Differential Expression Analysis of the Fruits of Yellow-fleshed Apple and Exploits Genes Involved in Carotenoid Pigmentation

doi:10.16420/j.issn.0513-353x.2016-0449

ACTA HORTICULTURAE SINICA ›› 2017, Vol. 44 ›› Issue (2): 205-214.doi: 10.16420/j.issn.0513-353x.2016-0449

Differential Expression Analysis of the Fruits of Yellow-fleshed Apple and Exploits Genes Involved in Carotenoid Pigmentation

SONG Chengxiu，ZHANG Liyi*，ZHANG Caixia，TIAN Yi，and CONG Peihua

Research Institute of Pomology，Chinese Academy of Agricultural Sciences；Key Laboratory of Biology and Genetic Improvement of Horticultural Crops（Germplasm Resources Utilization），Ministry of Agriculture，P. R. China，Xingcheng，Liaoning 125100，China

Online:2017-02-25 Published:2017-02-25

Abstract

Abstract:

The transcriptome of the fruit of a yellow-fleshed apple genotype，‘Dongbei Huanghaitang’in two developing stages（90 and 115 days after full bloom）were compared using RNA-seq，in order to understand the dynamics and differential expression of genes ，including 1 786 genes significantly down-regulated and 1 270 genes significantly up-regulated. These differences genetic gene were noted by Pathway enrichment analysis. A set of 7 differentially expressed genes，enriching in carotenoids metabolic pathways was identified，including 4 genes significantly down-regulated and 3 genes significantly up-regulated in two development stages. A new CCD candidate gene，MdCCD4b，was further found by the methods of Quantitative RT-PCR and evolutionary clustering analysis. It was putatively assumed that the down-regulated expression of MdCCD4b in ripe period likely results in carotenoid accumulation in yellow-fleshed genotype，in that the MdCCD4b had a negative correlated with carotenoid accumulation in apple fruit. These findings lay a basis for further investigation into the molecular mechanism underlying carotenoid accumulation in yellow-fleshed apple.during fruit development and ripening. The results show that the two samples have 3 056 differentially expressed genes

Key words: apple, yellow-flesh, transcriptome, carotenoid, MdCCD4

CLC Number:

S 661.1

SONG Chengxiu，ZHANG Liyi*，ZHANG Caixia，TIAN Yi，and CONG Peihua. Differential Expression Analysis of the Fruits of Yellow-fleshed Apple and Exploits Genes Involved in Carotenoid Pigmentation[J]. ACTA HORTICULTURAE SINICA, 2017, 44(2): 205-214.

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Differential Expression Analysis of the Fruits of Yellow-fleshed Apple and Exploits Genes Involved in Carotenoid Pigmentation

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