Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (7): 1415-1428.doi: 10.16420/j.issn.0513-353x.2021-0450

• Research Papers • Previous Articles     Next Articles

Identification and Analysis of Apple Viruses Diseases Based on Virome Sequencing Technology

XIA Yan1, HUANG Song1,2, WU Xueli1, LIU Yiqi1, WANG Miaomiao1, SONG Chunhui1, BAI Tuanhui1, SONG Shangwei1, PANG Hongguang1, JIAO Jian1(), ZHENG Xianbo1()   

  1. 1College of Horticulture,Henan Agricultural University,Zhengzhou 450002,China
    2Xinyang Agriculture and Forestry University,Xinyang,Henan 464001,China
  • Received:2022-03-11 Revised:2022-05-30 Online:2022-07-25 Published:2022-07-29


Virome Sequencing Technology was used for virus detection and analysis of virus-infected apple trees in Henan Province,a total of 13 viruses were detected from apple leaves in the sequenced database,including five apple viruses:apple stem grooving virus(ASGV),apple stem pitting virus(ASPV),apple chlorotic leaf spot virus(ACLSV),apple necrotic mosaic virus(ApNMV)and apple green crinkle associated virus(AGCaV). RT-PCR was used to verify the detection results of five apple viruses,and objective bands were obtained after amplification,it was consistent with virome sequencing results,indicating that the virome sequencing detection results were accurate and reliable. Based on the sequencing results,specific primers were designed to amplify the full length of ASPV,a length of 9 246 nt ASPV-HN isolate genome was obtained and shared sequence identities 75.48% to 79.85% with complete genome of 19 ASPV isolates published in NCBI database;the two assembly sequences of ASGV genomes ASGV-HN1 and ASGV-HN2 based on virome sequencing results were both 6 475 nt in length and shared sequence identities 79.98% to 98.08% with complete genome of 18 ASGV isolates published in NCBI database. Detection of apple mosaic virus(ApMV)and ApNMVwere carried out in 53 apple leaf samples collected from different apple producing areas in Henan Province. ApNMV was detected in all samples with mosaic disease,while ApMV was not detected in 53 samples. It indicated that ApNMV may be the main pathogen which can cause apple mosaic disease in Henan Province.

Key words: apple, virus disease, Virome Sequencing Technology, RT-PCR, detection, apple mosaic disease

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