Exploration and Regulation Network Analysis of CBF Pathway Related Cold Tolerance Genes in Lavandula angustifolia

doi:10.16420/j.issn.0513-353x.2021-0888

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (1): 131-144.doi: 10.16420/j.issn.0513-353x.2021-0888

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Exploration and Regulation Network Analysis of CBF Pathway Related Cold Tolerance Genes in Lavandula angustifolia

LIN Haijiao¹, LIANG Yuchen¹, LI Ling¹, MA Jun², ZHANG Lu¹, LAN Zhenying¹, YUAN Zening¹^,^*()

¹Key Laboratory of Biodiversity of Aquatic Organisms，Harbin Normal University，Harbin 150025，China
²Industrial Crops Institute，Heilongjiang Academy of Agricultural Sciences，Harbin 150086，China

Received:2022-07-25 Revised:2022-12-06 Online:2023-01-25 Published:2023-01-18
Contact: *（E-mail：xiaoyuan168ok@163.com）

Abstract

Abstract:

To explore the cold-tolerant mechanisms of lavender（Lavandula angustifolia Mill.），transcriptomics methods were used for discovering and analyzing the molecular regulation networks in responses to cold treatments in this research. The CBF-COR regulation networks composed by CBF transcription factors and their target genes COR played significant roles in improving plant cold-tolerance. In this research，seven CBF genes（LaCBF）and 11 COR genes（LaCOR）were found with significant expression difference under cold stress. Meanwhile，four upstream genes including LaICE1，LaCAMTA3，LaEBF1，and LaCCA1 in CBF regulation pathways were found to be responsive to cold stress. It is considered that the cold signal regulation networks together formed by these genes took the key roles in resistance to low temperature in Lavandula angustifolia.

Key words: Lavandula angustifolia, low temperature stress, transcriptomics analysis, CBF transcription factors

CLC Number:

LIN Haijiao, LIANG Yuchen, LI Ling, MA Jun, ZHANG Lu, LAN Zhenying, YUAN Zening. Exploration and Regulation Network Analysis of CBF Pathway Related Cold Tolerance Genes in Lavandula angustifolia[J]. Acta Horticulturae Sinica, 2023, 50(1): 131-144.

Figures/Tables 10

Table 1 Primers used for qPCR

序号 ID	引物序列（5′-3′） Primers sequence
1	CCA1-F:TGCCGGGAAAGAGGAGTACT	CCA1-R:CCCACTTCGTTAGGAGGCAA
2	LHY-F:TGGGTAATTGGGTCAAGGCC	LHY-R:TTCCGGGTCTTTCTCCATGC
3	ICE1-F:TTGAGCTGCGACCAGAGTTT	ICE1-R:CAGCTTAGACCTGTGCGGAT
4	CBF1-F:TGCAGCGGAGGCTTTCTATC	CBF1-R:AAGCCTTCAGCCATGTTGGA
5	CBF2-F:CGATACAGGCAAGTGGGTCT	CBF2-R:AATGTCCTGCACACTCGACG
6	CBF3-F:GTGCTGGAATTCTCCTCCGA	CBF3-R:TGACCTCGCAGACCCACTTA
7	ERD7-F:TGGTGAGGAAGAAGGATGCG	ERD7-R:CCACTTCAGCCTATCCACCG
8	COR413PM2-F:AGAGGCGGGGTAGTCATCTT	COR413PM2-R:GTCAGTGCTCGTGTGTGAGA
9	GAPDH-F:TAGGAGGTGGCAGGACATCA	GAPDH-R:CCCTTTACCCGTCACGTTGT

Fig. 1 Volcano map of transcription factor differentially expressed genes in Lavandula angustifolia under 0 ℃ stress

Fig. 2 GO statistical bar chart of transcription factor differentially expressed genes in Lavandula angustifolia

Fig. 3 Cluster map of cold tolerance genes associated with CBF-COR regulatory network in Lavandula angustifolia

Fig. 4 GO chordal graph of cold tolerance genes associated with CBF-COR regulatory network in Lavandula angustifolia

Fig. 5 Mapping of protein network interactions between CBF pathway related cold tolerance genes in Lavandula angustifolia and Arabidopsis thaliana database

Fig. 6 Phylogenetic tree of CBF transcription factors in Lavandula angustifolia and Arabidopsis thaliana

Fig. 7 Changes of COR gene expression in Lavandula angustifolia at different temperatures Different letters indicated significant difference（P < 0.05）.

Fig. 8 Comparison of gene expression levels between RNA-seq and qPCR

Fig. 9 CBF-COR regulatory network in response to low temperature stress in Lavandula angustifolia Modified and drawn according to the literature Shi et al.（2018）.

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Exploration and Regulation Network Analysis of CBF Pathway Related Cold Tolerance Genes in Lavandula angustifolia

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