Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (1): 177-187.doi: 10.16420/j.issn.0513-353x.2021-0822

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Comparative Transcriptome Analysis of Differential Expression in Different Tissues of Corydalis yanhusuo

ZHAO Xueyan1, WANG Qi2, WANG Li1, WANG Fangyuan1, WANG Qing1, LI Yan1,*()   

  1. 1Shaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources,Xi’an Botanical Garden of Shaanxi Province,Institute of Botany of Shaanxi Province,Xi’an 710061,China
    2School of Pharmacy,Shaanxi Institute of International Trade and Commerce,Xianyang,Shaanxi 712046,China
  • Received:2021-11-08 Revised:2022-06-03 Online:2023-01-25 Published:2023-01-18
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The dried tubers of Corydalis yanhusuo can be used as the traditional Chinese medicine,and isoquinoline alkaloid compounds are the main active ingredient. To identify genes related to isoquinoline alkaloid biosynthesis and analyze the differentially expressed genes of tuber,rhizome and leaf tissues of C. yanhusuo,transcriptome sequencing of these tissues using Illumina high-throughput sequencing technology were performed. A total of 6 161,8 852 and 5 772 differentially expressed genes were identified in comparisons of tuber vs. rhizome,tuber vs. leaf and rhizome vs. leaf,respectively.KEGG enrichment analysis of differentially expressed genes indicated that major enrichment pathways were starch and sucrose metabolism,phenylpropanine biosynthesis,plant hormone signal transduction and isoquinoline alkaloid biosynthesis pathways. Fourty-seven transcripts were identified to be related to isoquinoline alkaloid biosynthesis and the highest level of gene expression was found in tuber,following by rhizome and leaf.

Key words: Corydalis yanhusuo, isoquinoline alkaloids, transcriptome, expression analysis

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