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Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (9): 1883-1894.doi: 10.16420/j.issn.0513-353x.2020-0707

• Research Papers • Previous Articles     Next Articles

Cloning and Functional Analysis of PgMYB111 Related to Anthocyanin Synthesis in Pomegranate

WANG Sha, ZHANG Xinhui, ZHAO Yujie, LI Bianbian, ZHAO Xueqing, SHEN Yu, DONG Jianmei, YUAN Zhaohe*()   

  1. Co-Innovation Center for Sustainable Forestry in Southern China,College of Forestry,Nanjing Forestry University,Nanjing 210037,China
  • Received:2022-04-13 Revised:2022-06-14 Online:2022-09-25 Published:2022-10-08
  • Contact: YUAN Zhaohe E-mail:zhyuan88@hotmail.com

Abstract:

To study the regulatory mechanism of the anthocyanin synthesis pathway in pomegranate,we isolated PgMYB111 gene from the petals of‘Liuhuahong’and‘Liuhuabai’. Results showed that the cDNA sequence contained a 1 101 bp open reading frame(ORF)encoding a 366 amino acid polypeptide. Multiple alignment analysis of amino acid sequences showed that the N-terminus of PgMYB111 protein contained a conserved R2R3 domain and a binding site of [D/E]Lx2[R/K]x3Lx6Lx3R interacted with bHLH protein. Furthermore,the amino acid sequence had the highest similarity with MYB transcription factors in Malus × domestica and Prunus dulcis. Subcellular localization assays suggested that PgMYB111 localized in the nucleus. qRT-PCR analysis indicated that the expression of PgMYB111 gene in‘Liuhuahong’ was 4.3 times higher than that in‘Liuhuabai’. In addition,the anthocyanin content in‘Liuhuahong’petal is significantly higher than that in‘Liuhuabai’petal. The results of tobacco transient expression showed that the expression level of the gene increased at first and then decreased. The anthocyanin content in transgenic tobacco leaves was consistent with the gene expression,and reached the highest level on the fifth day. They were 3.8 times and 8.9 times higher than those in uninfected leaves,and 3.17 times and 5.57 times higher than those in pBI121 leaves,respectively. In summary,PgMYB111 could involve in the anthocyanin synthesis pathway and facilitate its synthesis.

Key words: pomegranate, anthocyanin, gene cloning, subcellular localization, flower colour

CLC Number: