Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (9): 1883-1894.doi: 10.16420/j.issn.0513-353x.2020-0707
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WANG Sha, ZHANG Xinhui, ZHAO Yujie, LI Bianbian, ZHAO Xueqing, SHEN Yu, DONG Jianmei, YUAN Zhaohe*()
Received:
2022-04-13
Revised:
2022-06-14
Online:
2022-09-25
Published:
2022-10-08
Contact:
YUAN Zhaohe
E-mail:zhyuan88@hotmail.com
CLC Number:
WANG Sha, ZHANG Xinhui, ZHAO Yujie, LI Bianbian, ZHAO Xueqing, SHEN Yu, DONG Jianmei, YUAN Zhaohe. Cloning and Functional Analysis of PgMYB111 Related to Anthocyanin Synthesis in Pomegranate[J]. Acta Horticulturae Sinica, 2022, 49(9): 1883-1894.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2020-0707
注释 Annotation | 引物 Primer | 引物序列(5′-3′) Primer sequence |
---|---|---|
基因克隆 Gene clone | PgMYB111 | F:ATGGGGAGGAAACCAT;R:TCAAGTTTTAGTGATCTTCTC F:gagaacacgggggactctagaATGGGGAGGAAACCAT R:gcccttgctcaccatggatccTCAAGTTTTAGTGATCTTCTC |
亚细胞定位 Subcellular localization | GFP-PgMYB111 | |
基因表达 Gene expression | PgMYB111 | F:ATCTCTCCCCAAGAATGCC;R:AGACATGACGCTGTTACCCAA |
PgCHS | F:AGCGGACTACAAGCTCA;R:CACTCGCGCATCAGCA | |
PgDFR | F:CCGGCATCGCAAAGCTC;R:CACAGCCCCGACGAAC | |
PgUFGT | F:CGCCCATATCTACACTGACC;R:AGTCCAAATCGCCGAAG | |
PgANS | F:CCTATGCAAACTCCCCGAA;R:TGCTTCCCGTACTCGTTG | |
PgActin | F:AGTCCTCTTCCAGCCATCTC;R:CACTGAGCACAATGTTTCCA |
Table 1 Primers for the gene cloning,subcellular localization and qRT-PCR
注释 Annotation | 引物 Primer | 引物序列(5′-3′) Primer sequence |
---|---|---|
基因克隆 Gene clone | PgMYB111 | F:ATGGGGAGGAAACCAT;R:TCAAGTTTTAGTGATCTTCTC F:gagaacacgggggactctagaATGGGGAGGAAACCAT R:gcccttgctcaccatggatccTCAAGTTTTAGTGATCTTCTC |
亚细胞定位 Subcellular localization | GFP-PgMYB111 | |
基因表达 Gene expression | PgMYB111 | F:ATCTCTCCCCAAGAATGCC;R:AGACATGACGCTGTTACCCAA |
PgCHS | F:AGCGGACTACAAGCTCA;R:CACTCGCGCATCAGCA | |
PgDFR | F:CCGGCATCGCAAAGCTC;R:CACAGCCCCGACGAAC | |
PgUFGT | F:CGCCCATATCTACACTGACC;R:AGTCCAAATCGCCGAAG | |
PgANS | F:CCTATGCAAACTCCCCGAA;R:TGCTTCCCGTACTCGTTG | |
PgActin | F:AGTCCTCTTCCAGCCATCTC;R:CACTGAGCACAATGTTTCCA |
材料 Meterial | 花色 Color | L* | a* | b* | C* | h |
---|---|---|---|---|---|---|
榴花红Liuhuahong | 红色Red | 54.35 ± 1.05 | 49.73 ± 1.18 | 31.42 ± 1.33 | 58.93 ± 0.97 | 32.42 ± 1.45 |
榴花白Liuhuabai | 白色White | 85.82 ± 0.92 | -1.14 ± 0.20 | 7.07 ± 0.29 | 7.16 ± 0.29 | -80.83 ± 1.45 |
Table 2 Date of CIE L*,a*,b* in petals of pomegranate
材料 Meterial | 花色 Color | L* | a* | b* | C* | h |
---|---|---|---|---|---|---|
榴花红Liuhuahong | 红色Red | 54.35 ± 1.05 | 49.73 ± 1.18 | 31.42 ± 1.33 | 58.93 ± 0.97 | 32.42 ± 1.45 |
榴花白Liuhuabai | 白色White | 85.82 ± 0.92 | -1.14 ± 0.20 | 7.07 ± 0.29 | 7.16 ± 0.29 | -80.83 ± 1.45 |
Fig. 1 Phenotype(A)and anthocyanin content(B)in full bloom of‘Liuhuabai’and‘Liuhuahong’flowers Different lowercase letters indicate significant differences at 0.05 level.
Fig. 3 Multiple sequence alignment of the proteins AtMYB111:Arabidopsis thaliana(NP_199744.1);CsMYB111:Citrus reticulata(XP_006488875.1);VvMYB111:Vitis vinifera(RVW39925.1);MdMYB111:Malus × domestica(XP_028951203.1);EgMYB111:Elaeis guineensis(XP_010921536.1);AhMYB111:Arachis hypogaea(XP_025692112.1);PdMYB111:Amygdalus communis(XP_034227785.1);PtMYB111:Populus trichocarpa(XP_002316017.2);BrMYB111:Brassica rapa(XP_009151728.1);AtMYB11:Arabidopsis thaliana(NP_191820.1);AtMYB12:Arabidopsis thaliana(ABB03913.1);AtMYB90:Arabidopsis thaliana(NP_176813.1);AtMYB75:Arabidopsis thaliana(ABB03879.1);AtMYB113:Arabidopsis thaliana(OAP11934.1);AtMYB114:Arabidopsis thaliana(AEE34502.1);AN2:Petunia hybrida(BAP28593.1).
Fig. 6 Expression of PgMYB111 in different tissues(A)and expression of PgMYB111(B),PgCHS(C),PgDFR(D),PgUFGT(E)and PgANS(F)in ‘Liuhuahong’ and ‘Liuhuabai’ Different lowercase letters indicate significant differences at 0.05 level. The same below.
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