yanxiu*()   

  1. College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China
  • Received:2022-04-27 Revised:2022-06-06 Online:2022-08-25 Published:2022-09-05
  • Contact: WANG Yanxiu


In order to study the role of apple ELO gene family in wax synthesis and cold resistance,bioinformatics methods were used to identify and analyze the ELO family members in the whole apple genome. The results showed that the MdELO gene family consisted of seven members,which were unevenly distributed on three apple chromosomes(Chr2,Chr8 and Chr15). The MdELO protein contains 211-305 amino acid residues with isoelectric points ranging from 9.43 to 11.62. The results of subcellular localization showed that MdELO protein was distributed in the nucleus,cell membrane and chloroplast. The analysis of cis-acting elements showed that the 2 000 bp sequences upstream of the seven MdELO promoters were distributed with response elements such as hormones,environmental adaptability and stress induction. The wax content in different apple cultivars was further determined,and the results showed that the wax content of‘Oregon Spur Delicious’was significantly higher than that of‘Miyazaki’;On the other hand,from the it perspective of wax morphology and cuticle morphology,the wax of‘Oregon Spur Delicious’was needle-like and closely distributed,and the cuticle structure of its branches was close and smooth,while the wax of‘Miyazaki’was loose and lumpy,and the cuticle structure of its branches was loose and the surface was rough. Additionally,the proline,relative electrical conductivity and starch phosphorylase of the two apple branches increased firstly and then decreased with the extension of the overwintering period,and the amylase increased gradually,among which the content and activity of all indexes of‘Oregon Spur Delicious’were higher than that of‘Miyazaki’. The changes of the above indexes showed that the cold resistance of‘Oregon Spur Delicious’was significantly higher than that of‘Miyazaki’. It was found that the expression of ELO family members can be detected in the roots and leaves through qRT-PCR analysis,and the expression level displayeded certain differences. Most of them showed a trend of rising first and then falling,and the expression level is the highest at 12 h or 24 h,indicating that this family members may play an important role in low temperature stress.

Key words: apple, ultra long chain fatty acid, ELO gene family, bioinformatics analysis, expression analysis, cold resistance

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